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IsotopesIsotope LabelingNitrogen IsotopesPeptidesCarbon IsotopesOxygen IsotopesZinc IsotopesGlyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)DeuteriumMass SpectrometryGlyceraldehyde 3-Phosphate Dehydrogenase (NADP+)Peptide FragmentsIron IsotopesPeptide LibraryProteomicsChromatography, LiquidSulfur IsotopesIndicator Dilution TechniquesTandem Mass SpectrometryAmino Acid SequenceAntimicrobial Cationic PeptidesMolecular Sequence DataGas Chromatography-Mass SpectrometryPeptides, CyclicFood ChainKineticsPeptide MappingProteomeAmino AcidsRadioisotope Dilution TechniqueForensic SciencesProtein BindingOligopeptidesRadioactive TracersMummiesChromatography, High Pressure LiquidIntestinal AbsorptionRadiometric DatingAbsorptionNatriuretic Peptide, BrainProteinsAnimal MigrationCarbonDeuterium OxideModels, MolecularCell LineZinc RadioisotopesStrontium IsotopesBiological AvailabilityCalcium IsotopesBase SequenceEcosystemBinding SitesSpectrometry, Mass, Electrospray IonizationProtein ConformationReference StandardsDietModels, BiologicalVasoactive Intestinal PeptideOxidation-ReductionTime FactorsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationNitrogenHydrogenCalcitonin Gene-Related PeptideMagnetic Resonance SpectroscopyCell-Penetrating PeptidesMethaneGlyceraldehyde 3-PhosphateRNA, MessengerPeptide BiosynthesisProtein Structure, SecondaryRecombinant ProteinsCells, CulturedEscherichia coliReproducibility of ResultsPeptide YYTrypsinLeucinePeptide Nucleic AcidsCattleSubstrate SpecificityFeeding BehaviorPaleontologyElectrophoresis, Polyacrylamide GelWaterHairEcologyNatriuretic Peptide, C-TypeStructure-Activity RelationshipRadioisotopesFecesFeathersNeutron Activation AnalysisGeologic SedimentsHydrogen-Ion ConcentrationProtein Processing, Post-TranslationalMeteoroidsZincNatriuretic Peptides
Mass spectrometryProteomicsAminoELISAProteinsAnalysis
Mass spectrometry3
  • We investigated the entire set of proteins modulated by BAG3 silencing in the human anaplastic thyroid 8505C cancer cells by using the Stable-Isotope Labeling by Amino acids in Cell culture strategy combined with mass spectrometry analysis. (oncotarget.com)
  • Proteins in cells with or without DN R-Ras expression were differentially labeled with SILAC and mass spectrometry was used to identify phosphoproteins and determine their relative quantities in the presence and absence of DN R-Ras. (biomedcentral.com)
  • While the chemical reactivity of the protein backbone and side-chain residues is a proxy for protein conformation, coupling this reactivity to quantitative mass spectrometry is a challenge in complex environments. (bvsalud.org)
Proteomics2
  • We also examined the dataset of proteins obtained by the quantitative proteomics analysis using two tools, Downstream Effect Analysis and Upstream Regulator Analysis of the Ingenuity Pathways Analysis software. (oncotarget.com)
  • We apply a quantitative proteomics methodology to identify chloroacetanilide-destabilized proteins in HEK293T cells based on their binding to the H31Q mutant of the human Hsp40 chaperone DNAJB8. (bvsalud.org)
Amino2
  • A one-pot peptide bond-forming response has been developed utilizing unprotected amino acids and peptides. (n-egt.com)
  • Two totally different silylating reagents, HSi[OCH(CF 3 ) 2 ] 3 and MTBSTFA, are instrumental for the profitable implementation of this strategy, getting used for the activation and transient masking of unprotected amino acids and peptides at C -termini and N -termini, respectively. (n-egt.com)
ELISA1
Proteins1
  • Potential Siglec-E-interacting proteins were identified by proximity labeling in conjunction with proteomic analysis and confirmed by coimmunoprecipitation experiment. (biomedcentral.com)
Analysis2
  • By performing proximity labeling and proteomic analysis, we identified scavenger receptor CD36 as a cell surface protein interacting with Siglec-E. Further experiments performed in HEK293T cells transiently overexpressing Siglec-E and CD36 and peritoneal macrophages demonstrated that depletion of cell surface sialic acids by treatment with sialyltransferase inhibitor or sialidase did not affect interaction between Siglec-E and CD36 but retarded Siglec-E-mediated inhibition on oxidized LDL uptake. (biomedcentral.com)
  • the exact degree of labeling is indicated on the certificate of analysis for each product lot. (thermofisher.com)