• Aromatic amino acids have been shown to promote self-assembly of amyloid peptides, although the basis for this amyloid-inducing behavior is not understood. (nau.edu)
  • We adopted the amyloid-β 16-22 peptide (Aβ(16-22), Ac-KLVFFAE-NH 2 ) as a model to study the role of aromatic amino acids in peptide self-assembly. (nau.edu)
  • Previous studies in the literature have resulted in conflicting reports on the potential neurotoxicity of the beta-cleaved Alzheimer's disease C-terminal fragment (beta-CTF) of beta-amyloid precursor protein in vivo. (amsterdamumc.org)
  • All recognized mutations for AD are associated with increased deposition of amyloid beta (Abeta), a peptide fragment comprising 39-43 amino acids that derives from the catabolism of the amyloid precursor protein (APP) molecule. (medscape.com)
  • A multi-coronavirus protein microarray used to map the binding of COVID-19 patient antibodies to SARS-CoV-2 proteins and protein fragments as well as to the proteins of four other coronaviruses that infect humans has shown specific regions of SARS-CoV-2 proteins that are highly reactive with patient antibodies and revealed cross-reactivity of these antibodies with other human coronaviruses. (cdc.gov)
  • The proteasome is a molecular machine that fragments proteins into short peptides. (yale.edu)
  • The standard experiment is to separate the proteins, e.g. by 2D gel electrophoresis, digest the proteins with a highly specific enzyme, measure the masses of the peptide fragments with a mass spectrometer (typically a MALDI-TOF mass spectrometer) and then compare the peptide monoisotopic masses with expected monoisotopic masses from a database (protein or DNA database). (lu.se)
  • Peptide analysis identified products from known matrix protease substrates and secretory proteins, which offered functional relevance to immunoinflammatory responses, extracellular matrix remodeling, and receptor interactions. (cdc.gov)
  • A multi-coronavirus protein microarray was created containing full-length proteins, overlapping protein fragments of various lengths, and peptide libraries from SARS-CoV-2 and four other human coronaviruses. (cdc.gov)
  • Antibody reactivity against overlapping 100-, 50-, and 30-amino acid fragments of SARS-CoV-2 proteins was used to identify antigenic regions. (cdc.gov)
  • N-terminal B-type natriuretic peptide or troponin elevations, or the Background use of inotropes during admission, are much more powerful and Risk stratification and prediction is an integral part of clinical accurate predictors than admission to hospital alone. (who.int)
  • Myocardial angiotensin metabolites and enzymatic activities involved in the metabolism of the key angiotensin peptides angiotensin 1-8 (AngII) and Ang1-7 were determined in left ventricular samples by mass spectrometry. (kl.ac.at)
  • Scholars@Duke publication: Circulating biomarkers for abdominal aortic aneurysm: what did we learn in the last decade? (duke.edu)
  • Serum elastase peptides seem still to be sufficient biomarkers to predict expansion and rupture, but advanced techniques (ELISA) and larger studies are needed to establish its exact role. (duke.edu)
  • This approach fails to recognize novel peptides since it can only match to existing sequences in the database. (wikipedia.org)
  • Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. (abcam.com)
  • The amino acid sequence of the peptide epitope was compared to six other known virus peptides known to be restricted by HLA B8 and a sequence homology was identified, which predicted nonamer and octamer epitope sequences. (ox.ac.uk)
  • The Role of Hybrid Insulin Peptides in the Development of Type 1 Diabetes. (diabetes.org)
  • We found that diabetes-inducing T cells, isolated from diabetic mice, recognize antigens formed by cross-linking of insulin fragments to other protein fragments (peptides). (diabetes.org)
  • The resulting hybrid insulin peptides (HIPs) are antigenic for T cells and can be detected by mass spectrometry in beta cell extracts. (diabetes.org)
  • He has been the Head of the Insulin Peptides Laboratory since 2012 at the now- Florey Institute of Neuroscience and Mental Health, University of Melbourne. (eurekaselect.com)
  • His research group is interested in designing peptidomimetics and developing novel chemical methods for preparing therapeutic insulin and insulin-like peptides. (eurekaselect.com)
  • This is possible in the presence of another binding site for the S1 protein beside the receptor-binding domain for ACE2, which binds the peptide QAKTFLDKFNHEAEDLFYQ. (scilifelab.se)
  • Aptamers as Novel Binding Molecules on an Antimicrobial Peptide-Armored Composite Hydrogel Wound Dressing for Specific Removal and Efficient Eradication of Pseudomonas aeruginosa. (mpg.de)
  • The C-terminal 32-mer fragment of hemoglobin alpha is an amyloidogenic peptide with antimicrobial properties. (mpg.de)
  • Granulysin is an antimicrobial peptide (AMP) expressed by human T-lymphocytes and natural killer cells. (uni-ulm.de)
  • BACHD mice with ubiquitous expression of full-length mutant HTT (mHTT) and wild-type mice with targeted hypothalamic overexpression of either wild-type HTT (wtHTT) or mHTT fragments. (lu.se)
  • Scholars@Duke publication: A serum proteomic approach to gauging the state of remission in Wegener's granulomatosis. (duke.edu)
  • To investigate if this specific cleavage has pathological relevance in fibrotic disorders, we developed a competitive ELISA targeting the generated neo-epitope on the released fragment and quantified it in serum from patients with lung cancer, idiopathic pulmonary fibrosis (IPF), chronic obstructive pulmonary disease (COPD) and healthy subjects. (dtu.dk)
  • The dominant epitope was mapped using recombinant vaccinia viruses that expressed fragments of the NP and then synthetic peptides based on the NP amino acid sequence. (ox.ac.uk)
  • One of the predicted epitope peptides was made and shown to be recognized by specific CTL. (ox.ac.uk)
  • For over two decades, Artur Schmidtchen and his research group have studied endogenous peptides, smaller protein fragments in the body with the ability to both kill bacteria and neutralize the bacterial toxins released during an infection. (lu.se)
  • This binding is mediated by the alpha(2LG1, alpha2LG4, and alpha2LG5 modules present in the naturally cleaved fragments of the peripheral nerve laminin alpha2 chain, and is inhibited by the synthetic terminal trisaccharide of PGL-1. (ed.ac.uk)
  • Synthetic peptide. (abcam.com)
  • For this b-y ion pair, the sum of their subscripts is equal to the total number of amino acid residues in the unknown peptide. (wikipedia.org)
  • Probable anchor residues were identified at peptide residues 3 (lysine/arginine), 5 (lysine/arginine) and 9 (leucine/isoleucine). (ox.ac.uk)
  • A model was made of the HLA B8 molecule and negatively charged pockets predicted, which could accommodate the positively charged side chains of the peptide anchor residues. (ox.ac.uk)
  • We study the effect of the peptide QAKTFLDKFNHEAEDLFYQ on the kinetics of the SARS-CoV-2 spike protein S1 binding to angiotensin-converting enzyme 2 (ACE2), with the aim to characterize the interaction mechanism of the SARS-CoV2 virus with its host cell. (scilifelab.se)
  • De novo sequencing is an assignment of fragment ions from a mass spectrum. (wikipedia.org)
  • The N-terminal charged fragment ions are classed as a, b or c, while the C-terminal charged ones are classed as x, y or z. (wikipedia.org)
  • Among these sequence ions, a, b and y-ions are the most common ion types, especially in the low-energy collision-induced dissociation (CID) mass spectrometers, since the peptide amide bond (CO-NH) is the most vulnerable and the loss of CO from b-ions. (wikipedia.org)
  • Most fragment ions are b- or y-ions. (wikipedia.org)
  • Neutral loss of ammonia from Arg leads to fragment ions (y-17) or (b-17) ions with higher abundance than their corresponding ions. (wikipedia.org)
  • In high energy CID, all different types of fragment ions can be observed but no losses of ammonia or water. (wikipedia.org)
  • Now, drawing inspiration from the body's own defence mechanisms based on peptides, we have developed a new approach to treating severe bacterial infections", says Artur Schmidtchen, professor in dermatology and venereology and senior consultant at Skåne University Hospital, who led the study. (lu.se)
  • Knowing the amino acid sequence of peptides from a protein digest is essential for studying the biological function of the protein. (wikipedia.org)
  • Database search is a simple version as the mass spectra data of the unknown peptide is submitted and run to find a match with a known peptide sequence, the peptide with the highest matching score will be selected. (wikipedia.org)
  • Factors affecting fragmentation are the charge state (the higher charge state, the less energy is needed for fragmentation), mass of the peptide (the larger mass, the more energy is required), induced energy (higher energy leads to more fragmentation), primary amino acid sequence, mode of dissociation and collision gas. (wikipedia.org)
  • This peptide corresponds to the sequence 24-42 of the ACE2 α1 domain, which marks the binding site for the S1 protein. (scilifelab.se)
  • A sequence pattern for peptides presented to cytotoxic T lymphocytes by HLA B8 revealed by analysis of epitopes and eluted peptides. (ox.ac.uk)
  • The effective substrates displayed heparin-binding peptides, which can interact with cell-surface glycosaminoglycans and could be used with a defined medium to culture hES cells for more than 3 months. (nih.gov)
  • Peptide Bispecifics Inhibiting HIV-1 Infection by an Orthogonal Chemical and Supramolecular Strategy. (mpg.de)
  • These peptides have also proven to be powerful tools in managing the harmful inflammatory response that occurs in the body as a result of the infection. (lu.se)
  • The peptides´ functions are crucial - if the infection and inflammation are not contained, it can lead to sepsis. (lu.se)
  • Support for this pattern came from sequencing peptides eluted from purified HLA B8 molecules, where lysines were predominant at positions 3 and 5. (ox.ac.uk)
  • Custom Workflow for the Confident Identification of Sulfotyrosine-Containing Peptides and Their Discrimination from Phosphopeptides. (liverpool.ac.uk)
  • The strategy therefore promises reliable automated user- independent protein identification using peptide mass fingerprints. (lu.se)
  • Cleavage of SPARC at a single peptide bond, increases the affinity for collagens up to 20-fold. (dtu.dk)
  • Data-mining unveils structure-property-activity correlation of viral infectivity enhancing self-assembling peptides. (mpg.de)
  • Inverse design of viral infectivity-enhancing peptide fibrils from continuous protein-vector embeddings. (mpg.de)
  • It includes information on projects, publications, research funders and collaborations. (chalmers.se)
  • He is the Editor-in-Chief of two Bentham journals, Protein and Peptide Letters, for research reports and mini-reviews, and Current Protein and Peptide Science, which publishes exclusively review papers. (eurekaselect.com)
  • His research interests are in the field of peptide drug discovery. (eurekaselect.com)
  • Dr. Weber has published more than a number of scientific publications and has served on the editorial boards of a number of journals and was also appointed as Associate Editor of this journal (Protein & Peptide Letters), published by Bentham Science Publishers. (eurekaselect.com)
  • Three different types of backbone bonds can be broken to form peptide fragments: alkyl carbonyl (CHR-CO), peptide amide bond (CO-NH), and amino alkyl bond (NH-CHR). (wikipedia.org)
  • Ala and Tyr position 19 variants failed to undergo fibril formation at the peptide concentrations studied, but Cha and F 5 -Phe variants self-assembled at dramatically enhanced rates relative to wild-type. (nau.edu)
  • Today, analysis by a tandem mass spectrometer is a more common method to solve the sequencing of peptides. (wikipedia.org)
  • Thyroglobulin was digested by trypsin in distilled water and the resulting peptides were identified by TOF-secondary ion mass spectrometry, using TFA as a matrix to catalyze the ionization of the peptides. (chalmers.se)
  • The sections were analyzed with TOF-secondary ion mass spectrometry, and the peptides formed were identified through comparison with the peptides of the thyroglobulin reference sample. (chalmers.se)
  • Furthermore, we report a side reaction correlated with the coupling steps and generating truncated fragments with a mass deviation of + 42 Da. (pasteur.fr)
  • A narrative review was undertaken by searching for original and review articles no older than five years since publication enfermedad celíaca y through databases consulted: HINARI, PubMed and Scopus in Spanish and English. (bvsalud.org)
  • Se realizó una búsqueda en bases de datos HINARI, PubMed y Scopus en idioma español e inglés, se incluyeron artículos originales y de revisión con un máximo de cinco años desde su publicación. (bvsalud.org)
  • The kinetic analysis of S1-ACE2 complex dissociation revealed that preincubation stabilized this complex, and this effect was dependent on the peptide concentration as well as the preincubation time. (scilifelab.se)
  • C-terminally truncated fragments of human α-calcitonin gene-related peptide (h-α-CGRP) were tested for their ability to stimulate amylase secretion from pancreatic acinar cells and relax precontracted mesenteric arteries. (uaeu.ac.ae)