Pseudomonas aeruginosa aeruginosa biofilms. Medical search. Frequent questions

5.1 Vegetative biofilm bacteria are phenotypically different from suspended planktonic cells of the same genotype. (astm.org)
The present authors hypothesised that bronchoscopy with protected specimen brush may sample biofilm-forming bacteria adherent to the airway wall, whereas traditional sputum collection may not. (ersjournals.com)
Retrieved bacteria were genotyped, and grown in planktonic cultures and as biofilms, and susceptibilities to individual antibiotics and to antibiotic combinations were determined. (ersjournals.com)
Susceptibility to single antibiotics and to antibiotic combinations were not different between planktonically or biofilm-grown bacteria derived from sputum, as compared to those obtained by bronchoalveolar lavage and protected brush. (ersjournals.com)
One problem with relying on sputum samples to guide therapy is that biofilm bacteria may not be adequately sampled via sputum, since sputum expectoration only samples P. aeruginosa that have detached and dispersed from the airway biofilm 5 , 6 . (ersjournals.com)
However, studies have not previously been done to determine whether bacteria retrieved from sputum are more susceptible to antibiotics compared to bacteria retrieved directly from the airway biofilm. (ersjournals.com)
Thus, it is possible that PSB may directly recover P. aeruginosa bacteria that have aggregated as biofilms and adhered to the surface of the airway. (ersjournals.com)
[13] The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
[8] [5] The first colonist bacteria of a biofilm may adhere to the surface initially by the weak van der Waals forces and hydrophobic effects. (wikipedia.org)
A biofilm is a structured consortium of bacteria, embedded in a self-produced polymer matrix consisting of polysaccharide, protein and DNA. (scienceopen.com)
The chronic inflammation is the major cause of the lung tissue damage in CF. Biofilm growth in CF lungs is associated with an increased frequency of mutations, slow growth and adaptation of the bacteria to the conditions in the lungs, and to antibiotic therapy. (scienceopen.com)
Conventional resistance mechanisms, such as chromosomal β-lactamase, upregulated efflux pumps, and mutations of antibiotic target molecules in the bacteria, also contribute to the survival of P. aeruginosa biofilms. (scienceopen.com)
Bacteria within biofilms are protected from external damage and attacks from the host immune system and can exchange genomic information including antibiotic-resistance genes. (brad.ac.uk)
Understanding how bacteria develop biofilms is pertinent to tackle biofilm-associated infections and transmission. (brad.ac.uk)
While free-floating planktonic bacteria can be properly cleared from the CF lung, P. aeruginosa , along with alginate production, establishes an infection in the form of a biofilm which supports its survival in nature and in vivo . (marshall.edu)
LxA 4 increased ciprofloxacin inhibition on biofilm formation but did not affect ciprofloxacin's action on non-adherent bacteria. (researchwithrowan.com)
Hydrophobicity can also affect the ability of bacteria to form biofilms. (wikipedia.org)
Flemming, H.-C. & Wuertz, S. Bacteria and archaea on Earth and their abundance in biofilms. (nature.com)
Preincubation of bacteria with P. aeruginosa-conditioned (24 h) medium completely prevented the binding of SM23 to the cells. (unimore.it)
An important factor of bacterial virulence is the formation of biofilms which are aggregates of microorganisms incorporated into an extracellular matrix that protects bacteria from hostile environments. (usp.br)
Quorum sensing plays a crucial role in the virulence nature of P. aeruginosa as it helps the bacteria to produce virulence factors and biofilm formation. (ijpsr.com)
Clinical isolates (n=30) of P. aeruginosa from CF patients were used to screen and evaluate a 17 novel anti-biofilm peptide candidate's activity (MIC/MBEC) in a high-throughput plate-based procedure, followed by confocal microscopy using live/dead bacteria staining. (edu.au)
Their ability to inhibit and disperse the bacterial biofilms in human primary airway epithelial cell cultures derived from the CF children were assessed using an air-liquid interface (ALI) cell culture biofilm model and GFP tag bacteria. (edu.au)
Here, novel short, synthetic cationic peptides were tested for their anti-biofilm effectiveness as well as their ability to inhibit and disperse P. aeruginosa biofilms.MethodClinical isolates (n=30) of P. aeruginosa from CF patients were used to screen and evaluate a 17 novel anti-biofilm peptide candidate's activity (MIC/MBEC) in a high-throughput plate-based procedure, followed by confocal microscopy using live/dead bacteria staining. (edu.au)
Biofilms form when groups of bacteria cover themselves in a sticky mixture of sugars, protein, and DNA. (the-scientist.com)
Within the biofilm, bacteria take on different responsibilities. (the-scientist.com)
Bacteria often switch from a free-living lifestyle to a surface adapted, structured lifestyle known as a biofilm. (southampton.ac.uk)
We found that subpopulations of biofilm bacteria undergo cell death and lysis as a feature of the Pseudomonas aeruginosa biofilm life cycle. (southampton.ac.uk)
We isolated and named this phage, showed that it becomes superinfective to its host during biofilm development, and found that it plays a role in phenotypic diversification of biofilm bacteria. (southampton.ac.uk)
In our hospital, P. aeruginosa is one of the top coinfecting bacteria identified among COVID-19 patients. (frontiersin.org)
Because of the potential to form biofilm, CDC recommends that all dental unit waterlines be treated regularly with disinfectants to meet the Environmental Protection Agency (EPA) regulatory standards for drinking water (i.e., ≤500 colony forming units (CFU)/mL of heterotrophic water bacteria). (cdc.gov)
Many antimicrobial agents that are effective against planktonic cells turn out to be ineffective against the same bacteria growing in a biofilm state 9,10 . (bvsalud.org)

In vitro studies have shown thatbiofilm-forming P. aeruginosa are significantly less susceptible to antibiotics compared to nonadherent forms 3 , 7 . (ersjournals.com)
The viscoelastic properties of this biofilm contributes to its ability to evade phagocytic activities, to reduce the effectiveness of antibiotics, to develop antibiotic resistant strains, and to persist or reoccur in CF patients. (aiche.org)
The disruption of biofilm formation would have a beneficial impact on the treatment of diseased CF patient as it will make the infection more susceptible to antibiotics and host phagocytosis. (aiche.org)
When investigating potential applications of anti-swarming materials it is important to consider whether the lack of swarming corresponds with an enhanced sessile biofilm lifestyle and resistance to antibiotics. (cranberryinstitute.org)
Class 1 integron-positive isolates were resistant to three classes of antibiotics and predominantly observed in specimens collected from ICU patients showing strong biofilm. (magiran.com)
Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)
Bacterial biofilms cause chronic infections because they show increased tolerance to antibiotics and resist phagocytosis, as well as other components of the innate and the adaptive immune system. (scienceopen.com)
This study aimed to isolate and identify P. aeruginosa from Al-Azhar University Hospital, identify the measurement of antibacterial activity of different groups of antibiotics on P. aeruginosa , determine the incidence of multi-drug resistant (MDR) P. aeruginosa in infected patients, and detect MBLs genes among MDR P. aeruginosa strains using polymerase chain reaction (PCR). (japsonline.com)
Prevention of the initiation of biofilm formation is the most important step for combating biofilm-associated pathogens, as the ability of pathogens to resist antibiotics is enhanced 10 to 1000 times once biofilms are formed. (psu.edu)
The serial passage of biofilms selected for biofilm hyperproduction which was characterised by increased biomass deposition and phenotypic diversification but was not associated with intrinsic resistance to antibiotics. (uea.ac.uk)
The mechanisms by which this was achieved appeared to be through constitutive increase of intracellular c-di-GMP concentrations via mutations in the phosphodiesterase dipA, the yfiBNR signalling complex and bifunctional diguanylate cyclase/ phosphodiesterase morA, Next, whether biofilm and planktonic lineages took disparate evolutionary trajectories to resistance to the antipseudomonal antibiotics, ciprofloxacin, ceftazidime and tobramycin was investigated. (uea.ac.uk)
Finally, the effect of adaptation to a panel of 48 antimicrobial agents including antibiotics, biocides and metals on biofilm formation, relative fitness and antimicrobial susceptibility was investigated. (uea.ac.uk)
P. aeruginosa readily forms biofilms which can reduce the efficacy of antibiotics used to eradicate the pathogen. (researchwithrowan.com)
This resistance has been partially attributed to periphery sequestration, where antibiotics fail to penetrate biofilm cell clusters. (bvsalud.org)
Treatment of P. aeruginosa in the affected patients has become difficult because of its ability to develop resistant strains against most of the conventional therapeutic antibiotics. (ijpsr.com)
Biofilm formation is typically resistant to antibiotics and no effective therapy has been developed yet for their treatment. (edu.au)
Six (HDP- 25,26,43,101,102,103) candidates were was found to exhibit anti-microbial, anti-attachment and anti-biofilm activity at 8-16 μg/mL compared with current conventional antibiotics (Amikacin 128-512 μg/mL/ Tobramycin 128-1024 μg/mL/ Ciprofloxacin 128-512 μg/mL). (edu.au)
Biofilms are home to millions of microbes, but disrupting their interactions could produce more effective antibiotics. (the-scientist.com)
This project will exploit novel nitric oxide based therapies to improve the effectiveness of antibiotics against Pseudomonas aeruginosa biofilms. (southampton.ac.uk)

Although M. avium has been detected in biofilms in in situ and laboratory models, information available on M. avium is limited compared to biofilm model species such as Pseudomonas aeruginosa, Escherichia coli, Staphylococcus and Streptococcus. (montana.edu)

abstract = "The aim of this study was to evaluate the bactericidal effect of calcium oxide (CaO) against Pseudomonas aeruginosa biofilms on quail eggshells and major egg contacting surfaces (stainless steel, plastic, and rubber). (elsevierpure.com)

A total of 10 patients (83%) had a single strain of P. aeruginosa found using sputum, bronchoalveolar lavage and protected brush techniques, and two patients (17%) had two strains recovered in sputum, but only one strain was recovered using bronchoscopic techniques. (ersjournals.com)
The persistence of chronic Pseudomonas aeruginosa lung infections in cystic fibrosis (CF) patients is due to biofilm-growing mucoid (alginate-producing) strains. (scienceopen.com)
Using various genetically mutated P. aeruginosa strains, it is observed that the contributions of the exopolysaccharides Pel, Psl, and alginate to biofilm viscoelasticity were different for the biofilms grown in vitro and in vivo. (tdl.org)
About 69% of the strains were MDR, and 86.27% of the multi-drug resistant (MDR) strains were biofilm producers. (japsonline.com)
PCR showed that eight strains of imipenem-resistant P. aeruginosa contained bla VIM , while bla IMP gene was not detected. (japsonline.com)
However, the biofilm formation capability of strains with mutations in these essential genes must be evaluated, since the pathogen might form a biofilm before it is eliminated. (psu.edu)
Influence of twitching and swarming motilities on biofilm formation in Pseudomonas strains. (helmholtz-hzi.de)
Upon E2 treatment, six out of 10 investigated CF isolates showed an increase of attached biofilm mass, whereas biofilms from two tested non-CF laboratory strains (PAO1 and ATCC19660) did not. (uni-siegen.de)
P.aeruginosa strains isolated from these patients have often a mucoid phenotype at advanced disease. (omu.edu.tr)
The aims of this study were to investigate the biofilm formation and the relation of this property with genotype and antibiotic susceptibilities of P.aeruginosa strains isolated from CF patients. (omu.edu.tr)
Most of the biofilm positive strains belonged to K1 (n= 5) and K2 (n= 6) genotypes while biofilm negative isolates were in the L (n= 8) and O (n= 7) genotypes. (omu.edu.tr)
We showed that strains of Pseudomonas aeruginosa with increased mutation rates (caused by deletion of genes involved in DNA mismatch repair) produced more biofilm biomass in laboratory experiments. (southampton.ac.uk)

A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
Pseudomonas aeruginosa is one of the model microorganisms for studying bacterial population behaviors. (brad.ac.uk)
Disease-causing microorganisms found in untreated dental unit water can include Legionella, Pseudomonas aeruginosa , and nontuberculous Mycobacteria (NTM). (cdc.gov)
Several studies focusing on the efficacy of mouthwashes with diverse chemical composition demonstrated that combination of sodium fluoride and sodium lauryl sulfate as well as essential oils is able to diminish the metabolic activity of microorganisms present in the dental biofilm 11-13 . (bvsalud.org)

A global genomic approach uncovers novel components for twitching motility-mediated biofilm expansion in Pseudomonas aeruginosa. (helmholtz-hzi.de)
P. aeruginosa twitching motility-mediated biofilm expansion is a coordinated, multicellular behaviour, allowing cells to rapidly colonize surfaces, including implanted medical devices. (helmholtz-hzi.de)
In the current study, we used a global genomics-based random-mutagenesis technique, transposon directed insertion-site sequencing (TraDIS), coupled with a physical segregation approach, to identify all genes implicated in twitching motility-mediated biofilm expansion in P. aeruginosa. (helmholtz-hzi.de)

One of its main virulence factors is its ability to form viscous, alginate-encased biofilms within the pulmonary mucus. (aiche.org)
Pseudomonas aeruginosa is an extremely successful pathogen able to cause both acute and chronic infections in a range of hosts, utilizing a diverse arsenal of cell-associated and secreted virulence factors. (helmholtz-hzi.de)
Introduction: Quorum sensing (QS) regulates the expression of virulence factors in P. aeruginosa. (unimore.it)
Inhibiting QS-controlled virulence factors without affecting the growth of P. aeruginosa may represent a promising strategy for overcoming its widespread and constantly increasing drug-resistance. (unimore.it)
The effect of the boronic acid SM23 on P. aeruginosa were assessed by evaluating a) the biofilm formation and its morphology by crystal violet staining/bioluminescence and confocal microscopy and b) the production in cell supernatant of the virulence factors, pyoverdines and elastase. (unimore.it)
Results: the SM23 significantly inhibited the development of biofilm and the production of virulence factors, as pyoverdines and elastase, without affecting bacterial growth. (unimore.it)
The in-vitro analysis showed a marked decrease in the production of virulence factors such as pyocyanin, rhamnolipids, protease, elastase, exopolysaccharides, HCN gas production and biofilm formation. (ijpsr.com)
These findings indicate a new anti-infective and alternative therapeutic approaches to combat the virulence factors of Pseudomonas aeruginosa and explore further the potential of fungal secondary metabolites from other saprophytic fungi colonizing decaying substrata of medicinal plants. (ijpsr.com)

Although anti-swarmers exhibit the potential to yield clinical benefits, it is important to be aware of possible implications regarding biofilm formation and antibiotic resistance. (cranberryinstitute.org)
Moreover, this study demonstrates that the degrees of antibiotic resistance and biofilm production are directly linked. (japsonline.com)
On September 17, 2021, an Idaho hospital (hospital A) Treatment of carbapenemase-producing carbapenem-resis- collected sputum by endotracheal tube aspiration of a woman tant Pseudomonas aeruginosa (CP-CRPA) infections is chal- aged 50-65 years (patient 1), who received mechanical ven- lenging because of antibiotic resistance. (cdc.gov)
In summary, we demonstrated that P. aeuginosa clinical isolates with novel epigenetic markers could form excessive biofilm, which might enhance its antibiotic resistance and in vivo colonization in COVID-19 patients. (frontiersin.org)

[10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)
The results demonstrate that mRNA levels for individual genes are not uniformly distributed throughout biofilms, but may vary by orders of magnitude over small distances. (montana.edu)
The goal of this study was to characterize P. aeruginosa isolates and identify the prevalence rate of P. aeruginosa resistant to imipenem due to metallo-ßlactamase (MBL) genes. (japsonline.com)
Genes essential to bacterial growth in the planktonic state are potential targets to treat biofilm-associated pathogens. (psu.edu)
In order to address this issue, this work proposes a systems-level approach to quantifying the biofilm formation capability of mutants to determine target genes that are essential for bacterial metabolism in the planktonic state but do not induce biofilm formation in their mutants. (psu.edu)
The changes of fluxes through the reactions associated with the genes positively related to biofilm formation are used as soft sensors in the flux balance analysis to quantify the trend of biofilm formation upon the mutation of an essential gene. (psu.edu)
The essential genes whose mutants are predicted not to induce biofilm formation are regarded as gene targets. (psu.edu)
The proposed approach was applied to identify target genes to treat Pseudomonas aeruginosa infections. (psu.edu)
This included efflux pump regulators, porins and biofilm-dependent pathways including type IV pili and alginate biosynthesis genes. (uea.ac.uk)
Finally, bioinformatic analysis shows the widespread abundance of cupE genes in isolates of P. aeruginosa and the co-occurrence of cupE with other cup clusters, suggesting interdependence of cup pili in regulating bacterial adherence within biofilms. (cam.ac.uk)
Our approach allowed identification of both known and novel genes, providing new insight into the complex molecular network that regulates this process in P. aeruginosa. (helmholtz-hzi.de)
Mapping transcriptional regulation of biofilm-related genes promoters in Pseudomonas. (usp.br)
In this sense, we aim to characterize the behaviour of different promoters of genes involved in biofilm regulation under different conditions and to search for possible new DNAbinding proteins associated with their promoter regions. (usp.br)
Nesse sentido, objetivamos caracterizar o comportamento de diferentes promotores de genes envolvidos na regulação de biofilme de P. aeruginosa sob diferentes condições e buscar possíveis novas proteínas de ligação ao DNA associadas às suas regiões promotoras. (usp.br)

Mutation in the gene encoding this regulator abolished the ability of P. aeruginosa to produce biofilms in vitro, without any effect on the planktonic growth. (diagenode.com)
The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)
Research has shown that M. avium cells in biofilms are more resistant to disinfectants than their planktonic counterparts. (montana.edu)
Biofilms represent a discrete form of microbial life which possess physiological distinctions from free-living planktonic counterparts which may also elicit lifestyle-dependent adaptive responses to selective pressures. (uea.ac.uk)
In fact, biofilms can withstand doses up to 1,000 times greater than their planktonic counterparts. (the-scientist.com)
Planktonic and biofilm cells also exhibit different susceptibilities to a certain antimicrobial concentration. (bvsalud.org)

In this study, well-defined tannins present in multiple plant materials (tannic acid (TA) and epigallocathecin gallate (EGCG)) and undefined cranberry powder (CP) were found to block swarming motility and enhance biofilm formation and resistance to tobramycin in Pseudomonas aeruginosa. (cranberryinstitute.org)
In contrast, gallic acid (GA) did not completely block swarming motility and did not affect biofilm formation or tobramycin resistance. (cranberryinstitute.org)
These data support the theory that nutritional conditions can elicit an inverse relationship between swarming motility and biofilm formation capacities. (cranberryinstitute.org)

Pseudomonas aeruginosa is the most common pathogen that chronically infects patients with cystic fibrosis (CF) 1 . (ersjournals.com)
Pseudomonas aeruginosa is an environmental opportunistic pathogen that is the main cause of morbidity in cystic fibrosis (CF) patients. (aiche.org)
It is also a relevant pathogen in chronic wounds infections and skin injuries , and in keratitis, a common corneal infection related with bacterial biofilm formation over contact lenses. (ibecbarcelona.eu)
It is important to understand their growth in mixed species biofilms, in order to begin to develop effective methods to both monitor and eventually control this opportunistic pathogen. (montana.edu)
P. aeruginosa , an opportunistic pathogen, establishes a chronic infection in CF with a phenotype of overproduction of an exopolysaccharide (alginate) due to host-directed mutagenesis. (marshall.edu)
In this study, we present the electron cryomicroscopy structure of the archaic CupE pilus from the opportunistic human pathogen Pseudomonas aeruginosa. (cam.ac.uk)
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen which is characterized by its ability to form biofilm, on biotic and abiotic surfaces, makes it particularly resistant to host's immune defenses and current antibiotic therapies. (alexjvs.com)
Being an opportunistic pathogen that affects immunosuppressed people, P. aeruginosa has great clinical importance. (usp.br)
Pseudomonas aeruginosa is a biofilm-forming opportunistic pathogen which causes chronic infections in immunocompromised patients and leads to high mortality rate. (frontiersin.org)
It is a biofilm-forming opportunistic pathogen causing life-threatening chronic infections in immunocompromised individuals with diseases like burn wounds, urinary tract infections, and respiratory infections ( Gellatly and Hancock, 2013 ). (frontiersin.org)

In conclusion, sputum collection provides as much information as bronchoscopy for characterising the genotype and antibiotic susceptibility of chronic Pseudomonas aeruginosa infection in patients with stable cystic fibrosis. (ersjournals.com)

Pseudomonas aeruginosa is one of the most emerging multidrug-resistant opportunistic pathogens, which poses a threat to immunocompromised patients. (ijpsr.com)

Biofilms grown in vivo are more relatively elastic than those grown in a wound-like medium in vitro but exhibited similar compliance. (tdl.org)
In vitro experiments with collagen containing medium suggest this likely arises from the incorporation of host material, most notably collagen, into the matrix of the biofilm when it is grown in vivo. (tdl.org)
Taken together with earlier studies that examined the in vitro effects of collagen on mechanical properties, we conclude that collagen may, in some cases, be the dominant contributor to biofilm viscoelasticity in vivo. (tdl.org)
The ability of isolates to form in vitro biofilm was detected by tube method followed by investigating the effect of each acetic and citric acid on biofilm formation. (alexjvs.com)
Finally, the SM23 significantly reduced P. aeruginosa biofilm and pyoverdine production on endotracheal tubes, an in vitro condition closely mimicking clinical settings. (unimore.it)
These P. aeruginosa cell clusters are in vitro models of the chronic P. aeruginosa infections in cystic fibrosis patients which display recalcitrance to antibiotic treatments , leading to exacerbated morbidity and mortality . (bvsalud.org)

Gallium therapy represents a viable therapeutic strategy for cystic fibrosis (CF) patients harbouring mucoid P. aeruginosa biofilm lung infections. (whiterose.ac.uk)
Siderophore deficient P. aeruginosa isolates still demonstrate a hindered biofilm proliferation when exposed to gallium but it is currently unknown whether exogenous gallium has any disruptive influence on the exopolysaccharide (EPS), the major mucoid P. aeruginosa CF lung biofilm matrix component. (whiterose.ac.uk)
Additional alginate loci were located within the P. aeruginosa PAO1 genome utilizing isogenic mucoid mutants coupled with PAO1-derived cosmid complementation. (marshall.edu)
There is growing evidence that blood serum concentrations of the steroid sex hormone estradiol (E2) correlate with the occurrence of pulmonary exacerbations in CF but also play a role in the mucoid switch of P. aeruginosa. (uni-siegen.de)
This mucoid structure contains a dense amount of alginate type polysaccharide which facilitates bacterial attachment to lung epithelia and provides protection from the immune system due to biofilm formation. (omu.edu.tr)
While 9 of these 20 isolates were of mucoid colony morphotype, among the 40 biofilm negative isolates mucoid colony was detected in 16 of them. (omu.edu.tr)

The microdilution method was used to evaluate the minimum inhibitory concentration (MIC) of this extract on P. aeruginosa, and biofilm inhibition was assayed. (ac.ir)
In addition to this, a significant inhibition in the motility factors of P. aeruginosa such as swimming, swarming, twitching was also observed. (ijpsr.com)

In sequential inoculation experiments an established biofilm of P. aeruginosa did not prevent biofilm formation by M. avium. (montana.edu)
Taken together, the phenotypic and genotypic analysis of the regulation of alginate may further lead to treatments to prevent biofilm formation and subsequent CF host colonization with P. aeruginosa . (marshall.edu)

A new technique was used to measure the viscoelasticity of in vivo Pseudomonas aeruginosa biofilms. (tdl.org)
This was done through ex vivo microrheology measurements of in vivo biofilms excised from mouse wound beds. (tdl.org)
To our knowledge, this is the first time that the mechanics of in vivo biofilms have been measured. (tdl.org)

Hyperthermophilic archaeon Pyrobaculum calidifontis produce bundling pili which are homologous to the bacterial TasA filaments, a major component of the extracellular matrix in bacterial biofilms, which contribute to biofilm stability. (wikipedia.org)
This can contribute to biofilm dispersal, and provides a mechanism for the release of extracellular DNA (eDNA) within biofilms. (southampton.ac.uk)

The purpose of the present study was to determine whether the PSB can sample directly from the airway biofilm and, thus, more accurately represent the state of P. aeruginosa in CF patients' lungs. (ersjournals.com)
In CF lungs, the polysaccharide alginate is the major part of the P. aeruginosa biofilm matrix. (scienceopen.com)
These results should be supported with larger scale multi-center studies which may provide information about P.aeruginosa dynamics in CF lungs. (omu.edu.tr)
He used a tool known as parallel sequential fluorescence in situ hybridization (par-seqFISH) on populations of Pseudomonas aeruginosa, a species often found in the lungs of people with cystic fibrosis. (the-scientist.com)

Unrelated phenotypic differences in biofilm formation and lung colonization as well as variations in genome size, all within different sources of P. aeruginosa were discovered and could lead to variations in morbidity and mortality related to P. aeruginosa infections in CF. A key factor in alginate regulation is AlgU/AlgT, a sigma factor that also impacts the expression of many other involved proteins. (marshall.edu)
Women with cystic fibrosis (CF) have a significantly lower life expectancy compared to men, which is indicated by an earlier impairment of lung function due to chronic colonization with biofilm formed by Pseudomonas aeruginosa. (uni-siegen.de)
Pseudomonas aeruginosa biofilm colonization is associated with declining lung function in cystic fibrosis (CF) patients. (edu.au)

In this study, we established RAINBOW-seq and profiled the transcriptome of Escherichia coli biofilm communities with high spatial resolution and high gene coverage. (nature.com)

While classical CUP pili have been extensively characterized, little is known about so-called archaic CUP pili, which are phylogenetically widespread and promote biofilm formation by several human pathogens. (cam.ac.uk)
An increase in bacterial mutation rate can promote biofilm formation. (southampton.ac.uk)

A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic bacterium commonly found in wound infections and airways of cystic fibrosis patients. (researchwithrowan.com)
Pseudomonas aeruginosa is a gram-negative bacterium predominant in soil, vegetation, and water. (usp.br)
As the biofilm matures and the community needs change, an individual bacterium may take on new responsibilities. (the-scientist.com)

Pseudomonas aeruginosa is a frequent cause of respiratory infections in cystic fibrosis (CF) patients. (omu.edu.tr)

The goal of this study is to investigate the effects of certain natural quorum sensing inhibitors on biofilm formation. (aiche.org)
This study correlates the activity of the quorum sensing inhibitors with viscoelastic changes in the P. aeruginosa biofilm on the air-medium interface. (aiche.org)

Aim: The aim of this study was to investigate the metabolic activity of Streptococcus mutans biofilms after treatment with mouthwashes with different compositions. (bvsalud.org)
14 (2004) studied the effects of mouthwashes containing essential oils, triclosan, cetylpyridinium chloride and chlorhexidine against Streptococcus gordonii biofilms. (bvsalud.org)

A model for antibiotic accumulation in bacterial biofilm microcolonies utilizing heterogenous porosity and attachment site profiles replicated the periphery sequestration reported in prior experimental studies on Pseudomonas aeruginosa PAO1 biofilm cell clusters. (bvsalud.org)

Biofilm growth reactors are engineered to produce biofilms with specific characteristics. (astm.org)

Because aerobic gram-negative bacilli (eg, Pseudomonas aeruginosa) are the major pathogens associated with HAP, the pathophysiology of nosocomial pneumonia relates to the destructive effect on lung tissue. (medscape.com)

Gene cluster controlling conversion to alginate-overproducing phenotype in Pseudomonas aeruginosa: functional analysis in a heterologous host and role in the instability of mucoidy. (scienceopen.com)
Differential gene expression analysis indicated that this isolate formed excessive biofilm by reducing flagellar formation (7.4 to 1,624.1 folds) and overproducing extracellular matrix components including CdrA (4.4 folds), alginate (5.2 to 29.1 folds) and Pel (4.8-5.5 folds). (frontiersin.org)

Furthermore, 23% of the A. baumannii and 12% of the P. aeruginosa isolates showed strong biofilm activity. (magiran.com)

P. aeruginosa interacts with many respiratory viruses in chronic infections, including SARS-CoV-2, the causal agent of the COVID-19 pandemic. (ibecbarcelona.eu)
Biofilms can be prevented by early aggressive antibiotic prophylaxis or therapy, and they can be treated by chronic suppressive therapy. (scienceopen.com)
97% bio-volume reduction followed by induced disruption of the mature biofilms (ALI cultures), significant colony death (88-94%), 71% reduction in the production of the key chronic virulent factor pyocyanin, and 74% reduction of bacterial attachment to airway epithelial cells. (edu.au)
This tolerance often links biofilms with persistent and chronic infection, and provides ideal conditions for the acquisition or evolution of AMR. (southampton.ac.uk)

However the growth of P. aeruginosa in monospecies did not differ significantly from the dual species biofilms. (montana.edu)
Possession of nosocomial multi-drug resistant P. aeruginosa infections was significantly associated with past antibiotic therapy or catheterization (p (japsonline.com)
All the CaO treatments significantly reduced P. aeruginosa biofilms on all tested surfaces as compared to controls. (elsevierpure.com)

The gained new insight into the influence of steroid hormones on P. aeruginosa biofilm phenotypes might pave the way for novel future approaches in personalized medicine based on the patients' sex and hormonal status. (uni-siegen.de)

Here, novel short, synthetic cationic peptides were tested for their anti-biofilm effectiveness as well as their ability to inhibit and disperse P. aeruginosa biofilms. (edu.au)

Biofilms have been suggested as a continuum of growth modes for adapting to different environments, initiating from bacterial cells sensing their attachment to a surface and then switching cellular physiological status for mature biofilm development. (brad.ac.uk)
A major cell-associated virulence factor, the Type IV pilus (T4P), is required for epithelial cell adherence and mediates a form of surface translocation termed twitching motility, which is necessary to establish a mature biofilm and actively expand these biofilms. (helmholtz-hzi.de)

81% of P. aeruginosa isolateswere sensitive to piperacillin/tazobactam and ticarcillin, while 60% were resistant to third generation of cephalosporins. (magiran.com)
CP-CRPA infections tilation during 3 of 5 weeks of hospitalization in an intensive are highly transmissible in health care settings because they care unit (ICU) room (room X). Carbapenemase-producing can spread from person to person and from environmental carbapenem-resistant Pseudomonas aeruginosa (CP-CRPA) was sources such as sink drains and toilets. (cdc.gov)
Carbapenem-resistant P. aeruginosa (CRPA) isolates in Idaho intensive care unit (ICU) room (room X), 4 months apart. (cdc.gov)
In comparison of biofilm stability, the strongest and most resistant biofilm was formed on eggshell against the CaO treatment, followed by rubber, stainless steel, and plastic. (elsevierpure.com)

The control of the dental biofilm allows the maintenance of oral health and prevents the development of diseases such as caries and periodontal disease, which are strongly related to tooth loss [1]. (bvsalud.org)

The disk diffusion method was applied to determine antimicrobial resistance patterns according to the Clinical and Laboratory Standards Institute, and the microtiter plate assay was managed to explore the biofilm formation. (japsonline.com)
In this work, an experimental evolution model is validated and used to study the evolutionary trajectories to antimicrobial resistance in biofilms of Pseudomonas aeruginosa. (uea.ac.uk)

Pseudomonas aeruginosa obtained from sputum, bronchoalveolar lavage and protected brush, taken from the right upper lung bronchus of 12 adult patients with cystic fibrosis, were compared. (ersjournals.com)
Bacterial biofilms are formed by different species that colonize and grow together on human tissues, causing infections in the urinary tract, lung and kidney among others, and on surfaces of medical devices, as joint prostheses , heart valves and pacemakers, dental and breast implants, sutures and catheters. (ibecbarcelona.eu)
P. aeruginosa small colony variants (SCVs) which prefer the biofilm mode of growth, thrive in harsh environmental conditions and are found in the chronically infected CF lung, were generated in the laboratory for further study. (marshall.edu)

In clinical settings, persistent presence of biofilms increases the risks of healthcare-associated infections and imposes huge healthcare and economic burdens. (brad.ac.uk)
This study included 74 P. aeruginosa isolates recovered from 412 clinical isolates taken from patients urine admitted to wards/intensive care units (ICUs) and who acquired nosocomial infections after excluding signs or symptoms of infection at the time of admission. (japsonline.com)
Together, our results suggest the biofilm modulating effects of E2 on various clinical CF isolates that are documented by both biomass and ultrastructural changes of biofilms. (uni-siegen.de)

In this review, researchers decipher the implication of P . aeruginosa in biofilms and pave the way to find solutions in a near future. (ibecbarcelona.eu)

Biofilm population density is recorded as log 10 colony forming units per surface area. (astm.org)
We collected a strong biofilm-forming P. aeruginosa strain displaying small colony variant morphology from a severe COVID-19 patient. (frontiersin.org)
The biofilm detection limit was 10(3) colony-forming units (CFU) E. coli K12 mL(-1), but required a modified protocol, which included a bio-blocker Pseudomonas aeruginosa with ethylenediaminetetraacetic acid buffered to pH 5 prior to cell lysis/DNA extraction. (who.int)

PelA deacetylase activity is required for Pel polysaccharide synthesis in Pseudomonas aeruginosa. (scienceopen.com)

Infections caused by Pseudomonas aeruginosa or Acinetobacter baumannii are of greatest concern for hospitalized patients, particularly those in intensive care units (ICUs). (magiran.com)
2015. Emergence of carbapenemase-producing Pseudomonas aeruginosa and Acinetobacter baumannii in livestock animals in Lebanon. (innspub.net)

Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
Aggregating and forming biofilms on biotic or abiotic surfaces are ubiquitous bacterial behaviors under various conditions. (brad.ac.uk)

It is also interesting to find that P. aeruginosa tends to survive the essential-gene mutation treatment by mainly enhancing fluxes through 8 metabolic reactions that regulate acetate metabolism, arginine metabolism, and glutamate metabolism. (psu.edu)
Conclusions: The treatment of biofilms with mouthwashes containing 0.2% chlorhexidine or essential oils induced significant reduction in S. mutans metabolism. (bvsalud.org)

Biofilm quantification was done by microtiter method. (magiran.com)
For this purpose, 10 CF isolates of the respiratory tract derived from different CF patients have been treated with E2 in a microtiter plate biofilm model. (uni-siegen.de)

Here, we describe the use of laser capture microdissection microscopy (LCMM) combined with multiplex quantitative real time reverse transcriptase polymerase chain reaction (qRT-PCR) to isolate and quantify RNA transcripts from small groups of cells at spatially resolved sites within biofilms. (montana.edu)

In the bronchoalveolar lavage fluid, Legionella pneumophila serogroup-1, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Burkholderia cepacia and filamentous fungi Scedosporium apiospermum and Scedosporium prolificans were detected. (who.int)
RÉSUMÉ Une étude en Iraq visait à évaluer l'occurrence de Legionella pneumophila dans différentes sources d'eau potable dans le gouvernorat de Bassora ainsi que la sensibilité des isolats à plusieurs antibiotiques. (who.int)

Historically, multicellular bacterial communities, known as biofilms, have been thought to be held together solely by a self-produced extracellular matrix. (nature.com)
Biofilms are multicellular communities that were so far thought to be held together solely by a self-produced organic extracellular matrix. (nature.com)

In this study, we present a novel transcriptional regulator, PA1226, which modulates biofilm formation and virulence in P. aeruginosa. (diagenode.com)
By investigating the transcriptional changes related to QS, we found that Pseudomonas exposure to SM23 caused a notable decrease in the levels of lasI and lasR gene expression. (unimore.it)
Biofilm formation is a crucial and precisely regulated process at the transcriptional level. (usp.br)

The goal in biofilm research and efficacy testing is to choose the growth reactor that generates the most relevant biofilm for the particular study. (astm.org)
Here we show that E. faecalis inhibits P. aeruginosa growth within biofilms when iron is restricted. (biorxiv.org)
We find that E. faecalis ldh1 mutant fails to inhibit P. aeruginosa growth. (biorxiv.org)
Together, our results suggest that E. faecalis synergistically inhibit P. aeruginosa growth by decreasing environmental pH and L-lactate-mediated iron chelation. (biorxiv.org)
We find that E. faecalis modulates the microenvironment by exporting lactic acid which further chelates already limited iron, and also lowers the environmental pH to antagonize P. aeruginosa growth in iron-restricted conditions. (biorxiv.org)
Interest in the growth of M. avium in biofilms has increased in the last few years. (montana.edu)
The main objective of the present research was to study the growth of M. avium in biofilms in the presence of P. aeruginosa. (montana.edu)
P. aeruginosa reduced the growth of M. avium. (montana.edu)
The growth of M. avium and P. aeruginosa was similar whether they were inoculated as base or invading species. (montana.edu)
The density of P. aeruginosa remained higher than the density of M. avium in the dual species biofilm, likely due to the higher growth rate of P. aeruginosa compared to that of M. avium. (montana.edu)
Biofilms are increasingly recognised as the predominant mode of bacterial growth including within medical, engineered, and environmental contexts. (southampton.ac.uk)
20 generations, a few days of biofilm growth) within biofilms. (southampton.ac.uk)
Methods: S. mutans biofilms were growth on polystyrene plates during 18 h, washed with sterile saline and treated with the following mouthwashes during 1 min: Listerine®, Oral B®, Parodontax® and Periogard® with and without alcohol. (bvsalud.org)

Gene expression in biofilms is dependent on bacterial responses to the local environmental conditions. (montana.edu)
Most techniques for studying bacterial gene expression in biofilms characterize average values over the entire population. (montana.edu)
In this study, we examined the direct actions of LxA 4 and RvD 2 on P. aeruginosa biofilm formation and virulence gene expression. (researchwithrowan.com)
LxA 4 and RvD 2 reduced P. aeruginosa biofilm formation and virulence gene expression. (researchwithrowan.com)
LxA4 and RvD2 reduced P. aeruginosa biofilm formation and virulence gene expression. (researchwithrowan.com)
The raw data were deposited to the Gene Expression Omnibus ( GSE197541 for RAINBOW-seq data and GSE213531 for RNA-seq Δ sapD biofilm periphery and pyrimidine biosynthesis heterogeneity). (nature.com)

Anatomy5

Organisms20

Diseases13

Chemicals and Drugs76

Analytical, Diagnostic and Therapeutic Techniques and Equipment13

Phenomena and Processes31

Information Science3

Health Care2

Bacteria33

Antibiotics19

Staphylococcus1

ABSTRACT1

Strains12

Microorganisms6

Motility-mediated biofilm expansion3

Virulence Factors8

Antibiotic resistance4

Genes14

Planktonic6

Swarming motility3

Pathogen10

Susceptibility1

Multidrug-resistant1

Vitro6

Mucoid6

Inhibition2

Prevent biofilm formation2

Vivo3

Contribute to biofilm2

Lungs4

Colonization3

Escherichia1

Promote biofilm formation2

Bacterium4

Infections in cystic fibrosis1

Quorum sensing in2

Streptococcus2

PAO11

Produce biofilms1

Pathogens1

Alginate2

Isolates showed1

Chronic4

Significantly3

Phenotypes1

Anti-biofilm1

Mature biofilm2

Resistant4

Dental biofilm1

Antimicrobial resistance2

Lung3

Clinical3

Pave the way1

Colony3

Polysaccharide1

Acinetobacter2

Biotic2

Metabolism2

Microtiter2

Polymerase chain re1

Legionella2

Extracellular matrix2

Transcriptional3

Growth13

Gene expression6