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  • ERK1
  • IQGAP1 regulates ERK1/2 and AKT signalling in the heart and sustains functional remodelling upon pressure overload. (biomedsearch.com)
  • Here, we investigated the role of IQ motif-containing GTPase-activating protein 1 (IQGAP1), a scaffold for the ERK1/2 cascade, in heart function and remodelling in response to pressure overload. (biomedsearch.com)
  • Pull-down experiments indicated that IQGAP1 is able to bind the three components of the ERK cascade, namely c-Raf, MEK1/2, and ERK1/2, as well as AKT in the heart. (biomedsearch.com)
  • Effects of CTT on stimulation of MEK-ERK1/2 and Akt pathways induced by Grp94-IgG complexes. (nih.gov)
  • After 18 hrs incubation, cells were lysed with the Laemmli buffer added with 7 mM β-mercaptoethanol, and whole lysates analysed in Western blotting for total and phosphorylated forms of ERK1/2 (A) and Akt proteins (B). Membranes were then probed for β actin for normalization of protein content. (nih.gov)
  • nerve growth
  • To investigate the role of Akt in nerve growth factor (NGF)-induced neuronal differentiation, PC12 cells ectopically expressing wild-type or dominant-inhibitory forms of Akt were analyzed. (biomedsearch.com)
  • The term nerve growth factor usually refers to the 2.5S, 26-kDa beta subunit of the protein, the only component of the 7S NGF complex that is biologically active (i.e. acting as signaling molecules). (wikipedia.org)
  • eNOS
  • HET0016 treatment decreased oxidative stress and increased the phosphorylated state of key proteins that regulate endothelial function, including eNOS, AKT, and AMPK. (curehunter.com)
  • Activation
  • Scaffold proteins are key elements in coordinating signalling molecules for their appropriate spatiotemporal activation. (biomedsearch.com)
  • Induction of BSMC proliferation and migration by IPDI-BEAS-2B-CM and IPDI-HBEC-CM was associated with increased focal adhesion kinase (FAK), Src, extracellular signal-regulated kinase (ERK)1/2 and AKT activation. (biomedsearch.com)
  • Direct binding and activation of STAT transcription factors by the herpesvirus saimiri protein Tip. (wikipedia.org)
  • caspase
  • These protective effects of BpV(HOpic) are associated with increased phospho-Akt and the repression of caspase-3 activity. (biomedsearch.com)
  • Intact form of caspase-3 was maximal at proestrus and was reduced only at estrus.Likewise, presence of a specific cleaved caspase-3 fragment was observed only at estrus and IHC revealed that cleaved caspase-3 signal was found in luminal epithelial cells.Expression of phospho-Akt (the activated form of Akt) was present at metestrus, diestrus, and proestrus but decreased significantly at estrus. (nih.gov)
  • neuronal
  • In summary, our results indicate that Akt overrides the growth-arresting effect of NGF and thereby, negatively regulates neuronal differentiation. (biomedsearch.com)
  • cells
  • NGF-induced neurite outgrowth was greatly accelerated in cells expressing dominant-inhibitory Akt, compared to parental PC12 cells, but was almost completely blocked in cells expressing wild-type Akt. (biomedsearch.com)
  • Cells expressing wild-type Akt were not susceptible to the growth-arresting effect of NGF, whereas parental PC12 cells and notably cells expressing mutant Akt were so affected. (biomedsearch.com)
  • Accompanying this, the expressions of CDKs and p21(WAF1) were down- and up-regulated, respectively, in both parental PC12 cells and cells expressing mutant Akt. (biomedsearch.com)
  • When treated with some growth arrest-inducing agents such as sodium nitroprusside, forskolin and butyrolactone I, cells expressing wild-type Akt regained their responsiveness to the effects of NGF on differentiation. (biomedsearch.com)
  • The expression of PTEN mRNA and protein in OV2008 cells and C13K cells were semi-quantitatively detected by using RT-PCR and Western blotting. (biomedsearch.com)
  • The expression of PTEN mRNA was monitored by RT-PCR and the expression of PTEN, Akt, p-Akt protein were analyzed by Western blotting in PTEN-transfected and non-transfected C13K cells. (biomedsearch.com)
  • The expression of PTEN mRNA and protein in OV2008 cells were significantly higher than those in C13K cells. (biomedsearch.com)
  • IHC revealed that Akt expression was high in both stromal and epithelial cells at estrus. (nih.gov)