Quantitative biological assay. Medical search. Frequent questions

If you are interested in a fluorometric detection kit, please check Nitric Oxide Assay Kit (Fluorometric) (ab65327) . (abcam.com)
Quantitative detection of α-Synuclein and Tau oligomers and other aggregates by digital single particle counting. (fz-juelich.de)
Up-converting phosphor technology-based lateral flow assay for quantitative detection of β-hydroxybutyrate in biological samples. (moleculardepot.com)
Linear detection range is 0.66-500 U/L glutaminase in a 96-well plate assay. (bioassaysys.com)
Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. (immpact-international.org)
This methodology enables detection of putative metabolites from biological samples and simultaneous quantification of the pattern and extent of isotope labeling. (acs.org)
I. To prospectively analyze the concordance between detection of MYCN amplification in tumor samples and quantitative detection of MYCN DNA in serum, and to analyze the prognostic significance of MYCN amplification as detected in serum samples. (uchicagomedicine.org)
Linear detection range: 4.8 to 500 µM or 0.054-5.7 mg/dL (colorimetric assay) and 0.25 to 100 µM or 0.0028-1.14 mg/dL (fluorimetric assay) for a 60 min reaction. (biolisp.org)
Noncompetitive immunoassays are advantageous over competitive assays for the detection of small molecular weight compounds. (cdc.gov)
It is designed to calibrate RT-qPCR assays and test the limit of detection for all target genes. (industry.gov.au)
In particular, its observations, conclusions and recommendations provide timely recommendations and reference preparations for assuring the quality, safety and efficacy of vaccines, and the provision of reference materials for standardizing essential diagnostic assays for the detection of virological contaminants in blood products. (who.int)

The development and validation process of a high throughput, high-plex mIF assay to derive meaningful biological insights. (genengnews.com)
Can be readily automated as a high-throughput assay for thousands of samples per day. (immpact-international.org)
These include single-cell and spatial omics, high-throughput drug- and CRISPR-based perturbation assays, and quantitative imaging. (embl.org)

Though many traditional sensing technologies for biomarker analysis and fundamental biological studies (i.e. enzyme-linked immunosorbent assays, real-time polymerase chain reaction, etc.) rely on single-parameter measurements, it has become increasingly clear that the inherent complexity of many human illnesses and pathways necessitates quantitative and multiparameter analysis of biological samples. (illinois.edu)
Here, we investigate the association of leukocyte telomere length with schizophrenia with the quantitative polymerase chain reaction method in independent Japanese cohorts consisting of 1,241 patients with schizophrenia and 1,042 controls, which we believe is the largest ever as an independent sample set of telomere studies in schizophrenia. (databasefootball.com)
The behavior of mesenchymal stem cells from an umbilical cord vein was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a mineralization assay, and an alkaline phosphatase activity assay and by carrying out a quantitative real-time polymerase chain reaction for osteogenic markers. (biomedcentral.com)
Major candidates were then re-evaluated by absolute quantitative polymerase chain reaction analysis in an independent cohort of patients with PTC (n = 44) or benign nodules and 20 healthy controls (HCs). (medscape.com)

This product is manufactured by BioVision, an Abcam company and was previously called K262 Nitric Oxide Colorimetric Assay Kit. (abcam.com)

BioAssay Systems' DGLN-100 Kit provides a convenient fluorimetric method to measure glutaminase activity in biological samples. (bioassaysys.com)
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you. (bioassaysys.com)

While the key specificity of this assay is based on the aptamer's inherent affinity to membrane proteins on exosomes, exosomes' inherent huge mass/volume acts as mass-based fluorescence polarization amplifier. (rsc.org)
Therefore, many presumptive positive specimens require confirmation by a higher specificity second-tier assay employing separations, which require additional time and resources prior to patient follow-up. (cdc.gov)
The CE-HRMS platform resolved problematic isobars and isomers that affect first-tier FIA-MS/MS assay specificity, while achieving similar quantitative results and assay linearity. (cdc.gov)

While quantitative multiple reaction monitoring mass spectrometry (MRM-MS) assays have already been established to measure proteins in human fluids, similar assays have yet to be developed in mice, despite their key role in drug development and biomedical research. (genomebc.ca)
The assay which was developed and validated was performed by flow injection analysis-tandem mass spectrometry (FIA-MS/MS). RESULTS: Interferents of tHcy measurement were identified, so selective derivatization of Hcy was employed. (cdc.gov)
BACKGROUND: Most first-tier newborn screening (NBS) biomarkers are evaluated by a 2-min flow injection analysis coupled to tandem mass spectrometry (FIA-MS/MS) assay. (cdc.gov)

The Expert Committee on Biological Standardization meets annually to establish detailed recommendations and guidelines for manufacturing, licensing, and control of complex biological materials, including blood products, vaccines, and related in vitro diagnostic tests, and to maintain a catalog of IRPs to be distributed globally as required. (cdc.gov)
The Expert Committee on Biological Standardization reviews developments in the field of biological substances used in human medicine, which include vaccines, biological therapeutics, blood products and related in vitro diagnostic devices. (who.int)
Comparability of data worldwide is assured by the use of international reference materials in designating the activity of prophylactic or therapeutic biological substances or in ensuring the reliability of quality control or diagnostic procedures. (who.int)
BACKGROUND: Irradiative sterilization of clinical specimens prior to chemical laboratory testing provides a way to not only sterilize pathogens and ensure laboratorian safety but also preserve sample volume and maintain compatibility with quantitative chemical diagnostic protocols. (cdc.gov)

ExoTEST consists of ELISA plates pre-coated with patented panexosome antibodies that allow specific capture of exosomes from a variety of biological samples, including cell culture supernatants and human biological fluids. (immpact-international.org)
This dissertation describes the fabrication, characterization, development, and application of silicon photonic microring resonator technology to multiplexed protein measurements in a variety of biological systems. (illinois.edu)
The assays target proteins with a wide variety of biological functions, including proteins putatively involved in cardiovascular disease and cancer, making them important for future protein biomarker discovery and verification studies. (genomebc.ca)

Cuvette or 96-well plate assay formats possible. (immpact-international.org)

In particular ExoTEST is a successful platform for exosomes quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. (immpact-international.org)
Here we have developed an aptamer-based fluorescence polarization assay for exosome quantification, which is a separation-free, amplification-free and sensitive approach enabling direct quantification of exosomes in human plasma. (rsc.org)

The quantitative assessment of the accuracy and precision of such multiplex panels however is key for their widespread use in clinical samples. (genengnews.com)

Early stage mechanism-based in vitro assays can not only speed up the compound development pipeline, but also can reduce animal testing requirements. (labroots.com)

Since 1947, the provision of WHO reference materials has played a vital role in the translation of laboratory science into worldwide clinical practice and has been delivered through the WHO Expert Committee on Biological Standardization, WHO collaborating centers, and various state and nonstate partners. (cdc.gov)

The tissue-specific expression patterns of these RNA species and their high stability in biological fluids are well established. (medscape.com)

The Assay Creatineng Uk reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (biolisp.org)
Serum interleukin (IL)-1, IL-6, tumour necrosis factor (TNF) and quantitative urinary -2-microglobulin (-2-m) excretion were estimated. (who.int)
Trends toward more conservative management of papillary thyroid cancer (PTC) diminish the primacy of serum thyroglobulin (Tg) assays as a posttreatment surveillance tool. (medscape.com)
miR-146a-5p and miR-221-3p hold remarkable promise as serum biomarkers for post-treatment monitoring of PTC patients, especially when Tg assay results are uninformative. (medscape.com)

The SuFIDA Innovation Lab develops digital assays for various biomarkers with highly specific single-molecule sensitivity. (fz-juelich.de)

Immunoassays such as ELISAs or molecular methods such as quantitative PCR can be used to quantify biological materials, but these assays are subject to inherent variability between test instances and different laboratories, and require the use of reference preparations to generate a quantitative output ( 2 ). (cdc.gov)

Accurate methods and extensive simulations promise quantitative comparison with experiments across biochemistry. (manchester.ac.uk)
Currently used analytical methods are deficient in that they often provide either highly quantitative data for a single biomarker or qualitative data for many targets, but methods that simultaneously provide highly quantitative analysis of many targets have yet to be adequately developed. (illinois.edu)
The Huber group develops statistical methods for modern biotechnologies, applies them to biological discovery, and translates them into reusable tools. (embl.org)
Our team studies biological systems by developing statistical and computational methods for the analysis of new data types and novel, large systematic datasets. (embl.org)
This inconsistency may be partially due to the relatively small sample sizes in each study and heterogeneity caused by various uncontrolled confounders (e.g., duration of illness or hospitalization, lifetime antipsychotic dose, and telomere length assay methods). (databasefootball.com)
In particular, statistical and other quantitative methods research by ICB highlighted the fact that to estimate infection-associated cancers. (who.int)
It provides an ensemble of high quality solutions, which are analyzed with clustering methods and display a hierarchical structure highlighting distinct parameter subspaces with biological interpretations. (lu.se)
In unsupervised methods, providing explanatory insights into addition, we predict the response of the system when varying the data and related biological interpretations. (lu.se)

I want to get the latest chemistry news from C&EN in my inbox every week. (acs.org)
Biological chemistry. (fz-juelich.de)

The method involves simple sample preparation, is compatible with low sample volumes, and involves assay validation according to high-standard guidelines created by the proteomics community. (genomebc.ca)
Recent advances in peptide-based (bottom-up) quantitative proteomics and bioinformatics have opened unprecedented opportunities for extensive investigation of cellular proteomes and their dynamics. (acs.org)

For quantitative determination of glutaminase activity in biological samples. (bioassaysys.com)
Description: For quantitative determination of zinc ion Zn 2+ and evaluation of drug effects on zinc metabolism. (immpact-international.org)
Description: For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation. (immpact-international.org)
These assays are performed on a Behring Nephelometer for quantitative CRP determination. (cdc.gov)

For quantitative analytical procedures, accuracy can only be assessed if a true or accepted reference value is available. (gmp-compliance.org)

The history of protein dynamics traces back to 1935, when Schoenheimer and Rittenberg synthesized the first isotopologs of biological molecules to demonstrate the continuous renewal of proteins throughout life 14 , 15 . (nature.com)
Integrated isolation and quantitative analysis of exosome-transmitted proteins and nucleic acids using immunocapsura approaches. (immpact-international.org)
The mystery and the potential biological significance of these unknown genes is enhanced by many of them being well conserved, and many of them being completely unrelated to known proteins and thus lacking clues to their function. (biorxiv.org)
http://toxsci.oxfordjournals.org/ node assay (LLNA) is viewed as the most appropriate skin react with proteins for the effective induction of skin sensitization. (cdc.gov)

These WHO IRPs, composed of international reference standards and other reference materials ( Table 1 ), provide a common set of reagents that are used to ensure the quality of biological assays and medicines globally. (cdc.gov)
Creatinine Assay Laboratories manufactures the assay creatineng uk reagents distributed by Genprice. (biolisp.org)

SuFIDA (surfaced-based fluorescence immuno digital assay) is the combination of the proven principle of a sandwich ELISA and digital readout using highly sensitive fluorescence microscopy. (fz-juelich.de)
ExoTEST is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. (immpact-international.org)
The assay setup with the biotinylated antibody immobilized onto streptavidin-coated magnetic beads significantly reduced the amount of coating antibody giving identical sensitivity (50% saturation of the signal (SC(50))=0.2-0.4ng/ml) to the best result obtained with direct coating of the antibody on ELISA plates. (cdc.gov)

These biological mediators can include cytokines, proteases, lipid metabolites and more. (case.edu)
Although the Core focused on quantitative analysis, the overall services include gene expression, transcriptional activation, secreted biological mediators and functional assays. (case.edu)

The Bioanalyte Core utilizes a variety of technologies both singular and multiplexing to facilitate the quantitative and qualitative analysis of bioanalytes. (case.edu)

In this assay, o-phthalaldehyde reacts with liberated ammonia where the increase in fluorescence at λex/em = 415/475 nm is directly proportional to enzyme activity. (bioassaysys.com)

Simulations can now be used as computational assays of biological activity, for example, in predictions of drug resistance. (manchester.ac.uk)
We describe a computational methodology allowing to systematically and quantitatively characterize a Boolean mathematical model of a biological network in terms of genetic interactions between all loss of function and gain of function mutations with respect to all model phenotypes or outputs. (researchgate.net)

the understanding of MSC biology, their mechanism of action (MoA) in different biological contexts, and their targeted and routine use in the clinics is limited ( Hoogduijn and Lombardo, 2019 ). (frontiersin.org)
Experimentally characterized genetic interaction networks in model organisms provide important insights into relationships between different biological functions. (researchgate.net)
This study design may have overcome some potential biases that could not be controlled in previous studies (e.g., small sample size, varying LTL assay protocols, different biological bases in young, middle-aged, and elderly patients with schizophrenia, the effect of duration of illness or hospitalization, and antipsychotic dose). (databasefootball.com)
This analysis discriminates between different biological mechanisms to achieve a transient signal from a sustained TGF-b input, where one mechanism is to use a negative feedback to turn the signal off. (lu.se)
responsible for many different biological functions, in- 1. (lu.se)

Fluorimetric assay: 0.25 µM or 0.0028 mg/dL. (biolisp.org)

Biological Assay" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ctsicn.org)

Creatinine Assay Kit measures creatinine levels in urine. (biolisp.org)
The bead-based assay tolerated up to 10 and 5% of methanol and urine matrix, respectively. (cdc.gov)

Quantitative activation suppression assay to evaluate human bone marrow-derived mesenchymal stromal cell potency. (ctsicn.org)

In its current stage, these words are not written with the intent to be precise or complete, but to guide my own thoughts in the understanding - however superficial - of which are the general principles, opposite to specific molecular mechanisms, that drive biological processes and that are more likely to help us in the understanding of human physiology and pathological states. (quantitative-biology.org)
Their scope crosses the broad spectrum of candidate vaccines, from those prepared by the classical method of virus attenuation by application of biological selection pressures to those based on the latest molecular biological approaches to the design of novel virus constructs. (who.int)

The total assay time is about 30 min with just one mix-and-read step to achieve high sensitivity. (rsc.org)

Under most conditions however, both social and experimental, considerably less of the THC actually enters the physiological system[n] The lack of standard administration techniques generally renders detailed or quantitative comparisons quite tenuous, both among experiments and between laboratory and social situations. (druglibrary.net)
The Core can also develop assays which are not currently commercially available, and run clinical samples through certification by the American Board of Medical Laboratory Immunology to fulfill CLIA based applications. (case.edu)
in addiTion, icB offerS many laBoraTory aSSayS ThaT are widely uSed in epidemiological reSearch. (who.int)

Analysis of anticoagulants for blood-based quantitation of amyloid beta oligomers in the sFIDA assay. (fz-juelich.de)
cytokine quantitation is thus important in fundamental biological studies and diagnostics, and complex and overlapping cytokine roles make multiplexed measurements especially vital. (illinois.edu)

Our assay allows quantitative analysis of exosomes in the range of 5 × 10 2 to 5 × 10 5 particles per μL with a detect limitation of 500 particles per μL for the cell line deprived exosomes. (rsc.org)
This work describes a new and simple liquid biopsy assay to directly detect exosomes in the biological matrix, which facilitates cancer diagnosis and therapy monitoring. (rsc.org)

This work ensures assay robustness and aids uptake into other laboratories. (genomebc.ca)

The developed assays have been translated into easy-to-use kits, which include all necessary protocols and instrument settings. (genomebc.ca)
Indeed, only one study has recruited more than 1,000 patients with schizophrenia (and a sufficient sample of controls), although this was a meta-analysis, including different telomere length assay protocols. (databasefootball.com)

Particle-enhanced assays are based on the reaction between a soluble analyte and the corresponding antigen or antibody bound to polystyrene particles. (cdc.gov)

icB iS mainly focuSed on The characTerizaTion of The Biological properTieS of well-eSTaBliShed and novel poTenTial oncogenic viruSeS. (who.int)
ICB's findings showed that assays established by the Group, aiming has coordinated the HPV-AHEAD several oncogenic viruses have the to characterize the natural history of consortium, which included a multi- ability to induce epigenetic changes and several viruses at different anatomical disciplinary team in Europe and deregulate cel ular gene expression. (who.int)

Can be readily automated to assay thousands of samples per day. (bioassaysys.com)

The unique combination of biological and structural studies at the Department of Immunology & Theranostics, has created a thriving, productive environment and a fruitful collaboration among investigators at City of Hope and at other institutions. (cityofhope.org)

We conclude that further development of targeted approaches should prevent inaccurate conclusions about the nature of biological regulations and in particular kinase-substrate networks. (acs.org)
CONCLUSIONS: The results from this pilot study support the implementation of an irradiative sterilization protocol for possible mixed-exposure samples containing both chemical and biological threat agents (mixed CBTs). (cdc.gov)

Low interference in biological samples. (immpact-international.org)
This protocol outlines a method for quantitative analysis of mitophagy protein complex formation specifically in beta cells from primary human islet samples. (jove.com)
This technique thus allows analysis of mitophagy from limited biological material, which are crucial in precious human pancreatic beta cell samples. (jove.com)
We study fundamental biological model systems, as well as clinical samples for direct applications in biomedicine and precision oncology. (embl.org)

The Expert Committee also adopted new guidelines for the production and quality control of candidate live attenuated tetravalent dengue vaccines, and revised recommendations for the preparation, characterization and establishment of international and other biological reference standards. (who.int)
The revised recommendations for the preparation, characterization and establishment of international and other biological reference standards set out the process whereby such reference preparations are established and the technical specifications to which they must comply. (who.int)
The scientific basis for characterization of biological reference materials was reviewed in a series of consultations with the scientific community, national regulatory authorities, international standards setting bodies and users. (who.int)

Biomolecular simulation is increasingly central to understanding and designing biological molecules and their interactions. (manchester.ac.uk)

Fields such as medical diagnostics and cellular biology would benefit greatly from a technology that enables rapid, quantitative and reproducible assays for many targets within a single sample. (illinois.edu)
Our interdisciplinary team comprises researchers from quantitative disciplines - such as physics, mathematics, statistics, computer science - and different fields of biology and medicine. (embl.org)

For an assay kit to detect nitrite alone see Griess Reagent Kit ab234044 . (abcam.com)

In the first case study, we observed that the real biological networks were most sensitive to overexpression/state-flip and edge-addition/-reverse mutations among node-based and edgetic mutations, respectively. (researchgate.net)

Such an assay would reduce the volume of false positives and inconclusive specimens flagged for second-tier screening. (cdc.gov)

The degree has an engaging and dynamic curriculum that deals with the underpinning science of the drug development process, including physical, chemical and biological concepts. (qub.ac.uk)
Projects range from applied data analysis for biological discovery to theoretical method development. (embl.org)

Procedure: Assay takes 30 min. (immpact-international.org)

Complex macromolecules, such as immunoglobulins, or genetic material often cannot be adequately characterized by chemical or physical means alone, and reference preparations are necessary to enable consistency and comparison across assays. (cdc.gov)

The Expert Committee provides up-to-date recommendations on the quality, safety and potency of biological substances used in human medicine and ensures the availability of necessary international reference materials. (who.int)

In this assay, I describe reflections on biological systems and the nature of life. (quantitative-biology.org)

For this purpose, the Innovation Lab develops standardized digital assays using SuFIDA technology and has pipetting robots and automated 'high-end' microscopes. (fz-juelich.de)

Use of IRPs also ensures consistency of production and quality of biological medicinal products and is essential for establishment of appropriate clinical dosing. (cdc.gov)

these biological variables will be analyzed for independent clinical significance compared to MYCN amplification, International Neuroblastoma Staging System (INSS) stage, age, ploidy, and histologic variables in predicting either response to treatment or outcome. (uchicagomedicine.org)

Anatomy3

Organisms2

Diseases3

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes13

Anthropology, Education, Sociology and Social Phenomena1

Information Science5

Detection11

High throughput3

Polymerase chain re4

Colorimetric Assay Kit1

BioAssay Systems2

Specificity3

Mass spectrometry3

Diagnostic4

Variety of biological3

Plate assay1

Quantification2

Multiplex1

Vitro1

Expert Committee on Biological Standardization1

Fluids1

Serum4

Biomarkers1

Immunoassays1

Methods8

Chemistry2

Proteomics2

Determination4

Analytical1

Proteins4

Reagents2

ELISA3

Mediators2

Qualitative1

Fluorescence1

Computational2

Different biological5

0.251

MeSH1

Urine2

Mesenchymal1

Molecular2

Sensitivity1

Laboratory3

Quantitation2

Exosomes2

Laboratories1

Protocols2

Reaction1

Oncogenic2

Readily automated1

Immunology1

Conclusions2

Samples4

Characterization3

Molecules1

Biology2

Detect1

Sensitive1

Specimens1

Development2

Procedure1

Preparations1

Human1

Describe1

Develops1

Ensures1

Clinical1