Bacterial metabolism selected lignin model compounds (vanillin, vanillate, 4- hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a Introduction culture collection. (lu.se)
Genome analyses revealed PcaQ-like DNA-binding sites encoded upstream of ABC transport systems in other members of the α-proteobacteria, and thus it appears likely that these systems are involved in the uptake of protocatechuate. (microbiologyresearch.org)
If you have used this database, please ensure that you acknowledge this most recent Pseudomonas Genome Database publication rather than just the website URL. (pseudomonas.com)
Draft whole-genome sequence of the antibiotic-producing soil isolate Pseudomonas sp. (paperbase.net)
Enzyme4
In enzymology, a protocatechuate 3,4-dioxygenase (EC 1.13.11.3) is an enzyme that catalyzes the chemical reaction 3,4-dihydroxybenzoate + O2 ⇌ {\displaystyle \rightleftharpoons } 3-carboxy-cis,cis-muconate Thus, the two substrates of this enzyme are 3,4-dihydroxybenzoate (protocatechuic acid) and O2, whereas its product is 3-carboxy-cis,cis-muconate. (wikipedia.org)
The systematic name of this enzyme class is protocatechuate:oxygen 3,4-oxidoreductase (decyclizing). (wikipedia.org)
The enzyme 4-hydroxybenzoate 3-monooxygenase transforms 4-hydroxybenzoate, NADPH, H+ and O2 into protocatechuate, NADP+ and H2O. (np-mrd.org)
Acid1
In further studies, the exceptional 4-sulfocatechol converting protocatechuate-3,4-dioxygenases from the sulphanilic acid degrading mixed culture were investigated. (uni-stuttgart.de)
Transport2
Results from electrophoretic mobility shift assays with purified PcaQ and from expression assays indicated that PcaQ activates expression of the transport system in the presence of protocatechuate. (microbiologyresearch.org)
These mutants grew poorly on protocatechuate, presumably because they fail to transport protocatechuate. (microbiologyresearch.org)
High1
ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. (lu.se)
Ring-cleavage2
Thereafter a dioxygenase catalyzes the formation of 4,5-dihydro-4,5-dihydroxyphthalate, which is oxidized by an NAD-dependent dehydrogenase to give 4,5-dihydroxyphthalate, Protocatechuate, formed by decarboxylation of 4,5-dihydroxyphthalate, is the substrate for ring cleavage enzymes. (nih.gov)
Isophthalate and terephthalate (anions) are readily used as carbon sources by aerobic bacteria, and preliminary evidence is consistent with catabolic routes for these isomers converging at the ring-cleavage substrate protocatechuate. (nih.gov)
Enzymology1
In enzymology, a protocatechuate 3,4-dioxygenase (EC 1.13.11.3) is an enzyme that catalyzes the chemical reaction 3,4-dihydroxybenzoate + O2 ⇌ {\displaystyle \rightleftharpoons } 3-carboxy-cis,cis-muconate Thus, the two substrates of this enzyme are 3,4-dihydroxybenzoate (protocatechuic acid) and O2, whereas its product is 3-carboxy-cis,cis-muconate. (wikipedia.org)
Degradation1
Comparative study on the degradation of dibutyl phthalate by two newly isolated Pseudomonas sp. (nih.gov)
their antibacterial activity is greatest against gram-positive organisms and poorest against Pseudomonas species. (medscape.com)
Sequences1
Two open reading frames of 417 and 906 base pairs were found that had no homology with previously reported sequences, including those encoding protocatechuate 3,4-dioxygenase. (nih.gov)