• Although over expressing any of these six single-repeat R3 transcription factors in Arabidopsis causes a glabrous phenotype, single mutants of these genes have different phenotypes. (biomedcentral.com)
  • The Arabidopsis mitogen-activated protein kinase (MAPK) kinase 2 (MKK2) and the downstream MAPKs MPK4 and MPK6 were isolated by functional complementation of osmosensitive yeast mutants. (edu.sa)
  • These similarities led us to test whether Arabidopsis hormone mutants could be used to assess allelic variation in the cognate Salix spp. (rothamsted.ac.uk)
  • Here we show that loss of function of Physcomitrella patens PpTON1 strongly affects development of the moss gametophore, phenocopying the developmental syndrome observed in Arabidopsis ton1 mutants: mutant leafy shoots display random orientation of cell division and severe defects in cell elongation, which are correlated with absence of PPB formation and disorganization of the cortical microtubule array in interphase cells. (silverchair.com)
  • The transient gene expression system using Arabidopsis mesophyll protoplasts has proven an important and versatile tool for conducting cell-based experiments using molecular, cellular, biochemical, genetic, genomic and proteomic approaches to analyze the functions of diverse signaling pathways and cellular machineries. (korea.ac.kr)
  • Plasmid Extracted with CommaPure® kits for Transfection of Arabidopsis Protoplasts. (biocomma.blog)
  • Test the CommaPure® Anion-exchange Plasmid Preparation kits for transfection of Arabidopsis protoplasts, and compare it with another famous brand anion-exchange kits. (biocomma.blog)
  • The method includes protoplast isolation, PEG-calcium transfection of plasmid DNA and protoplast culture. (korea.ac.kr)
  • The protoplast isolation and DNA transfection procedures take 6-8 h, and the results can be obtained in 2-24 h. (korea.ac.kr)
  • Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. (agrisera.com)
  • Comparative Analysis of MAMP-induced Calcium Influx in Arabidopsis Seedlings and Protoplasts. (mpg.de)
  • In the Arabidopsis genome, approximately 80 MAP3Ks (mitogen-activated protein kinase kinase kinases) have been identified. (molcells.org)
  • Take 100μg each of plasmid (35S : GFP) extracted from two Resin kits, and separate protoplasts from Arabidopsis thaliana by enzymatic hydrolysis, and transform foreign genes into protoplasts by PEG-mediated transformation for transient expression. (biocomma.blog)
  • Genome-wide analysis of basic leucine zipper transcription factor families in Arabidopsis thaliana, Oryza sativa and Populus trichocarpa , 2009, Journal of Shanghai University (English Edition). (github.io)
  • We also showed that OsPIF14 down-regulates OsDREB1B gene expression in rice protoplasts, corroborating the OsPIF14 repressor activity observed in the transactivation assays using Arabidopsis protoplasts. (unl.pt)
  • In contrast, our knowledge of the mechanisms behind axillary meristem regulation in Arabidopsis (Arabidopsis thaliana) has grown rapidly in the past few years through the exploitation of integrated physiological, genetic, and molecular assays. (rothamsted.ac.uk)
  • Here, we demonstrate that these assays can be directly transferred to study the control of bud activation in biomass willow and to assess similarities with the known hormone regulatory system in Arabidopsis. (rothamsted.ac.uk)
  • These results demonstrate that both knowledge and assays from Arabidopsis axillary meristem biology can be successfully applied to Salix spp. (rothamsted.ac.uk)
  • This study demonstrates that, in Arabidopsis roots, exogenous l-ascorbic acid triggers a transient increase of the cytosolic free calcium activity ([Ca2+]cyt. (deepdyve.com)
  • BoPID may have kinase activity based on the kinase phosphorylation prediction analysis of its sequence,its sequence is highly homologous with AtPID at the chromosome level and has strong species specificity when clustering with cruciferous plants such as turnip, Arabidopsis and Brassica napus in the phylogeny and collinearity analysis. (ahs.ac.cn)
  • Analysis of Transgenic Tobacco with Overexpression of Arabidopsis WUSCHEL Gene [J]. J Integr Plant Biol. (jipb.net)
  • 31. Ascencio-Ib ez JT, Sozzani R, Lee T, Chu T, Wolfinger RD, Cella R, Hanley-Bowdoin L: Worldwide analysis of Arabidopsis gene expression uncovers a complicated array of changes impacting pathogen response and cell cycle during geminivirus infection. (nicotinic-receptor.com)
  • Uncoupling of sustained MAMP receptor signaling from early outputs in an Arabidopsis endoplasmic reticulum glucosidase II allele. (mpg.de)
  • In Arabidopsis protoplasts, MKK2 was specifically activated by cold and salt stress and by the stress-induced MAPK kinase kinase MEKK1. (edu.sa)
  • After Plasmid preparation and transformation, the amount of protoplasts expressing the GFP green fluorescent protein in the plasmid extracted with the CommaPure® kit is significantly higher than that of Company W. (biocomma.blog)
  • She then shifted her work to studying protoplasts in Arabidopsis, a plant commonly used as a model system, and Sheen has developed the use of green fluorescent protein in higher plant research. (wikipedia.org)
  • We have now beforehand found a hyperlink between growth and a posh comprising the Arabidopsis caspase-like protease separase (additionally named EXTRA SPINDLE POLES [ESP]) and three Arabidopsis microtubule (MT)-based centromeric protein-E-like Kinesins 7 (KIN7), which belong to the so-called KIN7.3-clade (KIN7.1, KIN7.3, and KIN7.5). (keiseronlineuniversity.com)
  • Exogenous ascorbate also induces a moderate increase in programmed cell death symptoms in intact roots, but it does not activate Ca2+ influx currents in patch-clamped root protoplasts. (deepdyve.com)
  • Nutritional control of plant development: molecular analysis of the NO3- response pathway in Arabidopsis roots. (or.jp)
  • Here we show that exogenous abscisic acid and L-glutamate can also cause ATP accumulation around Arabidopsis thaliana roots. (edu.au)
  • RL-induced stomatal opening is not evident in epidermal peels of Arabidopsis plants 16 , 21 , but opening is conspicuous in the intact leaves. (nature.com)
  • Root epidermal protoplasts (expressing aequorin as a cytosolic free calcium reporter) can support an extracellular ADP-induced cytosolic calcium elevation in the presence of an extracellular reductant. (edu.au)
  • Accumulation of isochorismate-derived 2,3-dihydroxybenzoic 3-O-β-D-xyloside in Arabidopsis resistance to pathogens and ageing of leaves. (mpg.de)
  • We analyzed here the role of TRICHOMELESS2 (TCL2), a previously-uncharacterized single-repeat MYB transcription factor in trichome patterning in Arabidopsis . (biomedcentral.com)
  • A Ca2+-ATPase was purified from plasma membranes (PM) isolated from Arabidopsis cultured cells by calmodulin (CaM)-affinity chromatography. (unimi.it)
  • A Novel Fatty Acyl-CoA Synthetase Is Required for Pollen Development and Sporopollenin Biosynthesis in Arabidopsis. (mpg.de)
  • When it was too long, long pollen tubes formed and subsequently, large number of subprotoplasts instead of whole protoplasts were yielded, as the case reported by previous investigators. (jipb.net)
  • Lowering of the osmoticum concentration resulted in decrease of percentage of pollen protoplasts. (jipb.net)
  • Repression of sucrose/ultraviolet-B light-induced flavonoid accumulation in microbe-associated molecular pattern-triggered immunity in Arabidopsis. (mpg.de)
  • Zhang, Z. Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells. (uba.ar)
  • Rona, J.-P. 'Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells' (2005) Plant and Cell Physiology. (uba.ar)
  • Cho, M.H., Spalding, E.P., An anion channel in Arabidopsis hypocotyls activated by blue light (1996) Proc. (uba.ar)
  • At-ACA8 encodes a plasma membrane-localized calcium-ATPAse of arabidopsis with a calmodulin-binding domain at the N terminus / M.C. Bonza, P. Morandini, L. Luoni, M. Geisler, M.G. Palmgren, M.I. De Michelis. (unimi.it)
  • Cortical microtubules regulate cell expansion by determining cellulose microfibril orientation in the root apex of Arabidopsis thaliana . (biomedcentral.com)
  • note = "Funding Information: We thank G. Ammerer and all members of the Hirt laboratory for helpful suggestions and critical reading of the manuscript, A. Auer and A. Belokurow for technical assistance, and F. Lacroute for providing an Arabidopsis cDNA library for yeast complementation. (edu.sa)
  • A chemical screen for suppressors of the avrRpm1-RPM1-dependent hypersensitive cell death response in Arabidopsis thaliana. (mpg.de)
  • At-ACA8 (Arabidopsis-autoinhibited Ca2+-ATPase, isoform 8, accession no. (unimi.it)
  • The role of nitric oxide in the interaction of Arabidopsis thaliana with the biotrophic fungi, Golovinomyces orontii and Erysiphe pisi. (mpg.de)
  • When starting her own lab, she established a model system using plant protoplasts, cells which can be maintained easily in laboratory containers. (wikipedia.org)
  • All of the signaling components for the BL response exist in guard cells, and guard cell protoplasts (GCPs) swell in response to BL 12 . (nature.com)
  • 2014). In grapevine, initial studies to deliver the RNP are to date mostly limited to "naked" plant cells (protoplasts) (Malnoy et al. (oregonstate.edu)
  • Cell types for which generative models for at least some organelles have been built include human HeLa cells, mouse NIH 3T3 cells, Arabidopsis protoplasts and mouse T lymphocytes. (cellorganizer.org)
  • 27. Blomster T, Saloja�rvi J, Sipari N, BroscheM, Ahlfors R, Keina�nen M, Overmyer Kangasja�rvi J: Apoplastic reactive oxygen species transiently reduce auxin signaling and result in stress-induced morphogenic response in Arabidopsis. (nicotinic-receptor.com)
  • Plant natriuretic peptides (PNPs) are hormones that have been extracted from many different species, with the Arabidopsis thaliana PNP (AtPNP-A) being the most studied among them. (encyclopedia.pub)
  • Supported by a quantitative mathematical model, we characterize PULSE in protoplasts and achieved high induction rates, and we combined it with CRISPR-Cas9-based technologies to target synthetic signaling and developmental pathways. (uni-freiburg.de)
  • Jasmonates meet fatty acids: functional analysis of a new acyl-coenzyme A synthetase family from Arabidopsis thaliana. (mpg.de)
  • In main root protoplasts, the activity of both PLDα1 and NADPH oxidase increased under ABA treatment, and the NADPH oxidase activity increased much more in WT than in pldα1. (kib.ac.cn)
  • The synteny analysis showed that 11 orthologous pairs were identified between M. truncatula and Arabidopsis thaliana (A. thaliana) and 31 pairs between M. truncatula and Glycine max (G. max). (bvsalud.org)