• Although low-affinity ligands induced phosphorylation of the Cd3 chains of the T cell receptor and the interaction of Cd3 with the Zap70 kinase as strongly as high-affinity ligands, they failed to activate Zap70 to the same extent. (nature.com)
  • G protein-coupled receptor kinase 5 [So. (gsea-msigdb.org)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) in samples from tissue homogenates or other biological fluids. (worldcarecouncil.org)
  • The phosphorylation of SIRPα ITIMs induces the recruitment and activation of the tyrosine phosphatases PTPN6 and PTPN11, resulting in the negative regulation of several biological processes. (biolegend.com)
  • These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. (cusabio.com)
  • While the phosphorylation of tyrosine residues in the immunoreceptor tyrosine-based activation motifs (ITAMs) of Cd3 and the inducible interaction between Cd3 and Zap70 remained largely unaffected, the abundance of activated Zap70 dropped with decreasing peptide affinity. (nature.com)
  • Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. (cusabio.com)
  • Protein phosphatases play important roles in cell signaling processes in tandem with kinases. (reactionbiology.com)
  • Different than kinases, phosphatases were long considered undruggable because their active site is highly conserved resulting in selectivity issues and an unfavorable molecular composition leading to bioavailability issues. (reactionbiology.com)
  • The kinases have been implicated in controlling the amplitude of a signalling response, whereas phosphatases are thought to have an important role in controlling the rate and duration of the response [ 1 , 2 ]. (umh.es)
  • Alteration in the gene encoding protein tyrosine phosphatase nonreceptor type 6 (PTPN6/SHP1) may contribute to neutrophilic dermatoses. (rush.edu)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human G Protein Coupled Receptor 78 (GPR78) in Tissue homogenates and other biological fluids. (1elisakits.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Activin A Receptor Type II A (ACVR2A) in tissue homogenates, cell lysates and other biological fluids. (lotuskringpoeldijk.nl)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Activin A Receptor Type II A (ACVR2A) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (lotuskringpoeldijk.nl)
  • The efficacy of a therapeutic antibody depends on the Fab fragment and its binding activity to the target antigen, but also depends on the Fc fragment and its interaction with key Fc receptors.Therefore, candidates must be tested against a panel of receptors during antibody engineering. (acrobiosystems.com)
  • Description: A sandwich ELISA kit for detection of Protein Tyrosine Phosphatase, Non Receptor Type 11 from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (worldcarecouncil.org)
  • Description: A sandwich ELISA kit for detection of G Protein Coupled Receptor 78 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (1elisakits.com)
  • Here, we used time-resolved high-throughput proteomic analyses to identify and quantify the phosphorylation events and protein-protein interactions encoding T cell ligand discrimination in antigen-experienced T cells. (nature.com)
  • This is consistent with reports that the slow phosphorylation of tyrosine residues of the Lat adaptor associates with recruitment and activation of the phospholipase Plcγ1, thereby constituting an important kinetic bottleneck for ligand discrimination 11 . (nature.com)
  • We defined quantitative signatures of ligand affinity based on protein phosphorylation and protein-protein interaction (PPI) stoichiometry for critical molecular events associated with TCR signaling. (nature.com)
  • The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. (cancerindex.org)
  • The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. (cancerindex.org)
  • Spatial Transcriptomics Depict Ligand-Receptor Cross-talk Heterogeneity at the Tumor-Stroma Interface in Long-Term Ovarian Cancer Survivors. (viictr.org)
  • This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling, (e.g., the LYN-CD22-SHP-1 pathway). (wikipedia.org)
  • As shown recently, polySia-modified proteins can also be released by activated microglia, and the intrinsically released protein-bound and exogenously applied free polySia were equally able to attenuate proinflammatory microglia activation via the inhibitory immune receptor Siglec-E. Here, we explore polySia as a candidate substance for promoting myelin regeneration by immunomodulation. (for2953-sia.de)
  • According to the kinetic proofreading model, antigen discrimination properties could be explained by the gradual amplification of small differences in binding affinities as the signal is transduced downstream of the T cell receptor. (nature.com)
  • In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. (cusabio.com)
  • however, as no receptor-like isoforms of HePTP (PTPN7) have been identified, this is included with the non-transmembrane enzymes. (umh.es)
  • As a result, formation of the signalosome of the Lat adaptor was severely impaired with low- compared with high-affinity ligands, whereas formation of the signalosome of the Cd6 receptor was affected only partially. (nature.com)
  • The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. (cancerindex.org)
  • N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. (wikipedia.org)
  • In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. (cancerindex.org)
  • Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD47 4 , in vivo blocking of dendritic cell migration 3 , enhancing of macrophage phagocytosis 2,4 , immunohistochemical staining of cerebellum frozen sections 1 , immunoprecipitation 2,4 , and spatial biology (IBEX) 5,6 . (biolegend.com)
  • The classical protein tyrosine phosphatases (PTPs) can be categorized as receptor-like (R) or non-transmembrane (NT) proteins. (umh.es)
  • For those receptor-like PTPs (RPTP) with two intracellular PTP domains, the membrane-proximal D1 domain is catalytically active. (umh.es)
  • CD172a, also known as SIRPa, is a type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. (biolegend.com)
  • Following dimerization, TLR signals are transduced via a cytoplasmic C-terminal Toll IL-1 receptor (TIR) domain to a set of adapter proteins. (antibodies-online.com)
  • Our phosphatase assays are based on the dephosphorylation of the fluorinated MUP ( methylumbelliferyl phosphate) derivative which serves as a substrate for most protein phosphatases. (reactionbiology.com)
  • Dephosphorylation of MUP generates the fluorescent DiFMUP (6,8-difluoro-4-methylumbelliferyl phosphate) which is quantified for the determination of phosphatase activity. (reactionbiology.com)
  • After homogenization, the samples were submitted to differential centrifugation to obtain cell membranes and, further, to phospholipase C (PIPLC) treatment, to remove membrane-bound proteins anchored by phosphatidylinositol. (bvsalud.org)
  • The average of specific activity for pNPP hydrolysis (at pH 10.5) by alkaline phosphatase released by phosphatidylinositol-specific phospholipase C (PIPLC) from Bacillus cereus among different bone regions at different animal ages was 1,142.57 U.mg-1, while for PPi hydrolysis (at pH 8.0), it was 1,433.82 U.mg-1. (bvsalud.org)
  • Recruitment and activation of SHP-1 protein-tyrosine phosphatase by human platelet endothelial cell adhesion molecule-1 (PECAM-1). (wikipedia.org)
  • Both treatments increased B-cell bcl-2 and shp-1 gene expression and enhanced ICAM-1 and VCAM-1 protein levels in GC B cells. (nih.gov)
  • Tyrosine-protein phosphatase non-receptor type 6, also known as Src homology region 2 domain-containing phosphatase-1 (SHP-1), is an enzyme that in humans is encoded by the PTPN6 gene. (wikipedia.org)
  • The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. (wikipedia.org)
  • Different mutations of EDA gene and EDAR gene could make different influence on the protein function and lead to the occurrence of HED. (bvsalud.org)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • with Pubmed ID, gene symbol/accession number and viral interacting protein. (polygenicpathways.co.uk)
  • Chronic inflammation is connected to neoplastic transformation, and it is known that patients with autoimmune diseases have increased risks for some types of tumors [ 3 , 4 ], with neoplastic transformation often occurring [ 5 - 8 ]. (medsci.org)
  • A Potent and Selective Small-Molecule Inhibitor for the Lymphoid-Specific Tyrosine Phosphatase (LYP), a Target Associated with Autoimmune Diseases. (umh.es)
  • Both chlordecone and E2 activated splenic B cells and enhanced GC reactions, as shown by upregulated protein expression of GL7, CXCR5, and CXCR4. (nih.gov)
  • Toll-like receptors in the pathogenesis of human disease. (antibodies-online.com)
  • Pre-formed fibrils are an invaluable preclinical model for exploring pathogenesis of neurological diseases through aggregation of misfolded proteins. (acrobiosystems.com)
  • Both pathways culminate in the induction of inflammatory cytokines (TNF, IL-6, IL-12), type I interferons (IFN-alpha, IFN-beta), or apoptosis. (antibodies-online.com)
  • Ryanodine receptor 1-mediated Ca2+ signaling and mitochondrial reprogramming modulate uterine serous cancer malignant phenotypes. (viictr.org)
  • As part of the innate immune system the Toll-like receptor (TLR) signaling pathway contributes to the first line of defense against microbial pathogens. (antibodies-online.com)
  • Toll-Like Receptor 3 Signaling via TRIF Contributes to a Protective Innate Immune Response to Severe Acute Respiratory Syndrome Coronavirus Infection. (antibodies-online.com)
  • The study of pNPP and PPi hydrolysis by the enzyme revealed that the optimum pH of actuation for pNPP was 10.5, while for PPi, which is considered the true substrate of alkaline phosphatase, was 8.0, close to the physiological value. (bvsalud.org)
  • Description: A sandwich quantitative ELISA assay kit for detection of Mouse Neurotensin Receptor 1, High Affinity (NTSR1) in samples from tissue homogenates or other biological fluids. (1elisakits.com)
  • Streptavidin is a tetrameric protein providing 4 high-affinity biotin binding sites. (acrobiosystems.com)
  • The results show that regardless of the ossification type that occurs, the same enzyme or isoenzymes act on the different bone regions and different life stages of anurans. (bvsalud.org)
  • For the remaining RPTPs, including PTPepsilon, which is the other enzyme in receptor subtype R4, the D2 domain maintains a PTP fold but lacks activity and can be classified as a pseudophosphatase domain. (umh.es)
  • The discovery of an allosteric SHP2 inhibitor paved the road for the creation of further allosteric inhibitors of phosphatases. (reactionbiology.com)
  • Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. (cusabio.com)
  • e.g. lipopolysaccharide (LPS) of gram-negative bacteria are recognized by TLR4 while TLR5 recognizes the bacterial protein flagellin. (antibodies-online.com)
  • Reaction Biology offers phosphatase assays for compound screening and profiling for drug discovery. (reactionbiology.com)
  • At Reaction Biology, we offer phosphatase profiling and screening services for pharma, biotech, and academic institutions with a commitment to quality data and quality service. (reactionbiology.com)
  • Whether it is for protein electrophoresis or western blot, our pre-stained protein markers help you quickly determine the molecular weight of the target protein or evaluate the transfer efficiency. (acrobiosystems.com)
  • Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. (cancerindex.org)
  • We used affymetrix to characterize the effect of Mineralocorticoid Receptor deletion on macrophage transcriptional profile, and identify its requirement in normal glucocorticoid signalling. (gsea-msigdb.org)
  • Inappropriate excess of the steroid hormone aldosterone, which is a mineralocorticoid receptor (MR) agonist, is associated with increased inflammation and risk of cardiovascular disease. (gsea-msigdb.org)
  • Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. (viictr.org)
  • Protein markers are an essential component in biological research and drug development. (acrobiosystems.com)
  • T-cell development ensures the formation of diverse repertoires of T-cell receptors (TCRs) that recognize a variety of antigens. (for2953-sia.de)
  • C57BL/6 mouse bone marrow cells were stained with CD11b PE and CD172a (clone P84) APC/Fire™ 750 (top) or rat IgG1, κ APC/Fire™ 750 isotype control (bottom). (biolegend.com)
  • The farnesoid-X-receptor (FXR) protects against inflammation and cancer of the colon through maintenance of intestinal bile acid (BA) homeostasis. (cancerindex.org)