• Complete genome sequence of Pseudomonas aeruginosa lytic bacteriophage PA1O which resembles temperate bacteriophage D3112. (nih.gov)
  • Uchiyama J, Rashel M, Takemura I, Kato S, Ujihara T et al (2012) Genetic characterization of Pseudomonas aeruginosa bacteriophage KPP10. (springer.com)
  • Drew RE, Clarke PH, Brammar WJ (1980) The construction in vitro of derivatives of bacteriophage lambda carrying the amidase genes of Pseudomonas aeruginosa . (springer.com)
  • Krylov VN, Bogush VG, Shapiro JA (1980a) Bacteriophage of Pseudomonas aeruginosa with DNA similar in structure to that of phage Mul. (springer.com)
  • Krylov VN, Plotnikova TG, Kulakov LA, Fedorova TV, Eremenko EN (1982) Integration of genome of Mu-like bacteriophage D3112 of Pseudomonas aeruginosa into plasmid RP4 and its introduction within the hybrid plasmid into Pseudomonas putida and Escherichia coli C600 cells. (springer.com)
  • Genome, Proteome and Structure of a T7-Like Bacteriophage of the Kiwifruit Canker Phytopathogen Pseudomonas syringae pv. (mdpi.com)
  • An alternative or supplement to antibiotic therapy, which is currently being reexamined, is the use of bacterial viruses (phage/bacteriophage) to target bacterial infections, i.e., phage therapy ( 13 , 16 - 18 , 22 , 29 , 30 - 32 ). (asm.org)
  • In this study, SCVs was derived from Pseudomonas aeruginosa exposed to the lytic bacteriophage PB1 and these cells were resistant to subsequent phage infection by PB1. (semanticscholar.org)
  • Challenges and Promises for Planning Future Clinical Research Into Bacteriophage Therapy Against Pseudomonas aeruginosa in Cystic Fibrosis. (semanticscholar.org)
  • Bacteriophage therapy for refractory Pseudomonas aeruginosa urinary tract infection. (semanticscholar.org)
  • Bacterial resistance to bacteriophage is principally mediated by modification of the phage receptors although other mechanisms can play a role by blocking the viral entry and multiplication [2]. (chromoscience.com)
  • Structural modifications: mutating or modifying global elements of cell to adapt to Antibiotic (Such as increased acid tolerance to an acidic antimicrobial) Bacteriophage therapy, commonly known as 'phage therapy,' uses bacteria-specific viruses to kill antibiotic resistant bacteria. (wikipedia.org)
  • Walmagh M, Briers Y, dos Santos SB, Azeredo J, Lavigne R. Characterization of modular bacteriophage endolysins from Myoviridae phages OBP, 201φ2-1 and PVP-SE1. (ugent.be)
  • They found that when they cultured the bacterial strain Pseudomonas phaseolicola HB1OY with wild Pseudomonas phaseolicola from infected bean straw, there was cytopathic effect, implying that there was a lytic bacteriophage present in their wild Pseudomonas. (wikipedia.org)
  • Type IV pili of the opportunistic pathogen Pseudomonas aeruginosa mediate twitching motility and act as receptors for bacteriophage infection. (asm.org)
  • Any of various gram-negative, rod-shaped, mostly aerobic flagellated bacteria of the genus Pseudomonas, commonly found in soil, water, and decaying matter, and including some plant and animal pathogens. (thefreedictionary.com)
  • The burn wound pathology, combined to a global intensive use of antibiotics (ATB), put patients at high risk of suffering from multidrug resistant (MDR) infections, notably carried out by the Gram-negative bacteria species: Pseudomonas aeruginosa or Escherichia coli. (europa.eu)
  • Some phages only slightly differing in DNA restriction pattern from ϕKZ may be used to study the origin of ϕKZ genes coding for orthologs of proteins of unrelated species (other phages, pathogenic bacteria, eukaryotes). (deepdyve.com)
  • This phage is similar in size and structure to the previously described and serologically related phage B3 (Holloway, Egan & Monk, 1960), but differs from it in its plating properties on bacteria harbouring R plasmids. (microbiologyresearch.org)
  • Phage therapy predicted advantages are to target only specific bacteria, to adapt to those resistant and to penetrate inside bacterial biofilms. (cf-europe.eu)
  • To address the kinetics of infection, larvae were bled over a period of 24 h for quantification of bacteria and phages. (cardiffmet.ac.uk)
  • Five different bacteria] promoters were experimentally identified using a promoter trap assay, three of which have a sigma(54)-specific binding site and regulate transcription in the genome region involved in phage particle formation and host lysis. (uhasselt.be)
  • Pseudomonas fluorescens is a physiologically diverse species of bacteria present in many habitats, which possesses multifunctional traits that provide it with the capability to exhibit biological cont. (bioportfolio.com)
  • Competitive interaction on dual-species biofilm formation by spoilage bacteria, Shewanella baltica and Pseudomonas fluorescens. (bioportfolio.com)
  • Multiple mechanisms are used by bacteria to resist phages in a trade-off between survival and maintenance of fitness. (chromoscience.com)
  • Long-term phage/bacteria arm-race has been extensively investigated in the laboratory, mostly using coevolution in chemostats, and a few studies have observed coevolution in natural environment [6,7]. (chromoscience.com)
  • In the environment, many factors influence the fate of bacteria and phage variants by affecting the fitness as observed with Pseudomonas fluorescens for example [8]. (chromoscience.com)
  • Another actor in phage/bacteria evolution is the RecA-dependent spontaneous prophage induction (SPI) that influence bacterial fitness [14]. (chromoscience.com)
  • During maintenance of the phage a small proportion of bacteria can support a full multiplication cycle and die releasing new phages, whereas some cells maintain small amounts of viral genome and other are cured, becoming prey for the free phages. (chromoscience.com)
  • Phages are virulent, lyse Pseudomonas bacteria, and are characterized by very large heads and correspondingly high DNA contents. (nih.gov)
  • Examples of MDR bacteria identified as serious threats to public health include: Gram-positive MDR bacteria Clostridioides difficile Staphylococcus aureus Streptococcus pneumoniae Gram-negative MDR bacteria Carbapenem-resistant Acinetobacter Escherichia coli Klebsiella pneumoniae Neisseria gonorrhoeae Campylobacter Pseudomonas aeruginosa Salmonella Shigella Other MDR bacteria Mycobacterium tuberculosis MDR bacteria employ a plurality of adaptations to overcome the environmental insults caused by antibiotics. (wikipedia.org)
  • Phage therapy offers considerably higher specificity as the phage can be engineered to only infect a certain bacteria species. (wikipedia.org)
  • The generic name Pseudomonas created for these organisms was defined in rather vague terms by Walter Migula in 1894 and 1900 as a genus of Gram-negative, rod-shaped and polar- flagellated bacteria with some sporulating species, [3] [4] the latter statement was later proved incorrect and was due to refractive granules of reserve materials. (wikipedia.org)
  • This study supports the use of phage-encoded EPS depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials. (frontiersin.org)
  • Practiced in the United States until the 1940s and still used in parts of Eastern Europe, it pits tiny viruses-bacteriophages, or phages for short-against disease-causing bacteria. (scientificamerican.com)
  • Pseudomonas syringae pathovar phaseolicola bacteria serve as natural hosts. (wikipedia.org)
  • Pseudomonas bacteria serve as the natural host. (wikipedia.org)
  • It is the first to explore how filamentous phages, which are stringy bacteria-attacking viruses, can contribute to lung disease. (stanford.edu)
  • The new study's findings contradict this assumption: CF patients with phage-infected bacteria in their lungs fared worse than those with uninfected lung bacteria. (stanford.edu)
  • We saw that phage infection of the lung bacteria is associated with more antibiotic resistance in patients," Burgener said. (stanford.edu)
  • rather, the still-living bacteria incorporate the phage DNA into their own DNA and begin churning out lots of viral particles. (stanford.edu)
  • The researchers looked at genetic analyses of Pseudomonas bacteria from the lungs of 34 CF patients in Denmark. (stanford.edu)
  • Patients were more likely to develop consistent phage infections as they got older, supporting the idea that the virus-infected bacteria come to dominate CF patients' lungs over time. (stanford.edu)
  • The phage exhibited a wide range of thermal and pH stability and demonstrated considerable adsorption to host bacteria in the presence of CaCl 2 or MgCl 2 . (springer.com)
  • These results suggest the potential of applying phages, especially phage cocktails, to the surfaces of indwelling medical devices for mitigating biofilm formation by clinically relevant bacteria. (asm.org)
  • Phages can also be used in the clinical setting as tools for detecting specific bacteria from patient samples and for the rapid identification of antibiotic-resistant bacterial strains. (asmscience.org)
  • They work by injecting their DNA inside bacterial cells, where they replicate wildly, produce new phages, and eventually cause the bacteria to rupture - releasing even more viruses to hunt for bacterial prey. (buzzfeed.com)
  • Phages' power comes from two places: They are the most common and diverse organisms on Earth, and each phage harms only specific strains of bacteria. (buzzfeed.com)
  • Doctors have been using phages to kill bacteria for nearly a century - just not in the U.S. (buzzfeed.com)
  • They are assembled through the general secretion pathway and are expressed in a divergent collection of gram-negative bacteria, including Pseudomonas aeruginosa , Neisseria gonorrhoeae , Neisseria meningitidis , Moraxella bovis , Eikenella corrodens , Vibrio cholerae , the cyanobacterium Synechocystis sp. (asm.org)
  • The way this giant [ Pseudomonas ] phage replicates inside bacteria is so fascinating," said Vorrapon Chaikeeratisak of UC San Diego. (icr.org)
  • Phages are viruses that invade and multiply in bacteria, finally causing them to lyse, or burst. (fraunhofer.de)
  • The great advantage of phages is that they attack only a specific host bacterium, ignoring bodily cells and other bacteria. (fraunhofer.de)
  • Once a certain cell density has been attained, we add phages that invade the cells and continue to propagate there until all bacteria are destroyed. (fraunhofer.de)
  • Bacteriophages (phages), viruses that infect bacteria, may be one of such factors. (usda.gov)
  • This study explored the abundance, diversity, and functional role of phages infecting Gram-negative bacteria in a commercial cucumber fermentation. (usda.gov)
  • On day 1 and day 3 of the fermentation, 39 Gram-negative bacteria and 26 independent phages were isolated. (usda.gov)
  • This study revealed the abundance and variety of phages infecting Gram-negative bacteria, particularly Enterobacteriaceae, in the commercial cucumber fermentation, suggesting that phages may play an important role in the elimination of Gram-negative bacteria, thereby facilitating the dominance of LAB and minimizing bloater defect. (usda.gov)
  • To our knowledge, this is the first report on the ecology of phages infecting Gram-negative bacteria in commercial cucumber fermentations. (usda.gov)
  • Thomas JA, Rolando MR, Carroll CA, Shen PS, Belnap DM et al (2008) Characterization of Pseudomonas chlororaphis myovirus 201varphi2-1 via genomic sequencing, mass spectrometry, and electron microscopy. (springer.com)
  • Based on this, we recommend genome sequence analysis as an early step to select candidate phages for potential application in phage-therapy, before entering into a more intensive characterization. (mdpi.com)
  • Bioinformatics analysis of genomic data enables an in-depth characterization of phages and the choice of phages. (biomedcentral.com)
  • Only whole genome sequencing (WGS) enable an in-depth characterization of phages and the choice of the phages suitable for phage therapy. (biomedcentral.com)
  • Characterization of 83 site-directed mutants of Wzy from Pseudomonas aeruginosa PAO1 (Wzy Pa ) in topologically-mapped periplasmic (PL) and cytoplasmic loops (CL) verified the functional importance of PL3 and PL5, with the former shown to require overall cationic properties. (nature.com)
  • Here we describe the in depth characterization of a broad host range PB1-like phage with a slight prevalence to clinical isolates. (biomedcentral.com)
  • Characterization of multicopper oxidase CopA from Pseudomonas putida KT2440 and Pseudomonas fluorescens Pf-5: Involvement in bacterial lignin oxidation. (bioportfolio.com)
  • Ackermann H-W (2009) Phage classification and characterization. (springer.com)
  • In the context of a worldwide growing antibiotic resistance threat, notably the emergence of multi-drug resistant, even pan-resistant, bacterial strains, PhagoBurn was launched to evaluate the clinical potential of bacteriophages (phages) as a novel and innovative strategy to fight this critical issue. (europa.eu)
  • One of the approaches to the treatment of infections caused by multiply-(MDR) or extra drug- resistant (XDR) pathogenic strains may be application of bacterial viruses (bacteriophages)- phage therapy. (scirp.org)
  • We isolated and characterized a phage, named JG024, which infects a broad range of clinical and environmental P. aeruginosa strains. (biomedcentral.com)
  • The Galleria mellonella infection model was used to assess the in vivo efficacy of phage therapy against laboratory and clinical strains of Pseudomonas aeruginosa. (cardiffmet.ac.uk)
  • In a second series of experiments using clinical strains to further validate the prevention model, phages protected Galleria when infected with both a bacteraemia (0% vs. 85%) and a cystic fibrosis (80% vs. 100%) isolate. (cardiffmet.ac.uk)
  • Aims: The aim of this study was to determine whether manuka honey affected siderophore production by three strains of Pseudomonas aeruginosa. (cardiffmet.ac.uk)
  • All of these studies have, until now, been performed in non-functional Pseudomonas host strains which lack twitching motility. (nih.gov)
  • [7] As a result, the genus Pseudomonas includes strains formerly classified in the genera Chryseomonas and Flavimonas . (wikipedia.org)
  • [8] Other strains previously classified in the genus Pseudomonas are now classified in the genera Burkholderia and Ralstonia . (wikipedia.org)
  • By 2016, more than 400 strains of Pseudomonas had been sequenced. (wikipedia.org)
  • They inoculated confluent monolayers with two lux - tagged Pseudomonas strains, one mucoid, the other non-mucoid. (asmblog.org)
  • Dr. Nighat, Railway General Hospital, Pakistan, provided bacterial strains to establish host range of phage MJ2. (springer.com)
  • Capra M, Quiberoni A, Reinheimer J (2006) Phages of Lactobacillus casei/paracasei: response to environmental factors and interaction with collection and commercial strains. (springer.com)
  • During the lytic replication cycle, the infection of a bacterial host cell by a single phage virion will result in the production of dozens or hundreds of progeny phage, depending on the particular phage and host strains. (asm.org)
  • These observations, in addition to electron microscopy studies comparing phage binding of pilT mutant and wild-type strains ( 9 ), suggested that PilB enables pili extension whereas PilT and PilU catalyze pili retraction ( 15 , 23 ). (asm.org)
  • Infection of strain PAO1 with phage D3112 results in a 40- to 65-fold increase in the frequency of ami mutants resistant to fluoroacetamide. (springer.com)
  • Mice compromised by a burn wound injury and subjected to a fatal infection with Pseudomonas aeruginosa were administered a single dose of a Pseudomonas aeruginosa phage cocktail consisting of three different P. aeruginosa phages by three different routes: the intramuscular (i.m.), subcutaneous (s.c.), or intraperitoneal (i.p.) route. (asm.org)
  • This study models phage induction, cross infection, phage genome comparison and twitching studies linking the results to clinical data. (northumbria.ac.uk)
  • The data describes how phages derived from PA in older CF patients are: (i) highly adapted to infect PA that can colonise the lung, (ii) have higher rates of self-infection, (iii) have distinct infection profiles that prescribe the bacterial origin of the phage and (iv) how the addition of phage encoded genetic traits offer positive selection for their bacterial host. (northumbria.ac.uk)
  • Pseudomonas aeruginosa causes lung infections in patients suffering from the genetic disorder Cystic Fibrosis (CF). Once a chronic lung infection is established, P. aeruginosa cannot be eradicated by antibiotic treatment. (biomedcentral.com)
  • However, little is known about the factors which influence phage infection of P. aeruginosa under infection conditions and suitable broad host range phages. (biomedcentral.com)
  • We used an artificial sputum medium to study phage infection under conditions similar to a chronic lung infection. (biomedcentral.com)
  • Phage infection experiments under simulated chronic lung infection conditions showed that alginate production reduces phage infection efficiency. (biomedcentral.com)
  • In a first series of experiments, Galleria were infected with the laboratory strain P. aeruginosa PAO1 and were treated with varying multiplicity of infection (MOI) of phages either 2 h post-infection (treatment) or 2 h pre-infection (prevention) via injection into the haemolymph. (cardiffmet.ac.uk)
  • By 24 h post-infection, phages kept bacterial cell numbers in the haemolymph 1000-fold lower than in the non-treated group. (cardiffmet.ac.uk)
  • This study will describe clinical outcomes in patients who received ceftolozane-tazobactam for a Pseudomonas aeruginosa infection. (bioportfolio.com)
  • We analyse here the mutations emerging in a clinical Pseudomonas aeruginosa strain in response to infection by Ab09, a N4-like lytic podovirus and describe a variety of chromosomal deletions and mutations conferring resistance. (chromoscience.com)
  • One major drawback to phage therapy is the evolution of phage-resistant microbes which was seen in a majority of phage therapy experiments aimed to treat sepsis and intestinal infection. (wikipedia.org)
  • During lytic infection, transcriptional analysis found evidence of phage-host interaction through the strong upregulation of the nrdR nucleotide biosynthesis regulon. (asm.org)
  • We think the virus is helping Pseudomonas to establish chronic infection in CF patients' lungs and potentially making patients sicker over time. (stanford.edu)
  • The team tested the sputum for genetic signatures from Pseudomonas and filamentous phages and found that 58 people had Pseudomonas infection. (stanford.edu)
  • Current Research and Scholarly Interests I am currently studying filamentous phage in Pseudomonas infection in cystic fibrosis. (stanford.edu)
  • Phages can replicate at the site of an infection, thereby increasing in numbers where they are most required. (asm.org)
  • At least in theory, a single phage dose should be capable of treating a biofilm infection as progeny phage infect adjacent cells and degrade the biofilm matrix. (asm.org)
  • Nearly 67% of Gram-negative bacterial isolates were susceptible to phage infection. (usda.gov)
  • The type species is Pseudomonas aeruginosa . (thefreedictionary.com)
  • Biebl, M. 2004-10-18 00:00:00 To study the genome diversity of bacteriophages from geographically distant natural populations, new giant ϕKZ-like Pseudomonas aeruginosa phages isolated in two different regions were compared with earlier known phages of three species (ϕKZ, Lin68, EL). (deepdyve.com)
  • Phages of the ϕKZ species proved to be common in natural populations of various regions, while EL- and Lin68-related phages were extremely rare. (deepdyve.com)
  • The spectrum of capsid polypeptides proved to be conserved in each species, and was proposed as a character necessary and sufficient for express classification of phages with an accuracy of species. (deepdyve.com)
  • 13] Krylov, V.N., Tolmachova, T.O. and Akhverdian, V.Z. (1993) DNA Homology in Species of Bacteriophages Active on Pseudomonas aeruginosa. (scirp.org)
  • This study aims to characterize the biofilm produced by mono- and dual-species of Shewanella baltica and Pseudomonas fluorescens as fish spoilers at the different incubation temperature, and to elucid. (bioportfolio.com)
  • N4-like phages are podoviruses distributed into four genera frequently isolated from different Gram-negative species and characterized by the presence of a giant virion-associated RNA polymerase [34,35,36]. (chromoscience.com)
  • The phiKZ genus has very few relationships to other phages and includes three species and one possible species. (nih.gov)
  • Pseudomonas is a genus of Gram-negative , Gammaproteobacteria , belonging to the family Pseudomonadaceae and containing 191 validly described species. (wikipedia.org)
  • [5] Despite the vague description, the type species, Pseudomonas pyocyanea (basonym of Pseudomonas aeruginosa ), proved the best descriptor. (wikipedia.org)
  • Other characteristics that tend to be associated with Pseudomonas species (with some exceptions) include secretion of pyoverdine , a fluorescent yellow-green siderophore [13] under iron-limiting conditions. (wikipedia.org)
  • [15] Pseudomonas species also typically give a positive result to the oxidase test , the absence of gas formation from glucose, glucose is oxidised in oxidation/fermentation test using Hugh and Leifson O/F test, beta hemolytic (on blood agar ), indole negative, methyl red negative, Voges-Proskauer test negative, and citrate positive. (wikipedia.org)
  • Approximately half of all bacterial species encode CRISPR-Cas adaptive immune systems1, which provide immunological memory by inserting short DNA sequences from phage and other parasitic DNA elements into CRISPR loci on the host genome2. (exeter.ac.uk)
  • Whereas CRISPR loci evolve rapidly in natural environments3, bacterial species typically evolve phage resistance by the mutation or loss of phage receptors under laboratory conditions4,5. (exeter.ac.uk)
  • OBPgp279, on the other hand, is suggested to intrinsically destabilize the outer membrane of Pseudomonas species, thereby gaining access to their peptidoglycan and exerts an antibacterial activity of 1 logarithmic unit reduction. (ugent.be)
  • There is currently only one species in this genus: the type species Pseudomonas phage phi6. (wikipedia.org)
  • Most identified cystoviruses infect Pseudomonas species, but this is likely biased due to the method of screening and enrichment. (wikipedia.org)
  • The type species is Pseudomonas phage phi6, but there are many other proposed members of this family. (wikipedia.org)
  • Scientists have struggled to understand how an aggressive bacterial species, Pseudomonas aeruginosa, persists in the lungs of CF patients who are receiving antibiotics, she added. (stanford.edu)
  • Multiple Pseudomonas species were also detected in tissue biopsy specimens. (asm.org)
  • They are not only required for microbial adherence but also involved in bacterial movement, such as social gliding motility in Myxococcus xanthus and twitching motility in Pseudomonas and Neisseria species ( 33 ). (asm.org)
  • About 88% of isolated phages infected the members in the family Enterobacteriaceae and 62% of phages infected Enterobacter species. (usda.gov)
  • We isolated a Pseudomonas phage infecting Pseudomonas fluorescens SA1 separated from a soil sample collected in Sichuan Province, China. (springer.com)
  • Nowicki G, Walkowiak-Nowicka K, Zemleduch-Barylska A, Mleczko A, Frackowiak P et al (2017) Complete genome sequences of two novel autographiviruses infecting a bacterium from the Pseudomonas fluorescens group. (springer.com)
  • Pseudomonas fluorescens phages from sewage were tested against P. fluorescens isolates of soil and sewage . (bvsalud.org)
  • The crystal structure of the CopC protein from Pseudomonas fluorescens reveals amended classifications for the CopC protein family. (bioportfolio.com)
  • protein from Pseudomonas fluorescens (Pf-CopC) that include the wild type protein bound to Cu(II) and two variant proteins, where Cu(II) coordinating ligands were mutated, in Cu-free states. (bioportfolio.com)
  • Response of Pseudomonas fluorescens to dimethyl phthalate. (bioportfolio.com)
  • Pseudomonas fluorescens (P. fluorescens) is one of the. (bioportfolio.com)
  • Coopting the Lap system of Pseudomonas fluorescens to reversibly customize bacterial cell surfaces. (bioportfolio.com)
  • Insights into the Draft Genome Sequence of the Kiwifruit-Associated Pathogenic Isolate Pseudomonas fluorescens AHK-1. (bioportfolio.com)
  • Endolysins OBPgp279 (from Pseudomonas fluorescens phage OBP. (ugent.be)
  • Endolysins OBPgp279 (from Pseudomonas fluorescens phage OBP), PVP-SE1gp146 (Salmonella enterica serovar Enteritidis phage PVP-SE1) and 201 phi 2-1gp229 (Pseudomonas chlororaphis phage 201 phi 2-1) all possess a modular structure with an N-terminal cell wall binding domain and a C-terminal catalytic domain, a unique property for endolysins with a Gram-negative background. (ugent.be)
  • 1995). Earthworm activities may also influence the rate of transfer of conjugative plasmids from Pseudomonas fluorescens to autochthonous soil microorganisms (DAANE et al. (sfsu.edu)
  • However, given the role of phages in the evolution of bacterial pathogens, it is necessary to discuss and evaluate negative consequences of mass introduction of phage therapy, as the measures necessary for its safe use. (scirp.org)
  • Therefore, this study validates the use of G. mellonella as a simple, robust and cost-effective model for initial in vivo examination of P. aeruginosa-targeted phage therapy, which may be applied to other pathogens with similarly low infective doses. (cardiffmet.ac.uk)
  • Phage therapy also allows for the possibility of biofilm penetration in cases where antibiotics are ineffective due to the increased resistance of biofilm-forming pathogens. (wikipedia.org)
  • Specifically, using the opportunistic pathogen Pseudomonas aeruginosa and its phage DMS3vir, we show that coexistence with other human pathogens amplifies the fitness trade-offs associated with phage receptor mutation, and therefore tips the balance in favour of CRISPR-based resistance evolution. (exeter.ac.uk)
  • I will now summarize two recent papers that use intact phages to combat two important bacterial pathogens, both in vitro and in vivo . (asmblog.org)
  • Phages haven't been thought of as pathogens that affect humans," said lead author Elizabeth Burgener , MD, an instructor of pediatrics at Stanford. (stanford.edu)
  • Armata is developing and advancing a broad pipeline of natural and synthetic phage candidates, including clinical candidates for Pseudomonas aeruginosa , Staphylococcus aureus , and other pathogens. (prnewswire.com)
  • The main objective of the project is to assess the safety, effectiveness and pharmacodynamics of two therapeutic phage cocktails to treat either E. coli or P. aeruginosa burn wound infections. (europa.eu)
  • Pseudomonas aeruginosa plays a prominent role as an etiological agent of serious infections in patients with burn wounds. (asm.org)
  • His findings demonstrated that the phage-treated grafts were protected in six of seven cases, while untreated grafts failed uniformly, suggesting that phage might be useful for the prevention of P. aeruginosa infections in patients with burn wounds. (asm.org)
  • However, while multiple studies have demonstrated the benefits of phage therapy for a variety of bacterial infections in animal model systems ( 3 - 7 , 10 , 14 , 19 , 23 - 26 , 33 - 35 ), little documentation exists with regard to the treatment of burn wound infections ( 2 ). (asm.org)
  • In the study described here we used the mouse model of thermal injury ( 28 ) to examine the efficacy of phage therapy in abrogating fatal P. aeruginosa infections. (asm.org)
  • 6] Krylov, V., Shaburova, O., Krylov, S. and Pleteneva, E. (2013) A Genetic Approach for Development of New Therapeutic Phages to Fight Pseudomonas Aeruginosa in Wound Infections. (scirp.org)
  • Phage therapy is an alternative to treat these chronic P. aeruginosa infections. (biomedcentral.com)
  • Since eradication of chronic P. aeruginosa infections by antibiotics fails, phage therapy is a possibility to treat bacterial infections. (biomedcentral.com)
  • Although manuka honey has been shown to inhibit Pseudomonas aeruginosa, an opportunistic pathogen implicated in cutaneous wound infections, the mechanisms of action are not yet defined. (cardiffmet.ac.uk)
  • Phage therapy involves the application of lytic bacteriophages for treatment of clinical infections but bacterial resistance may develop over time. (semanticscholar.org)
  • Bacteriophages for the treatment of Pseudomonas aeruginosa infections. (semanticscholar.org)
  • 2006) The Potential to Treat Chronic Dog-Ear Infections with Pseudomonas Specific Bacteriophages . (evergreen.edu)
  • The potential of aerosol phage therapy for treating lung infections has been demonstrated in animal models and clinical studies. (springer.com)
  • Hoiby N. Recent advances in the treatment of Pseudomonas aeruginosa infections in cystic fibrosis. (springer.com)
  • Antibiotics hadn't been discovered yet, so phages were a revolutionary weapon against all kinds of infections, Ryland Young, head of the Center for Phage Technology at Texas A&M University, told BuzzFeed News. (buzzfeed.com)
  • Type IV pili of Pseudomonas aeruginosa are significant adhesins for interaction with mammalian cells (reviewed in references 15 and 25 ), contributing to this organism's ability to cause opportunistic infections in humans. (asm.org)
  • Partners in this project, including the Fraunhofer Institute for Toxicology and Experimental Medicine ITEM at Braunschweig, aim to enlist bacterial viruses, also known as bacteriophages or simply phages, to wage war on bacterial infections. (fraunhofer.de)
  • The first item on their agenda is to develop an inhalable drug cocktail comprised of three bacteriophages to treat for the multidrug-resistant bacterium Pseudomonas aeruginosa, the most common bacterial cause of pneumonia in cystic fibrosis patients and urinary tract infections. (fraunhofer.de)
  • Phylogenetic and BLASTn analysis revealed that the Pseudomonas phage PPSC2 should be considered a new member of the family Myoviridae . (springer.com)
  • Accessed 27 Nov 2018], of which 19 are genome sequences of phages infecting Aeromonas (phages belonging to Myoviridae, Podoviridae, and Siphovirida e families) and 144 infecting Pseudomonas (phages belonging to Myoviridae, Podoviridae, Siphoviridae, Cystoviridae, Leviviridae, Inoviridae families and 5 unclassified). (biomedcentral.com)
  • PB1-like phages belong to the Myoviridae phage family and the genome sizes vary between 64,427 and 66,530 bp. (biomedcentral.com)
  • Several results of major significance for the future of phage therapy stemmed from this pioneer project. (europa.eu)
  • 7] Chan, B.K., Abedon, S.T. and Loc-Carrillo, C. (2013) Phage Cocktails and the Future of Phage Therapy. (scirp.org)
  • Are Phage Viruses the Forgotten Cure for Superbugs? (scientificamerican.com)
  • Environmental and clinical isolates of the bacterium Pseudomonas aeruginosa frequently contain viruses known as prophages. (asm.org)
  • Because phages infect only bacterial cells, scientists have assumed that the viruses do not act on human health. (stanford.edu)
  • abstract = "Pseudomonas aeruginose produces three types of bacteriocins: R-, F- and S-type pyocins. (elsevier.com)
  • Therefore, phages and especially the ability of the phage to infect the host in vivo should be investigated carefully prior to use. (biomedcentral.com)
  • Phage variants with mutations in a tail fiber gene are selected during pseudolysogeny with the capacity to infect resistant cells and produce large plaques. (chromoscience.com)
  • This phage, which we named PPSC2, has a genome that is composed of a 97,330-bp-long linear double-stranded DNA with 47.51% G+C content and 168 putative protein-coding genes. (springer.com)
  • This allows an optimised choice of phage for therapy, excluding those with toxin genes, virulence factor genes, and genes responsible for lysogeny. (biomedcentral.com)
  • From 11 newly isolated bacteriophages only 6 (25AhydR2PP, 50AhydR13PP, 60AhydR15PP, 22PfluR64PP, 67PfluR64PP, 71PfluR64PP) have a potential to be used in phage therapy due to confirmed lytic lifestyle and absence of virulence or resistance genes. (biomedcentral.com)
  • Despite this level of DNA homology throughout the genome, both phages combined have 15 unique genes that do not occur in the other phage. (uhasselt.be)
  • These exclusive genes encoded features related to metal and antibiotic resistance, phage integrase and transposons. (bioportfolio.com)
  • Finally this study points to the possible role of two bacterial genes in Ab09 phage adhesion to the cell, rpsB encoding protein S2 of the 30S ribosomal subunit and ORF1587 encoding a Wzy-like membrane protein involved in LPS biosynthesis. (chromoscience.com)
  • In fact, many genomes of Pseudomonas share only 50-60% of their genes, e.g. (wikipedia.org)
  • Although toxin genes are an important consideration, they are by no means the only virulence factor encoded by phages. (asmscience.org)
  • This protein is the prototype of the Pseudomonas phage F116 holin (F116 Holin) family (TC# 1.E.25), which is a member of the Holin Superfamily II. (wikipedia.org)
  • Of the seven phages isolated, one was found to be abundant in sewage (5.9×10(7) pfu/mL), having broad host range , and distinct protein and DNA profile when compared to the other six phages . (bvsalud.org)
  • DNA restriction and protein profiles of the phages and their morphology indicate the diversity in the sewage environment . (bvsalud.org)
  • CopA is a protein formed as part of a copper resistance operon in Pseudomonas syringae pv tomato, but CopA has also been identified from gene library screening as a potential lignin-oxidising enzyme. (bioportfolio.com)
  • The structure of uncharacterized protein PP-LUZ7_gp033 from Pseudomonas phage LUZ7. (proteopedia.org)
  • Group: dsRNA Order: Unassigned Family: Cystoviridae Genus: Cystovirus Pseudomonas phage phi6 Pseudomonas phage phi8 Pseudomonas phage phi12 Pseudomonas phage phi13 Pseudomonas phage phi2954 Pseudomonas phage phiNN Pseudomonas phage phiYY Cystoviruses are distinguished by their tripartite dsRNA genome, totaling ~14 kb in length and their protein and lipid outer layer. (wikipedia.org)
  • 3 There is a significant difference between bacterial and human cells, but amazingly the "phage convert the [bacterial] cells they have infected into mammalian-type cells with a centrally located nucleus-like structure, formed by a protein shell surrounding the replicated phage DNA. (icr.org)
  • Bioinformatics analysis of genomic data, assessing the likelihood of these phages to enter the lysogenic cycle with experimental data on their specificity towards large number of bacterial field isolates representing different locations. (biomedcentral.com)
  • None of the isolates from the rhizosphere regions of various cultivated soils were susceptible to phages isolated from sewage . (bvsalud.org)
  • Antimicrobial activity of ceftolozane-tazobactam against multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa clinical isolates from a Spanish hospital. (bioportfolio.com)
  • Biofilm isolates resistant to phage M4 were recovered from catheters pretreated with phage. (asm.org)
  • The phage susceptibility profiles of these isolates were used to guide the development of a five-phage cocktail from a larger library of P. aeruginosa phages. (asm.org)
  • 0.001), but fewer biofilm isolates were resistant to these phages. (asm.org)
  • The DSMZ then identified phages that are able to dissolve the isolates. (fraunhofer.de)
  • Pseudomonas phage phi6 was the first virus in this family to be discovered and was initially characterized in 1973 in James Van Etten's laboratory in the Department of Plant Pathology at the University of Nebraska. (wikipedia.org)
  • Van Belleghem J, Clement F, Merabishvili M, Lavigne R, Vaneechoutte M. Pro- and anti-inflammatory responses of peripheral blood mononuclear cells induced by Staphylococcus aureus and Pseudomonas aeruginosa phages. (ugent.be)
  • Curtin and Donlan ( 13 ) demonstrated that a phage that is active against Staphylococcus epidermidis could be incorporated into a hydrogel coating on a catheter and significantly reduce biofilm formation by this organism in an in vitro model system. (asm.org)
  • As we prepare to initiate trials of our Pseudomonas aeruginosa candidate, AP-PA02, and our Staphylococcus product candidate, AP-SA02, next year, Todd's guidance and insights will serve us well as we advance our pipeline while in parallel discovering and refining new phage-based therapeutics. (prnewswire.com)
  • A Pseudomonas podoviridae phage, PEV2, was incorporated into multi-component formulation systems consisting of trehalose, mannitol and L-leucine (F1 = 60:20:20 and F2 = 40:40:20). (springer.com)
  • All prophages have the same restriction map as the D3112 genome extracted from phage particles. (springer.com)
  • I. General description, localization of sites sensitive to endonucleases in DNA, and structure of homoduplexes of phage D3112. (springer.com)
  • Evidence of relationship to bacteriophages D3112, B3 and B39: analysis of cleavage of DNA by restriction endonucleases, isolation of recombinants of phages D3112 and B3. (springer.com)
  • Phages duplicate (replicate) within the bacterium following the injection of their genetic material (genome) through the bacteria's cell wall and into the bacterial cytoplasm. (icr.org)
  • Formation of biofilms under phage predation: considerations concerning a biofilm increase. (semanticscholar.org)
  • However, reports indicating that host populations can, in some cases, respond to phage predation by an increase in biofilm formation are of concern. (semanticscholar.org)
  • This study investigates whether phage predation can enhance the formation of biofilm and if so, if this phenomenon is governed by the emergence of phage-resistance or by non-evolutionary mechanisms (eg spatial refuge). (semanticscholar.org)
  • Numerous Pseudomonas cells were packed within the biofilm matrix and attached to the epithelial cells, as shown by staining with Calcofluor white , a fluorescence dye that binds to polysaccharides in biofilm matrices. (asmblog.org)
  • When the Pseudomonas cells were cultured for 24 hours in the presence of the phages, Calcofluor white staining showed only weak and open matrices, indicating considerable disruption of the biofilm architecture. (asmblog.org)
  • The log-phase growth of E. cloacae both in biofilm and suspension was significantly reduced by the phage. (springer.com)
  • The E. cloacae biofilm was formed under different conditions to evaluate the efficacy of MJ2 phage. (springer.com)
  • It was, therefore, concluded that phage MJ2 possessed considerable inhibitory effects on MDR E. cloacae both in planktonic and biofilm forms. (springer.com)
  • This study investigated the effect of pretreating hydrogel-coated catheters with Pseudomonas aeruginosa bacteriophages on biofilm formation by P. aeruginosa in an in vitro model. (asm.org)
  • Some phages also have been shown to produce enzymes that degrade the extracellular polymeric substance (EPS) matrix of a biofilm ( 23 , 25 ). (asm.org)
  • 19 ) showed that progeny phage will propagate radially through a biofilm. (asm.org)
  • Amgarten D, Martins LF, Lombardi KC, Antunes LP, de Souza APS et al (2017) Three novel Pseudomonas phages isolated from composting provide insights into the evolution and diversity of tailed phages. (springer.com)
  • Third, the PhagoBurn clinical study, representing the world first phage therapy clinical trial using GMP produced phage cocktails, was launched in July 2015 and ended in January 2017. (europa.eu)
  • The phages were characterized as to host range , morphology, structural proteins and genome fingerprint . (bvsalud.org)
  • Eighteen YuA proteins were identified as part of the phage particle by mass spectrometry analysis. (uhasselt.be)
  • Phage-induced alignment of membrane proteins enables the measurement and structural analysis of residual dipolar couplings with dipolar waves and lambda-maps. (cathdb.info)
  • The phage MJ2 was found to possess 11 structural proteins (12-150 kDa) and a double-stranded DNA genome with an approximate size of 40 kb. (springer.com)
  • Spatial structure affects phage efficacy in infecting dual-strain biofilms of Pseudomonas aeruginosa. (unil.ch)
  • A ) Growth of lux-tagged Pseudomonas biofilms on the surface of a CF-bronchial epithelia cell monolayer. (asmblog.org)
  • The researchers employed bioluminescence imaging to assess the ability of this phage mix to kill Pseudomonas cells in biofilms on the surface of a monolayer of a cystic fibrosis bronchial epithelial cell line. (asmblog.org)
  • Is the phage mixture effective against the Pseudomonas biofilms? (asmblog.org)
  • The use of phages to control biofilms has potential for several reasons. (asm.org)
  • The results of these studies indicated that a single dose of the P. aeruginosa phage cocktail could significantly decrease the mortality of thermally injured, P. aeruginosa -infected mice (from 6% survival without treatment to 22 to 87% survival with treatment) and that the route of administration was particularly important to the efficacy of the treatment, with the i.p. route providing the most significant (87%) protection. (asm.org)
  • A research team has recently investigated the efficacy of phage therapy in the treatment of CF. They isolated two phages (φMR299-2 and φNH-4) from sewage and showed that both were virulent for P. aeruginosa . (asmblog.org)
  • Earlier studies demonstrated the highly effective role of the innate immune system in the rapid removal of phages from the circulatory system, thereby decreasing the efficacy of intravenously injected phages. (asmscience.org)
  • 2] Cornelis, P. (Ed.) (2008) Pseudomonas: Genomics and Molecular Biology. (scirp.org)
  • Pseudomonas aeruginosa is one of the Multi-Drug-Resistant organisms most frequently isolated worldwide and, because of a shortage of new antibiotics, bacteriophages are considered an alternative for its treatment. (mdpi.com)
  • Phage therapy is garnering renewed interest as bacterial resistance to antibiotics becomes widespread. (cf-europe.eu)
  • When somebody first grows Pseudomonas, we try to eradicate it with antibiotics," Burgener said. (stanford.edu)
  • If approved by Congress, this money will go toward screening soil samples for new antibiotics, reducing the drugs in livestock feed, boosting hospital surveillance, and, perhaps, making phage therapy a viable option in the U.S. (buzzfeed.com)
  • But now, in light of antibiotic resistance, more and more research is focusing on phages - especially since no new antibiotics are being developed by the pharmaceutical industry," says Prof. Holger Ziehr, project manager at Fraunhofer ITEM. (fraunhofer.de)
  • Our goal is to develop phages as an additional therapeutic approach for a variety of infectious diseases, in particular those where antibiotics are no longer effective," says Ziehr. (fraunhofer.de)
  • Most ϕKZ-related phages had unique DNA restriction patterns, but the differences between these were only minor, and the genomes did not contain nonhomologous fragments. (deepdyve.com)
  • Transposable temperate phages randomly insert into bacterial genomes, providing increased supply and altered spectra of mutations available to selection, thus opening alternative evolutionary trajectories. (microbiologyresearch.org)
  • The Pseudomonas infecting PB1-like phages are widespread in nature and possess highly conserved genomes. (biomedcentral.com)
  • The patients had had their bacterial DNA sequenced repeatedly over time, allowing the researchers to see whether phage DNA had been persistently incorporated into the bacterial genomes. (stanford.edu)
  • The genome organization of both phages differs substantially from those of the other known Pseudomonas-infecting Siphoviridae, delineating them as a distinct genus within this family. (uhasselt.be)
  • In P. aeruginosa the corresponding genus "Lit1virus" includes Lit1, PA26 and Ab09 which display a rather large host spectrum, making them interesting tools for phage therapy. (chromoscience.com)
  • At the beginning of the project, no clear legal framework regulating the use of therapeutic phages was available in all EU member states but Poland, where the Helsinki treaty prevails at the Institute of Immunology and Experimental Therapy (Wroclaw) for using phage therapy. (europa.eu)
  • 17] Panagea, S., Winstanley, C., Walshaw, M.J., Ledson M.J. and Hart C.A. (2005) Environmental Contamination with an Epidemic Strain of Pseudomonas aeruginosa in a Liverpool Cystic Fibrosis Centre, and Study of Its Survival on Dry Surfaces. (scirp.org)
  • One of the important applications for phage therapy is for treating cystic fibrosis (CF). CF is an inherited genetic disorder where a defective enzyme results in the production of unusually viscous, sticky mucus and chloride-containing secretions in ducts and body cavities. (asmblog.org)
  • Govan JRW, Deretic V. Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia . (springer.com)
  • The holin gene of NgoPhi1 (identical to that encoded by NgoPhi2), when expressed in E. coli, could substitute for the phage lambda S gene. (wikipedia.org)
  • The YuA genome is neither Mu- nor lambda-like and encodes 78 gene products that cluster in three major regions involved in (i) DNA metabolism and replication, (ii) host interaction, and (iii) phage particle formation and host lysis. (uhasselt.be)
  • The results of independent experiments passing phage lambda through 10 successive cycles in mice were two mutant phages that were able to evade entrapment by the reticuloendothelium system. (asmscience.org)
  • We aimed to investigate the complete accessory genome of an ocular isolate of Pseudomonas aeruginosa strain PA34. (bioportfolio.com)
  • Staining confirms that P. aeruginosa strain NH57388A ( A ) and strain MR299 ( B ) are embedded in an exopolysaccharide structure prior to phage exposure. (asmblog.org)
  • Indeed, a dedicated committee of experts was quickly created by the ANSM to evaluate ATU requests and both were granted in France, one ATU for each phage cocktail. (europa.eu)
  • Most importantly, no serious adverse event was observed in the GMP phage cocktail arm, which is in line with the expected high level of safety for such treatments. (europa.eu)
  • The new phage cocktail will have to comply with European pharmaceutical requirements. (fraunhofer.de)
  • The idea here is to combine three different phages in a cocktail for the patient. (fraunhofer.de)
  • We would be able to heal around 70 of 100 patients with our phage cocktail," says Ziehr. (fraunhofer.de)
  • Once this extension will have been granted, the institute will be in a position to produce the three phages and mix them to a cocktail that will serve as investigational medicinal product for clinical trials. (fraunhofer.de)
  • They did this by culturing various leaves in Lysogeny Broth and then plating the broth on lawns of Pseudomonas syringae pv phaseolicola. (wikipedia.org)
  • Our objective was to evaluate the in vitro activity of ceftolozane-tazobactam against multidrug resistant (MDR) and extensively drug-resistant (XDR) non metallo-β-lactamase producing Pseudomonas aeru. (bioportfolio.com)
  • 2004) Comparison of in vitro and in vivo bacteriolytic activity of Escherichia coli phages: implications for phage therapy . (evergreen.edu)
  • The phage titer loss after the SFD and SD processes and in vitro aerosol performance of the produced powders were assessed. (springer.com)
  • In vitro aerosol performance showed that the SFD powders (~80% phage recovery) provided better phage protection than the SD powders (~20% phage recovery) during the aerosolization process. (springer.com)
  • Pseudomonas aeruginosa phage YuA (Siphoviridae) was isolated from a pond near Moscow, Russia. (uhasselt.be)
  • Flagellotropic bacteriophages belong to the tailed-phage order Caudovirales , the most abundant phages in the virome. (asm.org)
  • In Pseudomonas aeruginosa they are associated with a form of surface translocation known as twitching motility and have also been implicated as the receptor for a number of fimbrial-specific bacteriophages. (nih.gov)