Pseudomonas development biofilms. Medical search. Frequent questions

The present authors hypothesised that bronchoscopy with protected specimen brush may sample biofilm-forming bacteria adherent to the airway wall, whereas traditional sputum collection may not. (ersjournals.com)
Retrieved bacteria were genotyped, and grown in planktonic cultures and as biofilms, and susceptibilities to individual antibiotics and to antibiotic combinations were determined. (ersjournals.com)
Susceptibility to single antibiotics and to antibiotic combinations were not different between planktonically or biofilm-grown bacteria derived from sputum, as compared to those obtained by bronchoalveolar lavage and protected brush. (ersjournals.com)
One problem with relying on sputum samples to guide therapy is that biofilm bacteria may not be adequately sampled via sputum, since sputum expectoration only samples P. aeruginosa that have detached and dispersed from the airway biofilm 5 , 6 . (ersjournals.com)
However, studies have not previously been done to determine whether bacteria retrieved from sputum are more susceptible to antibiotics compared to bacteria retrieved directly from the airway biofilm. (ersjournals.com)
Thus, it is possible that PSB may directly recover P. aeruginosa bacteria that have aggregated as biofilms and adhered to the surface of the airway. (ersjournals.com)
Kolter's research group was influential in the study of bacterial transport systems known as ABC exporters, published some of the earliest examples of experimental evolution through investigations of the stationary phase of bacterial growth, and was foundational in genetic studies of bacteria adhered to surfaces (living within communities called biofilms). (wikipedia.org)
The lab popularized the concept of bacterial biofilm formation as developmental or multicellular microbial processes, and pioneered genetic studies of cellular differentiation, signaling, and division of labor in bacteria. (wikipedia.org)
[13] The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
[8] [5] The first colonist bacteria of a biofilm may adhere to the surface initially by the weak van der Waals forces and hydrophobic effects. (wikipedia.org)
5.1 Vegetative biofilm bacteria are phenotypically different from suspended planktonic cells of the same genotype. (astm.org)
Those unique tissues allow the accumulation of extracellular products and the formation of biofilms that serve as culture media for bacteria and contribute to the development of antibiotic resistance ( 1 ). (cdc.gov)
We have recently reported an increased rate of gram-negative bacteria in the nasopharynx of these patients, including a high proportion of Pseudomonas aeruginosa ( 2 ). (cdc.gov)
Bacteria often switch from a free-living lifestyle to a surface adapted, structured lifestyle known as a biofilm. (southampton.ac.uk)
We found that subpopulations of biofilm bacteria undergo cell death and lysis as a feature of the Pseudomonas aeruginosa biofilm life cycle. (southampton.ac.uk)
We isolated and named this phage, showed that it becomes superinfective to its host during biofilm development, and found that it plays a role in phenotypic diversification of biofilm bacteria. (southampton.ac.uk)
Using confocal laser scanning microscopy, we demonstrated that the drug damaged bacteria within an established biofilm showing that penetration did not limit the activity of this antimicrobial agent against a biofilm. (montana.edu)
When biofilms were grown during exposure to shear forces and to a continuous medium flow allowing the development of robust structures with a complex architecture, CSA-13 reached the bacteria entrapped in the biofilm within 30 min. (montana.edu)
Quorum sensing plays a crucial role in the virulence nature of P. aeruginosa as it helps the bacteria to produce virulence factors and biofilm formation. (ijpsr.com)
In the BacData project, the researchers are investigating two clinically highly relevant biofilm associated infections: the lung infection in cystic fibrosis patients caused by Pseudomonas bacteria and the implant infection as an example of frequently occurring multi-species biofilm infections. (l3s.de)
This power low behavior may benefit bacteria social organization during biofilm formation. (wisc.edu)
Pseudomonas aeruginosa is the gram negative rod shaped bacteria is found in all natural and man made environments. (ukessays.com)
Pseudomonas aeruginosa is a member of gamma proteobacteria class of bacteria. (ukessays.com)
Lysins are a new class of DLAs which are recombinantly produced antimicrobial proteins with a novel mechanism of action associated with the rapid killing of target bacteria, eradication of biofilms and synergy with conventional antibiotics. (kfor.com)
Biofilms are a mixture of complex communities of organisms mostly composed of diverse bacteria that vary depending on the surrounding environmental conditions induced by physical and chemical factors. (intechopen.com)
Observation of biofilms dates back to the seventeenth century when Antonie van Leeuwenhoek observed bacteria from the plaque biofilm of his teeth under his primitive microscope [ 2 ]. (intechopen.com)
1,2 The unique structure of biofilms protects bacteria from the surrounding environment, conferring a capacity for persistence against phagocytosis, oxidative stresses, nutrient/oxygen restriction, metabolic waste accumulation, interspecies competition, and conventional antimicrobial agents. (rsc.org)
2,9-11 A comprehensive understanding of the interactions between bacteria and materials with different surface topographies may pave the way for more effective strategies to control biofilm growth. (rsc.org)
Many antimicrobial agents that are effective against planktonic cells turn out to be ineffective against the same bacteria growing in a biofilm state 9,10 . (bvsalud.org)
Because of the increasing incidence of drug-resistant bacteria, the development of natural antibacterial sources such as medical herbs for the treatment of infectious diseases is necessary. (ac.ir)
Consequently, bacteria such as Streptococcus pneumoniae, Streptococcus pyogenes, Staphylococci, and S. aureus, and some members of the Pseudomonas and Enterobacteriaceae families, are now resistant to nearly all the conventional antibiotics [3]. (nanochemres.org)
Bacteria survive in various environments by forming biofilms. (biomedcentral.com)
In this study, we evaluated the ability of two types of biosurfactants (rhamnolipids and surfactin) to inhibit growth and biofilm formation ability of oral pathogenic bacteria such as Aggregatibacter actinomycetemcomitans , Streptococcus mutans , and Streptococcus sanguinis . (biomedcentral.com)
Rhamnolipids inhibited the growth and biofilm formation ability of all examined oral bacteria. (biomedcentral.com)
Streptococcus mutans , a representative bacteria of tooth decay [ 10 ], forms plaques (biofilm) on tooth surfaces and produces lactate from sugars such as sucrose, which demineralizes teeth and causes dental caries. (biomedcentral.com)
Therefore, removal of biofilms formed by periodontopathogenic bacteria prevents a variety of diseases. (biomedcentral.com)
Anti-bacterial agents and disinfectants are effective against planktonic bacteria (bacteria suspended in liquid), however, they are not effective against biofilm-forming bacteria due to the difficulty of chemicals penetrating the biofilm. (biomedcentral.com)
Therefore, biofilm matrix gives the additional resistance power to bacteria which makes them to not only tolerate harsh conditions but also resistant to antibiotics which lead to the emergence of bad bugs infections like multi drug resistant, extensively drug resistant and totally drug resistant bacteria. (biomedcentral.com)
The presence of potentially pathogenic bacteria and an indwelling catheter predisposes to the development of a nosocomial UTI. (medscape.com)
In addition, bacteria form a biofilm on and within the endotracheal tube that protects them from antibiotics and host defenses. (msdmanuals.com)

Effectiveness of honey on Staphylococcus aureus and Pseudomonas aeruginosa biofilms. (microbiologyresearch.org)
The Company will be presenting two posters describing the initial development of the exebacase MIC method for testing Staphylococci other than Staphylococcus aureus (SoSA) and method verification studies performed to evaluate test performance. (kfor.com)

Understanding the biological characteristics of biofilms, the cause of most known bacterial infections, is the first step to fight against this silent pandemic and to find effective treatments. (ibecbarcelona.eu)
Bacterial infections are a public health problem affecting millions of people worldwid e, and about 80% of them are associated with biofilms , communities formed by different microorganism s' species which co-colonize human tissues and medical devices. (ibecbarcelona.eu)
Only with the continuous development and improvement of efficient antibiofilm strategies we can tackle the recurrence and chronicity caused by P. aeruginosa biofilm infections. (ibecbarcelona.eu)
It is also a relevant pathogen in chronic wounds infections and skin injuries , and in keratitis, a common corneal infection related with bacterial biofilm formation over contact lenses. (ibecbarcelona.eu)
Bacterial biofilms are formed by different species that colonize and grow together on human tissues, causing infections in the urinary tract, lung and kidney among others, and on surfaces of medical devices, as joint prostheses , heart valves and pacemakers, dental and breast implants, sutures and catheters. (ibecbarcelona.eu)
Bacterial biofilms: a common cause of persistent infections. (microbiologyresearch.org)
The formation of a Pseudomonas aeruginosa biofilm, a complex structure enclosing bacterial cells in an extracellular polymeric matrix, is responsible for persistent infections in cystic fibrosis patients leading to a high rate of morbidity and mortality. (montana.edu)
This study confirms the potential of CSA-13 as a new strategy to combat persistent infections involving biofilms formed by P. aeruginosa. (montana.edu)
Therapeutic failures of antibiotics used to treat macrolide-susceptible Streptococcus pyogenes infections may be due to biofilm formation. (microbiologyresearch.org)
Biofilm-associated infections are therefore one of the greatest challenges facing medicine . (l3s.de)
BacData is intended to improve the prognosis of patients suffering from biofilm-associated infections in the long term and, in addition, to create the basis for a personalised therapy of further infectious diseases. (l3s.de)
Furthermore, the ability of the pathogen to build biofilms and to persist e.g. in the lungs of cystic fibrosis (CF) patients facilitates the establishment of chronic infections, which are largely recalcitrant to antimicrobial therapies. (elifesciences.org)
ContraFect is a biotechnology company focused on the discovery and development of DLAs, including lysins and amurin peptides, as new medical modalities for the treatment of life-threatening, antibiotic-resistant infections. (kfor.com)
We believe that the properties of our lysins and amurin peptides will make them suitable for targeting antibiotic-resistant organisms, such as MRSA, Pseudomonas aeruginosa and Acinetobacter baumannii , which can cause serious infections such as bacteremia and pneumonia. (kfor.com)
3 Bacterial biofilms can cause persistent human infections and can foul the surface of medical devices. (rsc.org)
4,5 For example, Pseudomonas aeruginosa , an organism well-known for its capacity to form biofilms, is an opportunistic pathogen and is one of the top three causes of opportunistic human infections, 6 causing nosocomial infections in catheter lines, or chronically infecting the lungs of cystic fibrosis patients. (rsc.org)
3,6 Biofilm formation by P. aeruginosa is responsible for antimicrobial tolerance and causes major problems for treatment of infections. (rsc.org)
3,8 Therefore, it is important to develop biomaterials that can control biofilm growth thereby reduce infections. (rsc.org)
Pseudomonas aeruginosa is the prevalent and opportunistic pathogen which causes infections that are difficult to treat. (preventit.in)
Results: Eighty percent of the isolated samples (16/20) could form a biofilm, and most of these were isolated from wound infections. (ac.ir)
Pseudomonas aeruginosa ( P. aeruginosa ), Acinetobacter species, Klebsiella species, Escherichia coli ( E. coli ) and Enterobacter cloacae are the most commonly implicated Gram-negative pathogens in HABP/VABP infections. (klfy.com)
Pseudomonas aeruginosa is a significant cause of nosocomial infections. (nanochemres.org)

In this study, we present a novel transcriptional regulator, PA1226, which modulates biofilm formation and virulence in P. aeruginosa. (diagenode.com)

The virulence factors of Candida albicans are germ tube and biofilm formation, adherence to host tissues, and production of hydrolytic enzymes. (hindawi.com)
The in-vitro analysis showed a marked decrease in the production of virulence factors such as pyocyanin, rhamnolipids, protease, elastase, exopolysaccharides, HCN gas production and biofilm formation. (ijpsr.com)
These findings indicate a new anti-infective and alternative therapeutic approaches to combat the virulence factors of Pseudomonas aeruginosa and explore further the potential of fungal secondary metabolites from other saprophytic fungi colonizing decaying substrata of medicinal plants. (ijpsr.com)
The las system regulates the production of virulence factors such as LasA protease, LasB elastase, alkaline protease, exotoxin A and biofilm formation. (ijpsr.com)

The problematic opportunistic pathogen Pseudomonas aeruginosa is a major cause of infection in patients with underlying or immunocompromising conditions. (elifesciences.org)

Anisotropy also plays a large role in the formation of bacterial communities called biofilms. (wisc.edu)

Biofilms are notorious for their resistance to environmental stresses, including antimicrobial compounds. (southampton.ac.uk)
Treatment of biofilms with nitric oxide also reduced their resistance to antimicrobial compounds. (southampton.ac.uk)
Dr. Ambler's presentation will review the current standards for antimicrobial susceptibility testing (AST) for the determination of antibacterial activity of therapeutic agents and the implications for clinical development. (kfor.com)
Planktonic and biofilm cells also exhibit different susceptibilities to a certain antimicrobial concentration. (bvsalud.org)
The antimicrobial properties of Melaleuca essential oil alternifolia to oral biofilms have been reported in several studies [9-12]. (bvsalud.org)

Therefore, inhibiting the growth of oral pathogens, as well as effectively preventing biofilm formation contribute to reducing these risks. (biomedcentral.com)

In conclusion, sputum collection provides as much information as bronchoscopy for characterising the genotype and antibiotic susceptibility of chronic Pseudomonas aeruginosa infection in patients with stable cystic fibrosis. (ersjournals.com)
This tolerance often links biofilms with persistent and chronic infection, and provides ideal conditions for the acquisition or evolution of AMR. (southampton.ac.uk)
Disrupting biofilm formation has important implications in public health in reducing infection rates. (columbia.edu)
GAS) is always of clinical significance in wounds where it can initiate infection, destroy skin grafts and persist as a biofilm. (microbiologyresearch.org)

Ammeri RW, Hassen W, Mehri I, khelifi N, Hassen A. Pentachlorophenol Effect on Auxin Production by Pseudomonas fluorescens GU059580 and Its Application in Wastewater Bioremediation. (lidsen.com)
We determined the effect of the role of PCP surfactant for the biofilm and auxin production of the selected bacterial strain of P. fluorescens GU059580. (lidsen.com)
Subsequent work showed similar behaviour as P. aeruginosa aligned within subcellular-nanogratings, 13 Escherichia coli orientated towards surface line patterns, 14 and Pseudomonas fluorescens were trapped preferentially in surface trenches. (rsc.org)

A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
Biofilms are complex, highly dynamic, structured ecosystems formed by a community of different microorganisms living attached to inert or living surfaces and embedded in a matrix designated extracellular polymeric substance (EPS). (intechopen.com)
Several other microorganisms come after entering in this very competitive ecosystem where quorum sensing and antibiotic resistance are determinants in the development of the community. (intechopen.com)
Several studies focusing on the efficacy of mouthwashes with diverse chemical composition demonstrated that combination of sodium fluoride and sodium lauryl sulfate as well as essential oils is able to diminish the metabolic activity of microorganisms present in the dental biofilm 11-13 . (bvsalud.org)
Biofilms are three-dimensional structures consisting of various microbial populations attached to a surface by extracellular polymeric substances (EPS) produced by these microorganisms [ 1 ]. (biomedcentral.com)
The development of hospital-acquired pneumonia (HAP) represents an imbalance between normal host defenses and the ability of microorganisms to colonize and then invade the lower respiratory tract. (medscape.com)

In this study, the effect of manuka honey on S. pyogenes (M28) was investigated in vitro with planktonic and biofilm cultures using MIC, MBC, microscopy and aggregation efficiency. (microbiologyresearch.org)
The genes responsible for the biofilm formation are quantified by comparing the planktonic and biofilm samples. (ukessays.com)
The results from the planktonic and biofilm gene expression results are compared. (ukessays.com)

Pseudomonas aeruginosa is one of the most emerging multidrug-resistant opportunistic pathogens, which poses a threat to immunocompromised patients. (ijpsr.com)
Because aerobic gram-negative bacilli (eg, Pseudomonas aeruginosa) are the major pathogens associated with HAP, the pathophysiology of nosocomial pneumonia relates to the destructive effect on lung tissue. (medscape.com)

Mutation in the gene encoding this regulator abolished the ability of P. aeruginosa to produce biofilms in vitro, without any effect on the planktonic growth. (diagenode.com)
Mu DNA transposition complexes were assembled with MuA transposase and an artificial mini-Mu transposon in vitro , and introduced into Pseudomonas aeruginosa by electroporation. (microbiologyresearch.org)

Biofilm growth reactors are engineered to produce biofilms with specific characteristics. (astm.org)

In addition to the pure science implications of these studies, a potential application of this would be to integrate such chips into medical devices that are common sites of biofilm formation, such as catheters, and then use the chips to limit bacterial colonization. (columbia.edu)
Proteus and Pseudomonas species are the organisms most commonly associated with biofilm growth on catheters. (medscape.com)

Although physical methods are fundamental for removing oral biofilms, there is a wide variation in its efficiency due to individual differences. (biomedcentral.com)

The control of the dental biofilm allows the maintenance of oral health and prevents the development of diseases such as caries and periodontal disease, which are strongly related to tooth loss [1]. (bvsalud.org)

The goal in biofilm research and efficacy testing is to choose the growth reactor that generates the most relevant biofilm for the particular study. (astm.org)

Mu transposition complexes were used for transposon mutagenesis of Pseudomonas aeruginosa strain PA68. (microbiologyresearch.org)
Pseudomonas aeruginosa strain ATCC 27853 was purchased from Pasteur Institute, Tehran, Iran. (nanochemres.org)

In addition, biofilms were grown in microscope coverslips treated as described above, followed by staining with Propidium Iodide and Fluoresceine for visualization with a confocal laser scanning microscopy. (bvsalud.org)
The confocal laser scanning microscopy analysis demonstrated that all mouthwashes, except for cetylpyridinium chloride, were able to cause membrane damage after 60 s of incubation with S. gordonii biofilms. (bvsalud.org)

We showed that strains of Pseudomonas aeruginosa with increased mutation rates (caused by deletion of genes involved in DNA mismatch repair) produced more biofilm biomass in laboratory experiments. (southampton.ac.uk)
Bioaugmentation by Pseudomonas strains is widely used for the removal of pollutants in wastewater. (lidsen.com)
Strains such as Pseudomonas aeruginosa produce biosurfactants, including rhamnolipids [ 23 ]. (biomedcentral.com)

Along with the intrinsic antibiotic resistance property of P. aeruginosa, it is also know to grow and from cell aggregates or biofilm in the extracellular matrix. (preventit.in)

Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
Biofilms can be found in a variety of habitats, on free‐living, on the surface of other organisms or inert surfaces, both in aquatic as well as terrestrial environments. (intechopen.com)
The aim of this study was to evaluate the bactericidal effect of calcium oxide (CaO) against Pseudomonas aeruginosa biofilms on quail eggshells and major egg contacting surfaces (stainless steel, plastic, and rubber). (elsevierpure.com)
All the CaO treatments significantly reduced P. aeruginosa biofilms on all tested surfaces as compared to controls. (elsevierpure.com)
Here, we investigated bacterial attachment, cell alignment and biofilm formation of Pseudomonas aeruginosa on periodic nano-pillar surfaces with different pillar spacing. (rsc.org)
Bacterial cells can colonize surfaces and form biofilms that consist of microbial cells embedded in extracellular polymeric substances (EPS). (rsc.org)

The purpose of this study was to reconfirm the high incidence of P. aeruginosa isolations from the oropharynx of NGT-fed elderly patients, determine its antibiotic susceptibility, and explore the possibility of biofilm formation on the feeding tube. (cdc.gov)

Subpopulations of cells within the biofilm differentiate to perform various activities for motility, matrix production, and sporulation, supporting the overall success of the biofilm. (wikipedia.org)
A function of Pseudomonas aeruginosa PAO pili: twitching motility. (microbiologyresearch.org)
The pilG gene product, required for Pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric, single-domain response regulator CheY. (microbiologyresearch.org)

[2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)
A cohesive structure of a multi‐layer of extracellular polymeric substances (EPS) such as polysaccharides and proteins is the base of biofilm structural organization. (intechopen.com)

Fibrinogen induces biofilm formation by Streptococcus suis and enhances its antibiotic resistance. (microbiologyresearch.org)
Aim: The aim of this study was to investigate the metabolic activity of Streptococcus mutans biofilms after treatment with mouthwashes with different compositions. (bvsalud.org)
14 (2004) studied the effects of mouthwashes containing essential oils, triclosan, cetylpyridinium chloride and chlorhexidine against Streptococcus gordonii biofilms. (bvsalud.org)
Streptococcus sanguinis is another plaque-forming strain that has been reported to cause infective endocarditis by forming biofilms on the endocardium and heart valves [ 11 ]. (biomedcentral.com)

Biofilms are increasingly recognised as the predominant mode of bacterial growth including within medical, engineered, and environmental contexts. (southampton.ac.uk)
Rhamnolipids inhibit oral bacterial growth and biofilm formation by A. actinomycetemcomitans Y4, and may serve as novel oral drug against localized invasive periodontitis. (biomedcentral.com)
Thus, there is a requirement for the development of a simpler and more effective oral care capable of inhibiting oral bacterial growth and biofilm formation. (biomedcentral.com)

The established biofilm is transferred to a new receiver plate for disinfectant efficacy testing. (astm.org)

[10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)
An interactive web-based Pseudomonas aeruginosa genome database: discovery of new genes, pathways and structures. (microbiologyresearch.org)
The exopolysaccharide genes such as pilB, pslA, lecA, ureB and alginate genes are responsible for the formation of pseudomonas biofilms. (ukessays.com)
The locus of the psl polysacchide genes encode the cells to adhere to a surface and thus maintaining the biofilm structure. (ukessays.com)
Metabolic activity of the bacterial biofilm communities have altered as compared to the planktonic one such as increased rates of EPS production, activation or inhibition of particular genes associated with biofilm formation and decreased growth rate [ 39 ]. (biomedcentral.com)

Bactericidal effects were found in both planktonic cultures and biofilms, although higher concentrations of manuka honey were needed to inhibit biofilms. (microbiologyresearch.org)
In particular, surface modifications that physically create rational surface topographies have attracted attention in recent years, and have shown to inhibit bacterial attachment and biofilm growth without the use of antimicrobials. (rsc.org)
Techniques to inhibit biofilm formation are essential and have wide applications. (biomedcentral.com)
The results clearly demonstrated that rhamnolipids inhibit biofilm formation after the initiation process, however, they do not affect attachment or maturation. (biomedcentral.com)

Paul was appointed as the Research Leader of Microbiology in August 2002 and manages the Microbiology Research and Development Group. (edu.au)

P. aeruginosa, a well-known biofilm-producing microorganism ( 4 , 5 ), may be exploiting the NGT to create a thriving habitat. (cdc.gov)

In the current study, we investigated the characteristics of bacterial strain Pseudomonas sp. (mdpi.com)
This bacterial strain showed plant growth-promoting properties, including biofilm formation and siderophore activity. (mdpi.com)
Bacterial metabolism selected lignin model compounds (vanillin, vanillate, 4- hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a Introduction culture collection. (lu.se)

My research group aims to understand how biofilms develop and disperse and how they respond to environmental stresses including therapeutic compounds. (southampton.ac.uk)
The development of colony biofilms by Pseudomonas aeruginosa is affected by redox-active compounds called phenazines. (columbia.edu)

Biofilms were grown on coverslips in the presence of plant extracts and processed for scanning electron microscopy (SEM). (hindawi.com)
Biofilm reduction after CaO treatment was also observed by field emission scanning electron microscopy (FE-SEM). (elsevierpure.com)
To corroborate these results, biofilms were observed by scanning electron microscopy (SEM) and confocal microscopy. (biomedcentral.com)

Glycopeptidolipids are indispensable for initial surface attachment during biofilm formation in Mycobacterium smegmatis [ 111 ]. (biomedcentral.com)
Growth pattern of Mycobacterium species are different such as M. chelonae and M. fortuitum form biofilm as vertical and entire surface growth, respectively [ 93 ]. (biomedcentral.com)

The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)
Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)
1.4 This test method describes how to sample the biofilm and quantify viable cells. (astm.org)
Manuka honey permeated 24 h established biofilms of S. pyogenes, resulting in significant cell death and dissociation of cells from the biofilm. (microbiologyresearch.org)
The functioning of the EPS matrix in holding cells of Pseudomonas aeruginosa together and forming the mature biofilms. (ukessays.com)
The planktonic cells grow very rapidly whereas the bacterial adhering takes some time to form biofilm in other case. (ukessays.com)

By using condensed matter techniques, we present for the first time the dynamics of colony formation at early stages of biofilm development for Pseudomonas aeruginosa. (wisc.edu)
iii) colony formation and biofilm maturation. (biomedcentral.com)

The PCP removal from wastewater showed an improvement of about 96.5% after the addition of Tween 80, whereas significant biofilm formation was found in the mineral liquid medium supplemented with PCP and Tween 80, with a value of 3.78. (lidsen.com)

In this review, researchers decipher the implication of P . aeruginosa in biofilms and pave the way to find solutions in a near future. (ibecbarcelona.eu)

20 generations, a few days of biofilm growth) within biofilms. (southampton.ac.uk)
Flavopiridol also exhibited antifungal activity by restricting fungal growth and limiting fungal biofilm formation and conidial adhesion. (researchsquare.com)
Surface topography designed to achieve spatial segregation has shown promise in delaying bacterial attachment and biofilm growth. (rsc.org)
Methods: S. mutans biofilms were growth on polystyrene plates during 18 h, washed with sterile saline and treated with the following mouthwashes during 1 min: Listerine®, Oral B®, Parodontax® and Periogard® with and without alcohol. (bvsalud.org)

5.2 The purpose of this test method is to direct a user in how to grow, treat, sample and analyze a Pseudomonas aeruginosa biofilm using the MBEC Assay. (astm.org)
1.1 This test method specifies the operational parameters required to grow and treat a Pseudomonas aeruginosa biofilm in a high throughput screening assay known as the MBEC (trademarked) 2 (Minimum Biofilm Eradication Concentration) Physiology and Genetics Assay. (astm.org)
The observations were largely in concordance with the biofilm assay results. (biomedcentral.com)

Biofilm production was determined using 96-well polystyrene plates, and auxin production was determined using spectrophotometric measurement at 535 nm. (lidsen.com)

Review and approval of new AST methods by standards development organizations, such as the Clinical and Laboratory Standards Institute (CLSI), is essential. (kfor.com)
In this position he was responsible for the development of rapid methods for the isolation and identification of Cryptosporidium and Giardia from water. (edu.au)

The development of the biofilm starts with the attachment of the freely moving planktonic cell to a surface. (ukessays.com)
Biofilm formation occurs as a result of a sequence of events: microbial surface attachment, cell proliferation, matrix production and detachment 8 . (bvsalud.org)

The unique ability of the organism is forming biofilm. (ukessays.com)
The biofilms are formed by the organism with the association with a surface. (ukessays.com)

Conclusions: The treatment of biofilms with mouthwashes containing 0.2% chlorhexidine or essential oils induced significant reduction in S. mutans metabolism. (bvsalud.org)
One important characteristic of S. mutans in promoting caries development is the ability to adhere firmly to the tooth surface in the presence of sucrose. (bvsalud.org)

The misuse or abuse of antimicrobials in the human, veterinary and agriculture sectors is the driving cause for worldwide AMR development. (preventit.in)

[8] Biofilms can form on the teeth of most animals as dental plaque , where they may cause tooth decay and gum disease . (wikipedia.org)
A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)
This is an exciting new application for CMOS technology that will provide new insights into how biofilms form," says Shepard. (columbia.edu)
Such a biofilm can also form on organs and implants, such as knee and hip prostheses or dental and cochlear implants. (l3s.de)
We found that Pseudomonas aeruginosa does not follow an isotropic random walk as commonly assumed, but instead obeys a new form of polysaccharide-guided dynamics such that the distribution of surface visitation follows a power law. (wisc.edu)
The microcarriers made up of dextrons in the range of 60-90µm in size are used as a surface for Pseudomonas aeruginosa to form biofilms. (ukessays.com)
Aggregatibacter actinomycetemcomitans has been strongly associated with localized aggressive periodontitis via its ability to form biofilms in the subgingival cavity [ 12 ]. (biomedcentral.com)

Pseudomonas aeruginosa is one of the examples among drug-resistant opportunistic microbes that is capable of infecting patients suffering from cystic fibrosis, chronic obstructive pulmonary diseases, HIV, and immunocompromised patients. (ijpsr.com)

Anatomy6

Organisms40

Diseases11

Chemicals and Drugs107

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes51

Disciplines and Occupations4

Technology, Industry, Agriculture8

Information Science4

Health Care14

Bacteria41

Staphylococcus2

Infections22

Formation and virulence1

Virulence factors4

Opportunistic pathogen1

Communities called biofilms1

Antimicrobial5

Preventing biofilm formation1

Infection4

Fluorescens3

Microorganisms8

Planktonic and biofilm3

Pathogens2

Vitro2

Produce biofilms1

Catheters2

Oral biofilms1

Dental biofilm1

Relevant biofilm1

Aeruginosa strain2

Confocal laser scan2

Strains3

Extracellular matrix1

Surfaces6

Susceptibility1

Motility3

Proteins2

Streptococcus4

Bacterial growth3

Efficacy1

Genes5

Inhibit4

Microbiology1

Microorganism1

Strain3

Compounds2

Microscopy3

Mycobacterium2

Cells6

Colony formation2

Wastewater1

Pave the way1

Growth4

Assay3

Polystyrene plates1

Methods2

Attachment2

Organism2

Mutans2

Antimicrobials1

Form7

Chronic1