Putative sp1 sp1 transcription factor. Medical search. Frequent questions

Another regulatory element, the cell cycle element/cell cycle genes homology region (CDE/CHR), located downstream of the start site is important for POLD1 transcription in G2/M phase by E2F1 and p21 proteins. (wikipedia.org)
In a genome-wide screen for putative tumor suppressor genes, the EBF3 locus on the human chromosome 10q26.3 was found to be deleted or methylated in 73% of the examined cases of brain tumors. (aacrjournals.org)
Upon binding of the estrogen ligand, the receptor is translocated to the nucleus and binds to the DNA of target genes that promote tumorigenesis but also regulate transcription of the receptor itself [ 1 ]. (oncotarget.com)
Insertions of TEs influence the transcriptional regulation of some genes as well as the termination of transcription. (ecancer.org)
Promoters of housekeeping genes contain built-in activating motifs for factors such as GABPA and YY1, which decrease the responsiveness of promoters to distal enhancers. (nature.com)
Candidate genes showing distinctive expression patterns in response to ethanol were clustered by pattern and further analyzed for related function, pathway membership and common transcription factor binding within and across clusters. (biomedcentral.com)
Interestingly, ID-associated genes exhibit promoter-localized MER41 LTRs that harbor TF binding sites (TFBSs) for not only STAT1 but also other immune TFs such as, in particular, NFKB1 (Nuclear Factor Kappa B Subunit 1) and STAT3 (Signal Transducer And Activator Of Transcription 3). (biorxiv.org)
Gene transcription is mainly regulated by transcription factors (TFs) that bind to specific DNA sequences (called motifs) located in the promoter regions of genes [ 1 ]. (biomedcentral.com)
PGCs express several pluripotency-related TFs such as NANOG, POU5F3 , and SOX2 , and their expression controls transcription of germness-related genes in these cells [ 11 , 29 ]. (biomedcentral.com)
Overexpression of ELF4 boosted CRC metastasis via transactivating its downstream target genes, fibroblast growth factor receptor 4 (FGFR4) and SRC proto-oncogene, non-receptor tyrosine kinase, SRC . (thno.org)
The objective of this work was the evaluation of muscle transcriptome profile in piglets of both genetic types, in order to identify genes, pathways and regulatory factors responsible for their phenotypic differences. (biomedcentral.com)
In this study, we employed a high-density single nucleotide polymorphism (SNP) chip to genotype 386 Nellore steers, a Bos indicus breed and, a Bayesian approach to identify genomic regions and putative candidate genes that could be involved with deposition and composition of IMF. (biomedcentral.com)
AhR regulates the transcription of diverse genes through binding to the xenobiotic-responsive element (XRE). (biomedcentral.com)
In this study, transcripts of putative POX genes were Olaparib structure also most abundant during early s. (igf-1rinhibitors.com)

P53 regulates POLD1 transcription by indirect p21-dependent activation of a p53-p21-DREAM-CDE/CHR pathway. (wikipedia.org)
Therefore, EBF3 regulates a transcriptional program underlying a putative tumor suppression pathway. (aacrjournals.org)
Furthermore, ectopically expressed NPAS3 in PC12 cells produced parallel responses for nuclear factor kappa-light-chain-enhancer of activated B cells [NF-κB (P65)] expression, which specifies that NPAS3 regulates VGF through the NF-κB signaling pathway. (frontiersin.org)
Using a composite gene-set analysis method, we combine the information of transcription factor binding sites, Gene Ontology or pathway gene sets and gene expression fold-change profiles for a variety of cell conditions. (biomedcentral.com)
It has also been demonstrated that STAT1 (Signal Transducer And Activator Of Transcription 1), a transcription factor (TF) crucially involved in the IFNγ pathway, binds consensus sequences that, in humans, are located with a high frequency in the LTRs (Long Terminal Repeats) of the MER41 family of primate-specific HERVs (Human Endogenous Retrovirus). (biorxiv.org)
However, the putative role of an IFNγ/STAT1/MER41 pathway in human cognition and/or behavior is still poorly documented. (biorxiv.org)
Pterostilbene is more potent than resveratrol in preventing azoxymethane (AOM)-induced colon tumorigenesis via activation of the NF-E2-related factor 2 (Nrf2)-mediated antioxidant signaling pathway. (ijpbp.com)

This segment lacked the typical TATA element, and possessed an initiator element and the putative binding sites for the transcription factor Sp1 and immediate-early protein IE180, a strong transactivator of PRV. (elsevierpure.com)
Examination of the promoter sequence indicated the presence of four Sp-1 consensus binding sequences and a putative initiator element surrounding the transcription initiation site. (elsevierpure.com)

One of the most important regulators of ER transcription is Sp1 (specificity protein 1) that is overexpressed in human ER positive (ER+) breast cancers [ 4 ]. (oncotarget.com)
TERT p duplications ( TERT p dup ) mainly affected the binding capacity of two transcription factors' families, i.e. the members of the E-twenty-six and the Specificity Protein/Krüppel-Like Factor groups. (biomedcentral.com)

One study has reported that the p53 tumor suppressor protein competes with Sp1 for binding to the POLD1 promoter. (wikipedia.org)
Members of this family include the signal-transducing G protein beta subunit, as well as other proteins that regulate signal transduction, transcription, pre-mRNA splicing, cytoskeletal organization, and vesicular fusion. (embl.de)
Neuronal PAS domain protein 3 (NPAS3) and VGF (VGF Nerve Growth Factor (NGF) Inducible) are important for neurogenesis and psychiatric disorders. (frontiersin.org)
Computer analysis reveals 10 consensus sequences for Sp1, six for activating protein 1 (AP-1), six for polyoma enhancer A3 (PEA3), 12 for AP-2 and five CCAAT boxes. (uea.ac.uk)
The +21/+58 region contains a putative binding site for the transcription factor leader-binding protein 1 (LBP-1). (uea.ac.uk)
For this purpose, WIN 55,212-2 was injected in pregnant wistar rats from gestation day 5 to 20 and a detailed analysis of the levels of the neurotrophin brain-derived neurotrophic factor (BDNF) as well as of the signaling molecules extracellular signal-regulated kinase (ERK)1/2 and alpha-calcium/calmodulin-dependent protein kinase II (alpha-CaMKII) was carried out in adult offspring. (researchgate.net)
A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)
Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2. (lookformedical.com)
We observe that each gene and its products have a unique set of DNA, RNA or protein motifs that encode a regulatory program to define the logical circuitry that guides the life cycle of these biomolecules, from transcription to degradation. (biomedcentral.com)
Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)

Several viral proteins have been shown to play an important role in tumor progression by modulating transcription factors. (biomedcentral.com)

this family of proteins is hereafter called EBF) are a group of DNA-binding transcription factors with the basic helix-loop-helix (bHLH) domain. (aacrjournals.org)
Second, Mutated enhancer box (E-box) of VGF promoter showed a significant response to this basic helix-loop-helix (bHLH) transcription factor, which suggested an indirect regulatory mechanism for controlling VGF expression by NPAS3. (frontiersin.org)

DNA meth-ylation represses transcription by interfering with transcription factor binding and indirectly by recruiting methyl-CpG-binding proteins and reducing chromatin remodeling activities ( 12 ). (spandidos-publications.com)
Chromatin immunoprecipitation assay, siRNA, and luciferase gene transcription assay in INS-1 cells were performed. (diabetesjournals.org)
Peak evaluation is complex, as gene expression regulation involves interactions between combinatorial transcription factor binding sites and chromatin states. (biomedcentral.com)
Chromatin assembly factor 1. (lookformedical.com)
This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. (lookformedical.com)

WD-repeat proteins are a large family found in all eukaryotes and are implicated in a variety of functions ranging from signal transduction and transcription regulation to cell cycle control and apoptosis. (embl.de)
3%). The organic phase is mainly formed by collagen type I fibers, which provide elasticity and flexibility to bone, but also non-collagenous proteins as well, such as cytokines, growth factors, and proteoglycans. (biomedcentral.com)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)

We observe several master transcription regulators (e.g. (biomedcentral.com)

Transient expression of a luciferase reporter gene under the control of serially deleted 5'-flanking sequences revealed that the 52-base pair region from - 326 to -275 upstream of the transcription initiation site includes a positive cisacting element(s) for the efficient expression of hTOP3 gene. (elsevierpure.com)
this is flanked by a series of upstream repressor elements for REST, Freud-1/CC2D1A and Freud-2/CC2D1B factors that not only restrict its expression to neurons, but may also regulate the level of expression of 5-HT1A receptors in various subsets of neurons, including serotonergic neurons. (biomedcentral.com)

T), are usually heterozygous, mutually exclusive, and produce an identical 11 bp 'CCCCTTCCGGG' sequence, resulting in the creation of de novo consensus binding motifs for E-twenty-six (ETS) transcription family members. (biomedcentral.com)

By analysing 5'-deletion mutants of the segment, a 48 bp segment (from nucleotide positions -65 to - 17), which possessed three Sp1 binding sites, was identified to be critical for the promoter activity. (elsevierpure.com)
Bioinformatic analysis of the 5' regulatory region has revealed that in addition to several ubiquitous transcription factors binding sites (SP1, AP1 and C/EBP) were found, several putative muscle-specific transcription factor binding sites (MyoD, MyoG and MEF2) were present in this region. (medwelljournals.com)
Computer-assisted searches of potential ERR-binding sites (ERR response element, ERRE, TNAAGGTCA) within the promoter region (-1 kb) was carried out by using the TESS database, which predicts transcription factor-binding sites. (biotech2012.org)
Analysis showed function-based networks, shared transcription factor binding sites and signaling pathways for members of the clusters. (biomedcentral.com)

Therefore, we investigated the mechanism that regulates transcription of the chicken NANOG ( cNANOG) gene in PGCs and ESCs. (biomedcentral.com)

In vitro studies of INS-1 cells confirmed that PPAR-γ binds to the putative PPRE sequence and regulates GIP-R transcription. (diabetesjournals.org)

Furthermore, a continuous decrease of in vivo bound transcription initiation factors to the CD3 γ promoter was observed after silencing of the viral genome. (biomedcentral.com)
The presence of a single transcription initiation site was suggested by primer extension analysis. (elsevierpure.com)

The transcription of this GC box-containing promoter is regulated by Sp1 and Sp1-related transcription factors such as Sp3, with their binding mediated via 11-bp repeat binding sequences. (wikipedia.org)

Finally, we describe a novel mechanism, how BCL9-2 regulates ER transcription by interaction with Sp1 through the proximal ESR1 gene promoter. (oncotarget.com)
BRG1 was pre viously shown to manage MMP2 expression in SW13 cells by a transcriptional mechanism that entails SP1. (topoisomerasepathway.com)
Our information indicate that BRG1 activates MMP2 expression in melanoma cells by a equivalent mechanism involving co activation of SP1 mediated transcription. (topoisomerasepathway.com)

Open in a separate window Number 4 ERR interacts with p65 to regulate transcription. (biotech2012.org)

The aim of this study was to test for the molecular and functional expression of the non-gastric H + /K + ATPase ATP12A/ATP1AL1 in unstimulated and butyrate-stimulated (1 and 10 mM) human myelomonocytic HL-60 cells, to unravel its potential role as putative apoptosis-counteracting ion transporter as well as to test for the effect of the H + /K + ATPase inhibitor SCH28080 in apoptosis. (karger.com)
An ectotherm's thermal tolerance is determined by both fixed genetic factors and plastic changes in behavior, morphology, physiology, and gene expression. (frontiersin.org)
Cotransfection of Vero cells with the mutant constructs and an IE180 expression plasmid resulted in transactivation of only those constructs in which the Sp1 sites were present. (elsevierpure.com)
The present study investigated the methylation of CpG sites in the cyclooxygenase (COX)‑2 promoter via nuclear factor (NF)‑κB transcriptional regulation and elucidated its effect on the COX‑2 transcriptional expression in a ketamine‑induced ulcerative cystitis (KIC) animal model. (spandidos-publications.com)
We propose an approach for mining context-specific transcription networks from a large collection of gene expression fold-change profiles and composite gene-set information. (biomedcentral.com)
Krüppel-like factor 15 activates hepatitis B virus gene expression and replication Hepatology. (usc.edu)
As part of civil aviation safety research into aeromedical factors impacting flight safety, we have undertaken a time course study on the effects of acute ethanol exposure to the legal intoxication level of blood alcohol concentration (BAC), 0.08 g/dL (0.08% wt/vol), on human gene expression in non-chronic users. (biomedcentral.com)
Fibroblast growth factor 19 (FGF19) upregulated ELF4 expression through the ERK1/2/SP1 axis. (thno.org)
Regulatory factors (RF) potentially involved in the expression differences were identified by calculating the regulatory impact factors . (biomedcentral.com)
TSSP-TCM software and PlantCARE analysis showed a TATA-box core element, a CAAT-box, root specific expression element, light regulation elements including chs-CMA1a, Box I, and Sp1 and other cis-acting elements in the sequence. (biomedcentral.com)
Real-time Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) analysis of EcGS1b gene also showed higher expression in the roots of E.crassipes than in stems and leaves. (biomedcentral.com)
However, BRG1 inhibited the expression of integrin b3, which is also regulated by SP1. (topoisomerasepathway.com)

The POLD1 promoter contains an E2F-like sequence located near the major transcription start site. (wikipedia.org)

As demonstrated in Number ?Number4D,4D, ERR induced powerful luciferase manifestation in pGL3-promoter to regulate its transcription. (biotech2012.org)
The luciferase assay confirmed that TERT p dup behaved as gain-of-function mutations causing a 2,3-2,5 fold increase of TERT transcription. (biomedcentral.com)

EBF3 bound directly to p21 cip1/waf1 promoter and regulated transcription from both p21 cip1/waf1 and p27 kip1 promoters in reporter assays. (aacrjournals.org)
Promoters can initiate the transcription of its downstream gene. (biomedcentral.com)

A separate set of allele-specific factors, including Deaf1, Hes1 and Hes5 repress at the HTR1A C(-1019)G (rs6295) polymorphism in serotonergic neurons in culture, as well as in vivo . (biomedcentral.com)

It can specifically drive the transcription of its downstream gene in root. (biomedcentral.com)

These results indicate that the EP0 gene may be transcribed from the TATA-less promoter that responds to Sp1. (elsevierpure.com)

Moreover, in the ketamine group, the level of Ten‑Eleven‑Translocation methylcytosine dioxygenase for demethylation as determined by reverse transcription‑quantitative PCR assay was increased in comparison with the control group, but that was not the case for the level of DNA methyltransferases for methylation. (spandidos-publications.com)

Furthermore, modification of genetic susceptibility by environmental factors, both endogenous and exogenous, may alter the degree of penetrance. (hindawi.com)
Although the current generation of dCas9-based transcription activators is able to achieve up-regulation of some endogenous loci, the magnitude of transcriptional up-regulation achieved by individual single-guide RNAs (sgRNAs) 12 typically ranges from low to ineffective 8 - 11 . (cdc.gov)

In many of these cases, a single exon is involved, and its transcription yields a variant mRNA [ 5 ]. (ecancer.org)
RNase protection identifies six transcription start points, making exon 1 up to 48 bp in length. (uea.ac.uk)

Combining this work and published literature, a potential network composed by NPAS3, NF-κB, Brain-Derived Neurotrophic Factor (BDNF), NGF and VGF, was proposed. (frontiersin.org)

Regulation of the NANOG gene by TFs, epigenetic factors, and autoregulatory factors is well characterized in ESCs, and transcriptional regulation of NANOG is well established in these cells. (biomedcentral.com)

We investigated the role of PPARγ in GIP-R transcription. (diabetesjournals.org)
There is strong evidence that inherited genetic factors (mutations plus single nucleotide polymorphisms) can play a major role in breast cancer susceptibility [ 1 ]. (hindawi.com)
Furthermore, small interfering RNA-mediated knockdown demonstrated that POU5F3 , SOX2 , and CEBP played a role in cell type-specific transcription of cNANOG . (biomedcentral.com)

Several HPV types, especially high-risk types (HPV-HR), mediate squamous intraepithelial lesion (SIL) development that may progress to cervical cancer through several mechanisms such as keratinocytes malignant transformation, however many other factors contribute to the disease progression, such as tobacco use, long-duration oral contraceptive use and multiparity [ 3 ]. (biomedcentral.com)
Here, we investigated the function of E74-like factor 4 (ELF4), an ETS family member, in facilitating CRC progression. (thno.org)

Human POLD1/p125 has a putative nuclear localization signal at the N-terminal end (residues 4-19). (wikipedia.org)
We then collected all the significant patterns and constructed a database of context-specific transcription networks for human ( REGNET ). (biomedcentral.com)
Together, this systematic assessment of enhancer-promoter compatibility suggests a multiplicative model tuned by enhancer and promoter class to control gene transcription in the human genome. (nature.com)

With a series of deletion/point mutants, we identified CaM antagonist-responsive region in the putative Sp1-binding domain between -295 to -300 base pairs of DR5 gene. (oncotarget.com)
On the basis of gel mobility shift and supershift assays, we demonstrated that both YY1 and USF1 transcription factors can bind to the 52-base pair region. (elsevierpure.com)

Transient transfection experiments showed that the CD3 γ promoter was still active in CD3 - HTLV-I infected cells demonstrating that adequate amounts of the required transcription factors were available. (biomedcentral.com)
We previously showed that peroxisome proliferator-activated receptor (PPAR)-γ in β-cells regulates pdx-1 transcription through a functional PPAR response element (PPRE). (diabetesjournals.org)
Mesenchymal stem cells (MSCs), which are activated by secreted, active transforming growth factor beta 1 (TGFβ) to migrate to bone-resorptive sites and differentiate into osteoblasts (see below). (biomedcentral.com)
Pre-osteoblasts, a heterogeneous population of cells, including those transitioning from MSCs to mature osteoblasts, which express the transcription factor runt related transcription factor 2 (RUNX2) , a key player in the osteogenesis process. (biomedcentral.com)
NANOG is a core transcription factor (TF) in embryonic stem cells (ESCs) and primordial germ cells (PGCs). (biomedcentral.com)

As a result, context-specific roles of transcription factors as well as their functional targets are readily explored. (biomedcentral.com)
REGNET is a useful tool for exploring the ternary relationships among the transcription factors, their functional targets and the corresponding cell conditions. (biomedcentral.com)

We first identified the transcription start site of cNANOG by 5′-rapid amplification of cDNA ends PCR analysis. (biomedcentral.com)

Transcription factors and regulatory mechanisms are proposed for these altered biological functions. (biomedcentral.com)

One main reason for the difficulty is the dynamic nature of the networks: Transcription factors (TFs) not only regulate different targets depending on the cell conditions, but their effects on these targets can also change. (biomedcentral.com)

e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens. (lookformedical.com)

As a consequence, bone aging is the main risk factor for primary osteoporosis, characterized by a reduction in bone mineral density, predisposing the elderly population to an increased risk of fractures. (biomedcentral.com)

T. In silico analysis predicted the formation of 119 and 108 new transcription factor's recognition sites for TERT p c.1-100_1-79dup and TERT p c.1-110_1-89, respectively. (biomedcentral.com)

The present findings revealed that there was a hypomethylation pattern of the COX‑2 promoter in association with the level of COX‑2 transcription in KIC. (spandidos-publications.com)

Anatomy15

Organisms8

Chemicals and Drugs137

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes70

Disciplines and Occupations1

Information Science4

Genes14

Pathway7

Initiator element2

Specificity protein2

Protein10

Tumor1

Basic helix2

Chromatin5

Proteins4

Regulators1

Upstream2

Consensus1

Binding sites4

Regulates Transcription1

Binds1

Initiation2

Sequences1

Mechanism3

Regulate transcription1

Expression12

Sequence1

Luciferase2

Promoters2

Vivo1

Downstream1

TATA1

Assay1

Endogenous2

Exon2

BDNF1

Transcriptional regulation1

Role3

Progression2

Human3

Region2

Cells5

Functional2

Site1

Mechanisms1

Targets1

Tissue1

Reduction1

Recognition1

Present1