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  • reagents
  • This position is responsible for the production and development of proteins and molecular biology reagents, with a focus on molecular biology and next generation sequencing (NGS) applications. (genomeweb.com)
  • A growing demand for protein purification reagents, in line with an increasing number of protein-based research studies and diagnostic tests is expected to contribute to the growth of the consumables market during the forecast period. (marketsandmarkets.com)
  • buffer
  • 4 to ppt DNA (or using PEI, protamine sulfate, etc...) 2) Trying Exclusion on Q-media first then running through S-media 3) Dilution of detergent to levels below CMC 4) Alternative buffer systems 5) Checking pI of the protein (sorry if I excluded any others, but I took them all into consideration! (bio.net)
  • The wells remain liquid-tight during incubation of the gel matrix with buffer, during which time the protein diffuses from the gel into the buffer. (bio-medicine.org)
  • if still ur protein is in inclusion body add some amount (2mM) 2-mercaptoethanol ( some times its work) in ur buffer. (protocol-online.org)
  • If you are worried about the binding of non-specific proteins then equlibrate the column with buffer + imidazole prior to binding your protein. (protocol-online.org)
  • precipitation
  • Uppa-PROTEIN-Concentrater Kit, Micro (Thomas No. C994C97) Protein solution as dilute as 1 ng/ml can be quantitatively concentrated by using the Universal Protein Precipitation reagent followed by solubilization and centrifugation through a Gel filtration spin column (provided). (thomassci.com)
  • assay
  • The protein-binding conditions are those for gel retardation assay. (bio.net)
  • you can download the protein assay technical handbook from thermo-pierce to help guide you in selecting an appropriate assay. (protocol-online.org)
  • assays
  • The simple purification system is also applicable to pulldown experiments and kinetic activity or binding assays, such as surface plasmon resonance. (newswise.com)
  • It is assumed that you are familiar with the theoretical background to the most common separation techniques, enzyme assays etc. and that you understand the concept of the isoelectric point of proteins. (appadvice.com)
  • dialysis
  • In Yuling's case, I don't know if it would work - the protein should probably stay soluble in the NaI since its a denaturant, but I guess there's a pretty good chance it'll come out of solution during dialysis - maybe other people's suggestions will help with that. (bio.net)
  • nucleic
  • and two nucleic acid binding proteins, the multisubunit condensin protein complex of Salmonella typhimurium that folds and compacts cellular DNA, and the human remodeling and spacing factor complex, RSF, which is implicated in mediating nucleosome assembly. (newswise.com)
  • cytoplasmic
  • FOCUS™ Cytoplasmic/Nuclear Protein Extraction (Thomas No. C994C79) Fractionation method is based on gentle lysis of the sample followed by centrifugation to collect and separate nuclei from the rest of the cytoplasmic proteins. (thomassci.com)
  • yields
  • Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction using these cell-free kits. (fishersci.com)
  • We use optimized conditions throughout the workflow, enabling us to routinely and efficiently obtain high yields of purified proteins. (thermofisher.com)
  • Principles
  • This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. (ecampus.com)
  • beads
  • Magnetic beads and resins are available in multiple formats to accommodate a variety of needs, from high-throughput screening, batch, pilot, and process purification. (thermofisher.com)
  • columns
  • We offer a wide range of disposable plastic columns for small-scale purification needs, both in spin- and gravity-based formats: Pierce™ Spin Cups and Columns. (thermofisher.com)
  • extract
  • However, I did manage to get a good separation of proteins by running my cell extract on a Q-column (I used a Bio-Rad Econo-Q-Column). (bio.net)
  • applications
  • On the other hand, ion exchange and hydrophobic charge interaction chromatography (HCIC) are expected to maintain a high customer base, particularly for preparative or large-scale applications, as users often employ multiple technologies in the protein purification process for their purification needs. (marketsandmarkets.com)
  • Protease
  • Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors. (fishersci.com)
  • separations
  • With the many recent advances in technology, simple spectrometric detection is no longer the only option for separating proteins, and the authors treat in full detail all the newer methods for these separations. (springer.com)