Loading...
  • assays
  • With advances made in novel protoplast assays, taking full advantage of the completed plant genome sequences for functional genomic and proteomic analyses of individual plant genes and their products will become a reality. (plantphysiol.org)
  • freshly isolated
  • Freshly isolated cell suspension protoplasts of Solanum dulcamara were mixed with Agrobacterium rhizogenes , allowed to settle for 2 h, exposed to electrical pulses and further incubated for 2h. (springer.com)
  • Transformed colonies were not produced when freshly isolated protoplasts were mixed with Agrobacterium but not electroporated. (springer.com)
  • immunofluorescence
  • When exponentially growing yeast cells were treated sequentially with EDTA, beta-mercaptoethanol, and Zymolase to form protoplasts, a specific immunofluorescence signal was obtained with MAb 1.183. (asm.org)
  • bacterial
  • 19F NMR analysis of the antimicrobial peptide PGLa bound to native cell membranes from bacterial protoplasts and human erythrocytes. (sigmaaldrich.com)
  • For solid-state NMR analysis, we isolated native membranes from erythrocyte ghosts and bacterial protoplasts and prepared them as macroscopically oriented samples. (sigmaaldrich.com)
  • spherical
  • Protoplasts are spherical naked plant cells produced by the removal of the cell wall with digestive enzymes (Fig. 1). (springer.com)
  • The sub-protoplasts were spherical, so their two-dimensional areas (πr 2 ) were summed for each cell by the computer and multiplied by 4 to give the total surface area. (biologists.org)
  • seedlings
  • Both physical and physiological damages were observed as Al concentration increased, and apoptosis may have been mainly caused by Al present in the protoplasts of bamboo seedlings, as supported by microscopy evidence. (thefreedictionary.com)
  • cytoplasm
  • The red (R) and far-red (FR) light irradiation were shown to induce Ca 2+ concentration oscillations in cytoplasm of oat protoplasts. (spie.org)
  • uptake
  • In all situations tested, oligomycin inhibited respiratiory O-2 uptake indicating that respiration of mitochondria in protoplasts is not strictly ADP limited. (diva-portal.org)
  • electron
  • J. Burgess, P.J. Linstead and V.E.L. Fisher, Studies on higher plant protoplasts by scanning electron microscopy, Micron 8:113-122 (1977). (springer.com)
  • In scanning electron micrographs, the morphology of the extrusions of nonacclimated protoplasts fused with [LintdCho was virtually indistinguishable from that of the extrusions formed in acclimated protoplasts. (villanova.edu)
  • yeast
  • A monoclonal antibody that defines a surface antigen on Candida albicans hyphae cross-reacts with yeast cell protoplasts. (asm.org)
  • In a Western blot, MAb 1.183 showed reactivity with a 20-kDa protein in the sodium dodecyl sulfate extract from protoplasts, whereas no reactivity was found with cell wall material obtained from yeast cells. (asm.org)
  • respiration
  • Antimycin A inhibited respiration more in dark-adapted protoplasts than after illumination whereas SHAM gave the opposite response. (diva-portal.org)
  • Protoplasts from infected leaves showed a small initial increase in the cyanide (CN)-sensitive component (cytochrome pathway) of total respiration, but an increase in the CN-insensitive component (alternative pathway) was the major cause of the stimulation of total respiration. (lancs.ac.uk)
  • auxin
  • In the ABP1 -related mutants and Coimbra, no auxin-induced protoplast swelling occurred. (plantphysiol.org)
  • ABP1 seems to be involved in mediating rapid auxin-induced protoplast swelling, but it is not involved in the control of rapid auxin-induced growth. (plantphysiol.org)
  • The sensitivity of plant protoplasts to auxin can be characterized by the hormone concentration inducing the maximal variation of the transmembrane potential difference (ΔEm). (springer.com)
  • Ephritikhine G, Barbier-Brygoo H, Muller JF, Guern J (1987) Auxin effect on the transmembrane potential difference of wild-type and mutant tobacco protoplasts exhibiting a differential sensitivity to auxin. (springer.com)
  • Barbier-Brygoo H., Ephritikhine G., Klämbt D., Guern J. (1990) The Sensitivity of Plant Protoplasts to Auxin is Likely Modulated by the Number of Receptors at the Plasmalemma. (springer.com)