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  • Mutation
  • Patients with the CYP2E1 Dra cd genotype were less likely to have a K-ras mutation: of 55 patients with the wild-type CYP2E1 genotype (dd), 23 had K-ras mutations, whereas none of the 7 individuals with cd genotype had a K-ras mutation (P=0.04). (openrepository.com)
  • In this prospective-retrospective analysis, we assessed the efficacy and safety of panitumumab plus oxaliplatin, fluorouracil, and leucovorin (FOLFOX4) as compared with FOLFOX4 alone, according to RAS (KRAS or NRAS) or BRAF mutation status. (nih.gov)
  • Our data support potential interactions between the VDR, RAS-MAPK and PI3K-AKT pathways, and possible influence by KRAS or PIK3CA mutation on therapy or chemoprevention targeting VDR. (nih.gov)
  • Kras
  • Our studies reveal that oncogenic Kras induces SOX9 mRNA and protein expression as well as phosphorylated SOX9 expression in human pancreatic ductal progenitor cells (HPNE) and pancreatic ductal cells (HPDE). (nih.gov)
  • Previous efforts to develop drugs that directly inhibit the activity of mutant KRAS, the most commonly mutated human oncogene, have not been successful. (nih.gov)
  • a) Anti-Flag IPs were performed with KRAS WT BT-20 and KRAS mutant MDA-MB-231 breast cancer cell lines stably transduced with empty vector (EV), N-terminally Flag-tagged STK33 (Flag-STK33), or C-terminally Flag-tagged STK33 (STK33-Flag), and the resulting protein complexes were separated by PAGE and stained with Coomassie. (nih.gov)
  • Adaptor Proteins
  • The core inhibitory kinase module is composed of two groups of kinases, the mammalian STE20-like protein kinase 1 (MST1) and MST2, and the large tumour suppressor 1 (LATS1) and LATS2, in combination with their activating adaptor proteins, salvador family WW domain-containing protein 1 (SAV1), MOB kinase activator 1A (MOB1A) and MOB1B. (nih.gov)
  • pharmacological
  • therefore, it is reasonable to speculate that a pharmacological approach that curtails Ras activity may represent a sensible approach to inhibit melanoma growth. (curehunter.com)
  • immunohistochemical
  • A-C ) β-Galactosidase staining at pH 6, ( D-F ) p53 immunohistochemical staining, ( G-I ) p21 immunohistochemical staining, and ( J-L ) MCM2 immunohistochemical staining, in sections of frozen (β-galactosidase) or formalin-fixed paraffin-embedded pancreatic tissue. (nih.gov)
  • regulation
  • In this Progress article, we summarize the current understanding of the biological functions of YAP and TAZ, and how the regulation of these two proteins can be disrupted in cancer. (nih.gov)
  • Regulation of yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) activity goes well beyond the core kinases in the Hippo pathway. (nih.gov)
  • disassembly
  • Taken together, these data indicate that insulin, EGF, and PDGF all cause disassembly of Sos proteins from signaling complexes during p21ras desensitization, but at least two mechanisms are involved. (umassmed.edu)
  • PDGF action triggers both mechanisms of Grb2 disassembly, which probably operate in concert with GAP to attenuate p21ras signaling. (umassmed.edu)
  • Disassembly of Son-of-sevenless proteins from Grb2 during p21ras desen" by Andrew D. Cherniack, Jes K. Klarlund et al. (umassmed.edu)
  • The disassembly of Grb2 from Sos proteins slightly precedes the time course of p21ras deactivation in response to insulin. (umassmed.edu)
  • inhibition
  • Sulforaphane, quercetin and catechins complement each other in elimination of advanced pancreatic cancer by miR-let-7 induction and K-ras inhibition. (ox.ac.uk)
  • Following treatment with bioactive agents, the expression of miR-let7-a was specifically induced in cancer cells but not in normal cells and it was associated with K-ras inhibition. (ox.ac.uk)
  • These data demonstrate that sulforaphane, quercetin and GTC complement each other in inhibition of PDA progression by induction of miR-let7-a and inhibition of K-ras. (ox.ac.uk)
  • mutant
  • This progression is consistent with failed growth arrest and/or senescence of premalignant lesions, since a mutant of p53, p53(R172P), which can still induce p21 and cell cycle arrest, is resistant to PDAC formation. (nih.gov)
  • Nucleotide
  • Insulin receptor signaling acutely stimulates GTP loading of p21ras, apparently by mobilizing complexes of Grb2 and the guanine nucleotide exchangers Son-of-sevenless (Sos) 1 and 2 to associate with tyrosine-phosphorylated proteins in the plasma membrane. (umassmed.edu)
  • Cells
  • Lysates of such desensitized cells were quantitatively immunoprecipitated with an antiserum recognizing both Sos1 and Sos2 proteins or a specific anti-Sos2 antiserum. (umassmed.edu)
  • These data are consistent with the hypothesis that the dissociation of Grb2 from Sos proteins caused by insulin in 3T3-L1 cells mediates p21ras deactivation and desensitization. (umassmed.edu)
  • Here, using a mass spectrometry-based screen for STK33 protein interaction partners, we report that the HSP90/CDC37 chaperone complex binds to and stabilizes STK33 in human cancer cells. (nih.gov)
  • d) Protein expression of HSP90A, HSP90B, STK33, and cleaved PARP in lung (NCI-H520, A549) and colon (Caco-2, HCT-116) carcinoma cells transduced with a nontargeting control shRNA or a shRNA-targeting HSP90A or HSP90B. (nih.gov)
  • complexes
  • Furthermore, elevated amounts of Grb2/Shc complexes persisted throughout p21ras desensitization by insulin. (umassmed.edu)
  • However, immunoblotting of anti-Son-of-sevenless (Sos) 1 and 2 immunoprecipitates with anti-Grb2 antisera revealed that p21ras desensitization in response to insulin and PDGF, but not EGF, is associated with a marked decrease in cellular complexes containing Sos and Grb2 proteins. (umassmed.edu)
  • insulin
  • Immunoblot analysis of these precipitates revealed that insulin causes a marked hyperphosphorylation of Sos1 and a 50% decrease in Grb2 associated with Sos proteins under these conditions. (umassmed.edu)
  • Similarly, anti-Grb2 immunoprecipitates of such lysates revealed the presence of decreased Sos1 protein due to insulin action. (umassmed.edu)
  • major
  • The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. (cancerindex.org)