• The exceptionally low corrosion rate (∼0.1 mm y-1 in concentrated NaCl solution for 7 days) enables lean Mg-Ca alloys great potential for diverse applications, particularly if relevant properties (e.g. mechanical strength, electrochemical performance, etc.) can be enhanced by thermomechanical processing. (researchgate.net)
  • Supplied as a solution in HEPES, NaCl, DTT. (novusbio.com)
  • HEPES has been described as one of the best all-purpose buffers available for biological research. (sigmaaldrich.com)
  • At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. (sigmaaldrich.com)
  • How much is the buffer CAPS for electrophoresis? (whdsbio.cn)
  • After incubation with the enzyme remove the cover slips and place slides on platform in an electrophoresis tank that contains the prechilled (4°C for at least one hour) electrophoresis solution . (neb.com)
  • HEPES ( 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid ) is a zwitterionic buffer with CAS number 7365-45-9. (whdsbio.cn)
  • This buffer contains an optimal concentration of calcium that facilitates the binding of Annexin V to phosphatidylserine on the cell surface, which is essential for the detection of early-stage cell apoptosis by flow cytometry. (stemcell.com)
  • 6. Wash the cells once with HEPES buffer saline. (dojindo.com)
  • Wash slides in a staining jar with enzyme buffer 3 times, 5 minutes each wash. (neb.com)
  • Common Buffers and Solutions " has 4 results in Products. (rndsystems.com)
  • We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
  • This buffer is usually used in biochemical experiments. (whdsbio.cn)
  • HEPES finds its usage in in vitro experiments on Mg. (sigmaaldrich.com)
  • The final concentration of HEPES buffer is 10-50mmol/L. When applied to cell culture, the general culture medium contains 20mmol/L LHEPES can reach the buffering capacity. (whdsbio.cn)
  • Formulated into a ready-to-use buffer solution of a specific concentration. (whdsbio.cn)
  • To adjust the pH of the reaction system, this component is contained in virus preservation solutions or cell preservation solutions. (whdsbio.cn)
  • Prepare 1X enzyme reaction buffer . (neb.com)
  • Several organ-preservation solutions are available, and these are being constantly modified to provide improved organ storage and outcomes. (medscape.com)
  • This article discusses the pathophysiology and techniques of organ preservation and describes various preservation solutions currently used for kidney , liver , pancreas , small-bowel , lung , and heart transplantation . (medscape.com)
  • HEPES has been used in a wide variety of applications, including tissue culture. (sigmaaldrich.com)
  • 3. Dilute 1 mM Fura 2-AM DMSO solution with HEPES buffer saline to prepare 1 mM Fura 2-AM working solution. (dojindo.com)
  • HEPES is widely used in a variety of biochemical reactions and is used as a buffer reagent in some cell culture media. (whdsbio.cn)
  • It is commonly used to buffer cell culture media in air. (sigmaaldrich.com)
  • In open culture conditions, adding HEPES buffer can prevent the pH from oxidizing in the medium to increase, thereby maintaining the pH at about 7.0. (whdsbio.cn)
  • HEPES powder is resistant to high temperatures and has a melting point of up to 200°C, so it will not be degraded by autoclaving. (whdsbio.cn)
  • NP-EGTA AM can serve as a photolabile buffer in cells because, once converted to NP-EGTA by intracellular esterases, it will bind Ca 2+ with high affinity until photolyzed with UV light. (thermofisher.com)
  • 4. Remove the culture medium, and add 0.5 ml of the Fura 2-AM working solution to the cells. (dojindo.com)
  • It can not only be used as a buffer in the culture medium, but also as a pH regulator in cosmetics. (whdsbio.cn)
  • Then remove the Fura 2-AM working solution. (dojindo.com)
  • Remove cover slips from slides and place slides in a staining jar containing lysis buffer. (neb.com)
  • Cells were washed twice with phosphate buffered saline and then 0.3 ml of FBS and 0.2 ml of complexes were added gently to each well. (medscape.com)
  • Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. (fishersci.com)
  • HEPES is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. (sigmaaldrich.com)
  • For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly. (fishersci.com)
  • Aqueous buffered solution containing BSA and ≤0.09% sodium azide. (bdbiosciences.com)
  • 4. Ampicillin sodium salt: 200 mg/mL stock solution in water. (nih.gov)
  • HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors. (sigmaaldrich.com)
  • HEPES has been described as one of the best all-purpose buffers available for biological research. (sigmaaldrich.com)
  • HEPES has been used in a wide variety of applications, including tissue culture. (sigmaaldrich.com)
  • Depending on the ligand/analyte interaction study, the composition of the running buffer is expected to improve specific binding. (horiba.com)
  • Buffer composition, concentration or pH must be adjusted according to the biomolecules studied. (horiba.com)
  • Trypsin solution is extracted from pig pancreas and is effective for cell detachment and dissociation of diverse cell types. (genexisbiotech.com)
  • N -(2-hydroxyethyl) piperazine- N '-[2-ethanesulfonic acid] (HEPES) was obtained from Sigma (Madrid, Spain). (medscape.com)
  • It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. (sigmaaldrich.com)
  • It is commonly used to buffer cell culture media in air. (sigmaaldrich.com)
  • In addition, the Imaging Buffer included in this kit minimizes background fluorescence and maintains cell vitality while the assay is being performed. (dojindo.com)
  • It can neutralize small amounts of added acid or base, thus maintaining the pH of the solution relatively stable. (itwreagents.com)
  • Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step. (sigmaaldrich.com)
  • A buffer is a solution that can resist significant changes in pH levels upon the addition of small amount of acid or base. (itwreagents.com)
  • A buffer solution is a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid (see also more information on our acids & bases page ). (itwreagents.com)
  • Buffers can also be made by starting with just weak acid and then neutralizing a portion of that weak acid with a strong base (achieving the mixture of weak acid and its salt by neutralization). (itwreagents.com)
  • A buffer can resist pH change because the two components (conjugate acid and conjugate base) are both present in appreciable amounts at equilibrium and are able to neutralize small amounts of other acids and bases (in the form of H + and OH - ) when they are added to the solution. (itwreagents.com)
  • Acidic buffers are buffers that maintain a solution at pH below 7 and typically consist of a weak acid and one of its salts to provide the anion which acts as a base (its conjugate base). (itwreagents.com)
  • Basic buffers are buffers that regulate the pH around values ​​greater than 7 and typically consist of a weak base and a salt of its conjugated acid. (itwreagents.com)
  • They maintain a solution at pH value of around 7 and consist of polyprotic acid salts. (itwreagents.com)
  • The pH of a buffer solution depends on the dissociation constant, K a of the acid (or K b of the base) as well as on the ratio of acid (base) and its conjugate base (acid) concentrations. (itwreagents.com)
  • An antifungal antibiotic known amphotericin B solution can be added to cell culture medium. (genexisbiotech.com)
  • Cell culture medium can incorporate antibiotic/antimycotic solution. (genexisbiotech.com)
  • This dependence is described by the well-known Henderson-Hasselbalch equation which is often used in chemistry and biochemistry to perform the necessary calculations in the preparation of buffer solutions for use in the laboratory, or in other applications. (itwreagents.com)
  • Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. (fishersci.com)
  • No. 555670) and Protein G. Cells were incubated with FITC Annexin V in a buffer containing propidium iodide (PI) and analyzed by flow cytometry. (bdbiosciences.com)
  • Briefly, rat adipose cells were obtained from epididymal fat pads and suspended in Krebs-Ringer Hepes buffer with 5% bovine serum albumin at a lipocrit of 10% (final). (nih.gov)
  • After equilibration at 37°C for 30 min the cells were incubated for 30 min at 37°C with buffer (basal), 8 nM insulin, or 8 nM insulin plus peptide. (nih.gov)
  • Additionally, maintaining a constant temperature in the system and keeping the volume of solution in the imaging vessel constant will help with focus drift. (thermofisher.com)
  • This article discusses the pathophysiology and techniques of organ preservation and describes various preservation solutions currently used for kidney , liver , pancreas , small-bowel , lung , and heart transplantation . (medscape.com)
  • HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high solution clarity. (sigmaaldrich.com)
  • Elution at low pH (typically eluting directly into a solution of higher pH buffer to restore pH to an optimum value). (uni-konstanz.de)
  • HEPES is a biological buffer often referred to as a "Good′s" buffer. (sigmaaldrich.com)
  • HEPES Buffer Solution is a biological buffer being used cell culture medium (1M). (genexisbiotech.com)
  • SPGA-EDTA Solution is a suspending medium utilized for the extraction of cell-free viruses. (bioind.com)
  • The calibration solution helps correct for sensitivity dispersions on the sensor chip surface. (horiba.com)
  • At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. (sigmaaldrich.com)
  • 6. Isopropylthiogalactoside (IPTG): 1 M stock solution in water. (nih.gov)
  • 7. Dithiothreitol (DTT): 1 M stock solution in water. (nih.gov)