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  • semialdehyde
  • The toxic organic compounds can be broken down into molecules that are metabolizable by all organisms, such as 2-hydroxymuconic semialdehyde (2-HMS) 3 . (igem.org)
  • strain
  • In vivo metabolic analysis showed a significant decrease of the acetyl-CoA pool size in the strain with the highest 3-HP titer, suggesting the supply of acetyl-CoA is a potential bottleneck for further improvement. (biomedcentral.com)
  • xylE
  • In 2010, the Lethbridge iGEM team confirmed that xylE when introduce to E. coli hydrolyzes catechol to 2-HMS demonstrating and that this critical step can successfully be performed to convert toxic chemicals to metabolic intermediates 7 . (igem.org)
  • bacteria
  • Octylphenol polyethoxylates (OPEOn) are surfactants and prone to be degraded into xenoestrogenic metabolites, such as octylphenol and OPEOn (n=1-2), are estrogenic-like compounds, and persistent to be degraded by bacteria in the environments. (ncu.edu.tw)
  • In: Sokatch JR, Ornston LN (eds) The bacteria: the biology of Pseudomonas , vol 10. (springer.com)
  • It is noteworthy that in nature 3-HP serves in central metabolism either as an intermediate of C3/C4 assimilation cycles or as an input carbon source in some bacteria and archaea. (biomedcentral.com)
  • isolates
  • Among the isolates, Pseudomonas nitroreducens TX1 can grow on 0.05% to 20% of OPEOn with a specific growth rate of 0.34-0.44 hr-1. (ncu.edu.tw)
  • metabolism
  • Studies of Porphyrin Metabolism, 3. (unt.edu)
  • 2. Role of oxygen in the metabolic regulation : Besides the well known … More role of oxygen in the energy metabolism, oxygen has been shown to play an important physiological role in metabolic regulation through oxygenase-catalyzed reactions. (nii.ac.jp)
  • Metabolism of 4-amino-3-hydroxybenzoic acid by Bordetella sp. (wikipedia.org)
  • Microorganisms
  • There are two major routes that have been developed for 3-HP synthesis in engineered microorganisms including Escherichia coli , Saccharomyces cerevisiae and Pyrococcus furiosus . (biomedcentral.com)
  • pathways
  • To address the decontamination of (1) toxic organic compounds such as naphthene and catechol found in the tailings ponds, natural pathways that can break down the toxic chemicals can be utilized. (igem.org)
  • However, interestingly, benzoate-grown cells induce the ortho and meta pathways for the biodegradation of catechol, by inducing simultaneously catechol 1,2-dioxygenase and 2,3-dioxygenase, respectively, in the ratio of 7:1. (springer.com)
  • dehydrogenase
  • The enzyme 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate dehydrogenase uses 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate and NAD+ to produce 3-methylcatechol, NADH and CO2. (wikipedia.org)
  • Glyoxalase
  • The principal physiological function of glyoxalase I is the detoxification of methylglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. (wikipedia.org)
  • cleave
  • 1,2-CTD uses Fe3+ as a cofactor to cleave the carbon-carbon bond between the phenolic hydroxyl groups of catechol, thus yielding muconic acid as its product. (wikipedia.org)
  • In contrast, 2,3-CTD utilizes Fe2+ as a cofactor to cleave the carbon-carbon bond adjacent to the phenolic hydroxyl groups of catechol, thus yielding 2-hydroxymuconaldehye as its product. (wikipedia.org)
  • acid
  • The spatial distribution of 2,4-dichlorophenoxyacetic acid (2,4-D) degrader microhabitats as a function of 2,4-D degrader abundance is investigated by using a soil microsampling strategy combined with a mathematical spatial analysis. (readabstracts.com)
  • 3-Hydroxypropionic acid (3-HP) is an important platform chemical, serving as a precursor for a wide range of industrial applications such as the production of acrylic acid and 1,3-propanediol. (biomedcentral.com)
  • Molecular
  • We have employed a suite of molecular, bioinformatics, and biochemical tools to interrogate the thermodynamically limiting steps of H{sub 2} production from fatty acids in syntrophic communities. (unt.edu)
  • Accumulation
  • Metabolomics analysis showed the accumulation of intracellular 3-hydroxypropionyl-CoA at stationary phase with the addition of 3-HP into the cultured medium, indicating 3-HP was first converted to its CoA derivatives. (biomedcentral.com)
  • gene
  • Examples of recent gene duplication events in signaling as well as dioxygenase clusters are present, indicating selective gene family expansion as a relatively recent event in D. aromatica 's evolutionary history. (biomedcentral.com)
  • The predictions of the final version of the model, which included three rounds of refinements, are consistent with the experimental results for (1) 91% of the wild-type growth phenotypes, (2) 94% of the gene essentiality results, and (3) 94% of the mutant growth phenotypes. (biomedcentral.com)
  • Pathway
  • The enzyme, studied from phytopathogenic bacteria such as Pseudomonas savastanoi , is involved in a pathway for the production of (indol-3-yl)acetate (IAA), the main auxin hormone in plants. (qmul.ac.uk)
  • microorganisms
  • however, some fundamental questions remain, including (1) how much microbial biomass is present in the oceanic crust, (2) where do the microorganisms originate, and (3) what are their metabolic functions. (frontiersin.org)
  • tryptophan
  • Purification and properties of tryptophan 2-monooxygenase. (qmul.ac.uk)
  • 5. Emanuele, J.J. and Fitzpatrick, P.F. Mechanistic studies of the flavoprotein tryptophan 2-monooxygenase. (qmul.ac.uk)
  • Enzymology
  • In: Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1293 (2). (iisc.ernet.in)
  • NADH
  • The enzyme 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate dehydrogenase uses 1,2-dihydroxy-6-methylcyclohexa-3,5-dienecarboxylate and NAD+ to produce 3-methylcatechol, NADH and CO2. (wikipedia.org)
  • Microbiology
  • Metabolic engineering of Escherichia coli for improving L: -3,4-dihydroxyphenylalanine (L: -DOPA) synthesis from glucose Journal of Industrial Microbiology & Biotechnology, 38, 1845-1852. (unam.mx)
  • In: Current Microbiology, 20 (2). (iisc.ernet.in)
  • FEMS Microbiology Ecology, 72 (3), 464−475. (etis.ee)
  • FEMS Microbiology Ecology, 70 (3), 446−455.10.1111/j.1574-6941.2009.00754.x. (etis.ee)
  • iron
  • Dioxygenase Model Studies: Reactionof Oxygen with Iron Catecholates", Lauffer, R. B. (umn.edu)
  • Iron-containing dioxygenases can be subdivided into three classes on the basis of how iron is incorporated into the active site: those employing a mononuclear iron center, those containing a Rieske [2Fe-2S] cluster, and those utilizing a heme prosthetic group. (wikipedia.org)
  • Most mononculear iron dioxygenases are members of the cupin superfamily in which the overall domain structure is described as a six-stranded β-barrel fold (or jelly roll motif). (wikipedia.org)
  • At the center this barrel structure is a metal ion, most commonly ferrous iron, whose coordination environment is frequently provided by residues in two partially conserved structural motifs: G(X)5HXH(X)3-4E(X)6G and G(X)5-7PXG(X)2H(X)3N. (wikipedia.org)
  • Two important groups of mononuclear, non-heme iron dioxygenases are catechol dioxygenases and 2-oxoglutarate (2OG)-dependent dioxygenases. (wikipedia.org)
  • In the 2OG-dependent dioxygenases, ferrous iron (Fe(II)) is also coordinated by a (His)2(Glu/Asp)1 "facial triad" motif. (wikipedia.org)
  • The active site of catechol dioxygenases most frequently contains iron, but manganese-containing forms are also known. (wikipedia.org)
  • structure
  • Structure and Function of Dioxygenases. (umn.edu)
  • Structure of Nitrilotriacetato.3,5-di-tert-butylcatecholato. (umn.edu)
  • Therefore, 1,2-CTD may bind to the cell lipid membrane via its terminal phospholipids and thus have greater access to the phenolic hydrocarbons vital in lipid membrane structure. (wikipedia.org)