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  • Humans
  • In humans, a PMN gene signature correlated with improved survival in several cancer subtypes, including PTEN-deficient uterine cancer. (nih.gov)
  • phosphatase
  • PTEN protein, a phosphatase involved in the regulation of Akt phosphorylation, was present at all days of estrous cycle and showed no significant regulation in relation to cycle. (nih.gov)
  • In inner ear development, phosphatase and tensin homolog (PTEN) is necessary for neuronal maintenance, such as neuronal survival and accurate nerve innervations of hair cells. (nih.gov)
  • The mRNA levels of mammalian target of rapamycin (mTOR), SH2 domain‑containing inositol‑5'‑phosphatase 2 (SHIP2), phosphatase and tensin homologue (PTEN) and breakpoint cluster region (BCR)/Abelson (ABL) were detected pre and post‑virosecurinine treatment using reverse transcription quantitative polymerase chain reaction (RT‑qPCR). (nih.gov)
  • Using the conserved PTEN catalytic core motif, we have identified a new protein in the Dictyostelium genome called phospholipid-inositol phosphatase (PLIP), which defines a new subfamily of phosphoinositide phosphatases clearly distinct from PTEN or other closely related proteins. (nih.gov)
  • inhibited PTEN expression
  • Kaposi's sarcoma (KS) is an AIDS-defining cancer with aberrant neovascularization caused by KS-associated herpesvirus (KSHV).Furthermore, Nef and K1 induced cellular miR-718, which inhibited PTEN expression by directly targeting a seed sequence in the 3' UTR of its mRNA.Our results demonstrate an essential role of miR-718/AKT/mTOR axis in AIDS-KS and thus may represent an attractive therapeutic target. (nih.gov)
  • Mice
  • Ablation of Raptor also significantly extends survival of mice in models of leukemogenesis evoked by Pten deficiency. (biomedsearch.com)
  • We previously reported that Pten conditional knockout (cKO) mice exhibited disorganized fasciculus with neuronal apoptosis in spiral ganglion neurons (SGNs). (nih.gov)
  • To better understand the genes and signaling networks related to auditory neuron maintenance, we compared the profiles of differentially expressed genes (DEGs) using microarray analysis of the inner ear in E14.5 Pten cKO and wild-type mice. (nih.gov)
  • This result was consistent with the phenotypic defects of SGNs in Pten cKO mice (e.g., neuronal apoptosis, abnormal migration, and irregular nerve fiber patterns of SGNs). (nih.gov)
  • Consistent with the microarray results, expression levels of Spp1 and Rgs4 were increased in the Pten cKO compared to wild-type mice. (nih.gov)
  • We found that the levels of Pvalb, a neuronal marker , were downregulated (Fig. S1E, F). Reduced levels of Pvalb expression may be explained by the loss of Pvalb-expressing neurons in Pten-deficient mice. (nih.gov)
  • PTEN is a tumor suppressor in CML stem cells and BCR-ABL induced leukemias in mice. (umassmed.edu)
  • In addition, overexpression of PTEN delays the development of CML and B-ALL, and prolongs survival of leukemia mice. (umassmed.edu)
  • We demonstrate here that loss of Pten is similar to AKT activation in the context of glioma formation in mice. (nih.gov)
  • B) PCR analysis indicates that RCAS-Cre resulted in Pten deletion in some of the neural progenitors (lane 1, upper band) from Ntv-a LP mice, but not in other types of primary brain cells or uninfected neural progenitors (lane 1, lower band). (nih.gov)
  • C) Western blot analysis shows that Cre decreased Pten protein level and consequently activated the Akt pathway, but not the MAPK pathway, in neuronal progenitors from Ntv-a LP mice. (nih.gov)
  • mTOR
  • mTOR complex 1 plays critical roles in hematopoiesis and Pten-loss-evoked leukemogenesis. (biomedsearch.com)
  • HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway. (nih.gov)
  • The tumors were further used to determine whether PTEN/AKT/mTOR pathway was involved in the synergistic effect of Nef and K1 on tumorigenesis in vivo. (nih.gov)
  • As shown in Figure 5A, the phosphorylated forms of PI3K, AKT and mTOR were increased in tumors induced by K1 or Nef, which were even more striking in tumors induced by both K1 and Nef, whereas the levels of total PTEN were reduced accordingly. (nih.gov)
  • Dysregulation of the mammalian target of rapamycin (mTOR) signaling has been found in many human cancers, particularly those with loss of the tumor suppressor PTEN. (nih.gov)
  • RT‑qPCR indicated that virosecurinine upregulated the gene expression of PTEN and downregulated the expression of mTOR, SHIP‑2 and BCR/ABL in K562 cells. (nih.gov)
  • Virosecurinine inhibited the growth and proliferation of the K562 cell lines and induced apoptosis in K562 cells by affecting the expression of mTOR, SHIP2, BCR/ABL and PTEN. (nih.gov)
  • In order to further understand the molecular mechanism underlying virosecurinine-induced apoptosis, the mRNA levels of mTOR, PTEN, BCR/ABL and SHIP2 mRNA were measured in virosecurinine-deprived and control K562 cells using RT-qPCR analysis. (nih.gov)
  • mTOR promotes survival and astrocytic characteristics induced by Pten/AKT signaling in glioblastoma. (nih.gov)
  • Mechanistic justification for the therapeutic blockade of targets downstream of AKT, such as mTOR, in these cancers requires demonstration that the oncogenic effect of PTEN loss is through elevated AKT activity. (nih.gov)
  • Mutation
  • Therefore, we investigated PI3K/AKT genetic alterations such as PIK3CA amplification, PIK3CA mutation, PTEN protein loss and their relationships with various clinicopathological characteristics in 156 EOCs. (nih.gov)
  • Differences in mutation profiles and patterns of gene gains/losses are seen between BRCA2 (associated with TP53/PTEN mutations, loss of RB1 and gain of HRAS, STK11 and SMARCB1) and BRCAX (associated with PIK3CA mutations) tumours, suggesting that BRCA2 and BRCAX MBCs may be distinct and arise from different tumour pathways. (edu.au)
  • deletion
  • That patient was observed to have a deletion in the critical portion of Exon 5 of the PTEN gene, the gene associated with Cowden disease. (biomedsearch.com)
  • Using our mouse model of BCR-ABL induced leukemias, we show that PTEN is down-regulated by BCR-ABL in leukemia stem cells (LSCs) in CML, and that PTEN deletion causes acceleration of CML development. (umassmed.edu)
  • Combined RCAS/tv-a and Cre/Lox systems result in Pten deletion and consequent Akt, but not MAPK pathway activation. (nih.gov)
  • In HCT116 cells, activation mutations in the PI3K catalytic subunit PIK3CA and Ki-Ras correlated with a resistant phenotype, which was partially sensitized by homologous replacement with the wild-type Ki-Ras but not by deletion of cellular PTEN. (nih.gov)
  • mutations
  • Because inactivation of PTEN is a somatic event, PTEN mutations represent an important genetic difference between cancer cells and normal cells and therefore a potential anticancer drug target. (biomedsearch.com)
  • In an effort to identify small molecules that preferentially kill cells with mutations of PTEN, the authors developed and implemented a high-throughput, paired cell-based screen composed of parental HCT116 cells and their PTEN gene-targeted derivatives. (biomedsearch.com)
  • Overall, PIK3CA mutations are most commonly seen, with fewer TP53 and PTEN mutations, similar to the profile seen in luminal A female breast cancers. (edu.au)
  • Because β-catenin activation and Myc translocations were previously found in murine T-cell acute lymphoblastic leukemias (T-ALLs) deficient for Pten, our results suggest that activation of the canonical Wnt pathway is associated with a subtype of Notch-independent T-ALLs that bear Myc gene rearrangements and Pten mutations. (ox.ac.uk)
  • inhibition
  • Pharmacological inhibition of PTEN limits myocardial infarct size and improves left ventricular function postinfarction. (biomedsearch.com)
  • therefore, the inhibition of PTEN enhances PI3K/Akt signaling and could prevent myocardium from ischemia-reperfusion (I/R) injury. (biomedsearch.com)
  • Here we studied 1) whether the pharmacological inhibition of PTEN by bisperoxovanadium molecules [BpV(HOpic)] attenuates simulated I/R (SIR) injury in vitro and 2) whether the administration of BpV(HOpic) either before or after ischemia limits myocardial infarct size (IS) and ameliorates cardiodysfunction caused by infarction. (biomedsearch.com)
  • In conclusion, the pharmacological inhibition of PTEN protects against I/R injury through the upregulation of the PI3K/Akt/eNOS/ERK prosurvival pathway, suggesting a new therapeutic strategy to combat I/R injury. (biomedsearch.com)
  • The inhibition of PTEN, a negative regulator of AKT, or the expression of a constitutively active form of AKT restores myelin outgrowth in cultured beta1-deficient oligodendrocytes. (plymouth.ac.uk)
  • Inhibition of PTEN and activation of Akt may contribute to the anti-oxidant capacity and cardioprotection of ABPP. (nih.gov)
  • pathway
  • Interactions between PTEN and the c-Met pathway in glioblastoma and implications for therapy. (biomedsearch.com)
  • Using laser microdissection technology and real-time PCR, together with immunohistochemistry, we have explored the growth factor and integrin dependent PI3-kinase/PTEN/Akt signalling pathway in prostate cell lines and tumour samples by analysing EGF-R, IGF1-R, ILK, beta3 integrin, PTEN and p-Akt protein expression. (uni.lu)
  • Extending these methods to endogenous proteins, we have shown that well-level results of the knockdown of PTEN results in an increase in phospho-S6 levels, but at the single cell level, the correlation reveals the role of other inputs into the pathway. (nih.gov)
  • metastasis
  • Complete loss of PTEN expression as a possible early prognostic marker for prostate cancer metastasis. (uni.lu)
  • We therefore compared PTEN expression in patient biopsies at first time diagnosis recruited prospectively (Study I, 112 patients) and patients with confirmed metastasis recruited retrospectively from the Luxembourg cancer registry (Study II, 42 patients). (uni.lu)
  • In Study II, 25 of the 42 patients (59%) with lymph node metastasis had complete loss of PTEN expression in both the neoplastic glands of the prostate and the invasive prostate cancer cells in the lymph node, and of these 13 (52%) exhibited already loss of PTEN expression at first diagnosis. (uni.lu)
  • These findings demonstrate that loss of PTEN expression is an important factor in progression towards metastatic disease and could potentially serve as an early prognostic marker for prostate cancer metastasis. (uni.lu)
  • Proteins
  • TQ treatment increased cellular levels of PTEN proteins, resulting in a substantial decrease of phosphorylated Akt, a known regulator of cell survival. (nih.gov)
  • Fluorescence
  • To confirm the PCa origin of CTCs, IF was used for androgen receptor (AR) expression and fluorescence in situ hybridization was used for PTEN and ERG assessment. (nih.gov)
  • tumors
  • In human GBMs, AKT is not mutated but is activated in approximately 70% of these tumors, in association with loss of PTEN and/or activation of receptor tyrosine kinases. (nih.gov)
  • overexpression
  • β-Catenin activation synergizes with Pten loss and Myc overexpression in Notch-independent T-ALL. (ox.ac.uk)
  • They exhibit recurrent secondary genomic rearrangements that lead to Myc overexpression and loss of Pten activity. (ox.ac.uk)
  • neoplastic
  • We provide evidence that loss of PTEN expression rather than upregulated EGF/IGF1 receptor expression was responsible for increased p-Akt in neoplastic prostate cells. (uni.lu)
  • expression
  • We observed increased levels of Spp1 (also known as osteopontin, Opn) and Rgs4 expression in Pten-deficient SGNs compared to the wild-type (Fig. 2). (nih.gov)
  • Recombinant eukaryotic expression plasmid containing human wild-type PTEN gene was transfected into C13K cells by lipofectamine2000. (biomedsearch.com)
  • Transfection of PTEN could increase the expression of PTEN and restore drug sensitivity to cisplatin in human ovarian cancer cell line C13K with multidrug-resistance by decreasing the expression of p-Akt. (biomedsearch.com)
  • leukemias
  • These results demonstrate a critical role of PTEN in BCR-ABL induced leukemias and suggest a potential strategy for the treatment of Ph(+) leukemia. (umassmed.edu)
  • recurrent
  • These findings spurred the UCLA group to design an important, molecularly focused clinical study, published in this issue of PLoS Medicine, to analyze the effect of rapamycin in a subset of patients with recurrent GBM in whom activity of the tumor suppressor PTEN was absent. (nih.gov)
  • phosphatases
  • Signaling pathways dependent on phosphoinositides phosphorylated at the D3 position of this ring (3-phosphoinositides) are negatively regulated by 3-phosphoinositide-specific phosphatases that include PTEN and myotubularin. (nih.gov)
  • loss
  • Frequently, loss of PTEN function is a common feature in both types of GBMs. (nih.gov)
  • describe
  • Using a mouse model of PTEN-deficient uterine cancer, we describe a surprising inhibitory role for PMNs in epithelial carcinogenesis. (nih.gov)