Quantitative 3 cells harbor. Medical search. Frequent questions

[10] FGF2, in conjunction with BMP4 , promote differentiation of stem cells to mesodermal lineages. (wikidoc.org)
After differentiation, BMP4 and FGF2 treated cells generally produce higher amounts of osteogenic and chondrogenic differentiation than untreated stem cells. (wikidoc.org)
The members of the retinoblastoma family of pocket proteins, pRb, p107 and p130, are negative regulators of the cell cycle with tumor suppressor activity and with roles in differentiation. (oncotarget.com)
Cell death and differentiation. (rochester.edu)
20. Melchers, F.: Biochemical characterization of immunoglobulin-producing B-lymphocytes at different stages of their differentiation.In: 'Regulation of Growth and Differentiated Function in Eukaryotic Cells,' eds. (uni-mainz.de)
The Ras family of small GTPases transmits extracellular signals that regulate cell growth, differentiation, motility and death. (oncotarget.com)
The Ras family of small GTPases transmits signals initiated by cell surface receptors, to regulate several cellular processes such as cell growth, differentiation, motility and death [ 1 ]. (oncotarget.com)
A novel testis-specific metallothionein-like protein, tesmin, is an early marker of male germ cell differentiation. (embl.de)
Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. (cshlpress.com)

His research on mouse erythroleukemia cells led to articles in prestigious scientific journals such as CELL, Developmental Biology, and Cold Spring Harbor Symposia on Quantitative Biology. (factual-space.com)
Methods in Molecular Biology, 1509, pp.93-113.Available at https://link.springer.com/protocol/10.1007/978-1-4939-6524-3_10 [Accessed 25 Feb. 2020]. (bioscience.co.uk)
Open Biology, 3 (SEP). (cshl.edu)
A current paradigm in biology is that basal cells present in the epithelial lining of some organs never come into contact with the inner (luminal) side of the organ. (nih.gov)

Cold Spring Harbor Laboratory. (nih.gov)
Based on a popular course at Cold Spring Harbor Laboratory, this new manual assembles cutting edge protocols, helpful hints, and lecture notes to teach researchers from a wide variety of disciplines the essential methods of proteomics using state of the art instrumentation. (cshlpress.com)
Proteomics: A Cold Spring Harbor Laboratory Course Manual can be used both as the basis for a course and as a detailed bench manual for those performing indispensable proteomic experiments. (cshlpress.com)
Cold Spring Harbor Laboratory is a leading international center for research and education. (cshl.edu)
Cold Spring Harbor Asia Conferences is our sister program based in Suzhou, China. (cshl.edu)
Symposia, conferences and summer schools follow the Cold Spring Harbor tradition in showcasing merit-based scientific advances in an informal but intense atmosphere. (cshl.edu)
Thanks to major federal, foundation and corporate support of our programs, we provided stipends to more than 900 graduate students and postdocs attending meetings and courses at Cold Spring Harbor in 2019. (cshl.edu)
In: 'Control of Proliferation in Animal Cells', Cold Spring Harbor Laboratory, pp. 393-410, 1974. (uni-mainz.de)
Cold Spring Harbor Laboratory Press, 8:181-194. (jpmb-gabit.ir)

In situ hybridization analysis in adultmouse testis showed that tesmin is specifically expressed in spermatocytes.Quantitative RT-PCR at different stages of mouse postnatal development (days 4,8, 12, 18, and 42) revealed that tesmin is expressed as early as day 8 andcoincides with the entry of germ cells into meiosis. (embl.de)
The frequencies of CNVs in these populations were analyzed by oligonucleotide array comparative genome hybridization, quantitative PCR, PCR, DNA sequencing across breakpoints, and single-worm PCR. (biomedcentral.com)
In situ hybridization (ISH) and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to assess the level of PTTG3P and prognostic values of PTTG3P were assayed in two HCC cohorts ( n = 46 and 90). (biomedcentral.com)
The expression and localization of PTTG3P were analyzed by quantitative real time polymerase chain reaction (qRT-PCR) and in situ hybridization (ISH), respectively, using patient samples from 2 HCC cohorts. (biomedcentral.com)

CXPA is a rare and aggressive malignant tumor, whose prevalence represents approximately 3%-6% of all salivary gland tumors. (bvsalud.org)
Alteration or exacerbation of malignant tumor cell migration and dissemination is the principal cause of death due to solid tumors 5 . (nature.com)
Development of most solid tumors is characterized by an increase in secretion and activation of matrix metalloproteinases (MMPs) produced by either the tumor cells or tumor-associated fibroblasts. (bvsalud.org)
Photocarcinogenesis in hairless mice induced tumors harbors more UVA than UVB fingerprint by ultraviolet A tanning devices with or without mutations: A role for UVA in human skin car- subsequent solar-simulated ultraviolet irradia- cinogenesis. (who.int)

To identify novel molecular targets within the ubiquitination pathway that positively regulate migration we conducted a loss-of-function genetic screen using an epithelial cell line derived from human triple-negative breast cancer (MDAMB231) infected with a pooled shRNA interference library. (nature.com)
Over the past decades, luminal epithelial cell lineage has gained considerable attraction as the functionally milk-secreting units and as the most fruitful acreage for breast cancer launching. (biomedcentral.com)
They will take this work one step further and create new model systems to determine the 3-D relationships and functions of different epithelial cell types as the basal cells detect and respond to various drugs, hormones, chemicals and pathogens that appear in the cavity of the organ. (nih.gov)

During the 2002 phocine distemper epidemic, 2,284 ing average daily wind force at Den Helder, North Holland seals, primarily harbor seals ( Phoca vitulina ), were found (obtained from the Royal Netherlands Meteorological stranded along the Dutch coast. (cdc.gov)
Of 1,315 seals that underwent necropsy, com- either immunoglobulin M to morbillivirus by serology, plete data were obtained for 1,096 harbor seals ( Phoca vit- morbillivirus-specific nucleic acid by reverse-transcrip- ulina ) (Table 1). (cdc.gov)
2011. Genetic substructure of harbor seal (Phoca vitulina richardsi) populations along the coasts of Washington and Oregon. (noaa.gov)

Though the lab's primary focus is on statistical/quantitative genetics, we collaborate broadly with experimentalist groups to validate and inform our statistical hypotheses. (dana-farber.org)
3 Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran. (jpmb-gabit.ir)

Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)
It was also shown to act on preosteoblasts - in the form of an increased proliferation - after binding to fibroblast growth factor receptor 1 and activating phosphoinositide 3-kinase . (wikidoc.org)
Deletions of multiple GID subunits compromise cell proliferation, and this defect is accompanied by deregulation of critical cell cycle markers such as the retinoblastoma (Rb) tumor suppressor, phospho-Histone H3 and Cyclin A. We identify the negative regulator of pro-proliferative genes Hbp1 as a bonafide GID/CTLH proteolytic substrate. (elifesciences.org)
Enforced expression of PTTG3P significantly promoted cell proliferation, migration, and invasion in vitro, as well as tumorigenesis and metastasis in vivo. (biomedcentral.com)
Non-Hodgkin Lymphomas Non-Hodgkin lymphomas are a heterogeneous group of disorders involving malignant monoclonal proliferation of lymphoid cells in lymphoreticular sites, including lymph nodes, bone marrow, the. (msdmanuals.com)

3) Inferring complex trait architecture at genome scale. (dana-farber.org)
This topical collection on " Gene Editing " aims to provide a forum for discussions on the latest technical developments in the fields of general genome engineering technologies, including (i) the establishment of cell culture systems, and (ii) the development of established and emerging organismal models by CRISPR/Cas9 or similar genome engineering tools. (mdpi.com)
Recent work has demonstrated that clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems1-3 can serve as the basis of a simple and highly efficient method for performing genome editing in bacteria, yeast and human cells, as well as in vivo in whole organisms such as fruit flies, zebrafish and mice4-13. (cdc.gov)

The discovery of leptin more than a decade ago established a paradigm in which secreted proteins from adipocytes coordinate energy balance and glucose homeostasis ( 2 , 3 ). (diabetesjournals.org)
[3] [5] FGF2 binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins which themselves constitute a family of closely related molecules. (wikidoc.org)
Cell motility is a complex process that requires post-translational regulation of a wide variety of proteins. (nature.com)
This study involves precisely defining by molecular techniques the individual signaling proteins expressed in the acinar cell and then subsequently assessing if individual agonists utilize discrete and different elements of the PI-signaling pathway. (rochester.edu)
Cellular proteostasis involves the coordinated and compensatory action of pathways that control biogenesis, folding, trafficking and breakdown of proteins allowing the cell to adapt to physiological or pathological environmental changes. (elifesciences.org)
The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. (cshlpress.com)
These viral proteins also interact with other several cellular factors and lead to revoke normal cell cycle checkpoint stand cell death mechanisms 13 . (bvsalud.org)

However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
In order to identify novel molecular targets of tumor cell migration and invasion we performed a genetic screen with an shRNA library against ubiquitination pathway-related genes. (nature.com)
Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies. (cshlpress.com)

Our discovery that CD32a+ CD4 T lymphocytes harbor the elusive HIV-1 reservoir is a crucial step towards specific targeting and elimination of this HIV-1 reservoir. (europa.eu)

We extended our investigation in vivo and confirmed that mice injected with USP19 depleted cells display increased tumor-free survival, as well as a delay in the onset of the tumor formation and a significant reduction in the appearance of metastatic foci, indicating that tumor cell invasion and dissemination is impaired. (nature.com)
[3] In female mice, the epigenetic signal is maintained through the F2 generation as a result of the exposure of the germline in the womb. (cloudfront.net)
Furthermore, adult W/Wvsterile mice that harbor the c-kit mutation lacked tesmin expression. (embl.de)

Simple Summary Cutaneous squamous cell carcinoma (cSCC) is the second most common form of skin cancer, which predominantly occurs on the head and neck. (unav.edu)
To determine the expression of tissue inhibitors of metalloproteinases (TIMP-2) in oral squamous cell carcinoma (OSCC) and the difference in its expression level between positive and negative HPV-16 (human papilloma virus- 16) OSCC patients. (bvsalud.org)
The recurrence is a serious prognostic influence in patients with oral squamous cell carcinoma (OSCC) 1-3 . (bvsalud.org)
2014) 16 reported and compared the histologic, immunohistochemical and clinical features as well as the survival rates of carcinoma arising in patients with PVL (p-scca) and conventional squamous cell carcinoma (c-scca). (bvsalud.org)
risk of basal and squamous cell skin cancer. (who.int)

Recently, Dr. Breton and colleagues made a paradigm-shifting discovery that these "so called" basal cells actually extend slender body projections that reach the luminal side of the epithelia and control their function via crosstalk with other epithelial cells. (nih.gov)

Although all Ca2+ mobilizing agonists in pancreatic acinar cells utilize the phosphoinositide-signaling (PI) pathway, stimulation by individual agents results in markedly different temporal and spatial patterns of Ca2+ signaling. (rochester.edu)

DNA is wrapped into a DNA-protein complex called chromatin in the nucleus of eukaryotic cells. (cloudfront.net)
The tso1 mutant phenotypes and the novelty of the TSO1sequence suggest the existence of previously unknown participants in regulationof directional processes in eukaryotic cells. (embl.de)
Although Maraffini and colleagues7 recently performed a systematic investigation of Cas9 RGEN specificity in bacteria, the specificities of RGENs in human cells have not been extensively defined and, to our knowledge, bona fide off-target mutations induced by Cas9 have not been identified in any eukaryotic cell or organism. (cdc.gov)
Understanding the scope of RGEN-mediated off-target effects in human and other eukaryotic cells will be critically essential if these nucleases are to be used widely for research and therapeutic applications. (cdc.gov)

Subsequent in vitro and in vivo studies have demonstrated the initial steps of cancer cell invasion and dissemination by disrupting the integrity of the myoepithelial belt that encircles the LECs [ 7 , 8 , 9 , 10 , 11 ]. (biomedcentral.com)

11. Melchers, F.: Synthesis, transport and secretion of immunoglobulin in lymphoid cells. (uni-mainz.de)

Recognition of the effective involvement of the myoepithelial cells in mammary gland development and in hampering tumorigenesis has renewed the interest in investigating the biological roles of this second main mammary lineage. (biomedcentral.com)
The ductal system is coated by a chain of luminal epithelial cells (LECs) situated on a layer of myoepithelial cells (MECs) and encompassed by a distinguished basement membrane. (biomedcentral.com)
Two major cell types cooperate to build up the ductal units: inner polarized luminal epithelial cells (LECs) embraced by a layer of myoepithelial cells (MECs), both cell types are rimmed by a cohesive basement membrane (BM) [ 2 ]. (biomedcentral.com)
In this review, we provided an overview of the myoepithelial cells' histogenesis, molecular and biological markers, and physiological commitments. (biomedcentral.com)

In contrast, overexpression of USP19 increased cell invasiveness both in vitro and in vivo, further validating our findings. (nature.com)

From our screen, we identified the Ubiquitin-specific protease 19 (USP19) as a candidate gene associated with the regulation of cell migration. (nature.com)
In exocrine acinar cells regulation of intracellular calcium plays a pivotal role in controlling fluid and protein secretion. (rochester.edu)
A further project relates to the organization and regulation of calcium release sites in exocrine cells. (rochester.edu)
In: 'Regulation of the Immune System: Genes and the Cells in which They Function,' Vol. VI, eds. (uni-mainz.de)

The extracellular matrix (ECM) comprises a heterogeneous and versatile network of macromolecules synthesized and secreted by embryonic cells. (bvsalud.org)

RESULTS- Lcn2 is highly expressed by fat cells in vivo and in vitro. (diabetesjournals.org)
The major breakthrough is the identification of a biomarker of HIV persistent cells in vivo. (europa.eu)

GMS technique stains yeast cell walls brown-black as a result of deposition of reduced silver. (medscape.com)
In the serologic tests, four principal types of Candida antigens have been used, including (1) whole nonviable yeast cells, (2) candidal culture filtrates, (3) cell wall polysaccharides or glycoproteins, and (4) cytoplasmic antigens from mechanically disrupted yeast cells. (medscape.com)

Ras signaling is constitutively activated in a large fraction of human cancers [ 3 ]. (oncotarget.com)

2,284) were identified as harbor seals, and the remaining of decomposition were determined. (cdc.gov)
genic for harbor seals than for gray seals (9). (cdc.gov)

Nat Methods, 1 (3). (cshl.edu)
U2OS.EGFP cells caused by inactivating frameshift insertion/deletion (indel) mutations introduced by error prone non-homologous end-joining (NHEJ) repair of nuclease-induced double-stranded breaks (DSBs) (Fig. 1a and Methods ). (cdc.gov)

Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
[7] The complex cell signaling pathways of multicellular organisms such as plants and humans can make understanding the mechanisms of this inherited process very difficult. (cloudfront.net)

With up to a 91% sensitivity, PCR testing provides a much better chance at getting a positive result than culture does, if the patient harbors 1 of the 40, or fewer, organisms in that test panel. (urologytimes.com)
Carriers and patients with oral candidal infection can be distinguished reliably (95% confidence limits) on the basis of quantitative culture, since they harbor more than 400 colony-forming units of candidal organisms per mL of saliva. (medscape.com)

Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. (hindawi.com)
Dysfunctions of long non-coding RNA (lncRNAs) have been associated with the initiation and progression of hepatocellular carcinoma (HCC), but the clinicopathologic significance and potential role of lncRNA PTTG3P (pituitary tumor-transforming 3, pseudogene) in HCC remains largely unknown. (biomedcentral.com)
Mechanistically, over-expression of PTTG3P up-regulated PTTG1, activated PI3K/AKT signaling and its downstream signals including cell cycle progression, cell apoptosis and epithelial-mesenchymal transition (EMT)-associated genes. (biomedcentral.com)

At that time, biological rhythms were known for a variety of assemblages of cells but Sweeney noted that when such rhythms shifted, it was impossible to tell if all cells shifted or if the population became unsynchronized. (wikipedia.org)
Like other FGF family members, basic fibroblast growth factor possess broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development , cell growth , morphogenesis , tissue repair , tumor growth and invasion. (wikidoc.org)

Do features observed in specific cell-types or conditions tend to harbor trait-effecting variants, and can they inform our understanding of the trait etiology? (dana-farber.org)

In normal cells, glucose participates in cellular energy production through glycolysis as well as through its complete catabolism via the tricarboxylic acid (TCA) cycle and oxidative phosphorylation (OXPHOS). (hindawi.com)
HIV persistence may arise from ongoing residual virus replication and/or from latently-infected cells defined as the cellular reservoir in which long-lived resting memory CD4+ T cells harbouring an integrated but transcriptionally silent provirus represent the largest pool in the blood (Chomont et al. (europa.eu)
Our biochemical and cellular analysis thus demonstrates that the GID/CTLH complex prevents cell cycle exit in G1, at least in part by degrading Hbp1. (elifesciences.org)
FTS acts as a functional Ras antagonist in cells, affecting Ras-membrane interactions by dislodging the protein from its anchorage domains, facilitating its degradation and, thus, reducing cellular active Ras content [ 8 , 9 ]. (oncotarget.com)
Cell-mediated immunity to C albicans antigens can be demonstrated in most human subjects both by the appearance of delayed skin hypersensitivity to antigens and by in vitro tests of cellular immunity, such as inhibition of leukocyte migration or stimulation of lymphocyte transformation to candidal antigens. (medscape.com)

In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)

Available at: h ttps://pubmed.ncbi.nlm.nih.gov/30753992-exosomal-transmission-of-microrna-from-hcv-replicating-cells-stimulates-transdifferentiation-in-hepatic-stellate-cells/ [Accessed 25 Feb. 2020]. (bioscience.co.uk)

2002). At pH 2 to 3, at which the protein has a net charge low et al. (lu.se)

We used quantitative Western blotting to observe Lcn2 serum levels in lean and obese mouse models. (diabetesjournals.org)
[9] It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems. (wikidoc.org)

Cells from two severely affected children in family A harbor a homozygous point mutation in XPC intron 3 (-9 T to A), located within the downstream BPS. (yyu.edu.tr)

We compared the expression profiles of lncRNAs in 3 HCC tumor tissues and adjacent non-tumor tissues by microarrays. (biomedcentral.com)

For example, a major regulator of metabolism is phosphoinositol 3 kinase (PI3K). (hindawi.com)
In contrast to normal cells, most cancer cells predominantly produce energy by a high rate of glycolysis followed by lactate fermentation, even in the presence of oxygen, a less efficient metabolism compared to a low rate of glycolysis followed by mitochondrial oxidation of pyruvate [ 2 ]. (hindawi.com)
Salt accumulation by plant cells, with special reference to metabolism and experiments on barley roots. (jpmb-gabit.ir)

Interestingly, the combined treatment enhanced apoptotic cell death as indicated by increased sub-G1 cell population, increased Hoechst staining, activation of caspase 3, decrease in survivin expression and release of cytochrome c. (oncotarget.com)

8. Melchers, F.: Biosynthesis, transport and secretion of immunoglobulin in plasma cells. (uni-mainz.de)

Bioimpedance techniques have also been reported to detect of cell morphological changes in studies of ischemic rabbit liver tissue 7 and blood glucose monitoring 8 . (nature.com)
Typically, rapidly proliferating tumor cells have glycolytic rates up to 200 times higher than those of their normal tissue of origin, even in the presence of oxygen [ 3 ]. (hindawi.com)
Moreover, precise spatial (specific expression at the organ, tissue and cell levels) and temporal (developmental stage) expression of the transgene can minimize potential adverse effects or over-taxing the plant by producing unneeded products throughout the plant [ 17 ]. (biomedcentral.com)

Additionally, MECs contributed effectively to maintaining the right luminal cells' polarization and further separating them from the adjacent stroma by making an integrated fence. (biomedcentral.com)

Mol Cell Biol, 15 (11). (cshl.edu)
Semin Cell Dev Biol. (cornell.edu)

Activated Ras transmits signals through its interaction with multiple effectors, including mitogen activated kinase (MAPK), phosphoinositide-3-kinase (PI3K) and Ral-GEF [ 1 , 2 ]. (oncotarget.com)

Nucleic Acids Research, 26 (3). (cshl.edu)

Vaccination with designed neopeptides induces intratumoral, cross-reactive CD4+ T cell responses in glioblastoma. (uzh.ch)

Here, we use a human cell-based reporter assay to characterize off- target cleavage of Cas9-based RGENs. (cdc.gov)

Coordination of siderophore gene expression among clonal cells of the bacterium Pseudomonas aeruginosa. (uzh.ch)

Immunity in superficial candidosis and in oral candidosis is predominantly cell mediated. (medscape.com)

Anatomy22

Organisms40

Diseases9

Chemicals and Drugs39

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Psychiatry and Psychology2

Phenomena and Processes117

Disciplines and Occupations7

Anthropology, Education, Sociology and Social Phenomena3

Technology, Industry, Agriculture1

Information Science4

Health Care14

Geographicals4

Differentiation9

Biology4

Cold Spring9

Hybridization4

Tumors4

Epithelial cell3

Phoca3

Genetics2

Proliferation7

Genome3

Proteins7

Genetic4

Lymphocytes1

Mice3

Squamous5

Discovery1

Pancreatic1

Eukaryotic4

Invasion and dissemination1

Lymphoid cells1

Myoepithelial cells4

Vitro and in vivo1

Regulation4

Embryonic cells1

Vivo2

Yeast2

Cancers1

Seals2

METHODS2

Pathways3

Organisms2

Progression3

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Variants1

Cellular5

Stem1

20201

20021

Serum2

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Interestingly1

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Tissue3

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Predominantly1