• Proximal tubular epithelial insulin receptor mediates high-fat diet-induced kidney injury. (harvard.edu)
  • Here, we describe an advanced in vitro model consisting of fully polarized renal proximal tubular epithelial cells cultured in a microfluidic system. (scienceopen.com)
  • Normally, renal tubule epithelial cells of female rats and male and female mice do not have hyaline droplets. (nih.gov)
  • Alpha 2u-globulin nephropathy comprises a male-rat-specific syndrome that results in a morphologically variable spectrum of lesions that generally includes the presence of hyaline droplets, degeneration/necrosis and regeneration of tubule epithelial cells, and the presence of granular casts in the outer medulla in subchronic studies. (nih.gov)
  • Up to 40 leak-tight tubules were cultured on this platform that provides access to the basolateral as well as the apical side of the epithelial cells. (scienceopen.com)
  • The renal tubule portion that extends from the BOWMAN CAPSULE in the KIDNEY CORTEX into the KIDNEY MEDULLA. (harvard.edu)
  • The proximal tubule consists of a convoluted proximal segment in the cortex, and a distal straight segment descending into the medulla where it forms the U-shaped LOOP OF HENLE. (harvard.edu)
  • Overexpression of Nrf2 in Renal Proximal Tubular Cells Stimulates Sodium-Glucose Cotransporter 2 Expression and Exacerbates Dysglycemia and Kidney Injury in Diabetic Mice. (harvard.edu)
  • Genetic labeling of the proximal tubule with either the mT/mG "tomato" or the R26Fucci2aR (Fucci) cell cycle reporter mice allows us to follow proximal tubule-specific changes in cell cycle after renal injury. (vanderbilt.edu)
  • 1991. Hyaline droplet accumulation in kidney proximal tubules of mice with histiocytic sarcoma. (nih.gov)
  • kidneys from 6-week-old WT male mice and female mice (only female proximal tubules were included in this database) were perfused via the left ventricle with perfusion buffer (135 mM NaCl, 1 mM Na 2 HPO 4 , 1.2 mM Na 2 SO 4 , 1.2 mM MgSO 4 , 5 mM KCl, 2 mM CaCl 2 , 5.5 mM glucose, 5 mM HEPES, pH 7.4). (nih.gov)
  • kidneys were then sliced and dissociated in perfusion buffer supplemented with collagenase B. Microdissection were performed under the stereomicroscope equipped with on-stage cooling. (nih.gov)
  • Dr. Burg spends a large portion of the interview discussing and describing his work in renal physiology, including kidney tubules and their perfusion, eventually without collagenase. (nih.gov)
  • Nephrotoxicity Assessment with Human Kidney Tubuloids using Spherical Nucleic Acid-Based mRNA Nanoflares. (harvard.edu)
  • drug-induced nephrotoxicity is a major health concern that contributes to 25% of all cases of severe acute kidney failure. (nih.gov)
  • Nephrotoxicity and Kidney Transport Assessment on 3D Perfused Proximal Tubules. (scienceopen.com)
  • 3D proximal tubule-on-chip model derived from kidney organoids with improved drug uptake. (harvard.edu)
  • The recent discovery of kidney organoids allows the formation of nephron-like structures that recapitulate some of the characteristics of the glomerular environment 6 , but they have no or limited filtration activity and the deposition of a correct GBM has not been fully demonstrated yet. (nature.com)
  • Scholars@Duke publication: Functional Coupling of Human Microphysiology Systems: Intestine, Liver, Kidney Proximal Tubule, Blood-Brain Barrier and Skeletal Muscle. (duke.edu)
  • MPS models representing the major absorption, metabolism and clearance organs (the jejunum, liver and kidney) were evaluated, along with skeletal muscle and neurovascular models. (duke.edu)
  • Serious health effects (such as irreparable damage to the liver or kidneys, or birth defects) are not used as a basis for establishing MRLs. (cdc.gov)
  • The foetal organs primarily affected are the central nervous system, the kidney and ureter and the skeletal system. (europa.eu)
  • Proximal tubular suspensions were prepared from rabbit kidney cortex and incubated in acidic (A) or control (C) media (pH 6.9 vs 7.4, 5% CO2) for 2 h. (jci.org)
  • Kidney Tubules, Proximal" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • Effect of in vitro metabolic acidosis on luminal Na+/H+ exchange and basolateral Na+:HCO3- cotransport in rabbit kidney proximal tubules. (jci.org)
  • The aim of this study was to evaluate the role of the kidney in mediating the signals involved in adaptive changes in luminal Na+/H+ exchange and basolateral Na+:HCO3- cotransport systems in metabolic acidosis. (jci.org)
  • In addition, the transepithelial transport function from the basolateral to the apical side of the proximal tubule was studied. (scienceopen.com)
  • Despite a wide variety of causes including metabolic abnormalities, hypertension, autoimmunity, and genetic background, a common early pathologic hallmark of chronic kidney disease (CKD) is decreased glomerular filtration and loss of functional glomeruli 2 . (nature.com)
  • Without functional proximal tubules, the kidneys cannot produce urine (a condition called anuria). (medlineplus.gov)
  • Our results demonstrate that we developed a functional 3D perfused proximal tubule model with advanced renal epithelial characteristics that can be used for drug screening studies. (scienceopen.com)
  • Hyaline droplets are eosinophilic homogeneous cytoplasmic droplets that are normally present in the P2 segment of proximal tubule epithelia of young, mature male rats. (nih.gov)
  • Kidney core biopsy revealed interstitial edema, acute interstitial nephritis, and oxalate crystal nephropathy. (scienceopen.com)
  • Their blood pressure usually normalizes, but they quickly develop chronic kidney disease, which is characterized by reduced kidney function that worsens over time. (medlineplus.gov)
  • Even though some success in generating kidney structures has been described using conventional 2D or 3D culture systems (including spheroids and extracellular based gels 5 ), the results are still inconsistent. (nature.com)
  • In particular, kidney structures called proximal tubules are absent or underdeveloped. (medlineplus.gov)
  • He graduated from Harvard College in 1952 with an A.B. and Harvard Medical School in 1955 with an M.D. After his medical residency at Boston VA Hospital and an internship at Beth Israel Hospital, Dr. Burg came to the Laboratory of Kidney and Electrolyte Metabolism [LKEM] at NHLBI in 1957 as a postdoctoral fellow. (nih.gov)
  • Dr. Burg recounts how he came to work at NIH through the Public Health Service, joining the Laboratory of Electrolyte and Kidney Metabolism. (nih.gov)
  • Furthermore, cell-specific responses such as cell cycle dynamics in vivo has conventionally relied on indirect immunohistochemistry and proximal tubule markers that may be downregulated in injury. (vanderbilt.edu)
  • KIM-1 mediates fatty acid uptake by renal tubular cells to promote progressive diabetic kidney disease. (harvard.edu)
  • Renal tubular dysgenesis is a severe kidney disorder characterized by abnormal development of the kidneys before birth. (medlineplus.gov)
  • As a result, kidney development is impaired, leading to the features of renal tubular dysgenesis. (medlineplus.gov)
  • Proximal tubules in the kidney play a crucial role in reabsorbing and eliminating substrates from the body into the urine, leading to high local concentrations of xenobiotics. (scienceopen.com)
  • This protein also stimulates production of the hormone aldosterone, which triggers the absorption of salt and water by the kidneys. (medlineplus.gov)
  • Kidney function completely recovered after a short course of steroid therapy. (scienceopen.com)
  • Flow cytometry studies on injured kidney tubules are complicated by a low yield of nucleated single cells. (vanderbilt.edu)
  • We report a new tissue dissociation protocol for kidney with an early fixation step that greatly enhances the yield of single cells. (vanderbilt.edu)
  • After injury, mature terminally differentiated kidney cells dedifferentiate into more primordial versions of themselves and then differentiate into the cell types needing replacement in the damaged tissue Macrophages can self-renew by local proliferation of mature differentiated cells. (wikipedia.org)
  • We used the Fucci mouse in conjunction with FlowSight to identify a discrete polyploid population in proximal tubules after aristolochic acid injury. (vanderbilt.edu)
  • Medications cause renal disease by promoting various types of injury in the kidney. (scienceopen.com)
  • This graph shows the total number of publications written about "Kidney Tubules, Proximal" by people in Harvard Catalyst Profiles by year, and whether "Kidney Tubules, Proximal" was a major or minor topic of these publication. (harvard.edu)
  • This makes the proximal tubule a major target for drug toxicity that needs to be evaluated during the drug development process. (scienceopen.com)
  • These genes are involved in the renin-angiotensin system, which regulates blood pressure and the balance of fluids and salts in the body and plays a role in kidney development before birth. (medlineplus.gov)
  • Dr. Burg is a renal physiologist who has made significant contributions to the field and greatly aided the understanding of how the kidney functions in health and disease. (nih.gov)
  • A fast histochemical staining method to identify hyaline droplets in the rat kidney. (nih.gov)
  • Without this system, the kidneys cannot control blood pressure. (medlineplus.gov)
  • Association of Coding Variants in Hydroxysteroid 17-beta Dehydrogenase 14 (HSD17B14) with Reduced Progression to End Stage Kidney Disease in Type 1 Diabetes. (harvard.edu)