Quantify protein detection. Medical search. Frequent questions

Itdetected PB1-F2 with the linear range extended from 10 to 500 nM, repeatability of 5% for 500 nM PB1-F2 and showed saturationof protein concentrations higher than 1 μM. (omicsonline.org)
There is greater imprecision and loss of accuracy at lower M-protein concentrations. (degruyter.com)
This study suggests that quantifying exceedingly low concentrations of M-proteins, although possible, may not yield adequate accuracy and precision between laboratories. (degruyter.com)
Allergen avoidance strategies including personal protective equipment, engineering controls, protein encapsulation, and reduction of airborne enzyme concentrations are required to mitigate occupational exposure to fungal enzymes. (cdc.gov)
our observations about the influence of polytherapies on lamotrigine pharmacokinetics confirm the relevance of quantifying this antiepileptic drug plasma concentrations in the process of treatment optimization. (bvs.br)

Here we describe how to detect nucleic acid-protein interactions in plant leaves by using a fluorescence resonance energy transfer (FRET) approach coupled to fluorescence lifetime imaging microscopy (FLIM). (nature.com)
To illustrate our approach, we provide experimental procedures to detect the interaction between plant DNA and two proteins (the AeCRN13 effector from the oomycete Aphanomyces euteiches and the AtWRKY22 defensive transcription factor from Arabidopsis ). (nature.com)
Enzyme-Linked Immunosorbent Assay (ELISA) uses antibodies and color change to detect and quantify proteins or other small molecules. (genscript.com)
GenScript offers a variety of ELISA kits to detect and quantify proteins and small molecule antigens in samples such as serum, plasma, saliva, cell culture supernatant, and urine. (genscript.com)
He developed the Eastern blotting technique to detect protein post-translational modifications. (mainlinehealth.org)
HPLC can detect and quantify amino acids in trace amounts. (pharmiweb.com)
Can detect specific amino acids based on unique absorbance spectra, thus providing valuable information about the concentration of amino acids in protein samples. (pharmiweb.com)
Further, T2* mapping, another parametric imaging approach, can detect and quantify myocardial iron deposition, primarily to assess iron overload cardiomyopathy. (medscape.com)

This analytical method quantifies trace levels of 12 aldehydes from protein adducts in human serum. (cdc.gov)
An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. (degruyter.com)
Serum protein electrophoresis (SPEP) is used to quantify the serum monoclonal component or M-protein, for diagnosis and monitoring of monoclonal gammopathies. (degruyter.com)
Due to the wide range of disease presentations, identification of the M-protein by serum protein electrophoresis (SPEP) may be the first clue to the diagnosis of a monoclonal gammopathy followed by subsequent isotyping via immunofixation (IFE) or immunosubtraction (ISUB). (degruyter.com)
GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunosorbent assay which can be used for quantitative detection of cAMP (Adenosine 3', 5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. (genscript.com)
GenScript Human IL-8 ELISA Detection Kit is a solid phase sandwich ELISA for quantitative measurement of human IL-8 in serum, plasma and cell culture supernatant. (genscript.com)
MATERIALS AND METHODS: We used a Concanavalin A-induced AIH mouse model and lipopolysaccharide-treated Raw264.7 cells to quantify serum biochemical indicators and confirm whether G13 exhibited protective effects in the AIH mice. (bvsalud.org)
Serum was separated for detection of the four parameters. (bvsalud.org)

Using therapeutic monoclonal antibody-spiked sera and a pooled beta-migrating M-protein, we aimed to assess SPEP limitations and variability across 16 laboratories in three continents. (degruyter.com)
Founded on the secretion of a monoclonal immunoglobulin (M-protein), plasma cell proliferative disorders are classified as monoclonal gammopathies and include multiple myeloma (MM), Waldenström's macroglobulinemia (WM), amyloidosis (AL), light chain deposition disease (LCDD), POEMS syndrome, and premalignant diseases such as monoclonal gammopathy of undetermined significance (MGUS) and smoldering multiple myeloma (SMM). (degruyter.com)
In monoclonal gammopathies, M-proteins are visualized as a restricted area of migration in the electrophoretic pattern which can be gated and quantified [ 4 ]. (degruyter.com)
To improve sensitivity, the researchers used monoclonal antibodies to purify each synaptic target protein from spinal fluid, then quantified each with mass spectrometry. (alzforum.org)

Because aldehydes have the tendency to react with biological molecules to form various products, including Schiff base protein adducts, free aldehydes released into the headspace of biological samples from the Schiff base protein adducts at low pH (~3) are analyzed. (cdc.gov)
3 , 4 Inflammatory molecules including C-reactive protein and inerleukin-6 are elevated in blood years before dementia. (neurology.org)
All the venomous species have a venom gland that synthesizes, stores and secretes a complex mixture of biological molecules including proteins. (nature.com)
Detection of target molecules is carried out with antibodies. (sysy.com)
a frequently employed method to quantify target-molecules in solution. (sysy.com)
The membranes feature very high retention of target proteins and molecules and a narrow molecular-weight cut-off variation. (dairyfoods.com)

The discipline of biophotonics deals with the interaction of light, or electromagnetic radiation, with living organisms and biologically active macromolecules: proteins (hemoglobin), nucleic acids (DNA and RNA), and metabolites (glucose and lactose). (nationalacademies.org)
In our present view, these areas are found for systems where several types of macromolecules (proteins, polysaccharides or polyelectrolytes) and/or amphiphiles interact to form a functional or destructive unit. (lu.se)

Analytes are then separated in a capillary column and quantified using multiple ion detection (MID) with mass spectrometry. (cdc.gov)
Reverse transcription polymerase chain reaction in conjunction with capillary electrophoresis and laser-induced fluorescence detection (RT-PCR/CE-LIF) was used to quantify hepatic expression of specific genes relative to that of b-actin in total RNA samples. (usda.gov)
CMR identifies areas of increased interstitial space where gadolinium is retained (eg, areas of myocardial infarction, acute edema/inflammation due to capillary leak, myocardial fibrosis/scar, or amyloid protein infiltration). (medscape.com)

Scientists at the UK's National Physical Laboratory have developed a new strategy for quicker and more precise detection of biomarkers - proteins which indicate disease. (medicaldaily.com)

Produced by a variety of cell types, extracellular vesicles (EVs) are able to transfer lipids, nucleic acids and proteins to recipient cells. (nature.com)

For Lexington, Massachusetts-based Quanterix, which to date has deployed assays for protein analysis on its Single Molecule Array, or Simoa, platform, the approach demonstrates the applicability of its technology in nucleic acid detection, and miRNA detection in particular, as it prepares to launch a new system. (genomeweb.com)
Pull-down assays between PRPF31 and PRPF6 proteins showed a stronger interaction for the mutant than wild type, suggesting a mechanism for the negative effect. (molvis.org)
These assays are commercially available for detection of bacterial and viral pathogens, including HIV-1, cytomegalovirus, enteric viruses, Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycobacterium tuberculosis . (medscape.com)

The released phenolic acids and lipids were identified and quantified using HPLC with detection by both UV and evaporative light-scattering detection (ELSD). (usda.gov)
1985) and in rat urine by high-performance liquid chromatography (HPLC) with ultraviolet (UV) absorbance detection following formation of the 2,4-dinitrophenylhydrazone derivative (Shara et al. (cdc.gov)
HPLC can be combined with various detection methods, such as UV absorption or fluorescence, to improve the accuracy of the analysis. (pharmiweb.com)
IEC, HPLC or CE) and detection methods described above to identify and quantify amino acids in protein samples. (pharmiweb.com)

The expressed protein in Escherichia coli was used to generate polyclonal antibodies. (hindawi.com)
Immunoassays rely on specific antibodies to bind a target biomarker, and on a detection system (usually optics) to quantify it. (bosch.com)
If you want good linearity in quantitation, use a 96-well chemoluminescence counter and a chemoluminescent detection kit for HRP or AP-labeled secondary antibodies. (bio.net)

Our results have also shown that "pure" CFG fractions often contain considerable amounts of associated lipids, phenolic acids and proteins which contribute to its emulsifying properties. (usda.gov)
The extraction of CFG with alkaline hydrogen peroxide was investigated using different combinations of alkali concentration and time to identify the optimum extraction condition to retain its functional groups (protein, lipids and phenolic acids). (usda.gov)
The total polar lipids, phenolic acids and protein content in CFGs isolated with lower alkali concentration for a shorter time was comparatively higher than CFGs isolated with higher alkali concentration for a longer time. (usda.gov)
The presence of these phenolic acids, lipids and protein in CFG may contribute to its excellent emulsifying properties and may combine to give other chemical, physical, and even nutritional properties. (usda.gov)
Once produced within a cell, free radicals can damage a wide variety of cellular constituents, including proteins, lipids and DNA. (enzolifesciences.com)

The method reproducibly hydrolyzes aldehyde adducts covalently bound to proteins, using automation to individually incubate samples prior to analysis with hydrochloric acid (Yeo 2004). (cdc.gov)

Objective: The aim of the study was to characterize the type of response to different HCV antigens, quantify HCV viral load, transforming growth factor- beta and miRNA 122 in patients with newly diagnosed Hepatocellular Carcinoma. (bvsalud.org)

After analysis of deduced amino acid sequences from cloned cDNAs of dipeptidyl peptidase IV (DPP IV) and aminopeptidase A (APA), it was noticed that DPP IV and APA are type II membrane proteins that have one transmembrane domain near the N-terminus. (nature.com)
Dr. Thomas modeled the membrane protein of SARS-CoV-2 and showed that it functions as a sugar transporter. (mainlinehealth.org)
separation of proteins by PAGE and subsequent transfer to a membrane. (sysy.com)
The sample is passed over a protein-binding membrane in a 96-well vacuum manifold, that way it is distributed over a defined area. (bio.net)
in poliovirus (PV), the interaction between the RNA replication complex and intracellular membranes appears to be accomplished by proteins 3A and 2C, which have membrane-binding properties ( 11 , 60 ). (asm.org)

Foot-and-mouth disease virus (FMDV) nonstructural protein 3A plays important roles in virus replication, virulence, and host range. (asm.org)
The viral genome encodes four structural capsid proteins (VP1 to VP4) and seven nonstructural (NS) proteins, the leader Lb/ab protease, and proteins encoded in the P2 (2B and 2C) and P3 (3A, 3B, 3C, and 3D) regions ( 9 ). (asm.org)

Binding is quantified using fluorescence-based detection methods. (wikipedia.org)
Information-rich methods are required to quantify the relative levels of various reactive species. (enzolifesciences.com)
Additionally, analytical methods are included that modify previously used methods to obtain lower detection limits and/or to improve accuracy and precision. (cdc.gov)
The amino acids are eluted in a specific order and their concentration can be determined by various detection methods. (pharmiweb.com)

Protein collected from cells infected or uninfected using RIPA lysis buffer. (cdc.gov)
NS proteins are involved in crucial aspects of the viral cycle and pathogenesis, such as rearrangements of intracellular membranes required for endomembrane recruitment and the lysis of host cells ( 1 , 12 , 14 , 18 , 73 ). (asm.org)

Protein amino acid analysis is a technique for determining the type and amounts of amino acids in a protein sample, providing information on protein composition, post-translational modifications and overall protein quality. (pharmiweb.com)
By gaining insight into amino acid content, researchers can assess protein purity, determine changes in amino acid composition, and evaluate protein stability. (pharmiweb.com)
What is Protein Amino Acid Analysis? (pharmiweb.com)
Automated instruments specifically designed for protein amino acid analysis. (pharmiweb.com)
The ionizable amino acid side chains of proteins are usually located at the surface. (lu.se)

However, these proteins occur in very low levels in cerebrospinal fluid (CSF), making them difficult to measure. (alzforum.org)
Other "in-house" PCRs have been developed at individual laboratories to diagnose infections (eg, testing of cerebrospinal fluid [CSF] for herpes simplex virus to diagnose herpes encephalitis and testing of nasopharyngeal wash fluid to diagnose Bordetella pertussis infection ), particularly if traditional culture and antigen detection techniques have failed. (medscape.com)

This parallel detection of PB1-F2 and NP suggests that applied sensor chip technology may be amenable to an arrow immunosensor for simultaneous detection of all known influenza virus proteins in infected tissues and cells. (omicsonline.org)
At last, the mRNA and protein expression of NFAT2, Egr2, FasL, COX-2 and c-myc in carcinoma and adjacent tissues was investigated. (researchsquare.com)
Immunofluorescent staining can localize and quantify protein expression in cells or tissues. (cdc.gov)

The immunosensor was successfully applied in the detection and quantification of PB1-F2 in infected mouse lungs and cell lines, providing temporal expression profiles of PB1-F2 during viral infection. (omicsonline.org)
It provides a great solution for routine BSA impurity detection, quality control and lot release, as well as purification process optimization during viral vector and vaccine production. (genscript.com)
The viral particle is composed of a protein capsid that contains a positive-sense RNA molecule of about 8,500 nucleotides that is infectious and encodes a single polyprotein, which is processed in infected cells by cis - and trans -acting viral proteases ( 55 ) to yield different polypeptide precursors and the mature viral proteins ( 9 , 62 ). (asm.org)
HCV is thought to induce HCC either indirectly or directly by the effect of its viral proteins on different host cell proteins and signaling pathways. (bvsalud.org)

An automated analytical method was developed using solid phase microextraction (SPME) gas chromatography (GC) and high-resolution mass spectrometry (HRMS) with selected ion mass detection and isotope-dilution techniques. (cdc.gov)
The method was validated in terms of retention time stabilities, limits of detection, linearity, recovery, and analytical accuracy. (cas.cz)

The genetic study showed promising results in "silencing" the genes that translate into tau protein, the primary component behind dementia. (medicaldaily.com)
Expression of sterol regulatory element binding protein 1 (SREBP-1), ATP-citrate lyase (ACL), fatty acid synthase (FAS), malic enzyme (ME), acetyl-CoA carboxylase (ACC), and stearoyl-CoA (?9) desaturase 1 (SCD1) genes in ad lib birds declined from their highest levels just prior to photo-stimulation to reduced levels as the birds came into and maintained egg production. (usda.gov)
Hepatic expression of fatty acid binding protein (FABP), very low density apolipoprotein II (ApoVLDL-II) and apolipoprotein B (ApoB) genes increased in both ad lib and restricted breeders following photo-stimulation, whereas apolipoprotein A1 (ApoA1) gene expression declined in both groups. (usda.gov)

Replacements L38E and L41E, involving charge acquisition at residues predicted to contribute to the hydrophobic interface, reduced the dimerization signal in the protein ligation assay and prevented the detection of dimer/multimer species in both transiently expressed 3A proteins and in synthetic peptides reproducing the N terminus of 3A. (asm.org)
Desthiobiotin binding to streptavidin is easily reversible under acidic elution conditions, allowing high recovery of labeled proteins and peptides. (thermofisher.com)

According to the researchers, the assay had a limit of detection of 500 femtometers, leading the authors to claim it was about "500 times more sensitive" than an unnamed analog, bead-based assay. (genomeweb.com)
The ROS-ID ® ROS/RNS detection kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. (enzolifesciences.com)
This kit also offers a His-tagged protein stock for customer recovery verification assay. (genscript.com)
This type of assay is linear over 5-6 orders of magnitude, the common colorimetric or photographic detection not even for one order of magnitude. (bio.net)
In this work, FMDV 3A homodimerization was evidenced by an in situ protein fluorescent ligation assay. (asm.org)
Then, the chemical count kit-8 cell viability assay, Annexin V-FITC apoptosis detection, EdU labeling proliferation detection, transwell and wound healing experiments were performed. (researchsquare.com)
We used enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction to quantify the inflammatory factors. (bvsalud.org)

In this study, the sp2 gene was cloned and expressed in Escherichia coli to produce a 6xHis-tagged fusion protein, which was then used to generate a polyclonal antibody. (hindawi.com)
So I'd confirm by Western that there is only one protein reacting with the antibody, but for quantitation I'd use dot blots. (bio.net)

The investigators used liquid chromatography-mass spectrometry, a sensitive detection technology, to quantify gluten content. (eurekalert.org)
We describe here a new reversed-phase high-performance liquid chromatography with mass spectrometry detection method for quantifying intact cytokinin nucleotides in human K-562 leukemia cells. (cas.cz)
Gas chromatography is suitable for the analysis of complex protein samples. (pharmiweb.com)
Dr. Allen's group uses GC/MS (Gas Chromatography coupled with Mass Spectrometric Detection) for quantifying the total lipid content in samples obtained from algae biofuels companies who are developing cost-effective ways to optimize growth and harvesting techniques to convert the lipid fraction of the algae biomass into various types of biofuels. (liberty.edu)

Figure 3: GFP fluorescence observed in Nicotiana leaves that express GFP-tagged proteins. (nature.com)
Enzo Life Sciences ROS-ID® ROS/RNS detection kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy. (enzolifesciences.com)
Some of the modern detection systems for fluorescence may offer similar linearity, but I haven't layed my hands on one of those yet. (bio.net)

Through the combination of the three detection probes with a set of specific inhibitors and activators, this kit enables discrimination among superoxide, nitric oxide and peroxynitrite. (enzolifesciences.com)
As many kinases, GTPases, and other nucleotide binding proteins bind nucleotides or inhibitors even when they are enzymatically inactive, these reagents allow both inactive and active enzymes to be profiled in a complex sample. (thermofisher.com)

DNA-binding proteins (DNA-BPs) and RNA-binding proteins (RNA-BPs) have critical roles in living cells in all kingdoms of life. (nature.com)
Proteins of interest (POI) are tagged with a GFP and transiently expressed in plant cells to serve as donor fluorophore. (nature.com)
Here, we report a surface enhanced Raman scattering technology for rapid and label-free exosomal detection (Exo-SERS) to aid in the discrimination of different cancer cells based on specific Raman phenotypes and multivariate statistical analysis. (mdpi.com)
Representative experiment demonstrating peroxynitrite and NO generation in HeLa cells using the ROS/RNS Detection Kit. (enzolifesciences.com)
ROS-ID ® ROS/RNS detection kit is used to stain HeLa cells using the procedures described in the user manual. (enzolifesciences.com)
He has also contributed to cell biology: described fougaro system in cells, anigma in the muscularis of the colon and peinosis of the colon when on a high fat high protein diet. (mainlinehealth.org)
It was noted by the authors that this method was free from interferences from proteins and bacterial cells so it might have applicability to biological fluids such as blood or urine. (cdc.gov)
In close agreement, induction of GCC signaling in mucosal sheets ex vivo and intestinal cells in vitro inhibited proliferation by activating cGMP-dependent protein kinase and delaying the cell cycle at the G1/S transition. (jefferson.edu)
These cells do not express the ASC protein and display a complete abrogation of mature IL-1β secretion upon activation of the canonical and non-canonical inflammasomes. (invivogen.com)
For detecting and quantifying the release of mature human (h)IL-1β, InvivoGen provides HEK-Blue™ IL-1β sensor cells , which express an NF-κB-inducible SEAP reporter gene. (invivogen.com)
When expressed as a recombinant protein in transfected cells, PV 3A cofractionates with endoplasmic reticulum markers ( 66 ), and its single transient expression can disrupt the secretory apparatus ( 23 ) and decrease major histocompatibility complex (MHC) class I expression ( 22 ). (asm.org)
Moreover, we recently reported that lycopene inhibits the metastasis of human liver adenocarcinoma SK-Hep-1 cells by downregulation of the NADPH oxidase 4 (NOX4) proteins ( 18 ). (frontiersin.org)
Immunofluorescent double-labeling with e-cadherin can be used with immunofluorescent detection of podoplanin, a lymphatic endothelial cell marker that is also expressed by alveolar type I cells in the lung. (cdc.gov)

Subsequent elution at basic pH enabled detection of low picomolar p217+tau levels in human CSF (lower limit of quantification: 2 pM), resulting in an approximate 5-fold increase in sensitivity. (quanterix.com)
GenScript His Tag Protein Detection Kit is developed for the detection of His-tagged protein expression and its relative quantification using His-tagged protein standards of known molecular weight (M.W.). This kit is based on competitive ELISA, suitable for most systems, such as bacterial, yeast, mammalian cell lysates and cell culture supernatant. (genscript.com)
The column separates the amino acids based on their hydrophobicity, allowing for their individual detection and quantification. (pharmiweb.com)
Once separated by LC, the amino acids enter the mass spectrometer for detection and quantification. (pharmiweb.com)

In addition, the molecular function of some of the described proteins suggests a central role for SVEVs in the cytotoxicity of the snake venom and sheds new light in the envenomation process. (nature.com)

This method employs isotope dilution to quantify precisely trace amounts of aldehydes with limits of detection in the low parts per trillion range. (cdc.gov)

This technology provides the means of studying glycan-protein interactions in a high-throughput environment. (wikipedia.org)

ab134002 is for simultaneous detection of 41 Human Growth Factors. (abcam.com)

Proteins were quantified by BCA method. (cdc.gov)
The protein content was determined by AACC approved combustion method. (usda.gov)
Sixteen laboratories reported the perpendicular drop (PD) method of gating the M-protein, while 10 used tangent skimming (TS). (degruyter.com)
For the protein precipitation and sample preparation, a trichloroacetic acid extraction method is used. (cas.cz)

Glycan array screening works in much the same way as other microarray that is used for instance to study gene expression DNA microarrays or protein interaction Protein microarrays. (wikipedia.org)
GFP fusion protein for which expression is driven by an NF-κB-inducible promoter. (invivogen.com)
Since many of these enzymes are expressed as inactive pro-proteins, the ability of these probes to assess activity is especially advantageous over traditional protein or RNA expression profiling techniques which only measure abundance. (thermofisher.com)
NFAT2, as an important transcriptional promoter, regulates expression of TNF-α, myc proto-oncogene protein (c-myc), cyclooxygenase-2 (COX-2), Fas ligand (FasL) and also generates crosstalks with ERK/MAPK pathway and AKT/GSK3b signaling, which achieves its control of the cell fate [7-12]. (researchsquare.com)
E-cadherin immunofluorescent double labeling can also be used with activated caspase 3 or â-catenin immunofluorescence to localize the expression of these proteins in damaged airways. (cdc.gov)

Mechanistically, we found that lycopene and sorafenib could additively inhibit the mitogen-activated protein kinase (MAPK) pathways, as shown by the protein phosphorylation of ERK1/2, JNK1/2 and p38 were reduced additively. (frontiersin.org)

Molecular docking, immunofluorescence, and western blotting experiments of G13 and key proteins of the NF-κB/MAPK pathway revealed that G13 alleviated inflammation. (bvsalud.org)

Hudson, W.H. & Ortlund, E.A. The structure, function and evolution of proteins that bind DNA and RNA. (nature.com)

ELISA Detection Kits contain all necessary reagents and buffers for performing 96-well plate ELISA. (genscript.com)
GenScript GST tag ELISA Detection Kit is designed to quantitate glutathione-S-transferase (GST) or GST-fusion protein in samples such as cell lysate using a proprietary sandwich ELISA procedure in just 60 minutes. (genscript.com)

Glutamate excitation induced a rapid alteration in the AMP:ATP ratio that was associated with the activation of the AMP-activated protein kinase (AMPK). (jneurosci.org)
Notably, inhibition of the CaMKK (calmodulin-dependent protein kinase kinase) had little affect on GLUT translocation, whereas the inhibition or knockdown of AMPK (compound C, siRNA) activity prevented GLUT3 translocation to the cell surface after glutamate excitation. (jneurosci.org)

The kit includes three detection probes: NO Detection Reagent (Red, Ex/Em 650/670 nm), Oxidative Stress Detection Reagent (Green, Ex/Em 490/525 nm) for total ROS detection, and Superoxide Detection Reagent (Orange, Ex/Em 550/620 nm). (enzolifesciences.com)
Excitation and emission spectra of Oxidative Stress Detection Reagent (green), Superoxide Detection Reagent (blue), and NO Detection Reagent (red). (enzolifesciences.com)
The activity of the Lucia luciferase reporter protein can be readily assessed using the QUANTI-Luc™ detection reagent. (invivogen.com)

Owing to its high-spatial resolution and 3D tomography, CMR provides superior myocardial border definition and volumetric analysis, accurate determination of regional wall thickness and myocardial mass, and sensitive detection of WMA. (medscape.com)

Nod-like receptor protein 3 (NLRP3) is a protein complex composed of multiple proteins whose formation activates cysteine aspartate protease-1 (caspase-1) to induce the maturation and secretion of inflammatory mediators such as interleukin (IL)-1ß and IL-18, promoting the development of inflammatory responses. (bvsalud.org)

Because a uniform dielectric ionizable group in a protein relative to the pKa value of the continuum model is a very crude description of the highly same group in a small reference molecule in dilute aqueous heterogeneous protein interior, the dielectric coefficient, eP, solution. (lu.se)

The detection and evaluation of concentration of influenza virus proteins in biological samples is critical in a broad range of medical and biological investigations regarding the concern over potential outbreaks of virulent influenza strains in animals and humans. (omicsonline.org)
Gamma globulin background, migration pattern and concentration all affect the precision and accuracy of quantifying M-proteins by SPEP. (degruyter.com)

A family of protein-deglutamylating enzymes associated with neurodegeneration. (sysy.com)
B. Structures of azido, desthiobiotin and fluorescent fluorophosphonate probes for labeling, affinity enrichment or fluorescent detection of labeled enzymes. (thermofisher.com)

The use of real-time quantitative RT-PCR to quantify specific mRNAs allows for more rapid testing, higher sensitivity, increased simplicity, and more accuracy. (medscape.com)

Analytes were quantified in negative selected ion monitoring mode using a single quadrupole mass spectrometer. (cas.cz)
The limits of detection for the analytes vary from 0.2 to 0.6 pmol. (cas.cz)

QUANTI-Blue™ Solution allows rapid colorimetric detection and measure of SEAP activity by reading the optical density at 630-650 nm. (invivogen.com)

SVEVs isolated from lyophilized venoms collected from four different species of snakes ( Agkistrodon contortrix contortrix , Crotalus atrox , Crotalus viridis and Crotalus cerberus oreganus ) were analyzed by mass spectrometry-based proteomic, which allowed the identification of proteins belonging to eight main functional protein classes such as SVMPs, serine proteinases, PLA 2 , LAAO, 5′nucleotidase, C-type lectin, CRISP and Disintegrin. (nature.com)
Missense mutations in PRPF31 have previously been shown to cause reduced protein solubility, suggesting insufficiency of functional protein as the disease mechanism. (molvis.org)

Various experimental approaches exist for the study of nucleic acid-protein interactions in vitro and in vivo , but the detection of such interactions at the subcellular level remains challenging. (nature.com)

For Edinburgh, UK-based Destina Genomics, meantime, the study showcases the use of its chemistry for the PCR-free detection of miRNA on a commercial system. (genomeweb.com)

Storage protein 2 (SP2) not only is an important source of energy for the growth and development of silkworm but also has inhibitory effects on cell apoptosis. (hindawi.com)
A recent study showed that another protein in silkworm hemolymph, storage protein 2 (SP2), can also inhibit staurosporine-induced HeLa cell apoptosis and ROS generation [ 8 ]. (hindawi.com)
ASC ( apoptosis-associated speck-like protein containing a CARD domai n, also known as PYCARD) is a protein adaptor important in canonical inflammasome responses [1,2]. (invivogen.com)

2 The GTP probe specifically labels small GTPases and G-protein coupled receptor GTPase subunits. (thermofisher.com)

The current MCAT regularly has questions which require previous knowledge of the structures, features (including changes in charge with pH), 3- and 1-letter abbreviations of the 20 common protein-generating amino acids, etc. (mcat-prep.com)
This involves protein extraction from the biological sample of interest, followed by protein denaturation to break down the tertiary structure and expose the amino acids. (pharmiweb.com)
Once the protein is denatured, it needs to be hydrolyzed to release individual amino acids. (pharmiweb.com)
The hydrolysis process breaks the peptide bonds, converting proteins into their constituent amino acids. (pharmiweb.com)
Amino acids are generally not very amenable to direct detection by LC-MS due to their low ionization efficiency. (pharmiweb.com)

In three small, independent cohorts, levels of these synapse proteins were higher in people with Alzheimer's dementia than in controls, the scientists reported. (alzforum.org)

Provided with total protein (reverse biuret, Siemens), laboratories blindly analyzed samples according to their SPEP and immunofixation (IFE) or immunosubtraction (ISUB) standard operating procedures. (degruyter.com)
As the background increases, imprecision increases and accuracy decreases leading to overestimation of M-protein quantitation especially evident in hypergamma samples, and more prominent with PD. (degruyter.com)
This refinement allowed the detection of the proteins in CSF samples from individual patients, according to the new papers. (alzforum.org)

We investigated the use of e-cadherin immunofluorescence in different species (rat and mouse), with different fixatives (10% neutral buffered formalin, 4% paraformaldehyde, and 2.5% glutaraldehyde ), with and without antigen retrieval, and as a dual label with immunofluorescence for other proteins. (cdc.gov)

This study shows that proteins can be secreted and confined in snake venom extracellular vesicles (SVEVs) presenting a size distribution between 50 nm and 500 nm. (nature.com)

These proteins are responsible for inducing several pharmacological effects aiming to immobilize and initiate the pre-digestion of the prey. (nature.com)

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