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ProteomeGelsChromatography, High Pressure LiquidMass SpectrometryChromatography, AffinityProteomicsChromatography, LiquidElectrophoresis, Gel, Two-DimensionalChromatography, GelChromatographyChromatography, Ion ExchangeChromatography, GasTandem Mass SpectrometryChromatography, Thin LayerSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationMolecular Sequence DataSpectrometry, Mass, Electrospray IonizationAmino Acid SequenceMolecular WeightElectrophoresis, Polyacrylamide GelChromatography, DEAE-CelluloseKineticsGas Chromatography-Mass SpectrometryChromatography, AgaroseChemical FractionationBody Mass IndexChromatography, Reverse-PhaseIsoelectric FocusingBacterial ProteinsProteinsPeptidesIsotope LabelingHydrogen-Ion ConcentrationAmino AcidsProtein Array AnalysisSubstrate SpecificityDatabases, ProteinTwo-Dimensional Difference Gel ElectrophoresisBlood ProteinsEscherichia coliElectrophoresis, Gel, Pulsed-FieldBase SequencePeptide MappingChromatography, PaperElectrophoresis, Agar GelTrypsinSepharoseProtein BindingPeptide FragmentsCattleComputational BiologyLiverReproducibility of ResultsSequence Analysis, ProteinIsoelectric PointTemperaturePlant ProteinsRecombinant ProteinsSequence Homology, Amino AcidSpectrophotometry, UltravioletRabbitsBlotting, WesternCell LineCloning, MolecularMembrane ProteinsChemistryReference StandardsChemical PhenomenaSpecies SpecificityTime FactorsProtein Processing, Post-TranslationalCarbohydratesGene Expression ProfilingHydrolysisBinding SitesMagnetic Resonance SpectroscopyCarbohydrate SequenceIndicators and ReagentsMethodsPolysaccharidesDrug StabilitySensitivity and SpecificityCells, CulturedBiological MarkersElectrophoresisSolubilityHot TemperatureSaccharomyces cerevisiaeTranscriptomeSwineCell MembraneCarrier ProteinsGlycoproteinsOxidation-ReductionOligosaccharidesCountercurrent DistributionModels, BiologicalCell FractionationMutationRNA, Messenger
Tandem mass spectroElectrophoresisProteomicsProteinsPeptidesQuantificationQuantitativeElectrosprayProteomicSeparationLipidomeCharacterizationHPLCAffinitySpectrometricPost-TranslationalHigh throughputMethodsSpectrometry analysisAntibodiesAnalysisLiquidAnalyzeInteractionUrineCharacterizeDefiningSpectraDefined as the protein comGenomeTissuesSample PreparationResponsesBioinformaticsSpectrometerHumanResearchMiceJournalDetection
Tandem mass spectro6
Electrophoresis11
  • Serum or Plasma Sample Preparation for Two-Dimensional Gel Electrophoresis - Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari and Craig D. Shriver 7. (nhbs.com)
  • Large-Format Two-Dimensional Polyacrylamide Gel Electrophoresis - Henry Brzeski, Stephen Russell, Tony G. Sullivan, Richard Somiari & Craig Shriver 14. (nhbs.com)
  • Techniques used in human sperm proteome mapping: There are several initial reports using MS proteomics technology to identify a limited number of proteins from the human sperm using twodimensional gel electrophoresis (2-DE) coupled to MALDI-TOF-MS analysis (16 - 23). (jri.ir)
  • Firstly, proteins were isolated with a detergent-containing buffer that allowed extraction of various groups of storage proteins (glutenins, gliadins, globulins, and albumins), secondly, the proteome was profiled by the two-dimensional gel electrophoresis. (cas.cz)
  • The WFZ-Fungene joins the equipment and expertise for the production of DNA- and protein microarrays, for two dimensional gel electrophoresis as well as for gel free multi dimensional chromatographic proteome analysis. (marine-biotechnologie.de)
  • MudPIT) and Fluorescence two-dimensional differential in-gel electrophoresis (2D-DIGE) to identify potential biomarkers of PTL and SPTB. (elsevierpure.com)
  • Using a two-dimensional electrophoresis matrix-assisted laser desorption/ionisation-time of flight mass spectrometry investigation coupled to an immunohistochemical approach, we have assessed the expression of αB-crystallin in IDCAs, as well as, in other types of breast tumors (invasive lobular carcinomas, medullary carcinomas, and in situ ductal carcinomas). (cnrs.fr)
  • For separation of complex samples we apply extreme high resolution two-dimensional gel electrophoresis which theoretically allows to resolve up to 10000 protein spots in 40 x 30 cm 2DE gels. (proteome-factory.com)
  • All analyses based on electrophoresis, chromatography and mass spectrometry are done in our own labs in Berlin to ensure consistency and confidentiality. (proteome-factory.com)
  • The word proteome comes from the Australian researcher Marc Wilkins and was first mentioned on a slide in his presentation at the „ 2D Electrophoresis: from protein maps to genomes" congress in Siena on 5 September 1994. (itwreagents.com)
  • Protein separation by two-dimensional gel electrophoresis (2-DE) followed by identification of proteins using MALDI-TOF/TOF mass spectrometry (MS) was used as proteomic technique. (biomedcentral.com)
Proteomics13
  • In Proteomics Sample Preparation a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task. (chipsbooks.com)
  • New advances in mass spectrometry-based proteomics technology are having amajor impact on our understanding of how human spermatozoa acquire their capacity for fertilization. (jri.ir)
  • Recently, use of mass spectrumetrybased (MS) proteomics technology has further contributed to the identification of the proteome that make up spermatozoa (6 - 12). (jri.ir)
  • We offer fully supported contract research, fee-for-services and advanced products for gel-based and gel-free proteome analysis (proteomics), protein identification, protein & peptide sequencing, characterization, analysis and epitope mapping by mass spectrometry (MS). (proteome-factory.com)
  • As gel-free methods for global comparison of proteomes we employ LCMS-based isobaric labelling techniques like iTRAQ™ and TMT ™ proteomics or label-free nanoHPLC-ESI-MS/MS based approaches. (proteome-factory.com)
  • In plasma proteomics and CSF proteomics we deplete high abundance proteins by affinity removal before applying 2DE or gel-free proteomics methods. (proteome-factory.com)
  • Proteomics and Mass Spectrometry Core Facility provides proteomic services to the researchers from the Jagiellonian University and to external users from academia and industry. (edu.pl)
  • Scheme of the proteomic workflow and methodology available at the Proteomics and Mass Spectrometry CF. (edu.pl)
  • In shotgun proteomics, proteins are first digested with protease, and then the resulting peptides are separated by liquid chromatography and analyzed by MS. As a result, in a large-scale proteomic experiment, thousands of different proteins can be identified and quantified, which can be utilized to i.a. characterize two biological states, elucidate mechanisms of disease, detect diagnostic markers or drug targets. (edu.pl)
  • We acknowledge Proteomics and Mass Spectrometry Core Facility of the Malopolska Centre of Biotechnology, Jagiellonian University for mass spectrometry analysis. (edu.pl)
  • 07 Data-Independent Acquisition Mass Spectrometry-Based Proteomics and Software Tools: A Glimpse in 2020. (imarkerlab.com)
  • 12 Mass spectrometry-based proteomics. (imarkerlab.com)
  • 13 Next-generation proteomics: towards an integrative view of proteome dynamics. (imarkerlab.com)
Proteins12
Peptides3
Quantification5
  • 2021. "Resin Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation. (pnnl.gov)
  • Parallel ultrahigh-performance liquid chromatography separation followed by direct mass spectrometry quantification complemented the results. (cas.cz)
  • These factors can limit the reproducibility of peptide identification and quantification as well as the comprehensiveness, robustness and throughput of proteome analysis, particularly when analyzing samples of high complexity or wide dynamic range of protein concentrations as represented by tissues and body fluids 7 . (nature.com)
  • 03 MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. (imarkerlab.com)
  • 11 Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. (imarkerlab.com)
Quantitative3
  • Sequential window acquisition of all theoretical fragment ion spectra (SWATH) is a label-free mass spectrometry quantitative technique, which can retain complete fragment ion data and has good accuracy as well as reproducibility. (ncl.edu.tw)
  • Creative Biogene will provide you with an experimental report containing entire experiment procedures and proteome identification and quantitative results (bioinformatics analysis) immediately after the end of the experiment. (mitochondriasci.com)
  • In contrast to the rather static genome, the proteome and also the transcriptome are dynamic and can therefore change in their qualitative and quantitative protein composition due to changing conditions (environmental factors, temperature, gene expression, drug administration, etc. (itwreagents.com)
Electrospray1
Proteomic3
  • The proteomic techniques include mass spectrometry based protein or peptide identification and characterization. (marine-biotechnologie.de)
  • Creative Biogene provides qualitative proteomic analysis technology to identify the protein mixture in the purified samples such as IP and pull-down by liquid chromatography-mass spectrometry (LC-MS/MS), identify the target protein and its interaction protein at the same time, and construct the protein interaction spectrum with the target protein. (mitochondriasci.com)
  • We perform complete proteomic workflow that includes sample preparation, mass spectrometry measurement and data analysis. (edu.pl)
Separation2
Lipidome2
Characterization2
  • Proteome Factory is also specialized in providing full services for complete protein sequencing and characterization using a combination of N-terminal and internal Edman protein sequencing, HPLC, MSMS mass spectrometry and de novo peptide sequencing. (proteome-factory.com)
  • MS is also a powerful tool to analyze single protein providing mass determination of intact molecule, confirmation of protein identification, characterization of PTMs, detection of potential truncations and mutations. (edu.pl)
HPLC1
  • High performance liquid chromatography (HPLC) columns use higher pressures and smaller solid particles, which not only overcome some limitations of standard LC, but also allow for more effective separations and wider applications, such as pharmaceutical factory, environment, forensics, and chemical industry. (amerigoscientific.com)
Affinity2
  • An automated multilectin affinity chromatography (M-LAC) platform was utilized for glycoprotein enrichment followed by nano-LC-MS/MS analysis. (nih.gov)
  • Our packings and media mainly consist of silica gels or polymeric resins for normal phase, reversed phase, affinity, ion exchange, and hydrophilic interaction chromatography. (amerigoscientific.com)
Spectrometric3
  • Endoproteinase Glu-C, recombinant (V8) is a serine endoproteinase used with in-gel digestion and mass spectrometric analysis. (serva.de)
  • Lysyl Endopeptidase ® is especially designed for use with in-gel digestion and mass spectrometric analysis. (serva.de)
  • 08 Mass-spectrometric exploration of proteome structure and function. (imarkerlab.com)
Post-Translational3
  • This definition of proteome includes the protein expression of the individual protein, the isoforms of a protein and post-translational modifications of a protein (15). (jri.ir)
  • Wei-Jun Qian is a bioanalytical chemist whose research centers primarily on the development and applications of mass spectrometry-based approaches to better quantify the dynamic changes in protein abundances and protein post-translational modifications in biological and clinical applications. (pnnl.gov)
  • Mass spectrometry (MS) as a robust, sensitive and precise technique, enables the study of proteomes providing information on protein abundances, post-translational modifications and protein-protein interaction networks. (edu.pl)
High throughput1
  • 03 DIA-NN: neural networks and interference correction enable deep proteome coverage in high throughput. (imarkerlab.com)
Methods4
  • Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. (nhbs.com)
  • METHODS: Using peripheral blood mononuclear cells from wasp venom-allergic individuals and IL-4 ELISPOT analysis, we characterized T cell responses to whole venom and gel filtration/ion exchange-fractionated venom. (ox.ac.uk)
  • Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) data showed that bead beating and enzymatic lysis were the most efficient methods for analysis of the proteome. (qmul.ac.uk)
  • Protein Biochemistry is the collection of methods that allows science and medicine to explore and understand the proteome. (itwreagents.com)
Spectrometry analysis1
Antibodies1
  • 9] reviewed the field by focusing on antibodies, nanobodies and aptamers and asked the following question: amongst these, that are very best for deciphering the proteome of non-model species Antibodies, in particular those that happen to be monoclonal, have been utilised for some 40 years with great achievement, but due to their species specificity, they're usually not Aztreonam supplier acceptable when other non-model species are investigated. (lckinhibitor.com)
Analysis7
  • Purification of Cellular and Organelle Populations by Fluorescence-Activated Cell Sorting for Proteome Analysis - William L. Godfrey, Colette J. Rudd, Sujata Iyer and Diether Recktenwald 10. (nhbs.com)
  • Protein and peptide chromatography prior to MS analysis can partly address this issue. (hindawi.com)
  • The only far-reaching human sperm proteome analysis available to date is work done by Baker et al. (jri.ir)
  • The Centre of Functional Genomics (ZIK FunGene) of the University of Greifswald is one of the leading institutes in the field of functional genomics of bacteria with special expertise in global and functional proteome analysis. (marine-biotechnologie.de)
  • We are highly interested in cooperations in the field of proteome analysis with partners from industry or academia. (marine-biotechnologie.de)
  • cleaves specifically the peptide bonds at the carboxy-terminal side of Lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for protein sequence analysis and for proteome research. (serva.de)
  • Comparative analysis of barley leaf proteome as affected by drought stress. (uni-frankfurt.de)
Liquid2
Analyze2
  • We analyze the collected human sperm proteome by the Database for Annotation, Visualization and Integrated Discovery (DAVID) software. (jri.ir)
  • In this study, SWATH-MS is used to analyze the proteome in urine samples of FM and OA patients to investigate the mechanisms of FM and OA. (ncl.edu.tw)
Interaction1
  • Therefore, protein-protein interaction is the most basic activity in cells, called the interaction proteome (Interactome). (mitochondriasci.com)
Urine1
  • 2021. "A comprehensive urine proteome database generated from patients with various renal conditions and prostate cancer. (pnnl.gov)
Characterize1
  • OBJECTIVE: We sought comprehensively to characterize the T cell proteome for wasp venom to facilitate the future development of T cell-based immunotherapeutic approaches. (ox.ac.uk)
Defining2
Spectra1
  • 02 Prosit: proteome-wide prediction of peptide tandem mass spectra by deep learning. (imarkerlab.com)
Defined as the protein com1
  • Proteome definition: The proteome has been defined as the protein complement of the genome. (jri.ir)
Genome2
  • A caterpillar and the butterfly that develops from it contain the same genome, but nevertheless differ externally due to a different proteome. (itwreagents.com)
  • Similar to the Human Genome Organisation (HUGO), the researchers of the International Human Proteome Organisation (HUPO) share the workload worldwide. (itwreagents.com)
Tissues1
  • ProtTalks-DB Provide a comprehensive proteome database with 234 human tissues, 234 brain regions, and 45 mouse tissues. (imarkerlab.com)
Sample Preparation1
  • These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. (nhbs.com)
Responses1
  • T cell responses to gel filtration/ion exchange fractionated venom were dominated by responses to phospholipase A(1), hyaluronidase and antigen 5. (ox.ac.uk)
Bioinformatics1
  • The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. (nhbs.com)
Spectrometer1
  • It is a hybrid mass spectrometer, which combines high-performance quadrupole precursor selection with high-resolution, accurate-mass Orbitrap™ detection. (edu.pl)
Human3
  • In this short review, we have collected the human sperm proteome from the literature and analyzed it by the Database for Annotation, Visualization and Integrated Discovery (DAVID) software. (jri.ir)
  • Additionally, the majority of the collected human sperm proteome belonged to cytoplasm. (jri.ir)
  • Through these pathways, we can better understand the effects of FM and OA on human proteome. (ncl.edu.tw)
Research4
Mice2
  • Effects of insulin and the glucagon-like peptide 1 receptor agonist liraglutide on the kidney proteome in db/db mice. (lu.se)
  • Prot Rhythm provide an eight-tissue circadian proteome atlas of WT and Per1/Per2 DKO mice. (imarkerlab.com)
Journal1
Detection1
  • 2021. "Mass spectrometry-based direct detection of multiple types of protein thiol modifications in pancreatic beta cells under endoplasmic reticulum stress. (pnnl.gov)