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  • transcription
  • We also show that HDAC1 and Mdm2 function co-operatively to reduce AR-mediated transcription that is attenuated by the HAT activity of the AR co-activator Tip60, suggesting interplay between acetylation status and receptor ubiquitylation in AR regulation. (ox.ac.uk)
  • Mutation
  • Immunohistochemistry and high resolution melting were used to determine p53 protein expression and TP53 mutation status respectively in normal gastric mucosa, IM without concurrent GC (IM-GC), IM with concurrent GC (IM+GC) and GC. (edu.au)
  • MeSH
  • Ubiquitin-Protein Ligases" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
  • Proto-Oncogene Proteins c-bcl-2" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
  • Binding
  • The interference of binding to p53 prevents the interaction of MDM2 and its regulation of the transcriptional activity of p53 in vivo. (genetex.com)
  • Protein-bead complexes were washed and analyzed for AR binding by Western blot analysis ( Upper ) using anti-AR antibodies. (nih.gov)
  • Regulation of the AR is achieved by alternate binding of either histone acetyltransferase (HAT)-containing co-activator proteins, or histone deacetylase 1 (HDAC1). (ox.ac.uk)
  • mechanism
  • Factors that control AR stability may also constitute an important regulatory mechanism, a notion that has been confirmed with the finding that the AR is a direct target for Mdm2-mediated ubiquitylation and proteolysis. (ox.ac.uk)
  • Furthermore, we demonstrate that Mdm2-mediated modification of AR and HDAC1 catalyses protein destabilization and attenuates AR sactivity, suggesting that ubiquitylation of the AR and HDAC1 may constitute an additional mechanism for regulating AR function. (ox.ac.uk)
  • Mice
  • Li S, Shu B, Zhang Y, Li J, Guo J, Wang Y, Ren F, Xiao G, Chang Z, Chen D. Carboxyl terminus of Hsp70-interacting protein regulation of osteoclast formation in mice through promotion of tumor necrosis factor receptor-associated factor 6 protein degradation. (rush.edu)
  • marker proteins
  • The resultant nuclear fraction is void of cytosolic and endoplasmic reticulum contamination as evidenced by lack of the marker proteins GAPDH and calnexin, respectively. (nih.gov)
  • Furthermore, BMH-21 induced the expression of nucleolar stress marker proteins (nucleolin, nucleophosmin and fibrillarin) and promoted the nuclear export of these proteins. (ingentaconnect.com)
  • ligase
  • betaArrestin2 serves as an adapter, bringing into close proximity the Mdm2 E3 ligase and AR, thereby promoting AR ubiquitylation and degradation. (nih.gov)
  • Ramirez PW, DePaula-Silva AB, Szaniawski M, Barker E, Bosque A, Planelles V. HIV-1 Vpu utilizes both cullin-RING ligase (CRL) dependent and independent mechanisms to downmodulate host proteins. (rush.edu)
  • TP53
  • The RAS proteins in turn signal through six alternative effector families to affect many downstream targets including TP53, RB1, and MTOR. (cancer.gov)
  • membrane
  • The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. (rush.edu)
  • Variation
  • Zhuo X, Ye H, Li Q, Xiang Z, Zhang X. Is MDM2 SNP309 Variation a Risk Factor for Head and Neck Carcinoma? (sinobiological.com)
  • We report a dual strategy in ∼70,000 women to identify common and low-frequency protein-coding variation associated with age at natural menopause (ANM). (cancerindex.org)
  • invasion
  • Evidence from in vitro studies indicates that the widely expressed liprin-α1 protein regulates the migration and invasion of human breast cancer cells. (cancerindex.org)
  • PMID
  • Dysregulation of MDM2 has been identified in several cancer types ( PMID: 23303139 ) and amplification is common in liposarcoma ( PMID: 30237864 ). (jax.org)
  • regulates
  • In the cell liprin-α1 forms a complex with liprin-β1, ERC1/ELKS and LL5 proteins, which localizes at the front of migrating cells and positively regulates lamellipodia stability, and integrin-mediated focal adhesions. (cancerindex.org)
  • Enzymes
  • A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. (rush.edu)
  • LNCaP
  • Purified GST, GST-βArr1, or GST-βArr2 proteins were incubated with LNCaP cell lysate in the presence of glutathione-Sepharose beads. (nih.gov)
  • Using chromatin immunoprecipitation (ChIP) and re-ChIP analyses, we show that Mdm2 associates with AR and HDAC1 at the active androgen-responsive PSA promoter in LNCaP prostate cancer cells. (ox.ac.uk)
  • indicates
  • In all, our data indicates a novel role for Mdm2 in regulating components of the AR transcriptosome. (ox.ac.uk)
  • levels
  • Here we show that the wild-type p53-induced phosphatase 1 (Wip1), or PPM1D, downregulates p53 protein levels by stabilizing Mdm2 and facilitating its access to p53. (umassmed.edu)
  • disease
  • Khasnavis S, Pahan K. Cinnamon treatment upregulates neuroprotective proteins Parkin and DJ-1 and protects dopaminergic neurons in a mouse model of Parkinson's disease. (rush.edu)
  • abnormal
  • These ASF proteins may be used to detect abnormal lesions in the lung without collecting invasive biopsy specimens. (labome.org)