• The Food and Drug Administration (FDA) has listed P. putida strain KT2440 as Host-vector system safety level 1 certified (HV-1), indicating that it is safe to use without any extra precautions. (wikipedia.org)
  • The P. putida KT2440 chromosome is characterized by strand symmetry and intrastrand parity of complementary oligonucleotides. (wikipedia.org)
  • Phylogenomic analysis reclassified the strain KT2440 in a new species Pseudomonas alloputida. (wikipedia.org)
  • For this purpose, the non-pathogenic production host P. putida KT2440 containing the rhlAB genes with the native P. aeruginosa 5′-UTR region was used. (springer.com)
  • P. putida KT2440 has a versatile metabolism and high resistance towards oxidative stress. (rwth-aachen.de)
  • This thesis aimed to increase the number of rationally designed and well-characterized genetic tools for P. putida KT2440 to foster synthetic biology. (rwth-aachen.de)
  • Stable genomic integration is often used for metabolic engineering, but characterized sites, so-called landing pads, across the genome of P. putida KT2440 are missing. (rwth-aachen.de)
  • We analyzed RNA-Seq data towards regions that are equally expressed to identify suitable integration sites across the genome of P. putida KT2440. (rwth-aachen.de)
  • In summary, this thesis contributes to P. putida KT2440 synthetic biology. (rwth-aachen.de)
  • Getting the tough challenges, like the use of aromatic compounds from plastic or lignin as substrates, will distinguish P. putida KT2440 from the well-established microbes. (rwth-aachen.de)
  • Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory. (helmholtz-hzi.de)
  • Pseudomonas putida strain KT2440 is a versatile biosafety strain known for good expression of heterologous genes, so we have explored its utility as a cell factory for production of scFvs. (helmholtz-hzi.de)
  • A promoter engineering-based strategy enhances polyhydroxyalkanoate production in Pseudomonas putida KT2440. (helmholtz-hzi.de)
  • Southern hybridization experiments and analysis of the P. putida KT2440 genome sequence indicated that sepR is a relatively rare commodity compared to homologues of the sepABC genes. (canada.ca)
  • Pseudomonas putida KT2440 je bezpečná, robustní, původem půdní bakterie, nejdůkladněji prozkoumaná pseudomonáda známá vysokou odolností ke stresům. (muni.cz)
  • V navrhovaném projektu využijeme nejnovější nástroje a postupy metabolického inženýrství a syntetické biologie pro bezprecedentní přestavbu katabolismu sacharidů v kmeni P. putida EM42, což je P. putida KT2440 s redukovaným genomem a lepšími fyziologickými vlastnostmi. (muni.cz)
  • Thus, use of P. putida in many research labs is preferable to some other Pseudomonas species, such as Pseudomonas aeruginosa, for example, which is an opportunistic pathogen. (wikipedia.org)
  • Aztreonam resistance could not be transferred by conjugation between IMP-1-producing (aztreonam-resistant) P . putida and P . aeruginosa ( 2 ) and is not associated with a transposon carrying blaVIM-2 ( 6 ). (cdc.gov)
  • Today, mainly heterologous production systems are targeted due to the advantage of non-pathogenic hosts as well as uncoupling from complex quorum sensing regulatory networks compared to their natural producer Pseudomonas aeruginosa . (springer.com)
  • Home Vol 11 Issue 2 Comparative Effect of Pseudomonas aeruginosa, Pseudomonas fluorescens and Pseudomonas putida on the. (microbiologyjournal.org)
  • Pseudomonas aeruginosa C , Pseudomonas fluorescens M and Pseudomonas putida L were selected for field studies. (microbiologyjournal.org)
  • P. aeruginosa , P. fluorescens and P. putida in individual and consortial combination to act as biofertilizer which can become one of the most promising biotechnologies to solve apple replant disease and can also improve production with low inputs in fertilizer. (microbiologyjournal.org)
  • All the twelve isolates from apple rhizosphere were morphologically, physiologically and biochemically characterized so as to select the isolates belonging to P. aeruginosa, P. putida and P. fluorescens group. (microbiologyjournal.org)
  • Pseudomonas aeruginosa and other members of this group of gram-negative bacilli are opportunistic pathogens that frequently cause hospital-acquired infections, particularly in ventilator patients, burn patients, and patients with neutropenia or chronic debility. (msdmanuals.com)
  • In humans, P. aeruginosa is the most common pathogen, but infection may result from P. paucimobilis , P. putida , P. fluorescens , or P. acidovorans . (msdmanuals.com)
  • Here, synthetic biology is used for the tailoring of hyperproducing strains, starting with the prominent chassis strain Pseudomonas putida. (rwth-aachen.de)
  • In this study, we tested how a gradient of spatial habitat complexity in the form of fractal mazes influenced the growth, substrate degradation, and interactions of the bacterial strain Pseudomonas putida and the fungal strain Coprinopsis cinerea. (lu.se)
  • We used experimental evolution of the model environmental bacterium Pseudomonas putida to ask whether bacteria respond differently to Ag + or AgNP treatment. (birmingham.ac.uk)
  • In the absence of this treatment, bioassays with the bacterium Pseudomonas putida (ATCC11250) showed decreasing specific growth rates μ (used here to quantify 2,4-DCP toxicity) with increasing concentrations of 2,4-DCP. (njit.edu)
  • Bio-agents such as Hypocrea rufa (Trichoderma viride) and Pseudomonas fluorescens were also found effective in inhibiting the bacterial pathogen both in vitro and in vivo. (ijpcbs.com)
  • The aim of this work is to empower the anoxic metabolism of Pseudomonas putida to enable bioproduction anaerobically, with the redox power from a bioelectrochemical system (BES). (aiche.org)
  • A species-specific extragenic palindromic sequence is the most common repeat in the genome that can be exploited for the typing of P. putida strains. (wikipedia.org)
  • Type strains of Pseudomonas are well-known to represent only a small fraction of this diversity and the number of available Pseudomonas genome sequences is increasing rapidly. (preprints.org)
  • Phylogenies based on the rpoD gene and whole genome sequences, including 316 and 313 type strains of Pseudomonas, respectively, re-vealed sixteen groups of Pseudomonas and justified the partitioning of the P. putida group into fifteen subgroups. (preprints.org)
  • Pairwise average nucleotide identities were calculated between type strains and a selection of 60 genomes of non-type strains of Pseudomonas. (preprints.org)
  • Forty-one strains were incor-rectly assigned at the species level and among those, 19 strains were shown to represent an addi-tional 13 new Pseudomonas species that remain to be formally classified. (preprints.org)
  • The main objective of this research was to exploit the indigenous potential fluorescent Pseudomonas strains viz. (microbiologyjournal.org)
  • Based on 16S rRNA analysis, P. putida was taxonomically confirmed to be a Pseudomonas species (sensu stricto) and placed, along with several other species, in the P. putida group, to which it lends its name. (wikipedia.org)
  • Consequently, new Pseudomonas species are regularly reported and the number of species within the genus is in constant evolution. (preprints.org)
  • In this study, whole genome se-quencing enabled us to define 43 new Pseudomonas species and to provide an update of the Pseu-domonas evolutionary and taxonomic relationships. (preprints.org)
  • Fluorescent Pseudomonas species have a number of traits like antifungal, siderophores, phosphatesolubilization, lytic enzymes, HCN, ammonia and plant growth regulators that allow them to act as plant growth promoting and biocontrol agents. (microbiologyjournal.org)
  • Fluorescent Pseudomonas species are the most diverse and versatile group of plant growth promoting rhizobacteria. (microbiologyjournal.org)
  • P. putida ATH-43 genome now forms part of the 65 genomes of this species registered at the NCBI database (September, 2016) and it is highly related with the endophytic strain P. putida W619, which is also resistant to several heavy metals. (unab.cl)
  • Pseudomonas putida has demonstrated potential biocontrol properties, as an effective antagonist of plant pathogens such as Pythium aphanidermatum and Fusarium oxysporum f.sp. (wikipedia.org)
  • The protein count and GC content of the (63) genomes that belong to the P. putida wider evolutionary group (as defined by a phylogenomic analysis of 494 complete genomes from the entire Pseudomonas genus) ranges between 3748-6780 (average: 5197) and between 58.7-64.4% (average: 62.3%), respectively. (wikipedia.org)
  • The core proteome of the analyzed 63 genomes (of the P. putida group) comprised 1724 proteins, of which only 1 core protein was specific for this group, meaning that it was absent in all other analyzed Pseudomonads. (wikipedia.org)
  • Enhanced annotations and features for comparing thousands of Pseudomonas genomes in the Pseudomonas genome database. (pseudomonas.com)
  • Pseudomonas putida's amenability to genetic manipulation has allowed it to be used in the synthesis of numerous organic pharmaceutical and agricultural compounds from various substrates. (wikipedia.org)
  • Upgrading Pseudomonas putida by synthetic biology for biotechnological processing of lignocellulosic substrates. (muni.cz)
  • The pathogen responsible for the disease was isolated and identified as Pseudomonas putida based biochemical and molecular methods. (ijpcbs.com)
  • The serial dilution agar plate method was used to isolate Pseudomonas sp. (microbiologyjournal.org)
  • Characterization of a novel blaIMP gene, blaIMP-58, using whole genome sequencing in a Pseudomonas putida isolate detected in Denmark. (cdc.gov)
  • In this report we present the first draft genome sequence of a P. putida strain isolated from the Antarctic continent. (unab.cl)
  • To the best of our knowledge, this is the first report of the occurrence of bacterial blight disease of Chrysanthemum caused by Pseudomonas putida in Karnataka, India. (ijpcbs.com)
  • An R-prime, pUU2, derived from the broad host-range plasmid R68.44+ was generated carrying the dehalogenase I gene from Pseudomonas putida PP3. (warwick.ac.uk)
  • A new gene cluster, designated sepABC and a divergently transcribed sepR, was found downstream of the two-component todST phosphorelay system that regulates toluene degradation (the tod pathway) in Pseudomonas putida F1 (PpF1). (canada.ca)
  • The bacterium P. putida is being engineered for the conversion of lignin-derived monomers into key chemicals, such as bioplastic or Nylon precursors. (lu.se)
  • For example, we have developed a modified P. putida strain that can convert Kraft-lignin derived guaiacol into muconic acid, a precursor for Nylon 66 ( https://pubs.acs.org/doi/full/10.1021/acssuschemeng.1c00933 ). (lu.se)
  • We sought to better understand the IAA biosynthesis pathways operating in Pseudomonas putida UW4 by isolating, purifying and characterizing some of the implicated enzymes. (uwaterloo.ca)
  • We performed a random mutagenesis screen of the unique IutY protein of Pseudomonas putida, a protein that combines a cytosolic σECF domain and a periplasmic anti-sigma factor domain in a single polypeptide. (vu.nl)
  • Pseudomonas putida UW4 is a rhizospheric plant-growth-promoting bacterium that has the ability to produce IAA. (uwaterloo.ca)
  • The large scale application of indigenous plant growth promoting fluorescent Pseudomonas sp. (microbiologyjournal.org)
  • Pseudomonas putida colonies, in triplicate, were exposed to the test substance (directly diluted in test medium) at nominal concentrations of 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L. Inhibition of cell growth compared to controls was recorded after 16 h. (europa.eu)
  • A variety of P. putida, called multiplasmid hydrocarbon-degrading Pseudomonas, is the first patented organism in the world. (wikipedia.org)
  • P. putida was able to survive and produce almost exclusively 2‑Keto-gluconate from glucose under anoxic conditions due to redox balancing with electron mediators in a BES. (aiche.org)
  • The present study portrays the synthesis of mcl PHAs from Pseudomonas putida using glucose as the sole carbon source. (envirobiotechjournals.com)
  • P. putida cells survived under anaerobic conditions and limited biofilm formation could be observed on the anode surface. (aiche.org)
  • However, carbapenem-susceptible P . putida had low levels of susceptibility because the MIC 50 was only 1 dilution below the current breakpoint ( 7 , 8 ). (cdc.gov)
  • This work pinpoints the importance of correct taxonomic assignment and phylogenetic classification in order to perform integrative studies linking genetic diversity, lifestyle and metabolic potential of Pseudomonas spp. (preprints.org)
  • Production and characterization of a recombinant single-chain antibody (scFv) for tracing the σ54 factor of Pseudomonas putida. (helmholtz-hzi.de)
  • L-Methionine γ-lyase from Pseudomonas putida has a conserved tyrosine residue (Tyr114) in the active site as in all known sequences of γ-family pyridoxal 5′-phosphate dependent enzymes. (elsevierpure.com)
  • Pseudomonas putida is a promising host for the bioproduction of chemicals, but its industrial applications are significantly limited by its obligate aerobic character. (aiche.org)
  • Pseudomonas putida CBB5, a nonengineered, wild-type variety found in soil, can live on caffeine and has been observed to break caffeine down into carbon dioxide and ammonia. (wikipedia.org)
  • The purpose of this research is to determine whether a plant-associated strain o Pseudomonas putida has the ability to produce cyanide under biofilm conditions. (andrews.edu)