Mutant Chimeric ProteinsRecombinant Fusion ProteinsMolecular Sequence DataAmino Acid SequenceEscherichia coliMolecular WeightElectrophoresis, Polyacrylamide GelBase SequenceChromatography, AffinityCloning, MolecularKineticsCell LineChromatography, Ion ExchangeSubstrate SpecificityBacterial ProteinsProtein Structure, TertiaryChromatography, GelSequence Homology, Amino AcidPlasmidsMutant ProteinsMutagenesis, Site-DirectedProtein BindingBinding SitesChimeraMutationTransfectionDNA-Binding ProteinsHydrogen-Ion ConcentrationDNA PrimersTemperatureMembrane ProteinsChromatographyAmino AcidsGenetic Complementation TestRecombinant ProteinsTranscription FactorsPhenotypeCell MembraneSequence AlignmentStructure-Activity RelationshipSequence DeletionCarrier ProteinsModels, Molecular