The GH19 Engineering Database: sequence diversity, substrate scope, and evolution in glycoside hydrolase family 19. (uni-stuttgart.de)
Recombinant3
Purification and Appearance of recombinant Sse. (lacbiosafety.org)
Plant-produced recombinant LipA is active towards p -nitrophenol esters of various carbon chain lengths with peak activity on medium-chain fatty acid (C8). (biomedcentral.com)
So-called epitope affinity tags bound to the desired protein enable selective purification of recombinant proteins from the cell extract. (biomers.net)
Protein7
These protein consist of hydrolases that degrade protein and nucleic Procyclidine HCl acids (25, 33). (lacbiosafety.org)
Synthetic peptide taken within amino acid region 200-250 on mouse a/b hydrolase II protein. (thermofisher.com)
This gene encodes a protein containing an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. (thermofisher.com)
The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. (thermofisher.com)
1. Overview of Affinty Tags for Protein Purification. (biomers.net)
A notable representative among protein tags is the HaloTag®, which is widely used in protein analysis (purification, trafficking, localisation, protein-protein interactions and multiplex imaging using antibodies or GFP). (biomers.net)
In this study, we confirmed that both GrmH and GrmL, which were shown to constitute a protein complex by a co-purification experiment, were required to catalyze the dehydration, epimerization, and proteolytic cleavage of a precursor peptide GrmA by in vivo experiments. (hokudai.ac.jp)
Syntheses2
The optimised conditions for sugar fatty acid ester syntheses are 48 h at 2:1 of molar ratio of lactose sugar to capric acid at 55 °C. Furthermore, a high operational stability with half-lives of over 13 and 10 runs was achieved for NER-CRL and Amino-CRL, respectively, indicating the efficiency of the immobilisation process. (researchgate.net)
A specific overview regarding synthetic methodologies and properties of 6′-lactose-based surfactants is presented herein, particularly all the synthetic approaches to this class of lactose esters, such as enzymatic and traditional organic syntheses. (researchgate.net)
Enzymes2
The carboxylic ester hydrolases certainly are a different band of enzymes that may divide the carboxylic acidity ester connection in carboxylic esters, triglycerides, phospholipids, and/or acetylcholine (34) and therefore may play essential roles in tissues invasion and nutritional utilization by bacterias. (lacbiosafety.org)
Enzymes that catalyze the hydrolysis of ester bonds within RNA. (bvsalud.org)
Acids1
The AOR/AdhA pathway also converts exogenously added aliphatic and aromatic carboxylic acids to the corresponding alcohol using glucose, pyruvate, and/or hydrogen as the source of reductant. (brenda-enzymes.info)
Bonds1
This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. (wikipedia.org)
Enzyme3
Enzyme immobilisation technology is an effective means to improve sugar ester production through the employment of biocatalysts. (researchgate.net)
When the ligand is bound to the HaloTag® enzyme, the halogen group of the linker is cleaved by a nucleophilic displacement mechanism and released so that a covalent and irreversible ester bond can be formed between the haloalkane and the amino acid residue aspartate in the enzyme. (biomers.net)
These studies have been dedicated to a variety of macromolecular systems with different structural complexities and have been carried out by combining X-ray crystallographic methods with several other techniques, including enzyme expression and purification, enzyme functional characterization, molecular modelling, kinetic and spectroscopic analysis. (cnr.it)
Synthesis1
Extending this work, immobilised lipases have novel capabilities in the synthesis of sugar esters. (researchgate.net)
Active1
The HaloTag® is a 34 kDa-sized haloalkane dehalogenase, which acts as a hydrolase and has a modified active site. (biomers.net)
Properties1
Biological applications with a focus on permeability enhancing, antimicrobial activity, and antibiofilm properties of 6′-lactose-based esters are also reported. (researchgate.net)
Family of hydrolases2
This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. (wikipedia.org)
Lipases (triacylglycerol acylhydrolase, EC 3.1.1.3) are part of the family of hydrolases that act on carboxylic ester bonds. (hindawi.com)
Isolation1
the characterization of these antibiotics was made on the basis of: antifungal activity against p. chrysogenum, isolation and purification of the active substance, quantitative analysis of alpha-aminoacids and identification of the liposoluble dipeptide obtained by partial hydrolysis. (liverpool.ac.uk)
Activity1
Our initial studies on ground corncob bedding extracts led to the purification of two peaks of mitogenic activity on reverse-phase high-performance liquid chromatography (HPLC). (nih.gov)