Quantitative mass spectrometry. Medical search. Frequent questions

Here, we applied capillary electrophoresis time-of-flight mass spectrometry, which enables comprehensive and quantitative analysis of charged metabolites, to simultaneously measure their levels in tumor and grossly normal tissues obtained from 16 colon and 12 stomach cancer patients. (elsevierpure.com)
Trapped ion mobility spectrometry (TIMS) combined with quadrupole time of flight mass spectrometry (Q-TOF). (lu.se)

Nano-flow liquid chromatography tandem mass spectrometry (nano-flow LC-MS/MS) is the mainstay in proteome research because of its excellent sensitivity but often comes at the expense of robustness. (nature.com)
120,000 peptides in 16 h and sample multiplexing using tandem mass tags increases throughput to 11 proteomes in 16 h. (nature.com)
Tandem mass spectrometry, also known as MS/MS or MS2, is a technique in instrumental analysis where two or more mass analyzers are coupled together using an additional reaction step to increase their abilities to analyse chemical samples. (wikipedia.org)
Typical tandem mass spectrometry instrumentation setups include triple quadrupole mass spectrometers (QqQ), multi-sector mass spectrometer, quadrupole-time of flight (Q-TOF), Fourier transform ion cyclotron resonance mass spectrometers, and hybrid mass spectrometers. (wikipedia.org)
For tandem mass spectrometry in space, the different elements are often noted in a shorthand, giving the type of mass selector used. (wikipedia.org)
In tandem mass spectrometry in space, the separation elements are physically separated and distinct, although there is a physical connection between the elements to maintain high vacuum. (wikipedia.org)
By doing tandem mass spectrometry in time, the separation is accomplished with ions trapped in the same place, with multiple separation steps taking place over time. (wikipedia.org)
Tandem MS in space uses the coupling of two instrument components which measure the same mass spectrum range but with a controlled fractionation between them in space, while tandem MS in time involves the use of an ion trap. (wikipedia.org)
This experiment is commonly performed to identify transitions used for quantification by tandem MS. In a neutral loss scan, the first mass analyzer scans all the masses. (wikipedia.org)
In the present work an isocratic enantioselective high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the separation and quantitative determination of dextro - and levo -methorphan and their pharmacologically relevant metabolites, dextrorphan and levorphanol, respectively, in human blood samples. (univpm.it)
A sensitive, rapid, selective and accurate liquid chromatography coupled to quadrupole tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous identification and quantification of tamsulosin and finasteride in bulk and in their combined dosage form. (eurjchem.com)
Passive sampling (Chemcatcher® with a HLB receiving phase) together with high-resolution tandem mass spectrometry-targeted screening was used to provide high sensitivity and selectivity for the identification of a wide range of emerging pollutants in these urban waters. (springer.com)
Zhang Y, Wang Q, Zhang G, Jia W, Ren Y, Wu Y. Biomarker analysis of hemoglobin adducts of acrylamide and glycidamide enantiomers for mid-term internal exposure assessment by isotope dilution ultra-high-performance liquid chromatography tandem mass spectrometry. (cdc.gov)
A flow-injection analysis tandem mass spectrometry (FIA MSMS) method was developed for rapid quantitative analysis of 10 different inorganic and organic explosives. (mit.edu)
De novo peptide sequencing algorithms have been widely used in proteomics to analyse tandem mass spectra (MS/MS) and assign them to peptides, but quality-control methods to evaluate the confidence of de novo peptide sequencing are lagging behind. (arxiv.org)
This method is a quantitative procedure for the measurement of perchlorate in human urine using ion chromatography coupled with electrospray tandem mass spectrometry. (cdc.gov)

This study describes the use of the GERSTEL MPS robotic with automated liquid option for the analysis of pesticide residues in hemp oil samples by direct liquid injection gas chromatography/mass spectrometry (GC/MS). This technique is sensitive, accurate and precise and allows the quantification of pesticides of interest well below the established limits of quantification for the State of California. (gerstelus.com)
Peptides were labeled with iTRAQ reagents and quantified using 2-dimentional liquid chromatography (LC) electrospray (ES)-based mass spectrometry. (sdstate.edu)
Title : Quantitative Analysis of Menthol in Human Urine Using Solid Phase Microextraction and Stable Isotope Dilution Gas Chromatography/Mass Spectrometry Personal Author(s) : Huang, Wenlin;Blount, Benjamin C.;Watson, Clifford H.;Watson, Christina;Chambers, David M. (cdc.gov)
In this study, the reproducibility of tryptic digestion of complex solutions was investigated using liquid chromatography Fourier transform ion cyclotron resonance (LC FT-ICR) mass spectrometry. (lu.se)
O-(2.3,4.5.6-pentafluorobenzyl) hydroxylamine hydrochloride (PFBHA) has been used to analyze aldehydes in water because of its fast quantitative reaction to form oximes suitable for detection at the picogram (pg) level by gas chromatography/mass spectrometry (GCIMS) and gas chromatography/electron capture detection (GC/ECD). (cdc.gov)
The analytical method employed was gas-chromatography/mass spectrometry of head space samples. (erowid.org)
Practical parts include analysis of own or supplied samples by chromatography and mass spectrometry, with subsequent data analysis and identification of sample components. (lu.se)
Gas chromatography mass spectrometry is also available for the analysis of volatile compounds, as well as a state-of-the-art pipetting robot for automated sample preparation. (lu.se)

However, motif redundancy among detected peptides by mass spectrometry (MS) leads to errors and ambiguities in the analysis. (biorxiv.org)
This is a powerful analytical equipment used for identification of analyses in complex matrices as well as for quantitative and qualitative analysis of peptides and proteolytic digests. (lu.se)

These experimental observations provide a range for the up- and clown-regulations that are possible to study with electrospray ionization LC FT-ICR mass spectrometry. (lu.se)
The eluant from the column is ionized using an electrospray interface to generate and transmit negative ions into the mass spectrometer. (cdc.gov)

Recently, we presented the DirectMS1 method of ultrafast proteome-wide analysis based on minute-long LC gradients and MS1-only mass spectra acquisition. (sdu.dk)
A comprehensive characterization of brain and retina synaptic vesicle proteome by quantitative mass spectrometry. (mpg.de)

However, current proteomics workflows entail several manual sample preparation steps and are challenged by the microscopic mass-limited samples generated by LCM, impacting measurement robustness, quantification and throughput. (nih.gov)
Absolute quantification with mass spectrometry and isotope labelled internal standards has found broad applications in biomedical research. (nist.gov)

As the last installment of 'Mass Spectrometry Forum' stated (3): 'In a quantitative analysis using values measured with mass spectrometry [MS] - usually values associated with ion intensities - a nonlinear regression (weighted or nonweighted) might model the proportionality between measured instrument response and sample amount more accurately. (spectroscopyonline.com)
Urinary biomarkers for the diagnosis of cervical cancer by quantitative label-free mass spectrometry analysis. (cri.or.th)
Multiple stages of mass analysis separation can be accomplished with individual mass spectrometer elements separated in space or using a single mass spectrometer with the MS steps separated in time. (wikipedia.org)
The results of qualitative and quantitative analysis of some amphetamines and their analogs isolated from urine samples by solid phase micro-extraction with polydimethylsiloxane fibers are reported. (erowid.org)
HCP-mass spectrometry (HCP-MS) analysis is an orthogonal approach to enzyme-linked immunosorbent assay (ELISA) and can be used to achieve greater coverage and knowledge of the HCP profile of a biopharmaceutical. (bioprocessintl.com)
NREL uses dynamic secondary ion mass spectrometry (SIMS) for elemental analysis, depth profiling, interface analysis, and image analysis. (nrel.gov)
This study demonstrates the quantitative capabilities of coated blade spray (CBS) mass spectrometry (MS) for the concomitant analysis of multiple target substances in biofluid spots. (uwaterloo.ca)
In addition motif redundancy can affect quantitative and statistical analysis and prevent a realistic comparison of peptide numbers between datasets. (biorxiv.org)
The msCompare tool has previously demonstrated quantitative-proteomics analysis within Galaxy [ 6 ]. (biorxiv.org)
Automated proteomic sample preparation: The key component for high throughput and quantitative mass spectrometry analysis. (bvsalud.org)
This course covers modern mass spectrometry with a particular focus on the analysis of low molecular weight analytes. (lu.se)
For accurate quantitative or qualitative analysis, mass spectrometry is today combined with different chromatographic techniques. (lu.se)
Mass spectrometry for small molecules includes an overview of mass spectrometric techniques that can be used for analysis of low molecular weight analytes in both the gas and liquid phase. (lu.se)
Course organizer Peter Spégel Center for Analysis and Synthesis Department of Chemistry Lund University course gives theoretical and practical knowledge on how mass spectrometry can be used in qualitative and quantitative analyses of small molecules. (lu.se)
Chemical analysis of JUUL aerosols was conducted using selective ion flow tube mass spectrometry. (bvsalud.org)
Quantitative analysis of JUUL aerosol indicated that chemical constituents differ between JMi and JMa flavors. (bvsalud.org)
This sensitive and robust triple quadrupole mass spectrometer is used for analysis of low (ppb) levels of environmental compounds and biomarkers. (lu.se)

The molecules of a given sample are ionized and the first spectrometer (designated MS1) separates these ions by their mass-to-charge ratio (often given as m/z or m/Q). Ions of a particular m/z-ratio coming from MS1 are selected and then made to split into smaller fragment ions, e.g. by collision-induced dissociation, ion-molecule reaction, or photodissociation. (wikipedia.org)
These fragments are then introduced into the second mass spectrometer (MS2), which in turn separates the fragments by their m/z-ratio and detects them. (wikipedia.org)
Q-TOF mass spectrometer combines TOF and quadrupole instruments, which cause high mass accuracy for product ions, accurate quantitation capability, and fragmentation experiment applicability. (wikipedia.org)
Hybrid mass spectrometer consists of more than two mass analyzers. (wikipedia.org)
and BEBE - four-sector (reverse geometry) mass spectrometer. (wikipedia.org)
The mass spectrometer was operated in scheduled multiple reaction monitoring (MRM) mode, with four transitions for each dextromethorpan, levomethorphan, dextrorphan and dextromethorphan-d3 and two transitions for each levorphanol, levorphanol-d3 and dextrorphan-d3. (univpm.it)
MS/MS detection was carried out by monitoring the fragmentation of 408.74 → 227.29 ( m/z ), 373.11 → 304.96 ( m/z ) and 255.75 → 166.15 ( m/z ) for tamsulosin, finasteride and diphenhydramine (IS), respectively, on a triple quadrupole mass spectrometer. (eurjchem.com)
A variety of mass spectrometer types, including magnetic sector, quadrupole, time-of-flight, and ion trap, have and will continue to deepen our understanding of the formation and evolution of exploration targets like the surfaces and atmospheres of planets and their moons. (frontiersin.org)
The fraction of sputtered material that is ionized is extracted into a double-focusing mass spectrometer, which uses electrostatic and magnetic fields to separate the ions according to their mass-to-charge ratio. (nrel.gov)

Standards for quantitative proteomics data formats are still in the early adoption phase and formats like MzQuantML [ 7 ] are not yet widely supported. (biorxiv.org)

Many of the published analyses of quantitative proteomics datasets use complete software packages like Progenesis (Nonlinear Dynamics), PEAKS [ 1 ] or MaxQuant [ 2 ] to name few. (biorxiv.org)
Quantitative analyses can be obtained for most elements in the mass range 5-250 amu (practically 7 Li to 238 U). (lu.se)

and there is significant demand for mass spectrometry (MS) based proteomics tools that can be used to rapidly quantify large numbers of proteins using simple sample preparation. (genomebc.ca)
While quantitative multiple reaction monitoring mass spectrometry (MRM-MS) assays have already been established to measure proteins in human fluids, similar assays have yet to be developed in mice, despite their key role in drug development and biomedical research. (genomebc.ca)
Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)

It is a regulatory requirement to identify and monitor process-related impurities such as HCPs, and a qualitative and quantitative assessment of the components in the final drug product must be performed. (bioprocessintl.com)

and full scan confirmation and multiple reaction monitoring (MRM) for quantitative results. (prweb.com)

Mass spectrometry has transitioned from being used mostly in routine analytical applications to becoming a powerful biological method for protein quantitation. (bio-itworld.com)
We describe the design and synthesis of a novel set of iso-baric tags for quantitative glycan profiling, which will have broad applications in carbohydrate based biomarker dis-covery, therapeutic protein characterization, and vaccine development. (nist.gov)
We demonstrate how GPS can generalize to new data, increase protein identification rates, and increase the overall quantitative accuracy. (lu.se)

Thermo Fisher Scientific is sponsoring a new, free educational webinar , "Novel Psychoactive Substances: challenges and choices in forensic toxicology," which will discuss a number of new designer drugs, analytical approaches, and a range of mass spectral techniques that can be used to definitively identify these substances in a forensic toxicology laboratory. (prweb.com)

This webinar will be of particular interest to researchers, directors, and forensic toxicologists in the life sciences/forensic institutes/health care/doping labs, and core facility personnel who are interested in learning the power of different mass spectrometry techniques in forensic labs. (prweb.com)

MassIVE.quant: a community resource of quantitative mass spectrometry-based proteomics datasets. (cdc.gov)

Modern flight-capable mass spectrometers, in combination with various sample processing, separation, and ionization techniques enable sensitive detection of chemical biosignatures. (frontiersin.org)
High mass resolution (M/ΔM of up to 6000) allows the separation of nominally identical masses (e.g., 31 P from 30 SiH). (nrel.gov)

The fragmentation step makes it possible to identify and separate ions that have very similar m/z-ratios in regular mass spectrometers. (wikipedia.org)
Triple quadrupole mass spectrometers use the first and third quadrupoles as mass filters. (wikipedia.org)

Here, we delve into the search for extraterrestrial chemical and morphological biosignatures and examine several possible approaches to agnostic life detection using mass spectrometry. (frontiersin.org)

Similar considerations as for AMS evolve for the young but growing technique of highly selective laser based mass spectrometry, which utilizes the isobaric and isotopic selective properties of resonant step-wise optical ionization processes. (psi.ch)

This is a method of mass spectrometry that ion fragmentation (m/z) ratio determined through a time of flight measurement. (wikipedia.org)

To bypass these technological hurdles and advance further, and even to provide quantitative capabilities, the most attractive features of affinity proteomics and MS could be combined (17, 18). (academiaperuanadepsicologia.org)

Sequential window acquisition of all theoretical fragment ion spectra (SWATH) is a label-free mass spectrometry quantitative technique, which can retain complete fragment ion data and has good accuracy as well as reproducibility. (ncl.edu.tw)
Interpretation of mass spectra and data treatment will also be covered, together with practical aspects of mass spectrometry method development. (lu.se)

Routine work includes determination of molecular formula and accurate mass with an accuracy of ± 1 mDa of compounds with m/z up to 3000 mDa. (lu.se)

The emphasis is on understanding the fundamental processes involved, the instrumentation used, including different ionization sources and mass analyzers, and interpretation of generated data. (lu.se)

Data independent acquisition mass spectrometry (DIA-MS) has recently emerged as an important method for the identification of blood-based biomarkers. (lu.se)

Each instrumental configuration utilizes a unique mode of mass identification. (wikipedia.org)

Different types of mass analysers will also be discussed, including the quadrupole, the 3d and linear ion traps, the time of flight, the orbitrap and the Fourier transform ion cyclotron resonance mass analysers, and their combinations. (lu.se)

The results uncovered unexpectedly poor nutritional conditions in the actual tumor microenvironment and showed that capillary electrophoresis coupled to mass spectrometry-based metabolomics, which is capable of quantifying the levels of energy metabolites in tissues, could be a powerful tool for the development of novel anticancer agents that target cancer-specific metabolism. (elsevierpure.com)

A novel strategy for quantitative profiling of serum or plasma proteomes is described. (nist.gov)

The quantitative data corroborated well with the corresponding data generated after conventional strong cation exchange fractionation of the same samples. (academiaperuanadepsicologia.org)
In this study, we strived to elucidate how the intrinsic pharmacokinetic (PK)/pharmacodynamic (PD) relationship for CSF Aβ lowering is related to that for brain Aβ through quantitative modeling of preclinical data for numerous BACEi, GSI, and GSM across multiple species. (aspetjournals.org)

Ions of different mass-to-charge ratios are selected by adjusting the strength of the magnetic field. (nrel.gov)

For a precursor ion scan, the product ion is selected in the second mass analyzer, and the precursor masses are scanned in the first mass analyzer. (wikipedia.org)
In a product ion scan, a precursor ion is selected in the first stage, allowed to fragment and then all resultant masses are scanned in the second mass analyzer and detected in the detector that is positioned after the second mass analyzer. (wikipedia.org)

When using multiple quadrupoles, they can act as both mass analyzers and collision chambers. (wikipedia.org)

Anatomy5

Organisms1

Chemicals and Drugs18

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Phenomena and Processes17

Disciplines and Occupations2

Information Science4

Health Care5

Time-of-flight ma2

Tandem16

Chromatography8

Peptides2

Electrospray2

Proteome2

Quantification2

Analysis17

Spectrometer9

Proteomics data1

Analyses2

Proteins3

Qualitative1

Reaction1

Protein3

Substances1

Webinar1

Datasets1

Separation2

Spectrometers2

Detection1

Isobaric1

Measurement1

Affinity1

Spectra2

Determination1

Instrumentation1

Method1

Utilizes1

Fourier1

Metabolism1

Plasma1

Data2

Ions1

Precursor2

Multiple1