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  • isozyme
  • The enzyme activities and isozyme distribution of the three glycolytic regulator enzymes hexokinase, phosphofructokinase and pyruvate kinase were studied in lymphocytes of patients with chronic lymphocytic leukemia. (uu.nl)
  • isoform
  • Sequences that would target the M2 isoform of pyruvate kinase specifically were identified ( Fig. 1 A ). A custom library of siRNAs that tiled exon 10, which is included exclusively in the M2 isoform, was screened ( Table S1 ). (rupress.org)
  • The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. (nih.gov)
  • Tumour cells have been shown to express exclusively the embryonic M2 isoform of pyruvate kinase. (nih.gov)
  • Switching pyruvate kinase expression to the M1 (adult) isoform leads to reversal of the Warburg effect, as judged by reduced lactate production and increased oxygen consumption, and this correlates with a reduced ability to form tumours in nude mouse xenografts. (nih.gov)
  • PPDK
  • In the wild-type strain, the malate shunt accounts for about 33 ± 2% of the flux to pyruvate, with the remainder via the PPDK pathway. (osti.gov)
  • Causes
  • Pyruvate kinase M2 is more elevated in malignant pleural fluid than in plasma, and may be a useful biomarker to distinguish between malignant and non-malignant causes of pleural effusion. (clinicaltrials.gov)
  • rabbit
  • 1994. Structure of rabbit muscle pyruvate kinase complexed with Mn 2+ , K + , and pyruvate. (siumed.edu)
  • We have developed a novel enzymatic cycling method mediated by PK from rabbit muscle (RMPK) for the quantitative determination of pyruvate or PEP, which utilizes the reversibility of the reaction in the presence of an excess amount of ATP and IDP. (eurekalert.org)
  • enzymes
  • ADP-GK, one of the auxiliary enzymes, is inactive towards IDP and GDP, so the method should be applicable to other kinases that accept either IDP or GDP. (eurekalert.org)