• Octameric enolase from the hyperthermophilic bacterium Thermotoga maritima: purification, characterization, and image processing. (uni-regensburg.de)
  • Purification and characterization of human liver microsomal cytochrome P-450 2A6. (aspetjournals.org)
  • DEC-205, a 205-kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization. (doximity.com)
  • Purification and characterization of a light-harvesting chlorophyll-protein complex from the marine eustigmatophyte nannochloropsis sp. (bgu.ac.il)
  • Dive into the research topics of 'Purification and characterization of a light-harvesting chlorophyll-protein complex from the marine eustigmatophyte nannochloropsis sp. (bgu.ac.il)
  • Further characterization was done with amino acid sequencing, evaluation of the IgE-binding properties of raw and roasted hazelnut with enzyme allergosorbent test inhibition, assessment of cross-reactivity with different allergens by means of immunoblotting inhibition, and purification by means of HPLC. (nuthealth.org)
  • Protein Purification Reagents - 6xHis-TEV Protease -Tobacco Etch Virus (TEV) protease is a cysteine protease that is commonly used to remove fusion tags, including His, GST and MBP, at the N or C termini of recombinant proteins. (fishersci.com)
  • Recombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human IL1B (NP_000567.1). (genetex.com)
  • Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled and reliable process. (cellsignal.com)
  • In this study, the cloning and expression of mouse IL-33 gene in Escherichia coli (E. coli ), purification of recombinant protein, and generation of polyclonal antibody against IL-33 are described. (hindawi.com)
  • After induction and purification, the relative molecular mass (Mr) of the recombinant BabA protein was about 39 000. (bvsalud.org)
  • Their amino acid sequences suggest that they are mainly cytosolic or nuclear proteins partly associating with membranes (Talbot et al. (springer.com)
  • 2009 ). The designated dysbindin paralogs show very limited sequence homology which raised the question whether DBNDD1 and DBNDD2 are dysbindin-like proteins or proteins that share a less conserved domain with DTNBP1 in the context of otherwise unrelated sequences (Ghiani and Dell'Angelica 2011 ). (springer.com)
  • As an outcome human DBNDD1 revealed a high sequence identity to dysbindin domain-containing proteins from other Hominidae (e.g. (springer.com)
  • Proteins with high sequence identity to human DBNDD1 can also be found in evolutionarily more distant species (e.g. (springer.com)
  • Further analysis indicated that these two proteins possessed almost identical gene sequences, apart from two insertion/deletion events in predicted external loops present within the putative barrel-like structure of the proteins. (eur.nl)
  • The IL-33 cDNA sequences encode 270 and 266 amino acid polypeptides for human and mouse, respectively, corresponding to full-length proteins with calculated masses of 30 and 29.9 kDa [ 2 ]. (hindawi.com)
  • N-linked glycans are commonly found on proteins, but always on a specific amino acid within a specific sequence. (nist.gov)
  • Since it catalyzes the second key step in the shikimate pathway, which is crucial for the aromatic amino acid metabolism in bacteria, fungi, and plants, but not in mammals, DHQS is a potential target for new antimicrobial agents, anti-parasitic agents and herbicides. (nih.gov)
  • MurZ is also homologous to the aromatic amino acid biosynthetic enzyme enolpyruvyl shikimate phosphate synthase, the other enzyme known to catalyze an enolpyruvyl transfer. (tamu.edu)
  • Intracellular metabolism of 5,10-dideazatetrahydrofolic acid in human leukemia cell lines. (aspetjournals.org)
  • Late onset of symptoms in an atypical patient with the cblJ inborn error of vitamin B12 metabolism: diagnosis and novel mutation revealed by exome sequencing. (medlineplus.gov)
  • Based on the amino acid sequence homology and biological activity in macrophages, Shimamoto et al ( 15 ) identified that HLP was identical to MSP ( 15 ). (spandidos-publications.com)
  • The PfTRAMP homologue in P. vivax , here denoted as PvTRAMP, is a 340-amino-acid long antigen encoded by a single exon that could have a potential role in cytoadherence, as indicated by the presence of a thrombospondin structural homology repeat (TSR) domain. (biomedcentral.com)
  • The amino-terminal amino acid sequence of the protein (first 13 residues) matched that of the reported cDNA exactly. (aspetjournals.org)
  • Treatment with 4-vinylpyridine in the presence of 6M guanidine converted six of them into s-pyridylethyl cysteine residues are determines by amino acid analysis. (savedelicious.com)
  • The N-terminal 25 amino acids of the enzyme reveal a high degree of similarity to enolases from other sources. (uni-regensburg.de)
  • We performed a Basic Local Alignment Search Tool (BLAST) analysis to identify regions of local similarity between the human DBNDD1 and protein sequences from other species (Fig. 1 ). (springer.com)
  • Genes adjacent to pvtramp were identified in silico to determine the degree of similarity between the protein sequences encoded by equivalent chromosomic fragments in P. falciparum and Plasmodium knowlesi . (biomedcentral.com)
  • The RNA-dependent-RNA-polymerase (RdRp, or ns5) polypeptide among all four types is conserved with 67% amino acid sequence identity. (umsystem.edu)
  • Identified by screening an E. coli genomic library for clones that conferred phosphomycin resistance, murZ encoded a 419-amino-acid polypeptide and was mapped to 69.3 min on the E. coli chromosome. (tamu.edu)
  • The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. (bvsalud.org)
  • Sequencing of a 2.5-kb chromosomal Sma I- Pst I fragment containing the structural gene revealed an open reading frame encoding 537 amino acids, homologous to known pckA genes. (asm.org)
  • A total of 1,194 potential lactic acid bacterium (LAB) isolates were tentatively grouped and identified by repetitive element sequence-based PCR, followed by sequence-based identification using 16S rRNA and pheS genes from a selection of genotypically unique LAB isolates. (who.int)
  • Sequence analysis indicated that these isolates were identical and unique from other previously sequenced strains (6). (cdc.gov)
  • Sequencing was conducted on 23 influenza (H1N1)pdm09 isolates that were recovered from positive samples. (who.int)
  • There were two different mismatches in the probe target sites of the HA gene sequences of all isolates ( n = 23) with additional mismatches only at position 7 (template binding site) identified for all eight negative real-time RT-PCR isolates. (who.int)
  • Which of the P4Hs act on signaling peptides, and whether or not they differ in preference for proline in a certain sequence context is still unclear. (elifesciences.org)
  • One of the clones possesses an identical nucleotide sequence to the one corresponding to B cell CR2, whereas the other represents a differentially spliced transcript which lacks the exon 8b of B cell CR2. (nih.gov)
  • A R. palustris No. 7 PEPCK-deficient strain showed growth characteristics identical to those of the wild-type strain either anaerobically in the light or aerobically in the dark when a C 4 -dicarboxylic acid, such as succinate or malate, was used as a carbon source. (asm.org)
  • Human and mouse IL-33 are 55% identical at the amino acid level. (hindawi.com)
  • Separation and partial amino acid sequence analysis of each subunit indicate that the 40-kDa enzyme is formed by proteolytic processing of the 48k Da form. (lu.se)
  • In Escherichia coli , besides the PEPCK reaction, there is another pathway to form PEP from C 4 -dicarboxylic acid which involves the NAD- and NADP-dependent malic enzymes (MAEA and MAEB) and phosphoenolpyruvate synthase (PPS). (asm.org)
  • Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase. (tamu.edu)
  • The Escherichia coli gene murZ, encoding the enzyme UDP-N-acetylglucosamine enolpyruvyl transferase, has been cloned and sequenced. (tamu.edu)
  • 2019 ) was used, https://www.ebi.ac.uk/Tools/msa/clustalo/ ] of human DBNDD1 and similar protein sequences found by a BLAST search in other selected species. (springer.com)
  • The NKX2-5 gene on chromosome 5q34 consists of two exons which encode a 324 amino acid protein. (cdc.gov)
  • The gene encoding PvTRAMP was identified through an extensive search of the databases hosting the genome sequence of P. vivax . (biomedcentral.com)
  • One sample each from the implicated lot of Alphanate (TM), acute-phase serum from patient 2, and stool from patient 1 were positive for HAV RNA after amplification by polymerase chain reaction of the amino terminal region of that portion of the HAV genome coding for VP1. (cdc.gov)
  • Irrespective of the LAB biodiversity, a large majority of the sugar and amino acid metabolites were detected in all sourdough samples. (who.int)
  • This innovative processing method was applied to evaluate simulated exposure samples of remifentanil and carfentanil in water and their metabolites remifentanil acid and norcarfentanil in urine. (cdc.gov)
  • Amino-acid sequence alignment and inhibition studies showed that the barley aspartic proteinase resembles mammalian lysosomal cathepsin D (EC 3.4.23.5). (lu.se)
  • Strains deficient in all three enzymes cannot grow on C 4 -dicarboxylic acids as a sole carbon source, whereas strains retaining one of these enzymes are still able to grow ( 14 ). (asm.org)
  • The heterodimeric glycoprotein hormones have only been identified in vertebrates and are highly conserved in organisms from primitive rayfin fish (Chondrostei) to human in both primary sequences and functional characteristics. (biovendor.com)
  • 2021 ). The human DBNDD1 isoforms 1 (UniProtKB: Q9H9R9-1) and 2 (UniProtKB: Q9H9R9-2) differ only in the N-terminal region where 20 amino acids are additional in isoform 2. (springer.com)
  • Differs from human insulin by a single amino acid substitution. (drugfuture.com)
  • 95% of human hearts (66 of 68 hearts had a sequence variant) with septal defects and were detected by direct sequencing. (cdc.gov)
  • Purification and interaction analyses of two human lysosomal vitamin B12 transporters: LMBD1 and ABCD4. (medlineplus.gov)
  • Sequence alignment [Clustal Omega (Madeira et al. (springer.com)
  • Set up and perform experimental work including purification, concentration and separation of samples. (lu.se)
  • The sequence conservation of the putative dysbindin domain across all selected species is notable (Fig. 1 shaded region). (springer.com)
  • In Rhizobium species, a PEPCK-deficient mutant fails to grow on minimal medium with succinate or other citric acid cycle intermediates but can grow on glucose and glycerol as a sole carbon source, suggesting that PEPCK functions here as a key gluconeogenic enzyme ( 30 ). (asm.org)
  • It encodes the inactive pre-pro-HGF, a single chain of 728 amino acids (83 kDa), which includes a signal sequence ( 1 - 31 ), a heavy α chain (69 kDa), and a light β chain (34 kDa). (spandidos-publications.com)
  • His group ran the large-scale chemical and enzyme-based synthesis and purification of the material before providing it in a powdered form. (nist.gov)
  • The miraculin rewires the sweet receptors to temporarily identify acids as sugars. (howstuffworks.com)
  • Primer extension analysis identified a transcriptional start site 72 bp upstream of the pckA initiation codon and an upstream sequence similar to ς 70 promoters. (asm.org)
  • Sequence analysis of the predicted product revealed 44% identity to OrfR from Bacillus subtilis (K. Trach, J.W. Chapman, P. Piggot, D. LeCoq, and J.A. Hoch, J. Bacteriol. (tamu.edu)
  • Purification, crystallization and preliminary X-ray crystallographic analysis of MIL, a glycosylated jacalin-related lectin from mulberry (Morus indica) latex. (helmholtz-hzi.de)
  • Purification, crystallization and preliminary X-ray structure analysis of the banana lectin from Musa paradisiaca. (helmholtz-hzi.de)
  • In addition, DGGE band sequence analysis demonstrated the presence of Acetobacter sp. (who.int)
  • Field virus was distinguished from seven distinct genotypes on the basis of nucleotide and amino acid identity. (cdc.gov)
  • Therefore, amino acids in multifunctional docking sites of Met have been exchanged with specific binding motifs for downstream adaptor molecules in order to investigate the signaling potential of the HGF‑Met signaling pathway. (spandidos-publications.com)
  • This reaction is the first step in the gluconeogenic pathway, in which citric acid cycle intermediates are converted to hexose. (asm.org)
  • Conversely, PEPCK also functions in the anaplerotic pathway of some organisms, such as Anaerobiospirillum succiniciproducens ( 37 ), Alcaligenes eutrophus ( 39 ), Ruminococcus flavefaciens ( 40 ), Dipetalonema viteae ( 4 ), and Trypanosoma cruzi ( 6 ), where it forms OAA from PEP, which in turn enters the citric acid cycle. (asm.org)
  • 42 pathologic cardiac tissue samples were sequenced. (cdc.gov)
  • The haemagglutinin (HA) segments of A(H1N1)pdm09 from both real-time RT-PCR positive and negative samples were isolated and sequenced. (who.int)
  • The investigators suggest that somatic sequence variants occur with high frequency and are aetiologic in cardiac malformations. (cdc.gov)
  • These two groups are both involved in the production of short-chain fatty acids and lactate, which alter the pH of the environment, modulate the microbiota, and have other systemic properties [ 19 ]. (biomedcentral.com)
  • In contrast, isoform 3 (UniProtKB: Q9H9R9-3) carries a 100 amino acids long N-terminal sequence extension. (springer.com)
  • TEV protease specifically recognizes a seven amino acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser (ENLYFQG/S) and cleaves between Gln and Gly/Ser under a wide range of conditions. (fishersci.com)