Residual biofilms were analyzed by quantifying the viable cells (CFU/mL), and biofilm architecture was evaluated by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). (fapesp.br)
The study enabled us to visualize the active bacteria like never before using Fluorescence-in-situ-Hybridization (FISH) of 16S rRNA and Confocal Laser Scanning Microscopy (CLSM). (thesecretlifeofskin.com)
Scanning electron microscopy (SEM) has been successfully used to image biofilms because of its high resolution and magnification. (springeropen.com)
Many early biofilm studies used scanning electron microscopy (SEM), which showed in detail the surface morphologies of microbial biofilms and their structure. (springeropen.com)
The results of objects present in the closed system cannot be obtained using conventional techniques such as the Raman microscopy, confocal laser scanning microscopy (CLSM) or other microscopy methods. (uwm.edu)
The unique geochemical coupling of organic molecules and mineral CaCO3provides a fluorescence signature detectable using conventional confocal scanning laser microscopy (CSLM). (ad-astra.ro)
Using confocal laser scanning microscopy (CLSM) to visualize both living and dead bacterial cells, our researchers can study how the CNT concentration in the nanocomposite affects the viability of microbes that are exposed to the surface of the plastic for short periods of time. (jhu.edu)
SEM and Confocal laser scanning electron microscopy (CLSM) are used to analyze changes in the biofilm integrity as a result of treatment [ 20 ] . (encyclopedia.pub)
Then, we use confocal microscopy experiments and Monte Carlo simulations spanning a broad range of volume fractions and attraction strengths to show that disordered clusters form near coexistence boundaries, whereas oriented strands persist with strong attractions. (nature.com)
Morphological changes of the bacterial cells were visualized by transmission electron microscopy (TEM) and scanning transmission electron microscopy with energy-dispersive X-ray spectroscopy (SEM EDS) analysis. (bvsalud.org)
Further verifications were carried out using proteomic tools, immunoblotting, and confocal laser scanning microscopy. (biomedcentral.com)
For the first time, people could directly see the bacteria sitting inside a kitchen sponge, forming biofilm-like structures. (thesecretlifeofskin.com)
Our second objective was to determine whether Nocardia bacteria could form a biofilm on CVCs in a laboratory model and whether biofilm growth could be prevented with the use of antimicrobial lock solutions. (cdc.gov)
Biofilm bacteria attach to surfaces and are surrounded by an extracellular matrix called the extracellular polymeric substance (EPS) (Costerton et al. (springeropen.com)
In environmental contexts, bacteria often colonize surfaces as biofilms, so Bouwer group researchers are also studying the longer-term effects of exposure to CNT-PNC surfaces on biofilm formation in a drip-flow reactor. (jhu.edu)
Aeruginosa biofilms1
0.01) reduction was demonstrated on micro-patterned surfaces for P. aeruginosa biofilms under clinically-simulated conditions when compared to un-patterned controls. (springeropen.com)
Microbial2
The EPS has many functions, such as promoting the microbial adhesion and structural stability of the biofilm (Flemming et al. (springeropen.com)
To evaluate the feasibility of this micro-pattern for this application, the microbial range of performance was investigated in addition to biofilm studies with and without a mucin-rich medium to simulate the tracheal environment in vitro . (springeropen.com)
Vitro4
This in vitro study evaluated the efficacy of citric acid denture cleanser treatment on C. albicans biofilm recolonization on poly(methyl methacrylate) (PMMA) surface. (fapesp.br)
Nocardia promoted heavy biofilm formation on the surface of central venous catheter segments tested in an in vitro biofilm model. (cdc.gov)
Trimethoprim- and minocycline-based lock solutions had potent in vitro activity against biofilm growth. (cdc.gov)
This engineered micro-pattern reduces the colonization and biofilm formation of key VAP-associated pathogens in vitro . (springeropen.com)
Pathogens2
Two key pathogens, MRSA and Pseudomonas aeruginosa, were evaluated for biofilm formation in a nutrient rich broth for four days and minimal media for 24 hours, respectively, on each surface type. (springeropen.com)
Frequent recurrent lung infections result in irreversible lung damage in children with cystic fibrosis (CF). This study aimed to determine if toothbrushes contain biofilms of pathogens, and act as potential reservoirs for lung re-infection following antibiotic treatment of acute exacerbations. (researchgate.net)
Antimicrobial1
Staphylococcus aureus and Pseudomonas aeruginosa were used for antimicrobial efficacy evaluation (based on DIN EN 13727). (ewco.com)
Thickness1
In this study, voltage and phase shifts were detected in materials with thickness greater than 40 µm, indicating that the sensor arrangement can be used to detect biofilm of thickness greater than 40 µm in a closed piping system. (uwm.edu)
Bacterial cells1
Direct SEM observation clearly revealed that bacterial cells in a biofilm were extensively surrounded by fibrous or amorphous matrices, which represented the EPS (Marrie and Costerton 1984 ). (springeropen.com)
Electron microscope1
However, when using a conventional scanning electron microscope, the sample chamber must be kept dry and under high vacuum during use, so the specimens are limited to nonvolatile, conductive materials (Tsuda et al. (springeropen.com)
Conventional4
However, conventional SEM requires dehydration and metal coating of biological samples before observation, and because biofilms consist mainly of water, sample dehydration may influence the biofilm structure. (springeropen.com)
Compared with samples prepared by the conventional method, the ionic-liquid-treated samples do not exhibit a fibrous extracellular matrix structure and cracking on the biofilm surface. (springeropen.com)
To avoid the draconian process of total sample dehydration required in conventional SEM, environmental SEM (ESEM), which can be used to visualize specimens in a wet or partially hydrated state, has been used to observe biofilm samples (Darkin et al. (springeropen.com)
Analyze1
Introduction Flow cytometry is a powerful laboratory technique used in the fields of biology, medicine, and immunology to analyze and quantify various physical and chemical characteristics of cells and particles. (medicallabnotes.com)
Microorganisms2
The billions of microorganisms form hard-to-crack biofilm-like structures inside a kitchen sponge and probably all domestic sanitization methods eventually fail in sterilizing a sponge. (thesecretlifeofskin.com)
Biofilms are formed when a group of cells of microorganisms stick to each other and often on a surface. (uwm.edu)
Efficacy3
In addition, the long-term efficacy of this denture cleanser is not well established, and their effect on residual biofilms is unknown. (fapesp.br)
The product that lost the most efficacy due to biofilm was 1 chlorhexidine, while 1 PVP-I and ClHO at either 300 or 500 mg/L were moderately affected by biofilm. (ewco.com)
Formation6
Biofilm formation causes prolonged wound infections due to the dense biofilm structure, differential gene regulation to combat stress, and production of extracellular polymeric substances. (ewco.com)
There is an immediate need for new technologies, that are both real-time and non-invasive that can be used to quantify biofilm formation in closed systems, which can reduce the loss incurred by the healthcare, food, chemical and water industry. (uwm.edu)
along with their ability to promote biofilm formation capability of the host. (wikipedia.org)
The ETT provides a conduit for bacterial access to the lower respiratory tract and a substratum for biofilm formation, both of which lead to VAP. (springeropen.com)
P. aeruginosa was further evaluated for biofilm formation on each surface type in a mucin-modified medium mimicking tracheal mucosal secretions. (springeropen.com)
Biofilm formation was also reduced, with 67% ( p = 0.12) and 52% ( p = 0.05) reductions in MRSA and P. aeruginosa biofilm formation, respectively. (springeropen.com)
Infections2
In the medical field alone, biofilm infections have reportedly cost over five billion USD in additional healthcare expenses. (uwm.edu)
As biofilms are known to play a role in mesh-related infections, we investigated the presence of biofilms on hernia meshes, which had to be explanted due to mesh failure without showing signs of bacterial infectio. (researchgate.net)
Methods2
Methods: C. albicans biofilms were developed for 72 h on PMMA resin specimens (n = 168), which were randomly assigned to 1 of 3 cleansing treatments (CTs) overnight (8 h). (fapesp.br)
PurposeTo assess 7-year outcomes after complex ventral hernia (CVH) repair using pre-operative Botulinum toxin A (BTA) injection and the Macquarie System of management.Methods Clinical examination and functional non-contrast abdominal CT scans were used to assess complications and recurrences encountered in a prospective series of 88 consecutive CV. (researchgate.net)
Surface1
For example, early reports of the imaging of DNA in scanning tunneling microscopes (STMs) used highly ordered pyrolitic graphite (HOPG) substrate, and subsequent investigations showed that at least some of the features reported were not those of DNA at all but of artifacts on the HOPG surface. (nationalacademies.org)
Concentrations3
The most effective in the presence of biofilm was ClHO at 1500 mg/L. ClHO at mediumlow concentrations (300 or 500 mg/L) is a good antiseptic that can be used on wounds and mucous membranes for 510 min. (ewco.com)
Lower concentrations of ClHO, as well as of the other antiseptics studied, were less effective or more altered by the biofilm. (ewco.com)
Starting with an easily biodegradable polymer known as PCL (poly-ε-caprolactone), we add CNTs to form a nanocomposite with a range of nanotube concentrations so that we can quantify how the CNT loading affects the biodegradability of the PCL under various conditions. (jhu.edu)
Presence2
ClHO at a concentration of 1500 mg/L is very effective in the presence or absence of biofilm that can be used on healthy skin for 5 min. (ewco.com)
The food industry usually halts the operation of its plant eight hours, every day to ensure that their equipment and transportation channels are clean and free from any biofilm presence. (uwm.edu)
Biological1
In the present study, diverse cell biological and analytical tools such as laser ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS), isotope labeling, and nano-scale secondary ion mass spectrometry (NanoSIMS) were employed to better understand the distribution and activation process of KP2156 (in comparison to free oxaliplatin and a non-albumin-binding succinimide analogue). (microbial-ecology.de)
Time1
Residual biofilms adhering to the specimens were collected and quantified at two time points: immediately after CTs (ICT) and after cleaning and residual biofilm recolonization (RT). (fapesp.br)
Images1
The hydrophilic ionic liquids give clearer images of the biofilm structure than those of the hydrophobic ionic liquids. (springeropen.com)
Mature1
and particularly those conditions associated with a mature anaerobic biofilm. (wikipedia.org)
Clinical1
I am currently leading a UK multi-centre clinical trial evaluating photodynamic laser treatment for central serous chorioretinopathy funded by the NIHR and an international research project funded by the Wellcome Trust evaluating machine learning as a tool to better understand age related macular degeneration (AMD). (southampton.ac.uk)
Study2
This study finds that ionic liquids are useful for allowing the observation of biofilms by SEM without preparation by dehydration and metal coating. (springeropen.com)
Purpose The purpose of this study was to investigate the link between bacterial biofilms and negative outcomes of hernia repair surgery. (researchgate.net)
Treatment1
Biofilm on Toothbrushes of Children with Cystic Fibrosis: A Potential Source of Lung Re-Infection after Antibiotic Treatment? (researchgate.net)
Major2
1999 ). Ninety percent of the dry weight mass of biofilms is composed of EPS, and the other major component of the biofilm matrix (up to 97%) is water (Sutherland 2001 ). (springeropen.com)
The development of biofilm has been a major issue in many fields (medical field, food, chemical, and water industry are a few such fields). (uwm.edu)
Skin1
For cytotoxicity evaluation (based on DIN EN 10993-5), human keratinocytes (HaCaT) and human skin fibroblasts (BJ) were used. (ewco.com)
Host1
Cytotoxicity and histopathological assessment showed no discernible harm to host tissues when e-scaffolds were applied to explant biofilms. (ewco.com)
Water1
Similarly, the water and chemical industry need to ensure that their transportation channels are free from biofilm build-up to ensure that the flow rate of liquid flowing through the channels is neither affected nor contain bacterial traces. (uwm.edu)