• c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I. (fhcrc.org)
  • Grandori, Carla and Gomez-Roman, Natividad and Felton-Edkins, Zoe A and Ngouenet, Celine and Galloway, Denise A and Eisenman, Robert N and White, Robert J (2005) c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I. Nature cell biology, 7 (3). (fhcrc.org)
  • c-Myc coordinates cell growth and division through a transcriptional programme that involves both RNA polymerase (Pol) II- and Pol III-transcribed genes. (fhcrc.org)
  • Here, we demonstrate that human c-Myc also directly enhances Pol I transcription of ribosomal RNA (rRNA) genes. (fhcrc.org)
  • EPIC1 knockdown reduces the occupancy of MYC to its target genes (e.g. (prinsesmaximacentrum.nl)
  • In the past 5 years, many high-throughput screens based on microarray gene expression profiling, serial analysis of gene expression (SAGE), chromatin immunoprecipitation (ChIP) followed by genomic array analysis, and Myc-methylase chimeric proteins have generated a wealth of information regarding Myc responsive and target genes. (elsevierpure.com)
  • From these studies, the c-Myc target gene network is estimated to comprise about 15% of all genes from flies to humans. (elsevierpure.com)
  • Both genomic and functional analyses of c-Myc targets suggest that while c-Myc behaves as a global regulator of transcription, groups of genes involved in cell cycle regulation, metabolism, ribosome biogenesis, protein synthesis, and mitochondrial function are over-represented in the c-Myc target gene network. (elsevierpure.com)
  • c-Myc also consistently represses genes involved in cell growth arrest and cell adhesion. (elsevierpure.com)
  • The overexpression of c-Myc predisposes cells to apoptosis under nutrient or growth factor deprivation conditions, although the critical sets of genes involved remain elusive. (elsevierpure.com)
  • Despite tremendous advances, the downstream target genes that distinguish between physiologic and tumorigenic functions of c-Myc remain to be delineated. (elsevierpure.com)
  • Uncovering the genes regulated by c-Myc is an essential step for understanding these processes. (huji.ac.il)
  • Promoter methylation of p16 INK4A , c-myc and hMSH2 genes was assayed by methylation-specific PCR (MSP) and sequencing (mapping). (wjgnet.com)
  • In particular, c-MYC was identified as a candidate upstream regulator of OGT target genes and OGT inhibition elicited a dose-dependent decrease in the levels of c-MYC protein but not c-MYC mRNA in cell lines. (ox.ac.uk)
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) were tested 20 times on one plate, respectively. (biomatik.com)
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) were tested on 3 different plates, 8 replicates in each plate. (biomatik.com)
  • This assay has high sensitivity and excellent specificity for detection of V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC). (biomatik.com)
  • No significant cross-reactivity or interference between V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) and analogues was observed. (biomatik.com)
  • This Recombinant Human MYCN protein is an essential resource for cancer researchers. (cusabio.com)
  • Equipped with an N-terminal 6xHis-tag, our Recombinant Human MYCN protein facilitates effortless purification and detection. (cusabio.com)
  • Choose between liquid and lyophilized powder forms to best suit your experimental requirements and elevate your research with our Recombinant Human MYCN protein. (cusabio.com)
  • Review: In vitro glutathione S-transferase (GST) pull-down assay with recombinant GST-CCNT1, GST-CDK9, and GST-EAF1 together with His-tagged MYCN proteins demonstrated that MYCN directly binds to EAF1 with the highest affinity. (cusabio.com)
  • N-myc proto-oncogene protein also known as N-Myc or basic helix-loop-helix protein 37 (bHLHe37), is a protein that in humans is encoded by the MYCN gene. (wikipedia.org)
  • The MYCN gene is a member of the MYC family of transcription factors and encodes a protein with a basic helix-loop-helix (bHLH) domain. (wikipedia.org)
  • The MYCN gene has an antisense RNA, N-cym or MYCNOS, transcribed from the opposite strand which can be translated to form a protein product. (wikipedia.org)
  • This NCYM protein inhibits GSK3b and thus prevents MYCN degradation. (wikipedia.org)
  • A basic helix-loop-helix leucine zipper (bHLHZ) transcription factor and proto-oncogene protein that functions in cell growth and proliferation. (bvsalud.org)
  • Gene-regulatory properties of Myc helix-loop-helix/leucine zipper mutants: Max-dependent DNA binding and transcriptional activation in yeast correlates with transforming capacity. (ox.ac.uk)
  • Max is a basic helix-loop-helix/leucine zipper (bHLH/LZ) protein that forms sequence-specific DNA-binding complexes with the c-Myc oncoprotein (Myc). (ox.ac.uk)
  • p53 Loss in MYC-Driven Neuroblastoma Leads to Metabolic Adaptations Supporting Radioresistance. (icr.ac.uk)
  • La amplificación o sobreexpresión de N-Myc ocurre en más del 20 por ciento de los tumores y se asocia a mal pronóstico en casos de NEUROBLASTOMA, RABDOMIOSARCOMA ALVEOLAR, CARCINOMA PULMONAR DE CÉLULAS PEQUEÑAS y cáncer de próstata neuroendocrino. (bvsalud.org)
  • The role of histone demethylase KDM4B in Myc signaling in neuroblastoma. (umassmed.edu)
  • Amplification and overexpression of N-Myc can lead to tumorigenesis. (wikipedia.org)
  • Mdm2 Phosphorylation Regulates Its Stability and Has Contrasting Effects on Oncogene and Radiation-Induced Tumorigenesis. (umassmed.edu)
  • We propose that stimulation of rRNA synthesis by c-Myc is a key pathway driving cell growth and tumorigenesis. (fhcrc.org)
  • MYC depletion abolishes EPIC1's regulation of MYC target and luminal breast cancer tumorigenesis in vitro and in vivo. (prinsesmaximacentrum.nl)
  • AMBRA1 is an intrinsically disordered protein, working as a scaffold molecule to coordinate, by protein-protein interaction, many cellular processes, including autophagy, mitophagy, apoptosis and cell cycle progression. (biomedcentral.com)
  • The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. (arigobio.com)
  • Mechanistically, EPIC1 promotes cell-cycle progression by interacting with MYC through EPIC1's 129-283 nt region. (prinsesmaximacentrum.nl)
  • The purpose of this study is to investigate the effect of metformin on c-myc expression and PCa progression. (nyu.edu)
  • Our results demonstrated that (i) in Hi-Myc mice that display murine prostate neoplasia and highly resemble the progression of human prostate tumors, metformin attenuated the development of prostate intraepithelial neoplasia (PIN, the precancerous lesion of prostate) and PCa lesions. (nyu.edu)
  • We first elucidated their essential function in ESCC progression and further explored their preliminary mechanism by identifying target proteins and involving signal pathways. (bvsalud.org)
  • NFAT2, as an important transcriptional promoter, regulates expression of TNF-α, myc proto-oncogene protein (c-myc), cyclooxygenase-2 (COX-2), Fas ligand (FasL) and also generates crosstalks with ERK/MAPK pathway and AKT/GSK3b signaling, which achieves its control of the cell fate [7-12]. (researchsquare.com)
  • In addition, we have used yeast to investigate the role of the Myc helix-loop-helix (HLH) and leucine zipper (LZ) motifs in mediating Max-dependent DNA-binding and transcriptional activation in vivo using HLH/LZ mutants generated by site-directed mutagenesis. (ox.ac.uk)
  • Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human Burkitt's lymphoma tissue stained with ARG66925 anti-c-Myc antibody [SQab22253]. (arigobio.com)
  • Immunofluorescence analysis of HeLa cells treated with Forskolin 40nM 30', using Myc (Phospho-Ser62) Antibody. (omnimabs.com)
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Ser62) Antibody. (omnimabs.com)
  • The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from 293 cells treated with Forskolin 40nM 30', using Myc (Phospho-Ser62) Antibody. (omnimabs.com)
  • The c-myc protein is localized in the nucleus and can physically associate with RB protein in vitro, hence c-myc may functionally antagonize RB function. (wdv.com)
  • ii) Metformin reduced c-myc protein levels in vivo and in vitro. (nyu.edu)
  • CDK9 has important functions in cell cycle regulation, including the modulation of two therapeutic targets in cancer: myeloid leukemia cell differentiation protein ("MCL1") and the MYC proto-oncogene protein ("MYC") which regulates cell proliferation and growth. (bentoncourier.com)
  • Excess N-Myc is associated with a variety of tumors, most notably neuroblastomas where patients with amplification of the N-Myc gene tend to have poor outcomes. (wikipedia.org)
  • Here we show that Tmp is specifically highly expressed in mammary tumors and T-cell lymphomas which develop in c-myc transgenic mice, suggesting that Tmp expression is a general characteristic of c-Myc-induced tumors. (huji.ac.il)
  • The overexpression of miRNA‑375 significantly increased caspase‑3 and caspase‑9 activities, induced B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein, tumor protein 53 and cyclin‑dependent kinase inhibitor 1 protein expression and suppressed cyclin D1 and survivin protein expression in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
  • The overexpression of miRNA‑375 significantly suppressed the levels of protein expression of ubiquitin‑protein ligase E3A (UBE3A) and Insulin‑like growth factor‑1 receptor (IGF‑1R) in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
  • O-GlcNAc transferase integrates metabolic pathways to regulate the stability of c-MYC in human prostate cancer cells. (ox.ac.uk)
  • Here, we report that the HBP is activated in prostate cancer cells and that OGT is a central regulator of c-Myc stability in this setting. (ox.ac.uk)
  • Supporting this relationship, expression of c-MYC and OGT was tightly correlated in human prostate cancer samples (n = 1306). (ox.ac.uk)
  • Our findings identify HBP as a modulator of prostate cancer growth and c-MYC as a key target of OGT function in prostate cancer cells. (ox.ac.uk)
  • Myc-dependent purine biosynthesis affects nucleolar stress and therapy response in prostate cancer. (lu.se)
  • The Myc protein and proteins that participate in mitosis represent attractive targets for cancer therapy. (nih.gov)
  • TPD drugs are an emerging modality that selectively targets disease-causing proteins by leveraging intracellular protein degradation mechanisms, such as the ubiquitin-proteasome system and autophagy. (pharmaceutical-technology.com)
  • It has been shown that the antisense RNA encodes for a protein, named NCYM, that has originated de novo and is specific to human and chimpanzee. (wikipedia.org)
  • Synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc. (enzolifesciences.com)
  • Recognizes epitope sequence EQKLISEEDL, located in aa 410-419 of human c-myc protein. (enzolifesciences.com)
  • Synthetic peptide derived from Human c-Myc. (arigobio.com)
  • The antiserum was produced against synthesized peptide derived from human Myc around the phosphorylation site of Ser62. (omnimabs.com)
  • Our data also show that c-Myc binds to specific consensus elements located in human rDNA and associates with the Pol I-specific factor SL1. (fhcrc.org)
  • To explore the effect of DNA methyltransferase, demethylase and methyl-CpG binding protein MeCP2 on the expressions and methylation of hMSH2 and proto-oncogene in human gastric cancer. (wjgnet.com)
  • The up-regulation of proto-oncogene may be the consequence of epigenetic control of gene expression by demethylase, and mbd2 is involved in the regulation of hMSH2 expression in human gastric cancer. (wjgnet.com)
  • Immunohistochemical analysis of human specimens (n = 1987) established that OGT is upregulated at the protein level and that its expression correlates with high Gleason score, pT and pN stages, and biochemical recurrence. (ox.ac.uk)
  • The amplification of c-MYC oncogene plays a key role in early prostate epithelia cell transformation and PCa growth. (nyu.edu)
  • In addition, numerous important cellular proteins, such as B-cell lymphoma-2 homologous antagonist/killer, Myc proto-oncogene protein, cyclin-dependent kinase inhibitor 1B, DNA replication licensing factor MCM-7, retinoblastoma 1 and Annexin A1, are degenerated through the UBE3A-mediated ubiquitin proteasome pathway ( 7 ). (spandidos-publications.com)
  • iv) Reduced PIN formation by metformin was associated with reduced levels of androgen receptor and proliferation marker Ki-67 in Hi-Myc mouse prostate glands. (nyu.edu)
  • Cells differentiating into neuroectoderm undergo apoptosis in the absence of functional retinoblastoma family proteins. (mcmaster.ca)
  • N-Myc is also stabilized by aurora A which protects it from degradation. (wikipedia.org)
  • In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. (arigobio.com)
  • For more than a decade, numerous studies have suggested that the c-Myc oncogenic protein is likely to broadly influence the composition of the transcriptome. (elsevierpure.com)
  • Oncogenic PKA signaling increases c-MYC protein expression through multiple targetable mechanisms. (ucsf.edu)
  • We have isolated the Tmp promoter region and identified a putative c-Myc binding element, CACGTG, located in the first intron of the gene. (huji.ac.il)
  • We show here that constructs containing the Tmp regulatory region fused to a reporter gene are activated by c-Myc through this CACGTG element and that the c-Myc-Max protein complex can bind to this element. (huji.ac.il)
  • Using Saccharomyces cerevisiae, we have shown that the Max bHLH/LZ domain enables Myc to activate transcription through CACGTG and CACATG sequences in vivo, and that the number and context of such sites determines the level of activation. (ox.ac.uk)
  • rRNA synthesis and accumulation occurs rapidly following activation of a conditional MYC-ER allele (coding for a Myc-oestrogen-receptor fusion protein), is resistant to inhibition of Pol II transcription and is markedly reduced by c-MYC RNA interference. (fhcrc.org)
  • At last, the mRNA and protein expression of NFAT2, Egr2, FasL, COX-2 and c-myc in carcinoma and adjacent tissues was investigated. (researchsquare.com)
  • Results from zebrafish embryos injected with murine Stat3 mRNA and stat3 morpholino suggest that Ambra1b could indirectly regulate this protein through CUL4-DDB1 interaction. (biomedcentral.com)
  • Targeted protein degrader (TPD) drugs have seen a greater than 2,000% increase in the total value of venture financing deals, rising from $33 million in 2017 to $707 million in 2022, according to GlobalData's Pharma Intelligence Center Deals Database. (pharmaceutical-technology.com)
  • Here we report that a circumscribed exposure to the aurora kinase inhibitor, VX-680, selectively kills cells that overexpress Myc. (nih.gov)
  • The hexosamine biosynthetic pathway (HBP) senses metabolic status and produces an essential substrate for O-linked β-N-acetylglucosamine transferase (OGT), which glycosylates and thereby modulates the function of its target proteins. (ox.ac.uk)
  • The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. (arigobio.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • As activation of c-myc expression promotes cell proliferation and blocks differentiation, it may positively regulate cell growth. (wdv.com)
  • In addition, TMp expression is induced upon serum stimulation of fibroblasts as shown in a time course closely correlated with c-myc expression. (huji.ac.il)
  • N-myc proto-oncogene protein, also known as Class E basic helix-loop-helix protein 37 (bHLHe37), plays a critical role in cancer research. (cusabio.com)
  • Furthermore, by using combined immunofluorescence and rRNA-FISH, we have detected endogenous c-Myc in nucleoli at sites of active ribosomal DNA (rDNA) transcription. (fhcrc.org)
  • Moreover, an inducible form of c-Myc, the MycER fusion protein, can activate the endogenous Tmp gene. (huji.ac.il)
  • and effective against mouse models for B-cell and T-cell lymphomas initiated by transgenes of MYC. (nih.gov)
  • Replacement of wild-type RB can suppress the tumorigenicity of some of these cells, suggesting that the RB protein (Rb) may negatively regulate cell growth. (wdv.com)
  • Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). (arigobio.com)
  • The presence of c-Myc at specific sites on rDNA coincides with the recruitment of SL1 to the rDNA promoter and with increased histone acetylation. (fhcrc.org)
  • N-Myc and MYCNOS are co-regulated both in normal development and in tumor cells, so it is possible that the two transcripts are functionally related. (wikipedia.org)
  • UBE3A is associated with cervical cancer and may combine with the E6 proto-oncogene encoded by HPV16 within cervical cancer cells to form the E6/E6-AP protein complex through the ubiquitin proteasome pathway ( 4 ). (spandidos-publications.com)