With Prof. Münck, Que studied the mechanism of the protocatechuate 3,4-dioxygenase enzyme, using Mössbauer and EPR spectroscopies, as well as inhibition studies. (wikipedia.org)
Pathway3
2021. Separate upper pathway ring cleavage dioxygenases are required for growth of Sphingomonas wittichii strain RW1 on dibenzofuran and dibenzo-p-dioxin. (nih.gov)
The pcaH was significant in the β-ketoadipate pathway [9] where the pathway as it conserves biochemically, and is a major class of non-heme-iron containing dioxygenase [ 10 ]. (omicsonline.org)
Moreover, a dioxygenase gene, nidA3B3, was detected in Mycobacterium vanbaalenii PYR-1 as an alternate degradation pathway which could catalyze both the initial dihydroxylation of pyrene [ 11 ] to be pyrene cis-4,5-dihydrodiol, and an alternate detoxification pyrene pathway to be pyrene cis-1,2-dihydrodiol [ 12 ]. (omicsonline.org)
Enzyme3
Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. (openmicrobiologyjournal.com)
The enzyme contained 2 g-atoms of iron per mol of protein. (shengsci.com)
PAHs could be degraded by bacteria under aerobic conditions through the initial oxidation of the aromatic ring, which is catalyzed by the dioxygenase enzyme. (omicsonline.org)
nidA and nidB genes are encoding genes for large and small subunits of napthalene-inducible dioxygenase [ 7 , 8 ]. (omicsonline.org)
Cleavage1
Dehydrogenation of phenanthrene-cis-9,10-dihydrodiol to produce the corresponding diol, followed by ortho-cleavage of the oxygenated ring, produced 2,2'-diphenic acid. (afpm.org.my)
Degradation1
New metabolic pathways of phenanthrene and a better understanding of the phenoloxidases and dioxygenase mechanism involved in degradation of phenanthrene were explored in this research. (afpm.org.my)
Extracts1
The identification of 2,2'-diphenic acid in culture extracts indicates that phenanthrene was initially attacked through dioxigenation at C9 and C10 to give cis-9,10-dihydrodiol. (afpm.org.my)
Soil1
Draft whole-genome sequence of the antibiotic-producing soil isolate Pseudomonas sp. (paperbase.net)
This nanocomposite was prepared with slight modification of nanocrystalline cellulose (NCC) with cationic surfactant of cetyltriammonium bromide (CTAB) and further decorated with 3-mercaptopropionic acid (3-MPA) capped CdS QDs. (afpm.org.my)
Common1
The 2,3-dihydrobenzofuran derivative, 7-acetyl-4,6-dimethoxy-2-isopropenyl-2,3-dihydrobenzofuran, had an ED 50 value of 1.3 μg (5.4×10 -9 mol)/cm 2 against the common cutworm. (go.jp)
High2
In 2003, Que reported the crystallographic and spectroscopic characterization of a low spin (S = 1) Fe(IV)=O. The first crystallographic example of a high spin (S = 2) Fe(IV)=O was achieved in 2009, and in 2011, Que proved that the ligand used in the previous study could also support a tricationic cyanoiron(IV) complex. (wikipedia.org)
low-molecular-weight (LMW) compounds consist of two or three rings, and high-molecular-weight (HMW) compounds, more than three rings [ 3 ]. (omicsonline.org)
Form1
More specifically, one of Que's focuses is on homoprotocatechuate (HPCA) 2,3-dioxygenase, which mediates the electron transfer between catechol substrates and O2 to form a [M(II)(semiquinone)superoxo] intermediate. (wikipedia.org)
Journal2
Journal of hazardous materials, 177 (1-3). (csircentral.net)
The Pseudomonas putida peptidoglycan-associated outer membrane lipoprotein is involved in maintenance of the integrity of the cell cell envelope. (paperbase.net)
Pseudomonas is a genus of Gram-negative , rod-shaped bacteria. (kenyon.edu)
5 Pseudomonas contains a number of scientifically and medically studied bacteria, such as the opportunistic pathogen P. aeruginosa , and there is increasing availability of Pseudomonas strain genome sequences. (kenyon.edu)
In a metagenomic analysis of the microbial population in petroleum pipelines, Pseudomonas stutzeri was detected as the most abundant hydrocarbon degrading organism. (kenyon.edu)
Researchers were able to amplify the genes for the enzymes used in the degradation of hydrocarbons from Pseudomonas stutzeri . (kenyon.edu)
Consistent with its relative abundance, researchers confirmed with PCR that Pseudomonas was actively expressing alkane hydroxylase genes and was responsible for up to 20% of total gene expression. (kenyon.edu)
Aromatic5
13 Pseudomonas stutzeri's relative abundance was seen as a result of its ability to utilize aromatic hydrocarbons such as xylene, toluene, phenol, as well as polycyclic aromatic hydrocarbons , such as naphthalene . (kenyon.edu)
These enzymes include: benzoate dioxygenase, toluene 1,2-dioxygenase, and catechol 1,2-dioxygenase, which play a role in aromatic hydrocarbon degradation, and methanol dehydrogenase, which is involved in aliphatic hydrocarbon degradation. (kenyon.edu)
As far as catabolic functions, the clc element harbored, in addition to the genes for chlorocatechol degradation, a complete functional operon for 2-aminophenol degradation and genes for a putative aromatic compound transport protein and for a multicomponent aromatic ring dioxygenase similar to anthranilate hydroxylase. (nih.gov)
Advanced oxidation processes (APOs) of aromatic compounds, particularly of phenol, yield several benzene derivatives, such as hydroquinone, catechol, and resorcinol, as intermediate metabolites of its transformation. (hindawi.com)
Results Bacterial strains ZD 4-1 and ZD 4-3 were identified asComamonas testosteroni and Pseudomonas aeruginosa by 16S rDNA sequence analysis, respectively.The growth of the two strains was observed on a variety of aromatic hydrocarbons. (besjournal.com)
Strains4
Objective To investigate the characteristic and biochemical mechanism about the phenolbiodegradation by bacterial strains ZD 4-1 and ZD 4-3. (besjournal.com)
Methods Bacterial strains ZD 4-1 and ZD4-3 were isolated by using phenol as the sole source of carbon and energy, and identified by 16SrDNA sequence analysis. (besjournal.com)
The strains ZD 4-1and ZD 4-3 metabolized phenol via ortho-pathways and meta-pathways, respectively. (besjournal.com)
Conclusion The strains ZD 4-1 and ZD 4-3 metabolize phenol throughortho-pathways and meta-pathway, respectively. (besjournal.com)
Functional1
1996. Catechol 2,3-dioxygenases functional in oxygen-limited (hypoxic) environments. (nih.gov)
Phenol2
The formation of hydroquinone and -benzoquinone at early stages of phenol oxidation increases the toxicity of phenol wastewaters, showing that these compounds were more toxic and less degradable than the original pollutant [ 2 ]. (hindawi.com)
Characterization of Phenol Biodegradation by Comamonas testosteroni ZD4-1 and Pseudomonas aeruginosa ZD4-3[J]. Biomedical and Environmental Sciences, 2003, 16(2): 163-172. (besjournal.com)
Protein1
ErbB-3 binding protein 1 (Ebp1) is a host protein which binds ErbB-3 receptor to induce signalling events for cell growth regulation. (bvsalud.org)
Contamination4
Species in the bacterial genus Pseudomonas present high potential for hydrocarbon degradation due to their metabolic diversity, their abundance in microbial communities, and their resistance to chemical remediation agents present at contamination sites. (kenyon.edu)
Pseudomonas has shown robust response to oil contamination both in vitro and in situ . (kenyon.edu)
A 2013 study found that Pseudomonas was able to respond to new oil contamination by increasing its abundance and changing its community structure. (kenyon.edu)
12 Microbial community differentiation was observed in the Pseudomonas genus in response to oil contamination. (kenyon.edu)
Bacterial2
10 Pseudomonas species have been found to dominate hydrocarbon degrading bacterial communities even in the Artic-an environment that entails extreme temperature conditions, limited availability of nutrients such as nitrogen, and low levels of available water. (kenyon.edu)
In these mainly marine organisms, above a certain population density, light is produced continuously by the action of bacterial luciferase (LuxAB), which oxidizes a long-chain aldehyde such as tetradecanal in the presence of FMNH 2 and oxygen. (nih.gov)
Environments1
The metabolic versatility of the genus allows Pseudomonas species to inhabit a wide variety of environments. (kenyon.edu)
Efficient1
2 There is a great need for efficient and ecologically friendly remediation strategies. (kenyon.edu)
Properties1
The properties to use 3- and 4-chlorocatechol are determined by a self-transferable DNA element, the clc element, which normally resides at two locations in the cell's chromosome. (nih.gov)