aaaaxxxProto oncogene proteins pp60 c src. Medical search. FAQ
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  • gene
  • This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. (cancerindex.org)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • Targeted disruption of the c-src gene leads to a severe form of osteopetrosis in mice . (naver.com)
  • As the c-src gene is expressed in all tissues and cells tested, we have analyzed fibroblasts from three individuals with malignant, congenital osteopetrosis for the expression of c-src at the protein level. (naver.com)
  • The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. (thermofisher.com)
  • Two transcript variants encoding the same protein have been found for this gene. (thermofisher.com)
  • Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma. (rush.edu)
  • For example, angiotensin II has been shown to stimulate VSMC growth, 2 3 increase the expression of enzymes that produce mediators of inflammation, such as phospholipase A 2 and NAD(P)H oxidase, 4 5 stimulate the JAK/STAT pathway, 6 and activate gene transcription of proto-oncogenes, such as c- fos . (ahajournals.org)
  • It was found that the v-src gene in RSV is required for the formation of cancer. (merckmillipore.com)
  • The characteristics of this gene, including the striking evolutionary conservation at the locus, suggest that the encoded protein may function in brain development. (uni-wuerzburg.de)
  • To obtain more information about the physiological role of the c-src gene in cells, we have studied differentiation-dependent alterations using the human HL-60 leukaemia cell line as a model system. (uni-wuerzburg.de)
  • tyrosine kinase, but not with increased c-src gene expression. (uni-wuerzburg.de)
  • 7 Cardiac hypertrophy is also characterized by the induction of immediate-early genes (eg, c- fos and c- jun ), activation of the fetal gene program (eg, ANF and skeletal α-actin), and increases in protein synthesis and cell size (reviewed in References 7 7 to 11). (ahajournals.org)
  • antibody
  • Therefore, the distribution of activated Src in fibrinogen-adherent platelets was determined by confocal microscopy using the anti-pTyr-418 antibody as a marker. (nih.gov)
  • Although this antibody may also react with the corresponding activation loop phosphotyrosine of other Src family members, it reacted on Western blots of platelet lysates with an apparent single band at ∼60 kD, consistent with Src (unpublished data). (nih.gov)
  • In contrast, the distribution of "total" Src determined with antibody 327 was more diffuse (Fig. 4). (nih.gov)
  • The following antibody was used in this experiment: Phospho-SRC (Tyr527) Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-17781, RRID AB_10980114. (thermofisher.com)
  • Anti-phospho-Src family (Tyr416) Antibody, clone 2N8 is an antibody against phospho-Src family (Tyr416) for use in WB. (merckmillipore.com)
  • Milli-MarkAnti-Src-FITC Antibody, clone GD11 detects level of Src & has been published & validated for use in FC. (merckmillipore.com)
  • Antibody recognizes Human Src. (merckmillipore.com)
  • homolog
  • Here, we identify a mouse homolog of the Drosophila Disabled (Dab) protein, mDab1, and show it is an adaptor molecule functioning in neural development. (embopress.org)
  • mRNA
  • Although copy number alterations showed strong cis- and trans-effects on mRNA abundance, relatively few of these extend to the protein level. (broadinstitute.org)
  • membrane
  • This NH2-terminal PH domain of Tiam1 can be functionally replaced by the myristoylated membrane localization domain of c-Src, indicating that the primary function of this PH domain is to localize the protein at the membrane. (nih.gov)
  • To determine whether membrane localization is the only function of the NH2-terminal PH domain, we fused the NH2terminal 20 amino acids of c-Src, containing the myristoylation site and a basic region, in front of a C580 Tiam1 protein, resulting in MS-C580 Tiam1 (see Materials and Methods). (nih.gov)
  • The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. (rush.edu)
  • proteomics data highlighted potential 20q candidates, including HNF4A (hepatocyte nuclear factor 4, alpha), TOMM34 (translocase of outer mitochondrial membrane 34) and SRC (SRC proto-oncogene, non-receptor tyrosine kinase). (broadinstitute.org)
  • C.-K. Huang, G.R. Laramee and J.E. Casnellie, Chemotactic factor induced tyrosine phosphorylation of membrane associated proteins in rabbit peritoneal neutrophils, Biochem. (springer.com)
  • The structural and functional competency of the UPIII (show UPK3A ELISA Kits )- Src (show SRC ELISA Kits ) signaling system in membrane microdomains is strictly regulated during oocyte maturation. (antibodies-online.com)
  • Proc
  • J.S. Gutkind and K.C. Robbins, Translocation of the FGR protein-tyrosine kinase as a consequence on neutrophil activation, Proc. (springer.com)
  • J. Gomez-Cambronero, M. Yamazaki, F. Metwally, T.F.P. Molski, V.A. Bonak, C.-K. Huang, E.L. Becker and R.I. Sha'afi, Granulocyte-macrophage colony-stimulating factor and human neutrophils: Role of guanine nucleotide regulatory proteins, Proc. (springer.com)
  • adaptor protein
  • In addition to known regulators of antigen receptor signaling, we identified a novel adaptor protein, SLAP-2 which shares 36% sequence similarity with the known Src-like adaptor protein, SLAP. (nih.gov)
  • Peptides
  • In the method of the invention, a protein kinase is contacted with an oriented degenerate peptide library, peptides within the library which are substrates for the kinase are converted to phosphopeptides and the phosphopeptides are separated from non-phosphorylated peptides. (patentgenius.com)
  • osteopetrosis
  • Thus, impairment of c-src function as an etiological factor in human osteopetrosis appears unlikely in the individuals investigated. (naver.com)
  • actin organization
  • It has been proposed that these proteins acting in concert to regulate actin organization and dynamics at this site. (biologists.org)
  • Although Rho-family small G proteins have been implicated in actin organization in many cell types, it is not fully elucidated whether Rho mediates the organization of actin fibers by hypertrophic stimuli in cardiac myocytes. (ahajournals.org)
  • Activation
  • In an effort to identify novel therapeutic targets for autoimmunity and transplant rejection, we developed and performed a large-scale retroviral-based functional screen to select for proteins that inhibit antigen receptor-mediated activation of lymphocytes. (nih.gov)
  • However, fibrinogen binding caused Csk to dissociate from alphaIIbbeta3, concomitant with dephosphorylation of Src Tyr-529 and phosphorylation of Src activation loop Tyr-418. (nih.gov)
  • Syk-deficient platelets exhibited Src activation upon adhesion to fibrinogen, but no spreading or phosphorylation of Vav1, Vav3, and SLP-76. (nih.gov)
  • These studies establish that platelet spreading on fibrinogen requires sequential activation of Src and Syk in proximity to alphaIIbbeta3, thus providing a paradigm for initiation of integrin signaling to the actin cytoskeleton. (nih.gov)
  • The results presented so far are consistent with the idea that Src activation may occur in localized regions of the platelet where αIIbβ3 first comes in contact with fibrinogen. (nih.gov)
  • Thus, Src activation takes place in association with αIIbβ3 and at the periphery of spreading platelets. (nih.gov)
  • Hyper-activation of pp60Src limits nitric oxide signaling by increasing asymmetric dimethylarginine levels during acute lung injury. (arizona.edu)
  • The mechanism of IL-6 -induced AR activation is mediated through enhancing AR- SRC-1 interaction and inhibiting AR- SMRT interaction. (antibodies-online.com)
  • Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. (abcam.com)
  • phosphotyrosine
  • Using antibodies to phosphotyrosine (aPTyr) in Western blots, it has been demonstrated that tyrosine-specific protein phosphorylati on occurs when human or rabbit neutrophils are stimulated with a variety of agonists, including platelet-activating factor (PAF), human cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor (TNF), the chemotactic peptide fMet-Leu-Phe, phorbol esters (PMA) and the calcium ionophore A23187 (9-17). (springer.com)
  • mDab1 can also form complexes with cellular phosphotyrosyl proteins through a domain that is related to the phosphotyrosine binding (PTB) domains of the Shc family of adaptor proteins. (embopress.org)
  • The mDab1 PTB domain binds to phosphotyrosine‐containing proteins of 200, 120 and 40 kDa from extracts of embryonic mouse heads. (embopress.org)
  • Numerous developmental processes are regulated by signaling cascades that alter protein phosphotyrosine levels. (embopress.org)
  • adhesion
  • Platelet adhesion to fibrinogen caused a rapid increase in alphaIIbbeta3-associated Src activity, and active Src localized to filopodia and cell edges.Csk, which negatively regulates Src by phosphorylating Tyr-529, was also constitutively associated with alphaIIbbeta3.In contrast to the behavior of Src and Csk, Syk was associated with alphaIIbbeta3 only after fibrinogen binding. (nih.gov)
  • Platelet adhesion to fibrinogen caused a rapid increase in alphaIIbbeta3-associated Src activity, and active Src localized to filopodia and cell edges. (nih.gov)
  • These specific defects point to the existence of adhesion‐stimulated, Src‐ and Fyn‐dependent, regulatory processes required for neurite extension. (embopress.org)
  • The belt can further condense, giving rise to a ∼4-μm-wide actin domain flanked by outer and inner plaque domains containing integrins and focal adhesion proteins. (biologists.org)
  • amino acids
  • Indicated in blue is the myristoylation signal from c-Src (MS), containing the NH2-terminal 20 amino acids. (nih.gov)
  • lysates
  • A 1:1000 dilution of this lot detected phospho-c-Src in RIPA lysates from COS cells transfected with active Src (Cat # 21-115) (Figure A). (merckmillipore.com)
  • A previous lot detected endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) (Figure B). (merckmillipore.com)
  • intrinsic
  • The PDBFlex database explores the intrinsic flexibility of protein structures by analyzing structural variations of the same protein across the archive. (rcsb.org)