The TCP receptor is a prophage receptor facilitating introduction of CTXφ prophage DNA into the cell 6 . (nature.com)
In V. cholerae , CTXφ prophage transmits toxin genes among the bacteria that express the TCP prophage receptor. (nature.com)
Prophage in Phage Manufacturing: Is the Risk Overrated Compared to Other Therapies or Food? (mdpi.com)
Genes5
Filamentous bacteriophages are among the simplest living organisms known, with far fewer genes than the classical tailed bacteriophages studied by the phage group in the mid-20th century. (wikipedia.org)
These proteins are the products of phage genes 3 and 6 at one end of the phage, and phage genes 7 and 9 at the other end. (wikipedia.org)
PMQR genes (including aac(6')-1b-cr, qnrB and qnrS) were also frequently detected. (afpm.org.my)
In Staphylococcus aureus, the phage-mediated acquisition of mobile genetic elements (MGEs) that encode virulence and antibiotic resistance genes largely contribute to its evolutionary adaptation and genetic plasticity. (bvsalud.org)
Here we show that staphylococcal phages produce a structured RNA transcribed from the terminase subunit genes, termed CBASS-activating bacteriophage RNA (cabRNA), which binds to a positively charged surface of the CdnE03 cyclase and promotes the synthesis of the cyclic dinucleotide cGAMP to activate the CBASS immune response. (bvsalud.org)
Escherichia2
Escherichia phage N4 was isolated in 1966 in Italy and has remained a genomic orphan for a long time. (mdpi.com)
The isolates were identified as Klebsiella pneumoniae (n=9), Escherichia coli (n=6), Enterobacter cloacae complex (n=5) and Enterobacter spp. (afpm.org.my)
Bacteriophage1
In this study, a Salmonella Typhimurium lytic bacteriophage, Φ st1, which was isolated from chicken faecal material, was evaluated as a candidate for biocontrol of Salmonella in chickens. (afpm.org.my)
Bacterium2
This distinctive shape reflects their method of replication: the coat of the virion comprises five types of viral protein, which are located in the inner membrane of the host bacterium during phage assembly, and these proteins are added to the nascent virion's DNA as it is extruded through the membrane. (wikipedia.org)
S. aureus is known to bind multiple coagulation factors, resulting in the formation of aggregates and blood clots that might protect the bacterium from the phages. (bvsalud.org)
Aureus5
Here we describe our studies on the genetic organization of phages infecting Staphylococcus aureus, a pathogen of human importance, by using an assembly of tools for gene annotation, identification of expression components, and phylogeny analysis. (bvsalud.org)
S. aureus is a pathogen that frequently causes severe morbidity and phage therapy is being discussed as an alternative to antibiotics for the treatment of S. aureus infections. (bvsalud.org)
Consistent with these in vitro findings, phage treatment did not reduce bacterial burdens in a neutropenic mouse S. aureus thigh infection model. (bvsalud.org)
In summary, phage treatment of S. aureus infections inside the body may be fundamentally challenging, and more investigation is needed prior to proceeding to in-human trials. (bvsalud.org)
however, few specimens of the so-called jumbo phages capable of infecting S. aureus have been described. (bvsalud.org)
Bacteria2
The two ends of the phage are capped by a few copies of proteins that are important for infection of the host bacteria, and also for assembly of nascent phage particles. (wikipedia.org)
Despite phage replication in these matrices, lysis of the bacteria was slower than phage propagation, and no reduction of the bacterial population was observed. (bvsalud.org)
Replication1
During fd phage assembly, the phage DNA is first packaged into a linear intracellular nucleoprotein complex with many copies of the phage gene 5 replication/assembly protein. (wikipedia.org)
Bacterial2
The gene 5 protein is then displaced by the gene 8 coat protein as the nascent phage is extruded across the bacterial plasma membrane without killing the bacterial host. (wikipedia.org)
How bacterial cyclases recognize phage infection is not known. (bvsalud.org)
Circular2
This topology was assumed to extend to all other filamentous phages, but it is not the case for phage Pf4, for which the DNA in the phage is single-stranded but topologically linear, not circular. (wikipedia.org)
The genome of strain GDCZ comprised of 1,230,507 bp with an average G + C content of 36.4%, along with a circular CLasMV1 phage: CLasMV1_GDCZ (8,869 bp). (bvsalud.org)
Filamentous2
They are named for their filamentous shape, a worm-like chain (long, thin and flexible, reminiscent of a length of cooked spaghetti), about 6 nm in diameter and about 1000-2000 nm long. (wikipedia.org)
The simplicity of filamentous phages makes them an appealing model organism for research in molecular biology, and they have also shown promise as tools in nanotechnology and immunology. (wikipedia.org)
Genetic1
With the burst of publications on phage isolation and genetic studies during the last decade, several different bioinformatics programs have been used. (bvsalud.org)
Complex1
As simple as it sounds, 'DNA uptake' is mechanistically as complex as conjugation or phage transduction. (asmblog.org)
Production2
Cyclic oligonucleotide-based antiphage signalling systems (CBASS) protect prokaryotes from viral (phage) attack through the production of cyclic oligonucleotides, which activate effector proteins that trigger the death of the infected host1,2. (bvsalud.org)
E. coli ATCC 25922 mutant of RecA, a key component of SOS system, exhibited decreased phage production. (bvsalud.org)
Family1
The morphology of Φ st1 showed strong resemblance to members of the Siphoviridae family. (afpm.org.my)
Study1
In this in vitro and animal study, we demonstrated that the activity of anti-staphylococcal phages is severely impaired in 0.5% plasma or synovial fluid. (bvsalud.org)
Plasma2
The gene 8 protein is inserted into the plasma membrane as an early step in phage assembly. (wikipedia.org)
The inhibition of the phages associated with a reduction in phage adsorption, quantified to 99% at 10% plasma. (bvsalud.org)
Studies1
The fiber diffraction studies identified two structural classes of phage, differing in the details of the arrangement of the gene 8 protein. (wikipedia.org)
Bacteriophages4
Filamentous bacteriophages are among the simplest living organisms known, with far fewer genes than the classical tailed bacteriophages studied by the phage group in the mid-20th century. (wikipedia.org)
A family of bacteriophages containing one genus (Cystovirus) with one member (BACTERIOPHAGE PHI 6). (lookformedical.com)
Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). (lookformedical.com)
The application of lytic bacteriophages (phages) that show specificity towards malodour-producing species could therefore be selectively used to remove malodourous bacteria. (lpmhealthcare.com)
Staphylococcus1
This project is focused on the isolation and characterisation of bacterial species colonising human skin and phages targeting odor-producing Staphylococcus genera. (lpmhealthcare.com)
20232
Thank you for considering to present your work as a poster at Phages 2023. (lpmhealthcare.com)
The single-stranded Ff phage DNA runs down the central core of the phage, and is protected by a cylindrical protein coat built from thousands of identical α-helical major coat protein subunits coded by phage gene 8. (wikipedia.org)
The DNA isolated from fd phage (of genus Inovirus) is single-stranded, and topologically a circle. (wikipedia.org)
This topology was assumed to extend to all other filamentous phages, but it is not the case for phage Pf4, for which the DNA in the phage is single-stranded but topologically linear, not circular. (wikipedia.org)
Lytic1
The highly specific nature of lytic phages can be employed to eliminate/control malodour-producing microbes whilst leaving the remaining axilla-associated microbiota intact. (lpmhealthcare.com)
Proteins2
This distinctive shape reflects their method of replication: the coat of the virion comprises five types of viral protein, which are located in the inner membrane of the host bacterium during phage assembly, and these proteins are added to the nascent virion's DNA as it is extruded through the membrane. (wikipedia.org)
The structures of the phage capsid and of some other phage proteins are available from the Protein Data Bank. (wikipedia.org)
Particle1
That is, the DNA single strand extends from one end of the phage particle to the other and then back again to close the circle, although the two strands are not base-paired. (wikipedia.org)
Structural1
The fiber diffraction studies identified two structural classes of phage, differing in the details of the arrangement of the gene 8 protein. (wikipedia.org)
Genus1
The p1 protein of Ff phage (i. e. genus Inovirus), which is required for phage assembly at the membrane, has a membrane-spanning hydrophobic domain with the N-terminal portion in the cytoplasm and the C-terminal portion in the periplasm (the reverse of the orientation of the gene 8 coat protein). (wikipedia.org)
Structures1
This protein also binds with high affinity to G-quadruplex structures (although they are not present in the phage DNA) and to similar hairpin structures in phage DNA. (wikipedia.org)
Gene3
The gene 8 protein is inserted into the plasma membrane as an early step in phage assembly. (wikipedia.org)
Some strains of phage have a "leader sequence" on the gene 8 protein to promote membrane insertion, but others do not seem to need the leader sequence. (wikipedia.org)
During fd phage assembly, the phage DNA is first packaged into a linear intracellular nucleoprotein complex with many copies of the phage gene 5 replication/assembly protein. (wikipedia.org)
Treatment1
[6] With proper antibiotic treatment, the prognosis for victims is much better than before antibiotics were developed. (mdwiki.org)
Virus1
Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium. (lookformedical.com)