AssaysPolymerase Chain ReAssayAntibodiesTiterSerologicalBovineDetectionSerumELISAAPTTVaccinationCoagulationSerologicHigh-throughputLaboratoryPlateletInterferenceRecombinantTitersNeutralizeAntibody responsesMeasurementSpikeAnalysisDiagnosticSera were testedMolecularPlasmaExaminationClinicalCellsResultsParticlesResearchersFundamentalEssentialSamplesAssessSolidsValidationInternationalEvaluateApproach
Assays5
- The current Food and Drug Administration -approved serological tests do not measure antibody-mediated viral neutralization, and there is a need for standardized quantitative neutralization assays. (bvsalud.org)
- Some preliminary neutralization assays have been carried out by these authors. (datexis.com)
- In this setting, antigen rapid tests yielded an overall sensitivity of 83.3% (not significantly different between the four assays) while the use of automated antigen dosing would have spared 93.5% of asymptomatic inpatient screening PCRs. (frontiersin.org)
- Nineteen national-level public health laboratories performed routine dengue diagnostic assays on a proficiency testing panel consisting of two modules: one containing commercial serum samples spiked with cultured dengue viruses for the detection of nucleic acid and non-structural protein 1 (NS1) (Module A) and one containing human serum samples for the detection of anti-dengue virus antibodies (Module B). A review of logistics arrangements was also conducted. (who.int)
- Recently, standard procedures were adopted for conducting neutralization assays. (who.int)
Polymerase Chain Re2
- Interferon γ (IFN-γ) and interleukin (IL)-4 RNA expression was examined in T lamina propria lymphocytes by quantitative reverse transcription-polymerase chain reaction. (bmj.com)
- All 16 laboratories testing Module A performed reverse transcriptase polymerase chain reaction (RT-PCR) for both RNA and serotype detection. (who.int)
Assay11
- Although each national laboratory used its preferred assay, the results were all standardized to those of the in vitro neutralization test and expressed in international units (IU) which allowed comparative analyses to be performed. (cambridge.org)
- Screening and downselection for serum antibody binding to the receptor-binding domain are followed by quantitative neutralization testing using a chimeric vesicular stomatitis virus expressing spike protein of SARS-CoV-2 in a real- time cell analysis assay. (bvsalud.org)
- 28 reactive samples were used to compare antibody levels with a pseudotyped neutralization assay. (degruyter.com)
- However, because no assay is 100% specific, serologic testing of non-measles cases using any assay will occasionally produce false positive IgM results. (cdc.gov)
- In some cases, vaccination is not possible, and testing with a second line diagnostic assay may be necessary to determine the patient's immune status. (cdc.gov)
- A commercial, indirect EIA (non-quantitative) assay is used for detection of IgG. (cdc.gov)
- Hence, the development of an in vitro neutralization assay for HCV could be extremely valuable for characterizing the humoral immune response to HCV and for evaluating the potential of passive and active immunization against hepatitis C. Recent studies using an in vitro neutralization assay system (based on infectious retroviral pseudoparticles (HCVpp) bearing HCV envelope glycoproteins) have confirmed that HCV-infected patient sera can indeed neutralize infection. (datexis.com)
- In this study, we describe how we set up a standardized focus reduction neutralization assay based on HCVcc. (datexis.com)
- GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunosorbent assay which can be used for quantitative detection of cAMP (Adenosine 3', 5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. (genscript.com)
- Laboratory testing with a rapid conducted at that time showed that 6.1% of the residents assay suggested that a dengue virus (DENV) was the caus- in nearby regions of Uganda had specifi c antibodies to ative agent. (folkhalsomyndigheten.se)
- The 'gold standard' method to assess humoral antibody responses fol owing vaccination is the neutralization assay. (who.int)
Antibodies5
- For each country, a nationally representative population sample was collected across the entire age range and was tested for antibodies to diphtheria toxin. (cambridge.org)
- The antibody responses against SARS-CoV-2 in humans are induced by some viral proteins, including spike glycoprotein (S protein) and nucleocapsid protein, among which S protein can induce neutralizing antibodies that are indispensable for viral neutralization and elimination, through blocking viral binding with host cells 1 . (nature.com)
- Although highly homologous amino acid sequences are shared between the RBD regions of SARS-CoV-2 and SARS-CoV-1, the plasma of convalescent SARS patients or SARS-CoV-1 RBD monoclonal antibodies could not neutralize SARS-CoV-2, indicating the limited cross-neutralization protection between these two viruses 5 , 7 . (nature.com)
- The IgG antibodies to SARS CoV-2 directed against the spike protein (S-antigen, both S1 and S2 protein) were assayed with LIASON® S1/S2 quantitative antibody detection kit (DiaSorin Saluggia, Italy) using indirect chemiluminescence immunoassay (CLIA). (cmeindia.in)
- The performance of the nanoribbon biosensor upon the use of two different types of molecular probes - the antibodies and the aptamers against HCVcoreAg - has been tested. (preprints.org)
Titer1
- GenScript AAV2 Titer Capsid ELISA Kit is a Sandwich ELISA Kit that is designed for quantitatively measuring the total capsid titer of adeno-associated virus serotype 2 (AAV2) in testing samples during viral vector production. (genscript.com)
Serological2
- To regulate the international movement of domestic carnivores from infected countries to free territories from rabies, a system combining the anti-rabies vaccination of the animal and serological testing was launched. (researchsquare.com)
- This first round of external quality assessment of dengue diagnostics was successfully conducted in national-level public health laboratories in the WHO Western Pacific Region, revealing good proficiency in both molecular and serological testing. (who.int)
Bovine1
- GenScript BSA ELISA Kit is a Sandwich ELISA kit that is designed for quantitative measurement of bovine serum albumin (BSA) in testing samples. (genscript.com)
Detection5
- GenScript Human IL-8 ELISA Detection Kit is a solid phase sandwich ELISA for quantitative measurement of human IL-8 in serum, plasma and cell culture supernatant. (genscript.com)
- We then established a diagnostic algorithm by approaching median viral loads in target populations and evaluated the limit of detection of each test using the PCR cycle threshold values. (frontiersin.org)
- Since then, a new set of diagnostic tools have been developed, such as antigen detection immunoassays or molecular point-of-care tests. (frontiersin.org)
- Thanks to their high sensitivity, ranging from 73.9 to 89.5% for high viral load samples [10 5 -10 7 RNA copies/swab ( 3 )], and their overall specificity ( 4 , 5 ), antigen-detection rapid diagnostic tests have been integrated in several countries' testing strategies ( 6 - 10 ) 1 , 2 . (frontiersin.org)
- 1] The dilute Russel viper venom test (dRVVT) is widely used as another coagulation test for this purpose and is believed to be more specific for LA detection. (medscape.com)
Serum3
- Serologic tests can also result in false-negative results when serum specimens are collected too early with respect to rash onset. (cdc.gov)
- To expedite confirmation of measles from highly suspicious cases (recent travel, classic measles signs and symptoms), particularly in a setting with many potential exposures, an aliquot of serum, and either a nasopharyngeal or throat swab can be sent to CDC while testing is underway at the SPHL or local clinical laboratory. (cdc.gov)
- The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. (thermofisher.com)
ELISA1
- Evaluation of post-vaccination immunity against rabies was undertaken between 2003 and 2009 by an immunoenzymatic test Platelia Rabies II ELISA Kits (Bio-Rad). (researchsquare.com)
APTT4
- When such a technique is used, the test is referred to as the LA-sensitive aPTT or aPTT-LA. Other authors argue that a single aPTT using a reagent that is not optimally sensitive may suffice to diagnose LA, provided that its positivity is documented on 2 separate occasions. (medscape.com)
- 4] At present, most laboratories have adopted increasing the sensitivities of their standard aPTT tests such that the two have become, in general, interchangeable. (medscape.com)
- Regardless of the aPTT reagent used, interpretation of a prolonged aPTT in evaluation of LA activity must involve further tests to fulfill the aforementioned mentioned criteria to confirm the presence of LA. (medscape.com)
- The first method involves accentuating prolonged coagulation by reducing the amount of phospholipid, as is done in the tissue thromboplastin inhibition (TTI) test and the dilute aPTT test. (medscape.com)
Vaccination3
- RÉSUMÉ En 2001, le programme de vaccination antirougeoleuse en Arabie saoudite est passé à deux doses du vaccin antirougeoleux-antiourlien-antirubéoleux, la première à 12 mois et la seconde à 6 ans. (who.int)
- Le programme de vaccination antirougeoleuse en deux doses peut induire une protection optimale à l'entrée à l'école primaire lorsqu'une forte couverture vaccinale est assurée. (who.int)
- Defining surrogate serologic tests with respect to predicting protective vaccine efficacy: Poliovirus vaccination. (who.int)
Coagulation2
- Screening with 2 or more phospholipid-dependent coagulation tests and demonstrating prolongation of at least one. (medscape.com)
- 5] Other phospholipid-dependent coagulation tests include the Kaolin clotting test (KCT) and the dilute prothrombin time, both of which are not recommended by the ISTH due to poor reproducibility and reagent variability. (medscape.com)
Serologic4
- Subsequent serologic specimens sent for measles IgM testing may result in a false positive test. (cdc.gov)
- Blood for serologic testing of measles at CDC is collected as described in the Infectious Disease Laboratories Test Directory entry for each test, listed below. (cdc.gov)
- Blood for serologic testing of infants or small children can be collected by finger/heel stick. (cdc.gov)
- Requests for measles serologic testing that are strictly for rule-out purposes (low index of suspicion, does not meet clinical case definition, no travel or contact with cases), should be directed to either SPHLs or clinical laboratories. (cdc.gov)
High-throughput1
- Some of the commercial, manually-processed EIAs are slightly more sensitive than high-throughput automated IgG test platforms that are typically used by large commercial laboratories. (cdc.gov)
Laboratory10
- Laboratory evidence of immunity is based on testing to detect measles IgG. (cdc.gov)
- During outbreaks of measles, the CDC measles laboratory can assist with testing. (cdc.gov)
- With any of the prescribed methods, test accuracy is affected by a number of factors including cleanliness of the test apparatus, agitation of the fluid (required to resuspend solids) and the accidental contamination of the fluid by dirty containers and laboratory glassware. (machinerylubrication.com)
- In that context, from 2003 to 2009, 119 cats sera and 408 dogs sera were tested in our referal laboratory in Ivory Coast. (researchsquare.com)
- Samples were packed and brought to the virology laboratory for testing under local regulations. (researchsquare.com)
- It is a unit of the National Laboratory for the Development of Agriculture (LANADA). (researchsquare.com)
- The probability of contaminating the samples in non-separated laboratory units is very high. (fml-dubai.com)
- An analytical performance evaluation of four antigen rapid tests, one automated antigen dosing and one molecular point-of-care test was performed on samples sent to our laboratory for a SARS-CoV-2 reverse transcription PCR. (frontiersin.org)
- tory testing to the Arbovirus Diagnostic Laboratory at the Over the next 20 years, several ZIKV isolates were Centers for Disease Control and Prevention (CDC, Fort obtained from Aedes spp. (folkhalsomyndigheten.se)
- Accurate laboratory testing is a critical component of dengue surveillance and control. (who.int)
Platelet1
- The second and more sensitive method is to neutralize the inhibitor activity by increasing the amount of phospholipid, as is done in the platelet neutralization procedure (PNP) or the rabbit brain neutralization procedure (RBNP). (medscape.com)
Interference1
- Blood collection by using vacutainers after strong and long venous congestion may cause hemolysis (interference with a significant number of tests). (fml-dubai.com)
Recombinant2
- A capture IgM EIA (non-quantitative) that incorporates a recombinant measles virus nucleocapsid protein as the antigen is used to detect measles IgM. (cdc.gov)
- Two different HCVcoreAg preparations have been tested: recombinant b-galactosidase-conjugated HCVcoreAg ("Virogen", USA) and recombinant HCVcoreAg ("Vector-Best", Russia). (preprints.org)
Titers2
- Anti-RBD IgG (RBD-IgG) titers have been shown to be strongly and positively correlated with virus neutralization 6 . (nature.com)
- Post-vaccine sera mediated strong neutralization of live Victoria infection and although neutralization titers were reduced 14-fold against the P.1 variant first discovered in Brazil they remained largely effective. (elifesciences.org)
Neutralize1
- This approach enables a predictive screening process for identifying plasma units that neutralize SARS-CoV-2 . (bvsalud.org)
Antibody responses1
- To investigate the duration of humoral immune response in convalescent coronavirus disease 2019 (COVID-19) patients, we conduct a 12-month longitudinal study through collecting a total of 1,782 plasma samples from 869 convalescent plasma donors in Wuhan, China and test specific antibody responses. (nature.com)
Measurement3
- Testing of IFT usually calls for a 30-second aging of the interface before measurement begins. (machinerylubrication.com)
- Units for measurement, system of units-S.I., fundamental and derived units, measurements-errors in measurement-significant figures, dimensions-dimensional analysis-applications. (soaneemrana.org)
- When visual acuities are acceptable (better than 20/40), perform the cover test perform the cover test at 6 meters and 40 cm using loose prisms when necessary for accurate measurement. (berkeley.edu)
Spike1
- The lower and upper limit of this quantitative spike antibody kit is 3.8 and 400 AU/mL respectively, as per manufacture's brochure. (cmeindia.in)
Analysis8
- Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
- In all of my years in oil analysis, I cannot recall a single time when data from a surface tension (ST) or interfacial tension test (IFT) appeared on a routine analytical report, aside from transformer oil analysis, for which the test is considered routine. (machinerylubrication.com)
- Applications Tested: This RPA-T4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
- However, some liquids (i.e. highly caustic or highly acidic) may require predilution or neutralization before analysis. (elementalanalysis.com)
- If quantitative analysis is to be assured, then both of these factors must be accurately known. (elementalanalysis.com)
- Since instrument calibration is performed at a specific proton energy, knowledge of the proton energy loss is essential for quantitative analysis. (elementalanalysis.com)
- The significant point here is that the factors necessary to make these corrections are non-linear energy dependent functions, and therefore the data reduction computer code must incorporate these corrections in order to achieve quantitative analysis. (elementalanalysis.com)
- Based on quantitative Western blot analysis, only two of the 45 (4.4%) Pandemrix-vaccinated narcoleptic patients showed specific antibody response against the NS1 protein from the H1N1pdm09 virus, indicating past infection with the H1N1pdm09 virus. (plos.org)
Diagnostic4
- Our objective was to elaborate a diagnostic algorithm combining antigen rapid diagnostic tests, automated antigen dosing and rapid molecular tests and to assess its performance under routine conditions. (frontiersin.org)
- Our diagnostic algorithm proposed to test recently symptomatic patients using rapid antigen tests, asymptomatic patients using automated tests, and patients requiring immediate admission using molecular point-of-care tests. (frontiersin.org)
- However, the riches sources of biotin as egg yolk, leading to high biotin concentrations in samples, harming the accuracy of diagnostic and proteins concentrations tests. (preprints.org)
- Further comprehensive diagnostic testing for dengue virus and other priority pathogens in the Region will be assessed during future rounds. (who.int)
Sera were tested1
- Mouse and rabbit sera were tested to address the impact of the PC on DB immunogenicity. (asm.org)
Molecular1
- Using tests not considered the "gold standard" for COVID-19 diagnosis on well-defined target populations allowed for the optimization of their intrinsic performances, widening the scale of our testing arsenal while sparing molecular resources for more seriously ill patients. (frontiersin.org)
Plasma2
- Standardized two-step testing of antibody activity in COVID-19 convalescent plasma. (bvsalud.org)
- Once an abnormal screening test result for LA is identified, the next step is to document the presence of an inhibitor by mixing the patient's plasma with adding double-centrifuged normal plasma in a 1:1 proportion. (medscape.com)
Examination1
- National Testing Agenncy (NTA) Joint Entrance Examination (JEE (Main)-2023 Examination will be conducted in phases. (freejobalert.com)
Clinical3
- Clinical laboratories should have 50 or 100 µL capillary tubes that are typically used for a variety of tests such as hematocrits or total lipids on newborns. (cdc.gov)
- Various vaccine candidates have been developed and are currently tested in preclinical or clinical studies (reviewed in references 4 - 7 ). (asm.org)
- Only clinical faculty can make final decisions as to what tests are necessary to render appropriate clinical care. (berkeley.edu)
Cells2
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. (thermofisher.com)
- The neutralizing antibody response to PC-positive DBs was superior to that of PC-negative DBs, as tested on fibroblasts, epithelial cells, and endothelial cells and for both animal species used. (asm.org)
Results1
- This is based on evidence showing that no single test is sensitive for detecting all LA. However, the risk of false-positive results increases to an unacceptable level if more than 2 screening tests are performed. (medscape.com)
Particles1
- Omicron mutations enhance infectivity and reduce antibody neutralization of SARS-CoV-2 virus-like particles ' by Syed, A., et al. (asm.org)
Researchers1
- This book will be very resourceful for new entrepreneurs, existing units, technocrats, researchers and technical libraries. (niir.org)
Fundamental1
- Fundamental progress in these diverse fields and small-angle x-ray scattering (SAXS) studies (Lafont must ultimately be based on a quantitative understanding of et al. (lu.se)
Essential1
- Essential oils testing systems. (agric.za)
Samples1
- In addition, reactive samples with anti-S and anti-N and samples with only anti-S showed neutralization capabilities (11/14 and 8/14, respectively). (degruyter.com)
Assess1
- To assess the susceptibility of K18-hACE2 mice to SARS-CoV-2, we intranasally challenged male and female mice using inocula of 10 3 , 10 4 and 10 5 plaque-forming units (PFU). (nature.com)
Solids1
- Solid state: classification of solids, X -ray studies of crystal lattices & unit cell. (successcds.net)
Validation1
- We offer validation services, technology transfers, and lot release testing which are completed with oversight from our Quality Assurance Unit. (scientist.com)
International1
- Titre is expressed as Equivalent Units per ml (EU/ml), unit equivalent to the international units defined by seroneutralization test. (researchsquare.com)
Evaluate1
- Quoting from the book "Lubrication of Industrial and Marine Machinery" by William G. Forbes, "The interfacial tension test is the most valuable single test that can be used to evaluate a turbine oil . (machinerylubrication.com)
Approach1
- It is a cross-sectional, retrospective and documentary quantitative approach, from 2015 to 2016, held at the Regional Hospital of Juruá, located in the city of Cruzeiro do Sul, Acre, Brazil. (bvsalud.org)