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GelsChromatography, High Pressure LiquidChromatography, AffinityChromatography, GelChromatographyChromatography, GasChromatography, Ion ExchangeChromatography, Thin LayerChromatography, LiquidComplex MixturesChromatography, DEAE-CelluloseMolecular WeightMolecular Sequence DataKineticsElectrophoresis, Polyacrylamide GelElectrophoresis, Gel, Two-DimensionalMass SpectrometryAmino Acid SequenceChromatography, AgaroseGas Chromatography-Mass SpectrometryHydrogen-Ion ConcentrationAmino AcidsChromatography, Reverse-PhaseChromatography, PaperSubstrate SpecificityElectrophoresis, Gel, Pulsed-FieldElectrophoresis, Agar GelSepharoseIsoelectric FocusingEscherichia coliCattleTemperatureBase SequenceSpectrophotometry, UltravioletTandem Mass SpectrometryChemistrySpectrometry, Mass, Electrospray IonizationChemical PhenomenaMagnetic Resonance SpectroscopyRabbitsProtein BindingPeptide FragmentsChemical FractionationMethodsLiverDrug StabilityTrypsinHydrolysisPeptidesCarbohydratesElectrophoresisRecombinant ProteinsIndicators and ReagentsOligosaccharidesSolubilityCarbohydrate SequenceStereoisomerismCountercurrent DistributionBinding SitesBacterial ProteinsCloning, MolecularChromatography, Micellar Electrokinetic CapillaryReproducibility of ResultsHot TemperatureHydroxyapatitesCell LineSolventsSwineDNAMacromolecular SubstancesSpecies SpecificityProteinsCentrifugation, Density GradientIsoelectric PointMolecular StructureChemical PrecipitationTime FactorsPolysaccharidesBiological AssayUltracentrifugationPhospholipidsFatty AcidsReference StandardsCalibrationMicrochemistryPlant ExtractsRadioimmunoassayOxidation-ReductionAcetonitrilesIsoenzymesSpectrophotometryPhysicochemical PhenomenaImmunodiffusionAmmonium SulfateProteomicsCells, CulturedCarbohydrate ConformationChemistry, PhysicalGlycoproteinsMethanol
Silica gel platesHPLCSeparate mixturesResiduePreparativeColumnEthyl acetateReaction mixture was dilutedLiquidSolventsSeparationCompoundsCompoundRoom temperatureRegardElectrophoresisCharged resinWashed with saturatedInletMmolResearchersFlashFlaskMethodSynthesisMassNoteSolutionThinFullyStandardMaterialHoursFactorsRemoveLeads
Silica gel plates1
  • The reactions were monitored by thin layer chromatography using silica gel plates and detected by UV chamber and iodine as visualizing agent. (ijpsonline.com)
HPLC7
  • Since their inception in the 1970s, superficially porous particles (SPPs) have offered good performance and are indispensable in high-speed high performance liquid chromatography (HPLC) (1). (chromatographyonline.com)
  • Automated flash chromatography systems include components normally found on more expensive HPLC systems such as a gradient pump, sample injection ports, a UV detector and a fraction collector to collect the eluent. (ijpsr.com)
  • In the present study, cordycepin was prepared by preparative high‑performance liquid chromatography (prep-HPLC) and the purity obtained was 99.6%, indicating that this technique may be useful for the large‑scale isolation of cordycepin in the future. (spandidos-publications.com)
  • C) HPLC chromatogram of purified cordycepin sample compared with the standard. (spandidos-publications.com)
  • HPLC, high-performance liquid chromatography. (spandidos-publications.com)
  • Preparative high performance liquid chromatography (prep-HPLC) can shorten the production cycle, has relatively low operating costs, and is simple to carry out. (spandidos-publications.com)
  • Prep-HPLC can be used to provide purified samples on a kilogram, or even ton, scale. (spandidos-publications.com)
Separate mixtures2
  • Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. (lookformedical.com)
  • Flash chromatography is a technique used to separate mixtures of molecules into their individual constituents, frequently used in the drug discovery process 1 . (ijpsr.com)
Residue4
  • The residue was purified by column chromatography on silica gel (methanol-dichloro-methanegradientelution)toaffordGlcNGc(0.8g)in15%yield overall. (glassbox.tv)
  • The crude residue was purified by silica gel flash chromatography. (sigmaaldrich.com)
  • The solvent was removed under reduced pressure to give a crude residue which was purified by flash silica gel chromatography (eluent: ethyl acetate/petroleum ether (v:v= 1: 2)) to give AU (yield: 98percent) as a colorless oil. (ambeed.com)
  • The combined organic layers were dried over sodium sulfate and the solvent concentrated in vacuo and the residue was purified by chromatography on silica gel to afford the corresponding products. (sioc-journal.cn)
Preparative4
  • In previous days, Column chromatography was used in many laboratories for preparative purposes as well as for reaction control in organic synthesis. (ijpsr.com)
  • This leads to the development of novel preparative liquid chromatography in which mobile phase flows down by positive air pressure called as Flash chromatography. (ijpsr.com)
  • It is a simple, fast and economical approach to preparative Liquid chromatography .This review try to focus on principle, various components, general procedure, advantages and application of Flash chromatography. (ijpsr.com)
  • Column chromatography has found its place in many laboratories for preparative purposes as well as for reaction control in organic syntheses. (ijpsr.com)
Column22
  • The resulting reaction mixture is filtered to remove dicyclohexylurea, evaporated to dryness, then purified by column chromatography on silica gel. (wikipedia.org)
  • After the respective times of reaction described in Table 1 , crude products were isolated, filtered over column chromatography of silica gel, and then analysed by 1 H NMR. (hindawi.com)
  • These two products may be separated by means of column chromatography. (scirp.org)
  • The purification is carried out by silica gel column chromatography to give Boc-ΔTyr(Cl 2 Bzl)-Gly-NH 2 (317 mg, 85% yield). (tcichemicals.com)
  • The preliminary isolation of C. zeyheri active compounds was carried out using chromatographic techniques which include sephadex gel column chromatography, silica gel column chromatography and thin-layer chromatography (TLC). (biomedcentral.com)
  • Purification the reaction mixture with desalting column. (aatbio.com)
  • Purify the conjugate by column chromatography with PBS buffer or other buffer of choice. (aatbio.com)
  • Note If the reaction volume is ~100 µL, spin column (Cat# 60500) can be used to purify. (aatbio.com)
  • 1 mL, we recommend packing a column using Sephadex® G-25 media or Bio-Gel® P-6DG Gel with an appropriate volume to purify the reaction mixture. (aatbio.com)
  • Column chromatography is an extremely time consuming stage in any lab and can quickly become the bottleneck for any process lab. (ijpsr.com)
  • Column chromatography is separated into two categories, depending on how the solvent flows down the column. (ijpsr.com)
  • If the solvent is allowed to flow down the column by gravity, or percolation, it is called gravity column chromatography. (ijpsr.com)
  • If the solvent is forced down the column by positive air pressure, it is called flash chromatography, a "state of the art" method currently used in organic chemistry research laboratories 1 . (ijpsr.com)
  • In traditional column chromatography a sample to be purified is placed on the top of a column containing some solid support, often silica gel. (ijpsr.com)
  • The rest of the column is then filled with a solvent (or mixture of solvents) which then runs through the solid support under the force of gravity. (ijpsr.com)
  • In flash chromatography however air pressure is used to speed up the flow of solvent, dramatically decreasing the time needed to purify the sample, therefore making the column and running the separation could take less than 10-15 minutes. (ijpsr.com)
  • Flash chromatography is basically an air pressure driven hybrid of medium pressure and shorter column chromatography which has been optimized for particularly rapid separation. (ijpsr.com)
  • Flash chromatography differs from the conventional technique in two ways: first, slightly smaller silica gel particles (250-400 mesh) are used, and second, due to restricted flow of solvent caused by the small gel particles, pressurized gas ( ca. 10-15 psi) is used to drive the solvent through the column of stationary phase. (ijpsr.com)
  • various drawbacks have been identified in the purification techniques of ion resin adsorption ( 12 , 13 ), silica gel column chromatography ( 14 ) and supercritical extraction ( 15 ). (spandidos-publications.com)
  • Silica gel 60 (Merck, 70-230 mesh and 230-400 mesh) was used for column chromatography. (richberry.ph)
  • Confirmation of GC analysis is typically accomplished using gas chromatography with mass spectroscopy (GC-MS). The preparation of samples for GC analysis typically involves sample extraction with an organic solvent (e.g., hexane or diethyl ether) and purification of the extract (e.g., by column chromatography or further extraction). (cdc.gov)
  • The extracted DCA and MCA was then derivatized by ethylation or methylation and purified by column chromatography on silica gel using an ethyl acetate/benzene eluent. (cdc.gov)
Ethyl acetate1
  • The reaction mixture was stirred at room temperature for 10 minutes and then diluted with ethyl acetate or ether and washed with either 0.5 N KHSO 4 or 0.5 N HCl (2x) and brine (1x). (sigmaaldrich.com)
Reaction mixture was diluted2
  • The reaction mixture was diluted with hexanes (30 ml), filtered through a pad of Celite, and concentrated at reduced pressure. (glassbox.tv)
  • The reaction mixture was diluted with saturated aqueous NaHCC>3 (90 mL) and extracted with EtOAc (400 mL). (ambeed.com)
Liquid7
  • The resultant liquid is a mixture of dibenzyl ketone and minor impurities. (wikipedia.org)
  • Almost 50 years later, the C18 phase is still the reigning champion in the reversed-phase liquid chromatography (RPLC) arena, and many chromatography companies are still bonding using the same or a very similar synthetic approach. (chromatographyonline.com)
  • According to the United States Pharmacopeia ( USP ), there are 858 C18 liquid chromatography phases registered under code L1 (octadecylsilane chemically bonded to porous silica or ceramic microplates, 1.5 to 10 μm in diameter, or a monolithic rod) (3). (chromatographyonline.com)
  • Eleven fractions of IC 70 BA isomers were separated by high-performance liquid chromatography. (beilstein-journals.org)
  • Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)
  • Moreover, caffeic acid and p-Coumaric acid were confirmed using ultra-pressure liquid chromatography and the amount was 194.43 and 0.9272 mg/kg, respectively. (bvsalud.org)
  • MPLC - Medium pressure liquid chromatography (MPLC) systems which operate above 150 psi. (ijpsr.com)
Solvents1
Separation1
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
Compounds3
Compound4
  • The title compound (E)-1-{4-[bis(4-methoxyphenyl)methyl]piperazin-1-yl}-3-(4-chloro- phenyl)prop-2-en-1-one (C 28 H 29 ClN 2 O 3 , Mr = 476.98) (5) was synthesized and studied by the single crystal X-ray diffraction method. (scielo.org.za)
  • 13 The title compound (E)-1-{4-[bis(4-methoxyphenyl)methyl]piperazin-1-yl}-3-(4-chlorophenyl)prop-2-en-1-one 5 was obtained as follows:thionylchloride (2mL) and piperadine (1 drop) were added to a stirred solution of (E)-3-(4-chlorophenyl)acrylic acid (4,4 mmol) in dichloromethane (15 mL) at room temperature. (scielo.org.za)
  • The software controlling an automated system coordinate the components, allow a user to only collect the factions that contain their target compound (assuming they are detectable on the system's detector) and help the user to find the resulting purified material within the fraction collector. (ijpsr.com)
  • The partially purified compound after ion-exchange chromatography was found to be thermoresistant and stable under wide range of pH. (researchsquare.com)
Room temperature3
  • The reaction mixture is stirred at room temperature for an additional 2 h ( Note 3 ) at which point it is transferred to a 500-mL separatory funnel using diethyl ether (100 mL) to rinse the flask. (orgsyn.org)
  • Typical Procedure: To a solution of Boc-ΔTyr(Cl 2 Bzl)-OH (307 mg, 0.7 mmol), glycinamide hydrochloride (77.4 mg, 0.7 mmol) and Et 3 N (0.1 mL, 0.7 mmol) in DMF (7 mL) is added EDC·HCl (148 mg. 0.77 mmol) and HOBt (129 mg, 0.84 mmol) at -10°C. The reaction mixture is stirred for 2 h at 0°C. and overnight at room temperature. (tcichemicals.com)
  • The mixture was then stirred at room temperature overnight with the flask open to the atmosphere. (ambeed.com)
Regard1
  • Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (lookformedical.com)
Electrophoresis2
  • Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. (lookformedical.com)
  • This network is based on characterization of whole bacterial genomes by using macrorestriction digestion patterns that are separated by pulsed-field gel electrophoresis (PFGE), a technique that has emerged as a common standard for subtyping EHEC O157 isolates ( 6 ). (cdc.gov)
Charged resin1
Washed with saturated1
  • The mixture is washed with saturated NH 4 Cl (1 x 100 mL), saturated NaHCO 3 (1 x 100 mL), and brine (1 x 100 mL). (orgsyn.org)
Inlet1
Mmol4
  • The reaction mixture was cooled to 0 °C, then EDC·HCl (515 mg, 2.71 mmol) was added. (tcichemicals.com)
  • Then the Me 2 S0 4 (1.1 g, 8.48 mmol) was added to the reaction mixture. (ambeed.com)
  • A mixture of 1-(2-chloro-phen-yl)-3,3-bis-(methyl-sulfan-yl)prop- 2-en-1-one, 1 (2.0 mmol, 1 equiv. (iucr.org)
  • Step 1: Synthesis of tert-butyl 5-hydroxypentylcarbamate (AU)[0558] To a stirred solution of 5-aminopentan-l-ol (AT, 3.1 g, 30.05 mmol) in dichloromethane (30 mL) was added di-tert-butyl dicarbonate (6.56 g, 30.06 mmol) at 0 °C. The resulting mixture was then stirred at rt for 4h. (ambeed.com)
Researchers2
  • In this recent review published in the journal Gels, iBB researchers Filipe Miguel (MSc student in Biotechnology), Frederico Barbosa (PhD student in Bioengineering), Prof. Frederico Ferreira and Dr. João Silva highlight the most common conductive materials combined with hydrogels and their diverse applications, and discuss the current state of research on the development of electrically conductive hydrogels for cartilage tissue engineering. (scoop.it)
  • The growing concerns about water pollution have prompted researchers to explore new materials for remediating and purifying it. (bvsalud.org)
Flash2
Flask2
  • The mixture is transferred to a 500-mL separatory funnel using diethyl ether (20 mL) to rinse the flask. (orgsyn.org)
  • The [ 11 C]1-(4-(((6-amino-5-(4-phenoxyphenyl)pyrimidin-4-yl)amino)methyl)piperidin-1-yl)prop-2-en-1-one ([ 11 C]evobrutinib) was isolated between the 15.5 and 18 min mark of the chromatogram and this sample was collected into a dilution flask that contained 50 ml of a 2 mg/ml sodium ascorbate aqueous solution. (drugapprovalsint.com)
Method1
  • One method is where phenylacetic acid is reacted with acetic anhydride and anhydrous potassium acetate and refluxed for two hours at 140−150 °C. The mixture is distilled slowly so that the distillate is mostly acetic acid. (wikipedia.org)
Synthesis1
  • 7 In this article, we present our results on the synthesis, crystal structure and anti-ischaemic activity of new cinnamide derivative of (E)-1-{4-[bis(4-methoxyphenyl)methyl]piperazin-1- yl}-3-(4-chlorophenyl)prop-2- en-1-one (5). (scielo.org.za)
Mass1
Note1
  • Stability of serum obtained with tubes containing separating gel: 48 hours at 2-8°C (note the data provided by the tube manufacturer). (cdc.gov)
Solution2
  • To quench the reaction methanol was carefully added to the stirred reaction mixture, followed by the slow addition of 1N-HCl solution. (glassbox.tv)
  • The intensity of the sweetness induced by 0.02 mol citric acid after 0.4 mola purified miraculin solution was held in the mouth is equivalent to that of about 0.3 mol sucrose. (richberry.ph)
Thin2
Fully2
  • The fully hydrated form of silica gel has distinct properties and is referred to as SILICIC ACID. (lookformedical.com)
  • Hybridized DNA CodeSet complexes had been purified applying the fully automated nCounter prep station, and reporters were counted applying the nCounter digital analyzer. (mirnaarray.com)
Standard1
  • Serum collected using standard sampling tubes or tubes containing separating gel. (cdc.gov)
Material2
  • Following an initial work on the isolation of a single geometric isomer from an indene-C 70 bisadduct (IC 70 BA) mixture, we report the full fractionation and identification of the bisadduct species in the material. (beilstein-journals.org)
  • The IC 70 BA material used in most reports consisted of a mixture of isomers [7-9] . (beilstein-journals.org)
Hours2
  • The reaction mixture was stirred at RT for 3.5 hours and was subsequently diluted with water (500 mL). (ambeed.com)
  • The reaction mixture was stirred at ambient temperature for 6 hours. (scielo.org.za)
Factors1
  • Select when the contaminant and concentration of the contaminant are known and the respiratory protection criteria factors for using Air Purifying Respirators (APR) or Powered Air Purifying Respirators (PAPR) are met. (cdc.gov)
Remove1
  • Author Manuscript and RNA repair protein AlkB-derived enzyme mixture was first used to remove methylations at the Watson-Crick face. (cdc.gov)
Leads1
  • Heating the mixture above 200−205 °C leads to resinification with a decrease in the yield of the ketone. (wikipedia.org)