We use a combination of biochemical and cell-based assays to show that dystroglycan proteolysis is conformationally regulated by an extracellular, juxtamembrane "proteolysis domain", comprised of tandem Ig-like and SEA-like domains. (biorxiv.org)
It is anticipated that the proposed research will provide fundamental insight into how Nrxn-1 deletions give rise to human neurodevelopmental disorders and will offer the first functional analysis of extracellular proteolysis as a regulatory mechanism of neurexin-containing adhesion complexes. (grantome.com)
The goal of the proposed research is determine the biological function of Nrxn1 at synapses and to evaluate potential regulatory mechanisms of its function, particularly through extracellular proteolysis. (grantome.com)
Ligand-dependent proteolysis at the S2 site removes the bulk of the extracellular domain of Notch. (ulaval.ca)
Intramembrane2
Subsequent γ-secretase-mediated intramembrane proteolysis of the remaining membrane-tethered Notch fragment at the S3 site produces a nuclear-destined Notch intracellular domain (NICD). (ulaval.ca)
Taking a position on intramembrane proteolysis. (bvsalud.org)
Regulation1
Consequently, abnormality in the regulation of proteolysis can cause disease. (wikipedia.org)
Proteolysis can also be used as an analytical tool for studying proteins in the laboratory, and it may also be used in industry, for example in food processing and stain removal. (wikipedia.org)
Limited proteolysis of a polypeptide during or after translation in protein synthesis often occurs for many proteins. (wikipedia.org)
Proteolysis can, therefore, be a method of regulating biological processes by turning inactive proteins into active ones. (wikipedia.org)
The ubiquitinated protein is then degraded by the 26S proteasome, a protein complex that degrade proteins by proteolysis. (greek.doctor)
Allergenicity of food proteins has been correlated to their structural conformation and their resistance to proteolysis. (cea.fr)
In this report, we modify VHL to engineer an Affinity directed PROtein Missile (AdPROM) system to direct specific endogenous target proteins for proteolysis in mammalian cells. (dundee.ac.uk)
Oxidation works in concert with proteolysis, improving N liberation from proteins in SOM. (lu.se)
Peptide bonds1
Proteolysis is the chemical breaking of peptide bonds. (greek.doctor)
Assays1
Compared with mass spectrometry-based high-throughput stability assays, cDNA display proteolysis achieves a 100-fold larger scale and can easily be applied to study mutational libraries that pose difficulties for proteomics," the authors report. (genomeweb.com)
Endothelial1
Elastase-mediated proteolysis of endothelial cell cadherins may represent the mechanism through which lung microvascular integrity is disrupted after intestinal I-R. (nih.gov)
Mutations4
For the study, the authors used this cDNA display proteolysis strategy to systematically come up with about 776,000 high-quality folding stability measurements for hundreds of naturally occurring or engineered protein domains containing single or double amino acid mutations under consistent conditions, for example, making it possible to consider everything from the folding stability contributions of specific amino acids to the interactions affected by protein domain folding stability-related selective pressures. (genomeweb.com)
The intact proteolysis domain is resistant to MMP cleavage, but structurally-disruptive muscular dystrophy-related mutations sensitize dystroglycan to proteolysis. (biorxiv.org)
Intriguingly, previously uncharacterized cancer-associated mutations that map to the proteolysis domain similarly lead to increases in proteolysis and rates of cell migration, potentially revealing a new pathogenic mechanism in cancer. (biorxiv.org)
Many mechanisms of protein function contribute to amyloidogenesis, including "nonphysiologic proteolysis, defective or absent physiologic proteolysis, mutations involving changes in thermodynamic or kinetic properties, and pathways that are yet to be defined. (medscape.com)
Digestion2
Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. (wikipedia.org)
Proteolysis was accompanied by an increase in the ATPase activity of the digest: by centrifuging down the insoluble digestion products it was shown that digestion resulted in the appearance of ATPase activity in the soluble phase with a concomitant decrease in ATPase activity in the pellet fraction. (rupress.org)
Protease1
The high-resolution structure of ovine placental lactogen (oPL) and ovine prolactin (oPRL), not yet established in detail, was probed by limited proteolysis with the Glu-specific protease from Staphylococcus aureus V8. (illinois.edu)
Endogenous2
To determine whether fasting alters the ability of insulin to suppress endogenous glucose production or proteolysis, isotopic flux measurements of glucose, leucine, alpha-keto-isocaproate, and alanine were made in 13 normal volunteers after a 14-h (day 1) and an 84-h (day 4) fast. (nih.gov)
In conclusion, insulin suppresses proteolysis equally well before and after brief fasting, and endogenous glucose production is more completely suppressed by insulin after brief fasting than after an overnight fast. (nih.gov)
Determinant1
Thus, it seems that the dimeric structure is a major determinant of nNOS stability and proteolysis. (aspetjournals.org)
Staphylococcus1
The aim of this project was to analyse the effect of mastitis pathogen Staphylococcus (S.) aureus on proteolysis in UHT milk. (slu.se)
Cleavage1
While in hGH there were no cleavage sites inside of any of the four α- helices, the analysis of the fragments obtained after partial proteolysis of oPL showed a site of cleavage at the putative third helix, suggesting that this helix is partially unwound at this point. (illinois.edu)
Contributes1
We also describe how the proteolytic activities are determined by different platelet populations in a thrombus and conversely how proteolysis contributes to the formation of such populations. (frontiersin.org)
Stability3
Using their high-throughput complementary DNA (cDNA) display proteolysis assay, the investigators report they can gauge protein folding stability patterns in a library of up to 900,000 sequences per week with a reagent price tag of around $2,000. (genomeweb.com)
Compared with the previous yeast display proteolysis method, cDNA display proteolysis resolves a wider dynamic range of stability and is more reproducible even at a 50-fold larger experimental scale. (genomeweb.com)
proteolysis_c-Jun N-terminal kinase (JNK) cooperates with Gsk3beta to regulate Dishevelled-mediated microtubule stability. (proteolysis.jp)
Pathway1
These results argue that photoreceptor differentiation is regulated by the RAS pathway through targeted proteolysis of the TTK repressor. (psu.edu)
Migration1
Moreover, increased dystroglycan proteolysis correlates with faster cell migration, linking proteolysis to a disease-relevant cellular phenotype. (biorxiv.org)
Amino acids1
Complete proteolysis of a protein results in the amino acids the protein was built up of. (greek.doctor)
Differentiation2
We show that H3 monomethylation at lysine 27 (H3K27me1) is essential for MMP-9-dependent H3NT proteolysis during RANKL-induced osteoclast differentiation. (biomedcentral.com)
Through the recognition of H3K27me1 mark, MMP-9 localizes and generates H3NT proteolysis at the genes encoding osteoclast differentiation factors. (biomedcentral.com)
Genes2
By using RNAi and small molecule inhibitor approaches, we also confirmed that G9a is the major methyltransferase to catalyze H3K27me1 for MMP-9-dependent H3NT proteolysis and trigger the expression of osteoclast-specific genes. (biomedcentral.com)
TNF-alpha induced increased expression of genes coding mediators of apoptosis, cell-matrix interactions, proteolysis and C-C chemokines. (cdc.gov)
Nitrogen2
and stored for 56 d at 4 ◦C. Proteolysis was assessed using water-soluble nitrogen (WSN), trichloroacetic acid-soluble nitrogen (TCASN), phosphotungstic-soluble nitrogen (PTA-SN), urea polyacrylamide gel electrophoresis (urea-PAGE), and peptide patterns. (edu.au)
Organic nitrogen mining by ectomycorrhizal fungi has until now only been attributed to proteolysis. (lu.se)
Mass1
Here, we have investigated the suitability of Lys-N proteolysis for use with MALDI mass spectrometry to characterize the yeast Arp2 complex and E. coli PAP I protein interactions. (princeton.edu)
Effect3
The effect of solution composition and enzymic proteolysis on axonemes prepared from the sperm of sea urchins, Tripneustes gratilla , has been investigated. (rupress.org)
The main results of the team are the lack of cross-reactivity between allergens with strong sequence homologies, the relationship between the structure, resistance to proteolysis and allergenicity and the effect of technological processes and composition of the matrix on digestibility and allergenicity. (cea.fr)
The effect of substitution of NaCl with Potassium chloride (KCl) in brine solution on proteolysis of halloumi cheese was investigated. (edu.au)
Activity1
In addition, proteolysis increases the specific ATPase activity of dynein and removes a portion of it from the axonemal structure. (rupress.org)
Function1
The sequential and regulated proteolysis by γ-secretase and MIPEP suggests a new means by which Notch function can be modulated. (ulaval.ca)
Solution1
Observations on specimens prepared for electron microscopy by thin sectioning allowed a rough correlation to be made between the dark-field observations, chemical analyses, and morphological alterations attendant with the proteolysis and solution conditions. (rupress.org)
Research1
The ISFP was founded to stimulate scientific research on proteolysis relevant to human biology and pathology. (fibrinolysis.org)
Structure1
The partial proteolysis of the rest of the molecule was compatible with a similar folding pattern for oPL, hGH and pGH, on the basis of the crystal structure of these last hormones. (illinois.edu)
Rate1
Leucine rate of appearance (an index of proteolysis) was greater on day 4 than on day 1 (P less than 0.001), but decreased to equal values during the pancreatic clamp on each study day. (nih.gov)
Expression1
Our data establish new functions for G9a-mediated H3K27me1 in MMP-9-dependent H3NT proteolysis and demonstrate how histone modification can be exploited to regulate osteoclastogenic gene expression at the molecular level. (biomedcentral.com)