Hybridization histochemistrySituImmuno-histochemistryCytochemistryAutoradiographyImmunohistochemistrySemi-quantitativePolymeraseInclude quantitativeMolecularRetrospectiveCollagenTissuesAnalysisDetectionLiverStudiesDataSitu hybridization histochemistryEnzymeBiotechnicImmunohistochemistryQualitativeMorphologicalHistologicalDeterminationMethodsFluorescentAssayImmuneTechniqueEvaluationExpressionStudyScienceProcedures
Hybridization histochemistry12
- We examined the osmotic regulation of ER-β mRNA expression in MNCs using quantitative in situ hybridization histochemistry. (jneurosci.org)
- The present study examined the distribution pattern of C7orf24 mRNA in rat tissues using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization histochemistry. (elsevierpure.com)
- In situ hybridization histochemistry localized C7orf24mRNA to hepatocytes in the liver and renal tubules in the kidney. (elsevierpure.com)
- Most in situ hybridization histochemistry studies of messenger RNA in human brain have been carried out on frozen tissue. (ox.ac.uk)
- We have investigated whether autoclaving also permits in situ hybridization histochemistry to be used quantitatively. (ox.ac.uk)
- We conclude that autoclaving renders formalin-fixed, paraffin wax-embedded sections of human brain suitable for quantitative in situ hybridization histochemistry. (ox.ac.uk)
- We have studied synaptophysin messenger RNA in the neocortex, hippocampus and cerebellum using in situ hybridization histochemistry to see if the encoding transcript can be detected in post mortem human brain and to investigate factors which might influence its abundance. (ox.ac.uk)
- In situ hybridization histochemistry indicated that these increases in gadd45 mRNA occurred in pyramidal neurons located on the edge of the infarcted cortex. (ox.ac.uk)
- The CNS nicotinic cholinergic system is studied using techniques that include quantitative receptor autoradiography, quantitative in situ hybridization histochemistry, ion flux assays and behavioral analyses. (uky.edu)
- The alternative forms of the cannabinoid receptor mRNAs will be localized in rat brain using in situ hybridization histochemistry (ISHH). (grantome.com)
- We investigated the effect of electroconvulsive shock (ECS), administered five times over 10 days, on 5-HT1A and 5-HT2A receptor mRNA and binding site densities in the rat brain using in situ hybridization histochemistry and quantitative autoradiography. (ox.ac.uk)
- Simultaneous Visualization of Vasopressin and Oxytocin mRNA- Containing Neurons in the Hypothalamus Using Non-Radioactive in situ Hybridization Histochemistry. (neurotree.org)
Situ1
- Synaptophysin gene expression in human brain: a quantitative in situ hybridization and immunocytochemical study. (ox.ac.uk)
Immuno-histochemistry2
- ANP, BNP), Western blot protein analysis, in particular post-translational modifications (e.g. phospho-Akt), histochemistry to determine cardiomyocyte size, capillary density and fibrosis, and immuno-histochemistry. (gcure.nl)
- This facility serves as the resource to instruments and equipment needed to perform advanced molecular biology, semi-quantitative immuno-histochemistry and bio-analytical experiments, and support training of junior faculty, postdoctoral fellows, graduate and medical students in the areas of physiology, hypertension and cardiovascular and renal diseases. (tulane.edu)
Cytochemistry2
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. (brain-knowledge-engine.org)
- Journal of Histochemistry and Cytochemistry , 57 (12), 1121-1126. (elsevierpure.com)
Autoradiography1
- Use of storage phosphor screen imaging will allow greatly increased speed of pharmacological screening procedures that utilize quantitative autoradiography. (elsevierpure.com)
Immunohistochemistry2
- Fixed-frozen retinas from adult C57BL/6N mice were used for immunohistochemistry, laser scanning confocal microscopy, and enzymatic histochemistry. (molvis.org)
- Knee joints were collected for histochemistry, immunohistochemistry, and quantitative polymerase chain reaction (qPCR) assays. (edu.au)
Semi-quantitative1
- By 6 h, gadd45 mRNA was shown to increase by semi-quantitative reverse transcriptase - polymerase chain reaction. (ox.ac.uk)
Polymerase1
- This evaluation will involve the use of a herpes simplex virus (type 1)-derived infection and expression system, applying double-labelled ISHH techniques on cultured neurons, and estimating copy number of fusion genes in infected host cells using quantitative polymerase chain reactions (PCR). (grantome.com)
Include quantitative1
- These include quantitative real-time PCR (e.g. (gcure.nl)
Molecular1
- Its quantitative detection has become established as a molecular marker of synaptic density. (ox.ac.uk)
Retrospective2
- In this review, we discuss our previous quantitative results of a retrospective study from 2016 and compare them with the results of older published studies to examine whether GVD is an essential feature of AD dementia or additional neurodegenerative features. (nih.gov)
- A quantitative, descriptive, cross-sectional and retrospective research was performed. (bvsalud.org)
Collagen2
- They also calculated the contraction rate of fibroblast-populated collagen lattice (FPCL) and conducted a reverse transcription-quantitative real time PCR, enzyme-linked immunosorbent assay, lectin histochemistry assay, SDS-PAGE and lectin blotting, western blot assay, and a statistical analysis. (dermatologytimes.com)
- Others samples were stained by Picrosirius red F3BA in order to performed a quantitative analysis of macrophages and collagen. (unifesp.br)
Tissues1
- Quantitative RT-PCR analysis revealed significantly high levels of both C7orf24 mRNAs in the liver and kidney, compared with other tissues examined. (elsevierpure.com)
Analysis3
- Vertical distribution of elements in cells and matrix of epiphyseal growth plate cartilage determined by quantitative electron probe analysis. (brain-knowledge-engine.org)
- On postoperative day 7th or14th, 12 animals of each group were submitted to euthanasia and anterior abdominal wall was removed (without skin) for histologic and immune- histochemistry analysis. (unifesp.br)
- All these disciplines provide qualitative and quantitative analysis of biological fluids such as blood, serum or plasma, tissue, urine, stool, CSF etc. for specific constituents to support Clinicians in the practice of medicine. (hmhealthservices.in)
Detection1
Liver1
Studies6
- However, these quantitative studies have not demonstrated whether GVD is an essential component of AD or non-AD dementias. (nih.gov)
- We invite applications from highly motivated candidates for a Quantitative Biologist/Bioinformatics position working within the Genomics group to design impactful studies and analyze single cell RNA-seq data across many therapeutic areas. (rna-seqblog.com)
- 8. Hellerstr m C, T ljedal I-B & Hellman B: Quantitative studies on isolated pancreatic islets of mammals. (taljedal.se)
- 9. Hellerstr m C, Hellman B & T ljedal I-B: Quantitative studies on phosphatases in the pancreatic islet -cells. (taljedal.se)
- 17. T ljedal I-B: Quantitative studies on isolated pancreatic islets of mammals. (taljedal.se)
- 18. T ljedal I-B: Quantitative studies on phosphatases in isolated pancreatic islets of mammals. (taljedal.se)
Data1
- The successful candidate will engage with the rich quantitative and computational biology community at BMS Cambridge to foster bioinformatic growth through the development of new statistical models of high-dimensional data and implementation of computational pipelines. (rna-seqblog.com)
Situ hybridization histochemistry4
- In situ hybridization histochemistry and autoradiography were used to examine the cellular distribution of mGluR1 receptor subunit mRNA in the Crus II region and quantitative analysis of mGluR1 expression was obtained by measuring silver grain density in 120 Purkinje cell somata for each case from n=9 control and autism cases (Crus II) and n=8 control and autism cases (vermis). (confex.com)
- Most in situ hybridization histochemistry studies of messenger RNA in human brain have been carried out on frozen tissue. (ox.ac.uk)
- We have investigated whether autoclaving also permits in situ hybridization histochemistry to be used quantitatively. (ox.ac.uk)
- We conclude that autoclaving renders formalin-fixed, paraffin wax-embedded sections of human brain suitable for quantitative in situ hybridization histochemistry. (ox.ac.uk)
Enzyme4
- Enzyme histochemistry for myophosphorylase is easy to perform and when absent is diagnostic for McArdle disease. (medscape.com)
- Enzyme histochemistry of 19-year-old male with McArdle disease. (medscape.com)
- Fraction of the SC and LGB meant for quantitative enzyme histochemistry were homogenized in sucrose solution and processed accordingly for the activities of lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G6PD) and acetylcholinesterase (AChE) while the fraction of the SC and LGB meant for histological and immunohistochemical analysis were fixed in 10% formol-calcium solution and were processed for paraffin embedding, respectively. (academicjournals.org)
- 8. Qualitative and quantitative enzyme histochemistry of the human endometrium and cervix in normal and pathological conditions. (nih.gov)
Biotechnic1
Immunohistochemistry2
- Adipose tissue deposits were excised, weighed and evaluated by a set of biochemical, histological and molecular procedures, such as: thin-layer chromatography, histochemistry, immunohistochemistry, quantitative real-time polymerase chain reaction, high-resolution oxygraphy, ATP synthesis and enzymatic activity measurements. (bioblast.at)
- Immunohistochemistry ( ihc ), also known as immunocytochemistry , refers to a new technology that the qualitative, localized, and quantitative determination of the corresponding antigens is performed through antigen-antibody reaction and histochemical color reaction in tissue cells' situ by specific antibodies labeled with a chromogenic reagent. (ballyabio.com)
Qualitative1
- Because the complex of antigen and antibody is colorless, it is necessary to show the binding site of antigen and antibody by means of histochemistry to achieve qualitative, localized or quantitative research on unknown antigens in tissues or cells. (ballyabio.com)
Morphological2
- S. Al-Hussain, R. Al-Saffar and S. Zaqout, "Morphological and Quantitative Study of Neurons in the Gracile Nucleus of the Camel Brain Stem," Journal of Behavioral and Brain Science , Vol. 2 No. 1, 2012, pp. 35-47. (scirp.org)
- Quantitative morphological research bowel had been completed by design-based stereology, directing on epithelium, lamina propria, clean muscles, eosinophils and also CD3(+) cellular material. (hormonessignaling.com)
Histological2
- In this article, Anfinsen, Lowry, and Hastings presented a method that permitted histological examination and quantitative enzymatic study of individual freeze-dried sections of tissue in order to study enzymatic localization in the various layers of the retina. (nih.gov)
- Histological studies have been performed right after H&E-, PAS- and also Congo red-staining, whilst immune histochemistry ended for diagnosis associated with CD3(+) cells. (hormonessignaling.com)
Determination2
Methods2
- In addition, iron levels measured by LA-ICP-MS and three quantitative MRI methods, namely R2∗ (=1/T2∗), image phase and quantitative susceptibility mapping (QSM), were compared between 19 AD and 11 controls. (archive.org)
- In conclusion, our results show that histochemistry as well as quantitative MRI methods such as R2∗ mapping and QSM provide reliable measures of iron distribution in the cortex. (archive.org)
Fluorescent1
- Albanese A, Bentivoglio M. Retrograde fluorescent neuronal tracing combined with acetylcholinesterase histochemistry . (albanese.biz)
Assay1
- Moreover, quantitative PCR (qPCR), western blot, CCK8 assay, transwell assay, and wound-healing assay were used to detect the expression level and function of YTHDF2 under hypoxia exposure in LUSC cells. (biomedcentral.com)
Immune1
- It cleverly combines the specificity of the immune response and the visibility of histochemistry. (ballyabio.com)
Technique2
- Whole-body insulin sensitivity (by the insulin clamp technique), intramyocellular lipids (by quantitative histochemistry on quadriceps muscle biopsies), muscle insulin action (as the expression of Glut4 glucose transporters), and postprandial lipemia were measured in 20 morbidly obese patients (BMI = 49 ± 8 [mean ± SD] kg · m -2 ) and 7 nonobese control subjects. (medscape.com)
- Published shortly before Anfinsen received his Ph.D. from Harvard in 1943, this is a quantitative, histochemical study of the retina in which the three researchers adapted an earlier technique for the identification of chemical components in cells and tissues that was developed by Kaj Linderstrom-Lang and K.R. Morgan. (nih.gov)
Evaluation2
- P. E. Bermejo, C. E. Jimenez, C. V. Torres and C. Avendano, "Quantitative Stereological Evaluation of the Gracile and Cuneate Nuclei and Their Projection Neurons in the Rat," Journal of Comparative Neurology, Vol. 463, No. 4, 2003, pp. 419-433. (scirp.org)
- MT2 appeared as the most valuable immunophenotypic marker as emerged from a comprehensive quantitative evaluation of 2329 reactive follicles and 2288 neoplastic follicles performed on MT2 immunostained sections. (univr.it)
Expression1
- His tutorial and code on "Quantitative High-Throughput Gene Expression Imaging" gives a great introduction to how one might solve a real-world problem once, then leverage that work to batch process the next hundred (or thousand, or hundred thousand) images. (mathworks.com)
Study1
- The purpose of the current study was to compare quantitative measures of vessel number, radius, and tortuosity as defined from MRA images of healthy volunteers 60-74 years of age, each of whom self-reported aerobic exercise activity levels as high or low. (ajnr.org)
Science1
- Analytical science includes the subjective and quantitative assurance of the compound parts of substances. (neurotray.com)
Procedures1
- Logical procedures incorporate both subjective and quantitative investigation. (neurotray.com)