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  • gene expression
  • found that, although Tcf/Lef transcription factors promoted gene expression by cooperating with β-catenin in response to Wnt signaling in proliferating myoblasts, β-catenin was not required for the induction of Tcf/Lef target genes during quiescence. (sciencemag.org)
  • Depletion of Smad3, but not β-catenin, reduced Lef1 occupancy at target promoters, Tcf/Lef target gene expression, and self-renewal of myoblasts. (sciencemag.org)
  • MSCs
  • By employing microarray, expressed sequence tag (EST) followed by RNA-Seq techniques to MSCs satellite cell analysis, we were able to delineate the regulatory network of genes corresponding to different transcription factors and certain prominent members of the extracellular protein family, involved in controlling myoblast differentiation [20, (neuroart2006.com)
  • induction
  • Scientists demonstrated that induction of the transcription factor CCAAT/enhancer-binding protein β (C/EBPβ) using the cyclic adenosine monophosphate phosphodiesterase inhibitor isobutylmethylxanthine (IBMX) results in enhanced myoblast expansion in culture and increased satellite cell marker expression. (musclecellnews.com)
  • Entry of cultured myoblasts into G 0 is associated with a specific, reversible induction of Wnt target genes, thus implicating members of the Tcf and Lef1 (Tcf/Lef) transcription factor family, which mediate transcriptional responses to Wnt signaling, in the initiation of quiescence. (sciencemag.org)
  • fibroblasts
  • In order to characterize the effects of post-transcriptional control on the quiescent transcriptome in human fibroblasts, we determined mRNA decay rates for over 10,000 genes using a transcription shut-off time-course. (biomedcentral.com)