• The purpose of this study is to investigate the effect of metformin on c-myc expression and PCa progression. (nyu.edu)
  • Our results demonstrated that (i) in Hi-Myc mice that display murine prostate neoplasia and highly resemble the progression of human prostate tumors, metformin attenuated the development of prostate intraepithelial neoplasia (PIN, the precancerous lesion of prostate) and PCa lesions. (nyu.edu)
  • Review: In vitro glutathione S-transferase (GST) pull-down assay with recombinant GST-CCNT1, GST-CDK9, and GST-EAF1 together with His-tagged MYCN proteins demonstrated that MYCN directly binds to EAF1 with the highest affinity. (cusabio.com)
  • The c-myc protein is localized in the nucleus and can physically associate with RB protein in vitro, hence c-myc may functionally antagonize RB function. (wdv.com)
  • ii) Metformin reduced c-myc protein levels in vivo and in vitro. (nyu.edu)
  • c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I. (fhcrc.org)
  • Grandori, Carla and Gomez-Roman, Natividad and Felton-Edkins, Zoe A and Ngouenet, Celine and Galloway, Denise A and Eisenman, Robert N and White, Robert J (2005) c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I. Nature cell biology, 7 (3). (fhcrc.org)
  • c-Myc coordinates cell growth and division through a transcriptional programme that involves both RNA polymerase (Pol) II- and Pol III-transcribed genes. (fhcrc.org)
  • Here, we demonstrate that human c-Myc also directly enhances Pol I transcription of ribosomal RNA (rRNA) genes. (fhcrc.org)
  • Promoter methylation of p16 INK4A , c-myc and hMSH2 genes was assayed by methylation-specific PCR (MSP) and sequencing (mapping). (wjgnet.com)
  • Our data also show that c-Myc binds to specific consensus elements located in human rDNA and associates with the Pol I-specific factor SL1. (fhcrc.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • As activation of c-myc expression promotes cell proliferation and blocks differentiation, it may positively regulate cell growth. (wdv.com)
  • The up-regulation of proto-oncogene may be the consequence of epigenetic control of gene expression by demethylase, and mbd2 is involved in the regulation of hMSH2 expression in human gastric cancer. (wjgnet.com)
  • This Recombinant Human MYCN protein is an essential resource for cancer researchers. (cusabio.com)
  • Equipped with an N-terminal 6xHis-tag, our Recombinant Human MYCN protein facilitates effortless purification and detection. (cusabio.com)
  • Choose between liquid and lyophilized powder forms to best suit your experimental requirements and elevate your research with our Recombinant Human MYCN protein. (cusabio.com)
  • To explore the effect of DNA methyltransferase, demethylase and methyl-CpG binding protein MeCP2 on the expressions and methylation of hMSH2 and proto-oncogene in human gastric cancer. (wjgnet.com)
  • iv) Reduced PIN formation by metformin was associated with reduced levels of androgen receptor and proliferation marker Ki-67 in Hi-Myc mouse prostate glands. (nyu.edu)
  • The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. (cancerindex.org)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • Transcription levels of Dnmt1, mbd2, MeCP2, p16 INK4A , hMSH2 and c-myc were detected by using real-time PCR or RT-PCR. (wjgnet.com)
  • rRNA synthesis and accumulation occurs rapidly following activation of a conditional MYC-ER allele (coding for a Myc-oestrogen-receptor fusion protein), is resistant to inhibition of Pol II transcription and is markedly reduced by c-MYC RNA interference. (fhcrc.org)
  • N-myc proto-oncogene protein, also known as Class E basic helix-loop-helix protein 37 (bHLHe37), plays a critical role in cancer research. (cusabio.com)
  • The amplification of c-MYC oncogene plays a key role in early prostate epithelia cell transformation and PCa growth. (nyu.edu)
  • Replacement of wild-type RB can suppress the tumorigenicity of some of these cells, suggesting that the RB protein (Rb) may negatively regulate cell growth. (wdv.com)
  • Co-injection of c-myc, but not EJ-ras, c-fos or c-jun, inhibits the ability of Rb to arrest the cell cycle. (wdv.com)
  • Thus, c-myc and RB specifically antagonize one another in the cell. (wdv.com)
  • We propose that stimulation of rRNA synthesis by c-Myc is a key pathway driving cell growth and tumorigenesis. (fhcrc.org)
  • Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. (cusabio.com)
  • They all have an approved name which relates them back to a viral oncogene found in the genome of avian myelocyomatosis virus. (stackexchange.com)
  • Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. (lookformedical.com)
  • for example, protein kinase activation is part of the platelet activation signal pathway. (lookformedical.com)
  • Advance your cancer research with our high-quality Recombinant Human SRC protein, also known as Proto-oncogene tyrosine-protein kinase Src and Proto-oncogene c-Src. (cusabio.com)
  • Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. (icr.ac.uk)
  • Results: Activated zeta chain of T-cell receptor-associated protein kinase 70 (ZAP70) generated resistance to PI3K inhibition. (cancerdiagnosisprognosis.org)
  • These compensatory mechanisms include forkhead box O (FOXO) transcription factors along with mitogen-activated protein kinase (MAPK)-dependent pathways ( Cancer Discov 9(4) (2019)">11 , Cancer Cell 21(2) (2012)">12 ). (cancerdiagnosisprognosis.org)
  • As an example, Pim-1 serine/threonine kinase (PIM1) regulates cell proliferation and survival that might lead to reduction of therapeutic drug efficacy by promoting the Janus kinases/signal transducer and activator of transcription proteins (JAK/STAT) signaling pathway in MYC proto-oncogene (MYC)-driven cancer ( Nat Rev Cancer 11(1) (2011)">13 - Mol Cancer Ther 9(9) (2010)">16 ), particularly in breast cancer. (cancerdiagnosisprognosis.org)
  • Zeta chain of T-cell receptor associated protein kinase 70 (ZAP70) is a cytoplasmic tyrosine phosphoprotein. (cancerdiagnosisprognosis.org)
  • The RAS proto-oncogene (from rat sarcoma) was one of the earliest human oncogenes discovered and comprises at least three different biologically active genes as H-RAS, K-RAS and NRAS. (org.pk)
  • The MYCN protein regulates the activity of other genes by attaching (binding) to specific regions of DNA and controlling the first step of protein production (transcription). (medlineplus.gov)
  • The encoded DNA-binding protein regulates intestine-specific gene expression and enterocyte differentiation. (cancer-genetics.org)
  • These findings demonstrate that MAD1 regulates cell cycle withdrawal during a late stage of granulocyte differentiation, and suggest that the relative levels of MYC versus MAD1 mediate a balance between cell proliferation and terminal differentiation. (fhcrc.org)
  • Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. (icr.ac.uk)
  • Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. (icr.ac.uk)
  • Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. (icr.ac.uk)
  • Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. (icr.ac.uk)
  • Protein phosphorylation causes activation of signal transduction pathways, which are crucial for many biological processes ( J Am Acad Dermatol 53(2) (2005)">1 ). (cancerdiagnosisprognosis.org)
  • Most of these mutations lead to a premature stop signal in the instructions for making the protein. (medlineplus.gov)
  • Scope includes mutations and abnormal protein expression. (cancer-genetics.org)
  • Madam, A proto-oncogene is a normal gene that can become an oncogene due to mutations or increased expression. (org.pk)
  • The most well-characterized protein kinases in the human genome are protein serine/threonine kinases and protein tyrosine kinases ( Methods Enzymol 200 (1991)">2 ). (cancerdiagnosisprognosis.org)
  • Previous studies have identified the contribution of several activated tyrosine or serine/threonine kinases to growth compensation upon PI3K inhibition ( Otorhinolaryngol Head Neck Surg 1(2) (2016)">8 - Oncogene 30(22) (2011)">10 ). (cancerdiagnosisprognosis.org)
  • On the basis of this action, this protein is called a transcription factor. (medlineplus.gov)
  • Targeted disruption of the MYC antagonist MAD1 inhibits cell cycle exit during granulocyte differentiation. (fhcrc.org)
  • The switch from transcriptionally activating MYC-MAX to transcriptionally repressing MAD1-MAX protein heterodimers has been correlated with the initiation of terminal differentiation in many cell types. (fhcrc.org)
  • Proto-oncogene codes for proteins that assist regulate cell growth and differentiation through contribution in signal transduction and mitogenic signals concert, generally do it through their protein yield. (org.pk)
  • Available in both liquid and lyophilized powder forms, our Recombinant Human SRC protein is ready to support your cancer research endeavors. (cusabio.com)
  • Coordinated activation of candidate proto-oncogenes and cancer testes antigens via promoter demethylation in head and neck cancer and lung cancer. (org.pk)
  • Although cancer prognosis has historically been based on clinical and laboratory findings, analysis of the expression of various gene translocations and proteins has burgeoned as a method for determining prognosis. (arvojournals.org)
  • Our Recombinant Human SRC protein is produced using a robust E.coli expression system, providing a consistent and reliable source for your research needs. (cusabio.com)
  • This can be seen if you peruse the HUGO entries for the four MYC family members encoded in the human genome (extracts below). (stackexchange.com)
  • Thus c-myc was the first myc-related gene found in the human genome, and is the one that is responsible for Burkitt's lymphoma. (stackexchange.com)
  • The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (cusabio.com)
  • The v- and c- prefixes come from the early days of oncogene research. (stackexchange.com)
  • The full-length mature protein sequence (2-536aa) is N-terminal 10xHis-tagged to ensure efficient purification while maintaining native conformation and activity. (cusabio.com)
  • Studies in animals suggest that this protein is necessary for normal development of the limbs, heart, kidneys, lungs, nervous system, and digestive system. (medlineplus.gov)
  • The MYCN gene provides instructions for making a protein that plays an important role in the formation of tissues and organs during development before birth. (medlineplus.gov)