Pseudomonas aeruginosa biofilm formation. Medical search. Frequent questions

Pseudomonas aeruginosa is an environmental opportunistic pathogen that is the main cause of morbidity in cystic fibrosis (CF) patients. (aiche.org)
P. aeruginosa , an opportunistic pathogen, establishes a chronic infection in CF with a phenotype of overproduction of an exopolysaccharide (alginate) due to host-directed mutagenesis. (marshall.edu)
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen which is characterized by its ability to form biofilm, on biotic and abiotic surfaces, makes it particularly resistant to host's immune defenses and current antibiotic therapies. (alexjvs.com)
Pseudomonas aeruginosa is a biofilm-forming opportunistic pathogen which causes chronic infections in immunocompromised patients and leads to high mortality rate. (frontiersin.org)
It is a biofilm-forming opportunistic pathogen causing life-threatening chronic infections in immunocompromised individuals with diseases like burn wounds, urinary tract infections, and respiratory infections ( Gellatly and Hancock, 2013 ). (frontiersin.org)
The problematic opportunistic pathogen Pseudomonas aeruginosa is a major cause of infection in patients with underlying or immunocompromising conditions. (elifesciences.org)
4,5 For example, Pseudomonas aeruginosa , an organism well-known for its capacity to form biofilms, is an opportunistic pathogen and is one of the top three causes of opportunistic human infections, 6 causing nosocomial infections in catheter lines, or chronically infecting the lungs of cystic fibrosis patients. (rsc.org)
Being an opportunistic pathogen that affects immunosuppressed people, P. aeruginosa has great clinical importance. (usp.br)

Bacteria within biofilms are protected from external damage and attacks from the host immune system and can exchange genomic information including antibiotic-resistance genes. (brad.ac.uk)
Understanding how bacteria develop biofilms is pertinent to tackle biofilm-associated infections and transmission. (brad.ac.uk)
While free-floating planktonic bacteria can be properly cleared from the CF lung, P. aeruginosa , along with alginate production, establishes an infection in the form of a biofilm which supports its survival in nature and in vivo . (marshall.edu)
The present authors hypothesised that bronchoscopy with protected specimen brush may sample biofilm-forming bacteria adherent to the airway wall, whereas traditional sputum collection may not. (ersjournals.com)
Retrieved bacteria were genotyped, and grown in planktonic cultures and as biofilms, and susceptibilities to individual antibiotics and to antibiotic combinations were determined. (ersjournals.com)
Susceptibility to single antibiotics and to antibiotic combinations were not different between planktonically or biofilm-grown bacteria derived from sputum, as compared to those obtained by bronchoalveolar lavage and protected brush. (ersjournals.com)
One problem with relying on sputum samples to guide therapy is that biofilm bacteria may not be adequately sampled via sputum, since sputum expectoration only samples P. aeruginosa that have detached and dispersed from the airway biofilm 5 , 6 . (ersjournals.com)
However, studies have not previously been done to determine whether bacteria retrieved from sputum are more susceptible to antibiotics compared to bacteria retrieved directly from the airway biofilm. (ersjournals.com)
Thus, it is possible that PSB may directly recover P. aeruginosa bacteria that have aggregated as biofilms and adhered to the surface of the airway. (ersjournals.com)
Kolter's research group was influential in the study of bacterial transport systems known as ABC exporters, published some of the earliest examples of experimental evolution through investigations of the stationary phase of bacterial growth, and was foundational in genetic studies of bacteria adhered to surfaces (living within communities called biofilms). (wikipedia.org)
The lab popularized the concept of bacterial biofilm formation as developmental or multicellular microbial processes, and pioneered genetic studies of cellular differentiation, signaling, and division of labor in bacteria. (wikipedia.org)
In our hospital, P. aeruginosa is one of the top coinfecting bacteria identified among COVID-19 patients. (frontiersin.org)
Bacteria have evolved multiple strategies for causing infections that include producing virulence factors, undertaking motility, developing biofilms and invading host cells. (nature.com)
Preincubation of bacteria with P. aeruginosa-conditioned (24 h) medium completely prevented the binding of SM23 to the cells. (unimore.it)
Quorum sensing plays a crucial role in the virulence nature of P. aeruginosa as it helps the bacteria to produce virulence factors and biofilm formation. (ijpsr.com)
[13] The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
[8] [5] The first colonist bacteria of a biofilm may adhere to the surface initially by the weak van der Waals forces and hydrophobic effects. (wikipedia.org)
Bacteria often switch from a free-living lifestyle to a surface adapted, structured lifestyle known as a biofilm. (southampton.ac.uk)
We found that subpopulations of biofilm bacteria undergo cell death and lysis as a feature of the Pseudomonas aeruginosa biofilm life cycle. (southampton.ac.uk)
We isolated and named this phage, showed that it becomes superinfective to its host during biofilm development, and found that it plays a role in phenotypic diversification of biofilm bacteria. (southampton.ac.uk)
Biofilm is a complex matrix consisting of extracellular polysaccharides, DNA, and proteins that protect bacteria from a variety of physical, chemical, and biological stresses allowing them to survive in hostile environments. (intechopen.com)
Bacteria can be found in planktonic form or in specific conditions, as sessile aggregates on both biotic and abiotic surfaces originating complex structures known as biofilm. (intechopen.com)
The biofilm matrix consists of polysaccharides, proteins, and DNA and constitutes a stubborn source that protects bacteria from a variety of physical, chemical, and biological stresses. (intechopen.com)
1,2 The unique structure of biofilms protects bacteria from the surrounding environment, conferring a capacity for persistence against phagocytosis, oxidative stresses, nutrient/oxygen restriction, metabolic waste accumulation, interspecies competition, and conventional antimicrobial agents. (rsc.org)
2,9-11 A comprehensive understanding of the interactions between bacteria and materials with different surface topographies may pave the way for more effective strategies to control biofilm growth. (rsc.org)
An important factor of bacterial virulence is the formation of biofilms which are aggregates of microorganisms incorporated into an extracellular matrix that protects bacteria from hostile environments. (usp.br)
Because of the potential to form biofilm, CDC recommends that all dental unit waterlines be treated regularly with disinfectants to meet the Environmental Protection Agency (EPA) regulatory standards for drinking water (i.e., ≤500 colony forming units (CFU)/mL of heterotrophic water bacteria). (cdc.gov)
This power low behavior may benefit bacteria social organization during biofilm formation. (wisc.edu)
In addition, bacteria form a biofilm on and within the endotracheal tube that protects them from antibiotics and host defenses. (msdmanuals.com)

The aims of this study were to investigate the prevalence of integrons and biofilm formation among P. aeruginosa and A. baumannii isolates collected from ICU and non-ICU inpatients. (magiran.com)
A total of 90 P. aeruginosa and 90 A. baumannii isolates were recovered from patients admitted into diverse units of Shahid Mohammadi hospital in Bandar Abbas from January to December 2014. (magiran.com)
Class 1 integron was detected in 13.3% of the P. aeruginosa and 40% of the A. baumannii isolates, while Class 2 integron was harbored by 7 and 6.6% of the isolates, respectively. (magiran.com)
Furthermore, 23% of the A. baumannii and 12% of the P. aeruginosa isolates showed strong biofilm activity. (magiran.com)
Class 1 integron-positive isolates were resistant to three classes of antibiotics and predominantly observed in specimens collected from ICU patients showing strong biofilm. (magiran.com)
The ability of isolates to form in vitro biofilm was detected by tube method followed by investigating the effect of each acetic and citric acid on biofilm formation. (alexjvs.com)
In summary, we demonstrated that P. aeuginosa clinical isolates with novel epigenetic markers could form excessive biofilm, which might enhance its antibiotic resistance and in vivo colonization in COVID-19 patients. (frontiersin.org)
Nevertheless, P118 isolates presented fluorescent cell agglomerates, suggesting biofilm formation. (bvsalud.org)
While 9 of these 20 isolates were of mucoid colony morphotype, among the 40 biofilm negative isolates mucoid colony was detected in 16 of them. (omu.edu.tr)
Nine of these genotypes composed of biofilm positive isolates and 15 were biofilm negative ones. (omu.edu.tr)
Most of the biofilm positive strains belonged to K1 (n= 5) and K2 (n= 6) genotypes while biofilm negative isolates were in the L (n= 8) and O (n= 7) genotypes. (omu.edu.tr)
The data obtained from this study indicated that biofilm negative and positive P.aeruginosa isolates clustered in different groups. (omu.edu.tr)
Beginning in June 2017, the Texas Department of State Health Services asked clinical laboratories to voluntarily submit clinical P. aeruginosa isolates resistant to imipenem, meropenem, or doripenem to the Texas Department of State Health Services Laboratory for mechanism testing through the Antibiotic Resistance Laboratory Network ( https://www.cdc.gov/drugresistance/ar-lab-networks/domestic.html ). (cdc.gov)
The goal of this study was to characterize P. aeruginosa isolates and identify the prevalence rate of P. aeruginosa resistant to imipenem due to metallo-ßlactamase (MBL) genes. (japsonline.com)
A total of 74 P. aeruginosa isolates were obtained between November 2015 and April 2017 from Al-Azhar University Hospital, Assiut, Egypt. (japsonline.com)
This study included 74 P. aeruginosa isolates recovered from 412 clinical isolates taken from patients urine admitted to wards/intensive care units (ICUs) and who acquired nosocomial infections after excluding signs or symptoms of infection at the time of admission. (japsonline.com)
Carbapenem-resistant P. aeruginosa (CRPA) isolates in Idaho intensive care unit (ICU) room (room X), 4 months apart. (cdc.gov)

Infections caused by Pseudomonas aeruginosa or Acinetobacter baumannii are of greatest concern for hospitalized patients, particularly those in intensive care units (ICUs). (magiran.com)
In clinical settings, persistent presence of biofilms increases the risks of healthcare-associated infections and imposes huge healthcare and economic burdens. (brad.ac.uk)
Dispersed bacterial cells from attached biofilms on medical devices or host tissues may also serve as the origin of further infections. (brad.ac.uk)
Unrelated phenotypic differences in biofilm formation and lung colonization as well as variations in genome size, all within different sources of P. aeruginosa were discovered and could lead to variations in morbidity and mortality related to P. aeruginosa infections in CF. A key factor in alginate regulation is AlgU/AlgT, a sigma factor that also impacts the expression of many other involved proteins. (marshall.edu)
The proposed approach was applied to identify target genes to treat Pseudomonas aeruginosa infections. (psu.edu)
These findings provide insights into the underlying mechanisms of action of a cerPAC to restrict the virulence of P. aeruginosa and can have implications in the development of alternative approaches to control infections. (nature.com)
Discussion and Conclusions: taken together, our results indicate that boronic acid SM23, besides inhibiting beta-lactamase, can also act as potent inhibitor of QS in P. aeruginosa, suggesting that it may have a potential application in the prevention and treatment of biofilm-associated P. aeruginosa infections. (unimore.it)
Hospital infections associated with multidrug-resistant (MDR) Pseudomonas aeruginosa are a worldwide public health problem. (bvsalud.org)
Results: Eighty percent of the isolated samples (16/20) could form a biofilm, and most of these were isolated from wound infections. (ac.ir)
Pseudomonas aeruginosa is a frequent cause of respiratory infections in cystic fibrosis (CF) patients. (omu.edu.tr)
Bacterial biofilms play an important role in UTIs, responsible for persistent infections leading to recurrences and relapses. (mdpi.com)
Pseudomonas aeruginosa are quite a common cause of hospital-acquired infections. (japsonline.com)
Possession of nosocomial multi-drug resistant P. aeruginosa infections was significantly associated with past antibiotic therapy or catheterization (p (japsonline.com)
Furthermore, the ability of the pathogen to build biofilms and to persist e.g. in the lungs of cystic fibrosis (CF) patients facilitates the establishment of chronic infections, which are largely recalcitrant to antimicrobial therapies. (elifesciences.org)
3 Bacterial biofilms can cause persistent human infections and can foul the surface of medical devices. (rsc.org)
3,6 Biofilm formation by P. aeruginosa is responsible for antimicrobial tolerance and causes major problems for treatment of infections. (rsc.org)
3,8 Therefore, it is important to develop biomaterials that can control biofilm growth thereby reduce infections. (rsc.org)
Infections caused by Pseudomonas aeruginosa. (innspub.net)
On September 17, 2021, an Idaho hospital (hospital A) Treatment of carbapenemase-producing carbapenem-resis- collected sputum by endotracheal tube aspiration of a woman tant Pseudomonas aeruginosa (CP-CRPA) infections is chal- aged 50-65 years (patient 1), who received mechanical ven- lenging because of antibiotic resistance. (cdc.gov)
CP-CRPA infections tilation during 3 of 5 weeks of hospitalization in an intensive are highly transmissible in health care settings because they care unit (ICU) room (room X). Carbapenemase-producing can spread from person to person and from environmental carbapenem-resistant Pseudomonas aeruginosa (CP-CRPA) was sources such as sink drains and toilets. (cdc.gov)

Escherichia coli Nissle 1917 inhibits biofilm formation and mitigates virulence in Pseudomonas aeruginosa . (nih.gov)
Subsequent work showed similar behaviour as P. aeruginosa aligned within subcellular-nanogratings, 13 Escherichia coli orientated towards surface line patterns, 14 and Pseudomonas fluorescens were trapped preferentially in surface trenches. (rsc.org)

Prevention of the initiation of biofilm formation is the most important step for combating biofilm-associated pathogens, as the ability of pathogens to resist antibiotics is enhanced 10 to 1000 times once biofilms are formed. (psu.edu)
Genes essential to bacterial growth in the planktonic state are potential targets to treat biofilm-associated pathogens. (psu.edu)
Pseudomonas aeruginosa is one of the most emerging multidrug-resistant opportunistic pathogens, which poses a threat to immunocompromised patients. (ijpsr.com)
Because aerobic gram-negative bacilli (eg, Pseudomonas aeruginosa) are the major pathogens associated with HAP, the pathophysiology of nosocomial pneumonia relates to the destructive effect on lung tissue. (medscape.com)
An association between periodontal pathogens, such as Porphyromonas gingivalis, and atherosclerosis has been suggested because of the pathogen's possible direct effect on atheroma formation. (medscape.com)

The viscoelastic properties of this biofilm contributes to its ability to evade phagocytic activities, to reduce the effectiveness of antibiotics, to develop antibiotic resistant strains, and to persist or reoccur in CF patients. (aiche.org)
However, the biofilm formation capability of strains with mutations in these essential genes must be evaluated, since the pathogen might form a biofilm before it is eliminated. (psu.edu)
A total of 10 patients (83%) had a single strain of P. aeruginosa found using sputum, bronchoalveolar lavage and protected brush techniques, and two patients (17%) had two strains recovered in sputum, but only one strain was recovered using bronchoscopic techniques. (ersjournals.com)
In this study, quantum dots (QDs) were used to evaluate the effect of carbonyl cyanide -3-chlorophenylhydrazone ( CCCP ), an efflux pump system inhibitor, on biofilm formation and antimicrobial resistance profile of P. aeruginosa strains . (bvsalud.org)
QDs conjugated to antimicrobials can be used as nanotools to investigate multidrug-resistant bacterial strains on biofilm formation. (bvsalud.org)
Treatment of P. aeruginosa in the affected patients has become difficult because of its ability to develop resistant strains against most of the conventional therapeutic antibiotics. (ijpsr.com)
P.aeruginosa strains isolated from these patients have often a mucoid phenotype at advanced disease. (omu.edu.tr)
The aims of this study were to investigate the biofilm formation and the relation of this property with genotype and antibiotic susceptibilities of P.aeruginosa strains isolated from CF patients. (omu.edu.tr)
We showed that strains of Pseudomonas aeruginosa with increased mutation rates (caused by deletion of genes involved in DNA mismatch repair) produced more biofilm biomass in laboratory experiments. (southampton.ac.uk)
About 69% of the strains were MDR, and 86.27% of the multi-drug resistant (MDR) strains were biofilm producers. (japsonline.com)
PCR showed that eight strains of imipenem-resistant P. aeruginosa contained bla VIM , while bla IMP gene was not detected. (japsonline.com)
This study aimed to isolate and identify P. aeruginosa from Al-Azhar University Hospital, identify the measurement of antibacterial activity of different groups of antibiotics on P. aeruginosa , determine the incidence of multi-drug resistant (MDR) P. aeruginosa in infected patients, and detect MBLs genes among MDR P. aeruginosa strains using polymerase chain reaction (PCR). (japsonline.com)
Any drug impairing crucial processes for bacterial life will inevitably lead to the development of drug-resistant strains, whereas the inhibition of biofilm formation might prevent the onset of bacterial resistance. (intechopen.com)
In this section, we will focus on proteins involved in biofilm formation as useful targets for the development of new drugs that can effectively and specifically impair biofilm formation with slight effects on cell survival, thus avoiding the generation of drug-resistant strains. (intechopen.com)
7 Additionally, P. aeruginosa is intrinsically resistant to a variety of antibiotics and disinfectants and multi-drug resistant (MDR) strains have been identified. (rsc.org)

Although anti-swarmers exhibit the potential to yield clinical benefits, it is important to be aware of possible implications regarding biofilm formation and antibiotic resistance. (cranberryinstitute.org)
In conclusion, sputum collection provides as much information as bronchoscopy for characterising the genotype and antibiotic susceptibility of chronic Pseudomonas aeruginosa infection in patients with stable cystic fibrosis. (ersjournals.com)
Moreover, this study demonstrates that the degrees of antibiotic resistance and biofilm production are directly linked. (japsonline.com)

In this study, well-defined tannins present in multiple plant materials (tannic acid (TA) and epigallocathecin gallate (EGCG)) and undefined cranberry powder (CP) were found to block swarming motility and enhance biofilm formation and resistance to tobramycin in Pseudomonas aeruginosa. (cranberryinstitute.org)
In contrast, gallic acid (GA) did not completely block swarming motility and did not affect biofilm formation or tobramycin resistance. (cranberryinstitute.org)
These data support the theory that nutritional conditions can elicit an inverse relationship between swarming motility and biofilm formation capacities. (cranberryinstitute.org)

However, this regimen is varied according to patient experience ( e.g. drug allergy and/or intolerance), clinician judgement and the antimicrobial susceptibility profile of the P. aeruginosa isolate. (ersjournals.com)
Hence, local irritating factors, particularly the dental bacterial biofilm, seem to have a critical role in the susceptibility to, and onset and progression of, periodontal disease 2 . (bvsalud.org)
Thus, the aim of this research was to determine whether yeast present in CVC colonizations previously exposed to cell- wall targeted antibacterials benefit from a reduction in susceptibility to fluconazole and voriconazole, facilitating their ability to form biofilms. (bvsalud.org)
The susceptibility to fluconazole and voriconazole and the biofilm formation of the yeasts were tested before and after exposure to the antibacterials. (bvsalud.org)
None of the antibacterials exerted a significant effect on the in vitro susceptibility of the yeasts to the antifungal agents or on their ability to form biofilms. (bvsalud.org)

An increase in bacterial mutation rate can promote biofilm formation. (southampton.ac.uk)
Scoffield JA, Duan D, Zhu F, Wu H . A commensal streptococcus hijacks a Pseudomonas aeruginosa exopolysaccharide to promote biofilm formation. (ohsu.edu)

Pseudomonas aeruginosa is the most common pathogen that chronically infects patients with cystic fibrosis (CF) 1 . (ersjournals.com)
A key component of colonization, biofilm formation, and protection of the opportunistic human pathogen Pseudomonas aeruginosa is the biosynthesis of the exopolysaccharide Psl. (rcsb.org)

The disruption of biofilm formation would have a beneficial impact on the treatment of diseased CF patient as it will make the infection more susceptible to antibiotics and host phagocytosis. (aiche.org)
When investigating potential applications of anti-swarming materials it is important to consider whether the lack of swarming corresponds with an enhanced sessile biofilm lifestyle and resistance to antibiotics. (cranberryinstitute.org)
In vitro studies have shown thatbiofilm-forming P. aeruginosa are significantly less susceptible to antibiotics compared to nonadherent forms 3 , 7 . (ersjournals.com)
Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)
This project will exploit novel nitric oxide based therapies to improve the effectiveness of antibiotics against Pseudomonas aeruginosa biofilms. (southampton.ac.uk)

Pseudomonas aeruginosa is one of the model microorganisms for studying bacterial population behaviors. (brad.ac.uk)
A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
Disease-causing microorganisms found in untreated dental unit water can include Legionella, Pseudomonas aeruginosa , and nontuberculous Mycobacteria (NTM). (cdc.gov)
Trichomonas tenax was found in 22.53% of the biofilm samples (16.66% from gingivitis, 41.67% from periodontitis and 41.67% from healthy patients) and 9.81% of the saliva samples (20% from gingivitis, 40% from periodontitis and 40% from healthy patients).The presence of these microorganisms was related to the type of periodontal disease (p=0.001), but not with age (p=0.178) or risk factors (p=0.194). (bvsalud.org)

In order to address this issue, this work proposes a systems-level approach to quantifying the biofilm formation capability of mutants to determine target genes that are essential for bacterial metabolism in the planktonic state but do not induce biofilm formation in their mutants. (psu.edu)
The changes of fluxes through the reactions associated with the genes positively related to biofilm formation are used as soft sensors in the flux balance analysis to quantify the trend of biofilm formation upon the mutation of an essential gene. (psu.edu)
The essential genes whose mutants are predicted not to induce biofilm formation are regarded as gene targets. (psu.edu)
[10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)
Mapping transcriptional regulation of biofilm-related genes promoters in Pseudomonas. (usp.br)
In this sense, we aim to characterize the behaviour of different promoters of genes involved in biofilm regulation under different conditions and to search for possible new DNAbinding proteins associated with their promoter regions. (usp.br)
Nesse sentido, objetivamos caracterizar o comportamento de diferentes promotores de genes envolvidos na regulação de biofilme de P. aeruginosa sob diferentes condições e buscar possíveis novas proteínas de ligação ao DNA associadas às suas regiões promotoras. (usp.br)

Finally, the SM23 significantly reduced P. aeruginosa biofilm and pyoverdine production on endotracheal tubes, an in vitro condition closely mimicking clinical settings. (unimore.it)
The in-vitro analysis showed a marked decrease in the production of virulence factors such as pyocyanin, rhamnolipids, protease, elastase, exopolysaccharides, HCN gas production and biofilm formation. (ijpsr.com)
Mutation in the gene encoding this regulator abolished the ability of P. aeruginosa to produce biofilms in vitro, without any effect on the planktonic growth. (diagenode.com)

During June 2017-November 2019, a total 36 patients with carbapenem-resistant Pseudomonas aeruginosa harboring Verona-integron-encoded metallo-β-lactamase were identified in a city in western Texas, USA. (cdc.gov)
Verona-integron-encoded metallo-β-lactamase-producing carbapenem-resistant Pseudomonas aeruginosa (VIM-CRPA) and other carbapenemase-producing organisms (CPOs) are emerging public health threats. (cdc.gov)

The goal of this study is to investigate the effects of certain natural quorum sensing inhibitors on biofilm formation. (aiche.org)
This study correlates the activity of the quorum sensing inhibitors with viscoelastic changes in the P. aeruginosa biofilm on the air-medium interface. (aiche.org)

Anisotropy also plays a large role in the formation of bacterial communities called biofilms. (wisc.edu)

Additional alginate loci were located within the P. aeruginosa PAO1 genome utilizing isogenic mucoid mutants coupled with PAO1-derived cosmid complementation. (marshall.edu)

P. aeruginosa small colony variants (SCVs) which prefer the biofilm mode of growth, thrive in harsh environmental conditions and are found in the chronically infected CF lung, were generated in the laboratory for further study. (marshall.edu)
We collected a strong biofilm-forming P. aeruginosa strain displaying small colony variant morphology from a severe COVID-19 patient. (frontiersin.org)
By using condensed matter techniques, we present for the first time the dynamics of colony formation at early stages of biofilm development for Pseudomonas aeruginosa. (wisc.edu)
The development of colony biofilms by Pseudomonas aeruginosa is affected by redox-active compounds called phenazines. (columbia.edu)

Pseudomonas aeruginosa obtained from sputum, bronchoalveolar lavage and protected brush, taken from the right upper lung bronchus of 12 adult patients with cystic fibrosis, were compared. (ersjournals.com)

It is also interesting to find that P. aeruginosa tends to survive the essential-gene mutation treatment by mainly enhancing fluxes through 8 metabolic reactions that regulate acetate metabolism, arginine metabolism, and glutamate metabolism. (psu.edu)

It is crucial to understand bacterial gene regulatory networks and decision-making processes for biofilm formation upon initial surface attachment. (brad.ac.uk)
It is interesting to find that most essential gene mutants exhibit high potential to induce the biofilm formation while most non-essential gene mutants do not. (psu.edu)
Differential gene expression analysis indicated that this isolate formed excessive biofilm by reducing flagellar formation (7.4 to 1,624.1 folds) and overproducing extracellular matrix components including CdrA (4.4 folds), alginate (5.2 to 29.1 folds) and Pel (4.8-5.5 folds). (frontiersin.org)
By investigating the transcriptional changes related to QS, we found that Pseudomonas exposure to SM23 caused a notable decrease in the levels of lasI and lasR gene expression. (unimore.it)
We demonstrate that upon hitting a host cell, motile Pseudomonas aeruginosa induce a specific gene expression program. (elifesciences.org)
Gene regulation network in Pseudomonas aeruginosa is complex. (diagenode.com)

2015. Emergence of carbapenemase-producing Pseudomonas aeruginosa and Acinetobacter baumannii in livestock animals in Lebanon. (innspub.net)

Activity of carbonyl cyanide-3-chlorophenylhydrazone on biofilm formation and antimicrobial resistance in Pseudomonas aeruginosa using quantum dots-meropenem conjugates as nanotools. (bvsalud.org)
The disk diffusion method was applied to determine antimicrobial resistance patterns according to the Clinical and Laboratory Standards Institute, and the microtiter plate assay was managed to explore the biofilm formation. (japsonline.com)

For this, QDs were covalently conjugated to meropenem (MPM) and incubated with a P. aeruginosa resistant isolate (P118) or a control sensitive strain (ATCC Pa27853). (bvsalud.org)
The addition of the CCCP changed the labeling profile of the resistant isolate, and the absence of agglomerates was observed, indicating no biofilm formation. (bvsalud.org)

Aggregating and forming biofilms on biotic or abiotic surfaces are ubiquitous bacterial behaviors under various conditions. (brad.ac.uk)
Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)

81% of P. aeruginosa isolateswere sensitive to piperacillin/tazobactam and ticarcillin, while 60% were resistant to third generation of cephalosporins. (magiran.com)
We verified that the CCCP efflux system inhibitor may contribute to P. aeruginosa resistant phenotype reduction for some antimicrobials. (bvsalud.org)

Biofilm quantification was done by microtiter method. (magiran.com)

The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)
Biofilms are an ensemble of microbial cells irreversibly associated with a surface and enclosed in an essentially self-produced matrix. (intechopen.com)
Bacterial cells can colonize surfaces and form biofilms that consist of microbial cells embedded in extracellular polymeric substances (EPS). (rsc.org)

Recent evidence suggests that P. aeruginosa may actually grow as dense communities of exopolysaccharide-encapsulated biofilms adherent to CF patients' airways 2 - 4 . (ersjournals.com)
The purpose of the present study was to determine whether the PSB can sample directly from the airway biofilm and, thus, more accurately represent the state of P. aeruginosa in CF patients' lungs. (ersjournals.com)
Methods: The P. aeruginosa samples were isolated from patients with different types of infection, including wound infection, septicemia, and urinary tract infection. (ac.ir)
Patients with bronchiectasis and chronic infection with Pseudomonas aeruginosa have more frequent pulmonary exacerbations and hospital admissions, and reduced quality of life and survival, than those who are free of P. aeruginosa infection [ 1 ]. (ersjournals.com)
Adult patients with bronchiectasis who underwent treatment aimed at P. aeruginosa eradication between January 1, 2007 and December 31, 2014 were identified from the clinical database. (ersjournals.com)
In order to select patients with recent acquisition of P. aeruginosa , we included only those who had never grown P. aeruginosa or those who were free of this bacterium for at least 2 years (and documented by five or more negative samples) before the eradication trial. (ersjournals.com)
Overall, the eradication success rate at 6 months was 52% (n=33), and 70% (n=23) of these patients remained P. aeruginosa free for at least 1 year. (ersjournals.com)
Inadequate drying of gastrointestinal endoscope working channels may promote microbial reproduction and biofilm formation, increasing the risk of infection in patients. (hindawi.com)
This study analyzed the incidence of Entamoeba gingivalis and Trichomonas tenax in samples of dental biofilm and saliva from patients with gingivitis / periodontitis and in healthy subjects. (bvsalud.org)
Biofilm and saliva samples were taken from 20 patients with gingivitis, 22 with periodontitis and 9 healthy individuals. (bvsalud.org)

[2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)
This can contribute to biofilm dispersal, and provides a mechanism for the release of extracellular DNA (eDNA) within biofilms. (southampton.ac.uk)

It may also invade and proliferate within heart and coronary artery endothelial cells, and, along with Streptococcus sanguis, it may also induce platelet aggregation associated with thrombus formation. (medscape.com)

Methicillin-sensitive S. aureus , Streptococcus pneumoniae , and Haemophilus influenzae are most commonly implicated when pneumonia develops within 4 to 7 days of hospitalization, whereas P. aeruginosa , MRSA, and enteric gram-negative organisms become more common with increasing duration of intubation or hospitalization. (msdmanuals.com)

Taken together, the phenotypic and genotypic analysis of the regulation of alginate may further lead to treatments to prevent biofilm formation and subsequent CF host colonization with P. aeruginosa . (marshall.edu)

This tolerance often links biofilms with persistent and chronic infection, and provides ideal conditions for the acquisition or evolution of AMR. (southampton.ac.uk)

In this study, we present a novel transcriptional regulator, PA1226, which modulates biofilm formation and virulence in P. aeruginosa. (diagenode.com)
Biofilm formation is a crucial and precisely regulated process at the transcriptional level. (usp.br)

In this study, we investigated the effects of SM23, a boronic acid, which was specifically designed as beta-lactamase inhibitor, on biofilm formation and virulence factor production by P. aeruginosa Material and Methods: the bioluminescent P. aeruginosa strain P1242 was employed. (unimore.it)
The biofilm formation was determined by using the Congo Red agar and Christensen methods. (omu.edu.tr)
The aim of this project is to study of species composition of biofilm communities by cultivation and molecular methods and assessment of the differences in poly-microbial communities in relation to therapy failure, length of insertion of catheter, its type and other factors including predominating microbial species. (muni.cz)
Two of the articles reported lack of drying of gastrointestinal endoscopes while the other ten reported residual droplets, microbial growth, and biofilm formation after different methods of drying. (hindawi.com)

Here, we investigated bacterial attachment, cell alignment and biofilm formation of Pseudomonas aeruginosa on periodic nano-pillar surfaces with different pillar spacing. (rsc.org)

Hence, we compared the biofilm reduction of these compounds and visualized their binding interactions to different pathways to hypothesize a possible mechanism of action associated with the biofilm reduction. (innspub.net)

Results: the SM23 significantly inhibited the development of biofilm and the production of virulence factors, as pyoverdines and elastase, without affecting bacterial growth. (unimore.it)

In addition to the pure science implications of these studies, a potential application of this would be to integrate such chips into medical devices that are common sites of biofilm formation, such as catheters, and then use the chips to limit bacterial colonization. (columbia.edu)

cerPAC reduced the production of QS-regulated virulence determinants and protected D. melanogaster from fatal infection by P. aeruginosa PA14. (nature.com)
4 found that P. aeruginosa PA14 tended to maximum their contact area with the surface, forming a spontaneous cell alignment between periodic nano-pillars with a post pitch of 2.2, 0.9 and 0.7 μm. (rsc.org)

One of its main virulence factors is its ability to form viscous, alginate-encased biofilms within the pulmonary mucus. (aiche.org)
[8] Biofilms can form on the teeth of most animals as dental plaque , where they may cause tooth decay and gum disease . (wikipedia.org)
A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)
We found that Pseudomonas aeruginosa does not follow an isotropic random walk as commonly assumed, but instead obeys a new form of polysaccharide-guided dynamics such that the distribution of surface visitation follows a power law. (wisc.edu)
This is an exciting new application for CMOS technology that will provide new insights into how biofilms form," says Shepard. (columbia.edu)

Biofilms have been suggested as a continuum of growth modes for adapting to different environments, initiating from bacterial cells sensing their attachment to a surface and then switching cellular physiological status for mature biofilm development. (brad.ac.uk)
This mucoid structure contains a dense amount of alginate type polysaccharide which facilitates bacterial attachment to lung epithelia and provides protection from the immune system due to biofilm formation. (omu.edu.tr)
Biofilm formation requires three different stages: cell attachment to a solid substrate, adhesion, and growth. (intechopen.com)
Surface topography designed to achieve spatial segregation has shown promise in delaying bacterial attachment and biofilm growth. (rsc.org)
In particular, surface modifications that physically create rational surface topographies have attracted attention in recent years, and have shown to inhibit bacterial attachment and biofilm growth without the use of antimicrobials. (rsc.org)

Inhibiting QS-controlled virulence factors without affecting the growth of P. aeruginosa may represent a promising strategy for overcoming its widespread and constantly increasing drug-resistance. (unimore.it)
Biofilms are increasingly recognised as the predominant mode of bacterial growth including within medical, engineered, and environmental contexts. (southampton.ac.uk)
20 generations, a few days of biofilm growth) within biofilms. (southampton.ac.uk)

npj Biofilms and Microbiomes , 9 (1). (brunel.ac.uk)
npj Biofilms and Microbiomes , 8 (1). (brunel.ac.uk)

Anatomy1

Organisms11

Diseases2

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes12

Information Science1

Opportunistic pathogen8

Bacteria30

Isolates17

Infections20

Escherichia2

Pathogens5

Strains15

Antibiotic3

Swarming motility3

Susceptibility5

Promote biofilm formation2

Pathogen2

Antibiotics5

Microorganisms6

Genes7

Vitro3

Carbapenem-resistant2

Quorum sensing in2

Communities called biofilms1

PAO11

Colony4

Cystic fibrosis1

Mutation1

Gene6

Acinetobacter1

Antimicrobial resistance2

Isolate2

Biotic2

Resistant2

Quantification1

Microbial cells3

Patients10

Extracellular2

Induce1

Streptococcus1

Phenotypic1

Persistent1

Transcriptional2

Methods4

Surfaces1

Mechanism of act1

Significantly1

Colonization1

PA142

Form5

Attachment5

Growth3

Microbiomes2