• The current study describes the implementation of a hybridization liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform for the direct quantitation of antisense oligonucleotide (ASO) ƒ u . (aspetjournals.org)
  • Sal-T was measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Linear and quantile regression analyses were used to determine the age-specific 2.5th and 97.5th percentiles for the general population (1675 men and 2453 women) and the population with health exclusions (1145 men and 1276 women). (medscape.com)
  • Kocher, T. & Superti-Furga, G. Mass spectrometry-based functional proteomics: from molecular machines to protein networks. (nature.com)
  • Genomics and proteomics have revolutionized biology but would not have been possible without developments in capillary sequencing, cDNA microarrays, and mass spectrometry, amongst other enabling technologies. (nih.gov)
  • ABSTRACT In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. (who.int)
  • The study shows the usefulness of proteomics in identifying proteins that may express differences between sensitive and resistant L. tropica isolates. (who.int)
  • These nanoparticles enable the direct enrichment and analysis by MALDI-TOF mass spectrometry. (fraunhofer.de)
  • By MALDI-TOF mass spectrometry, we identified this protein to be cMyBP-C. These data were confirmed by immunostaining using the p-PKD-Ser744/748 antibody, which displayed a striated pattern similar to the one observ ed for a regular cMyBP-C antibody. (scirp.org)
  • In this publication we report on the development of a quantitative enzymatic method for the detection of four botulinum neurotoxin (BoNT) serotypes responsible for human botulism by MALDI-TOF mass spectrometry. (cdc.gov)
  • Capture Compounds are trifunctional small molecules to reduce the complexity of the proteome by functional reversible small molecule-protein interaction followed by photo-crosslinking and purification. (jove.com)
  • Historically, ƒ u determination for small-molecule drug candidates has been determined by ultrafiltration, ultracentrifugation, or equilibrium dialysis, which are techniques based on the physical separation of unbound drug molecules from those bound to proteins ( Pacifici and Viani, 1992 ). (aspetjournals.org)
  • A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
  • The basic format of the yeast-two hybrid system involves the creation of two hybrid molecules, one in which the " bait ' protein is fused with a transcription factor, and one in which the " prey " protein is fused with a related transcription factor . (genomicglossaries.com)
  • When a virus enters a cell, viral and cellular protein molecules begin to interact. (tum.de)
  • A new technique that can analyse how drug molecules bind to proteins in tissue samples could offer an improved route to drug discovery and development. (birmingham.ac.uk)
  • It uses mass spectrometry, an analytical tool commonly used for identifying the properties of molecules within a sample. (birmingham.ac.uk)
  • Because aldehydes have the tendency to react with biological molecules to form various products, including Schiff base protein adducts, free aldehydes released into the headspace of biological samples from the Schiff base protein adducts at low pH (~3) are analyzed. (cdc.gov)
  • Our service comes with a full package - from construct design, gene cloning and expression, custom protein production , protein characterization to crystal hit screening and optimization, x-ray diffraction data collection and structure solving. (jubilantbiosys.com)
  • We describe the proteomic characterization of mouse brain Ca2+-calmodulin-binding proteins that were purified using calmodulin affinity chromatography. (lu.se)
  • Using a proteomic approach that coupled DNA affinity chromatography and mass spectrometry, the researchers identified a number of proteins that bind to HIV DNA, including IFI16. (genomeweb.com)
  • Here, we, therefore, applied affinity chromatography together with mass spectrometry to isolate and identify Rp-8-Br-PET-cGMPS interactors from retinas derived from three different murine RP models (i.e., rd1, rd2, and rd10 mice). (lu.se)
  • Proteins in the eluates from four different affinity chromatography experiments were identified by 1-DE and in-gel digestion followed by LC-MS/MS. Parallel experiments were performed using two related control-proteins belonging to the EF-hand family. (lu.se)
  • Based on primary sequence comparisons, β subunits are predicted to be modular structures composed of five domains (A-E) that are related to the large family of membrane-associated guanylate kinase proteins. (jneurosci.org)
  • The Parkinson's disease genes pink1 and parkin , which encode a mitochondrially targeted protein kinase, and an E3 ubiquitin ligase, respectively, participate in a key mitochondrial quality-control pathway that eliminates damaged mitochondria. (sdbonline.org)
  • The former is capable of binding to protein kinase C, an enzyme that binds to the membrane to regulate the speed of exocytosis. (news-medical.net)
  • One of the upstream kinases, which phosphorylate cMyBP-C, is protein kinase D (PKD). (scirp.org)
  • During this degeneration, cyclic guanosine-3′,5′-monophosphate (cGMP) levels become elevated, leading to over-activation of the cGMP-binding protein cGMP-dependent protein kinase (PKG). (lu.se)
  • Image analysis of the matched maps identified 7 proteins that were either over- or down-expressed: activated protein kinase c receptor (LACK), alpha tubulin (×2), prostaglandin f2-alpha synthase, protein disulfide isomerase, vesicular transport protein and a hypothetical protein. (who.int)
  • Selective microfluidics-based ligand enrichment followed by sequencing, or SMiLE-seq, combines antibody arrays, mechanical trapping, and next-generation sequencing readouts to provide a new platform for characterizing DNA-protein interactions. (genomeweb.com)
  • That year, the two published a paper in Science describing a method to trap low-affinity DNA-protein interactions, specifically those of transcription factors. (genomeweb.com)
  • The transcription factors bind to any number of DNA motifs in the library, those interactions are trapped, and then the readout matches up each motif with the transcription factor via the barcode. (genomeweb.com)
  • Deplancke also worked in advanced bioinformatics to derive predictive models for binding interactions. (genomeweb.com)
  • Affinity purification-mass spectrometry and network analysis to understand protein-protein interactions. (nature.com)
  • co-immunoprecipitation Used to determine protein- protein interactions . (genomicglossaries.com)
  • A mass spectrometer device (detail): Using mass spectrometric analysis at the Max Planck Institute of Biochemistry, the researchers discovered 1484 interactions between viral and human cellular proteins. (tum.de)
  • The result is a freely accessible datase t that provides information on which cellular proteins the viral proteins bind to and the effects of these interactions on the cell. (tum.de)
  • In total, 1484 interactions between viral proteins and human cellular proteins were discovered. (tum.de)
  • Many cellular processes are based on such interactions between proteins. (eurekalert.org)
  • To investigate antigen-antibody interactions and assess the potential of the most common epitope mapping techniques, we generated a series of mAbs against factor H binding protein (fHbp), a key virulence factor and vaccine antigen of Neisseria meningitidis. (rcsb.org)
  • We can isolate soluble as well as membrane bound protein complexes and study their assembly, and can also purify heterologously expressed proteins from bacteria in order to study protein-protein interactions or ubiquitination reactions in vitro. (ruhr-uni-bochum.de)
  • The method provides substantial improvements, including minimal matrix effects and high specificity when compared with previously used oligonucleotide ƒ u detection methods such as ligand binding assays or liquid scintillation. (aspetjournals.org)
  • The hybridization LC-MS/MS platform provided unique advantages, including minimal matrix effects and high specificity, compared with traditional ligand binding assays or liquid scintillation approaches, which enabled efficient and reliable in vitro protein binding assay. (aspetjournals.org)
  • To therapeutically target B7-H3 in AML cells, we have generated four monoclonal antibodies that binds B7-H3 with unique specificity and possess immuno-modulatory function. (lls.org)
  • p class=\'abstract\'>BACKGROUND: Conventional hemodialysis provides limited clearance of uremic solutes that bind to plasma proteins. (stanford.edu)
  • Here we use a Capture Compound with S -adenosyl- L -homocysteine-binding as selectivity function to isolate methyltransferases from an Escherichia coli whole cell lysate and identify them by MS. (jove.com)
  • We will perform protein pull down assays to isolate B7-H3 binding proteins in natural killer cells followed by mass spectrometry to identify the B7-H3 receptor. (lls.org)
  • In addition, the effectiveness of antitoxin treatment may vary depending on circulating toxin levels, so that rapid identification and quantification of BoNT proteins in clinical specimens and food is desirable for optimization of treatment. (cdc.gov)
  • This study generated additional information on TTP phosphorylation using phosphopeptide mapping and mass spectrometry. (usda.gov)
  • This study aimed to generate additional information on TTP phosphorylation using phosphopeptide mapping and mass spectrometry (MS). Wild-type and site-directed mutant TTP proteins were expressed in transfected human cells followed by in vivo radiolabeling with [32P]-orthophosphate. (usda.gov)
  • Protein phosphorylation plays an important role in physiological processes, such as muscle contraction. (scirp.org)
  • Phosphorylation of cMyBP-C is essential for normal cardiac function, since dephosphorylation of this protein leads to its degradation and has been associated with cardiomyopathy. (scirp.org)
  • Cohen, P. (2002) The origins of protein phosphorylation. (scirp.org)
  • Barefield, D. and Sadayappan, S. (2010) Phosphorylation and function of cardiac myosin-binding protein c in health and disease. (scirp.org)
  • FUS/TLS was independently identified as the hnRNP P2 protein, a subunit of a complex involved in the maturation of pre-mRNA. (wikipedia.org)
  • The flavin together with the iron-sulfur clusters function to mediate electron transfer from the dicarboxylate binding site on the Fp subunit to the membrane-anchor domain where quinone is reduced to quinol. (lu.se)
  • Flavinylation of Fp in B. subtilis does not require the Ip subunit or the membrane anchor but depends on folding of the Fp polypeptide into a state that can bind FAD and subsequent mediate covalent binding of the FAD [3, 4]. (lu.se)
  • The highly-experienced structural biology team at Jubilant Biosys supports structure-based drug discovery from gene to protein co-crystal structures with small-molecule compounds at high resolution using x-ray crystallography. (jubilantbiosys.com)
  • Although a 12-residue epitope in the C terminus of fHbp was identified by both Peptide Scanning and Phage Display Library screening, other approaches, such as hydrogen/deuterium exchange mass spectrometry (MS) and X-ray crystallography, showed that mAb 12C1 occupies an area of ∼1,000 Å(2) on fHbp, including >20 fHbp residues distributed on both N- and C-terminal domains. (rcsb.org)
  • The method reproducibly hydrolyzes aldehyde adducts covalently bound to proteins, using automation to individually incubate samples prior to analysis with hydrochloric acid (Yeo 2004). (cdc.gov)
  • The covalent bond to FAD in SQR is essential for enzyme activity and the main reason for this is that the bond raises the midpoint redox potential of the flavin by more than 80 mV compared to non-covalently bound flavin. (lu.se)
  • SQR in E. coli and B. subtilis can be assembled in the membrane without covalently bound FAD but are enzymatically inactive [3, 4, 6]. (lu.se)
  • The B. subtilis Fp polypeptide was found to lack covalently bound FAD when produced in E. coli although E. coli Fp in the same cell was flavinylated [8]. (lu.se)
  • It is unexpected that B. subtilis SQR without covalently bound FAD is not assembled in the E. coli membrane provided that all other aspects of assembly, including iron-sulfur cluster biogenesis, are functional in the heterologous system. (lu.se)
  • Notably, certain B. subtilis mutants with mutated Fp lack covalently bound flavin and do not assemble the SQR polypeptides in the membrane [3]. (lu.se)
  • The slight difference in electrophoretic mobility of normal Fp and that produced in E. coli could be due to the absence of covalently FAD but could have another explanation because this difference was not observed for some mutant Fp without covalently bound FAD [3]. (lu.se)
  • In our previous study we established an analytical method for the identification of intracellular cisplatin-binding proteins. (nih.gov)
  • Based on this analytical method we extended the identification of intracellular cisplatin-protein adducts to the alkaline pH range (pH 6-10) giving chance to discover new important binding partners. (nih.gov)
  • An automated analytical method was developed using solid phase microextraction (SPME) gas chromatography (GC) and high-resolution mass spectrometry (HRMS) with selected ion mass detection and isotope-dilution techniques. (cdc.gov)
  • This analytical method quantifies trace levels of 12 aldehydes from protein adducts in human serum. (cdc.gov)
  • At the sub-cellular scale, maps document the spatial organization of proteins, RNA, DNA, and metabolites with nanometer precision and temporal acuity on the order of seconds. (stanford.edu)
  • The biological system is complex with many regulatory features such as DNA, mRNA, proteins, metabolites, and epigenetic features such as DNA methylation and histone post-translational modifications (PTMs). (rsc.org)
  • A rapid method for the separation of vitamin D and its metabolites by ultra-high performance supercritical fluid chromatography-mass spectrometry. (lu.se)
  • They used mass spectrometry on thin sections of tissue to detect the drug molecule and the specific fatty acid binding protein to which it attaches to form a complex. (birmingham.ac.uk)
  • In terms of predicting where in the genome the transcription factors bind, the models we're able to derive seem to be, in general, actually better than the other assays. (genomeweb.com)
  • We also provide recombinant proteins and cells expressing target proteins for functional assays. (jubilantbiosys.com)
  • Jubilant Biosys offers high-quality proteins for enzymatic assays, high throughput screening, biophysical and structural studies. (jubilantbiosys.com)
  • Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
  • In a paper published in Nature Methods this week, a Switzerland-based team of researchers, led by bioengineer Bart Deplancke of the École Polytechnique Fédérale de Lausanne (EPFL), showcased the assay's ability to determine DNA-binding specificities for transcription factors. (genomeweb.com)
  • Also in Science , researchers led by the University of California, San Francisco's Warner Greene report that interferon-gamma-inducible protein 16 acts as a host DNA sensor that triggers an immune response and CD4 T cell death in response to an abortive HIV infection. (genomeweb.com)
  • This new technique, described in a paper published today (14 July 2022) in Angewandte Chemie, enables researchers to use real tissue samples to assess which proteins the drug will bind to in the body and therefore how effective it is likely to be against the target. (birmingham.ac.uk)
  • Analytes are then separated in a capillary column and quantified using multiple ion detection (MID) with mass spectrometry. (cdc.gov)
  • Mathematical modeling predicted the dependence of solute clearances on the extent to which solutes were taken up by the carbon block and were bound to plasma proteins.RESULTS: By itself, the conventional system provided clearances of the tightly bound solutes p-cresol sulfate (PCS) and indoxyl sulfate (IS) of only 18±10 and 19±11 ml/min, respectively (mean±sd). (stanford.edu)
  • Because ionized calcium is the only component of the total serum calcium level that is regulated by calciotropic hormones, decisions on the total serum calcium concentration should not be made unless changes in concentrations of plasma proteins, particularly albumin, are considered. (medscape.com)
  • Introducción: El 4 y el 5 de noviembre se celebró en Madrid la XV edición de la Reunión Post-ECTRIMS, donde neurólogos expertos en esclerosis múltiple (EM) resumieron las principales novedades presentadas en el congreso de ECTRIMS 2022, celebrado en Ámsterdam entre el 26 y el 28 de octubre. (bvsalud.org)
  • Además, se muestran los resultados de algunos de los últimos estudios realizados con cladribina oral y evobrutinib presentados en el ECTRIMS 2022. (bvsalud.org)
  • Some genetic changes even affect intracellular protein transport through this process. (eurekalert.org)
  • Intracellular binding of cisplatin to proteins has been associated with acquired resistance to chemotherapy. (nih.gov)
  • The method used a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue (CFDA-cisplatin), two-dimensional gel electrophoresis (2DE) and mass spectrometry, which allows detecting and identifying intracellular CFDA-cisplatin-containing protein adducts in the acidic pH range (pH 4-7). (nih.gov)
  • The establishment of an optimized IEF protocol for basic proteins enabled us to identify several intracellular CFDA-cisplatin-binding proteins including enzymes of the glucose and serine metabolism like alpha enolase and D-3-phosphoglycerate 1-dehydrogenase. (nih.gov)
  • We are interested in the regulation of intracellular trafficking events and have a special focus on the functional role of covalent modifications of proteins and lipids. (ruhr-uni-bochum.de)
  • 1994). High-res- belongs to the family of intracellular lipid bind- olution crystal structures (Sacchetti ni et al. (lu.se)
  • MS analysis identified the peptide that was missing in the S90 and S93 mutant protein as LGPELSPSPTSPTATSTTPSR (corresponding to amino acid residues 83-103 of human TTP). (usda.gov)
  • We assume to use unique peptide for each protein. (bioconductor.org)
  • TRUE will remove the proteins which have only 1 peptide and charge. (bioconductor.org)
  • Each serotype of BoNT hydrolyzes their protein substrates at different peptide bonds. (cdc.gov)
  • Lysine-specific crosslinking mass spectrometry provided distance constraints to select a homology model of the DNAJB6 monomer, which was subsequently used in crosslink-assisted docking to generate a dimer model, revealing that a peptide-binding cleft lined with S/T-residues is formed at the monomer-monomer interface. (lu.se)
  • Bacteria often can't support over-expression needed to get workable quantities of protein for an assay, and in vitro expression often can't synthesize a fully functional transcription factor. (genomeweb.com)
  • Consistently, in vitro studies have shown that FUS/TLS binds RNA, single-stranded DNA and (with lower affinity) double-stranded DNA. (wikipedia.org)
  • however, using in vitro selection (SELEX), a common GGUG motif has been identified in approximately half of the RNA sequences bound by FUS/TLS. (wikipedia.org)
  • FUS/TLS has also been proposed to bind human telomeric RNA (UUAGGG)4 and single-stranded human telomeric DNA in vitro. (wikipedia.org)
  • SIGNIFICANCE STATEMENT A novel hybridization LC-MS/MS-based approach was successfully developed for the determination of ASO in vitro protein binding in plasma, and for the first time brain and CSF. (aspetjournals.org)
  • NEW YORK (GenomeWeb) - A new microfluidics-driven DNA-protein binding assay has its creators dreaming of launching a startup to cash in on several potential applications. (genomeweb.com)
  • The assay could help accelerate efforts to map where transcription factors bind the genomes of humans and model organisms, Deplancke said. (genomeweb.com)
  • Taking advantage of the ability of chiral protein environments to induce optical activity, circular dichroism (CD) spectroscopy was employed to characterize the alkaloid-protein non-covalent adducts. (jascoinc.com)
  • These toxins inhibit the release of neurotransmitters at the neuromuscular junction by cleaving soluble NSF attachment protein receptor (SNARE) complex proteins in nerve cells, which prevents the fusion of neurotransmitter containing vesicles to cell membranes 2 . (cdc.gov)
  • If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)
  • RNA-binding protein FUS/TLS (FUsed in Sarcoma/Translocated in LipoSarcoma), also known as heterogeneous nuclear ribonucleoprotein P2 is a protein that in humans is encoded by the FUS gene. (wikipedia.org)
  • FUS gene rearrangement has been implicated in the pathogenesis of myxoid liposarcoma, low-grade fibromyxoid sarcoma, Ewing sarcoma, and a wide range of other malignant and benign tumors (see FET protein family). (wikipedia.org)
  • The Jubilant Biosys team has demonstrated its strength in protein engineering to suit the needs of crystallization by designing multiple constructs for a given gene-to-structure project and worked on ~40 different protein targets and delivered more than 250 co-crystal structures (better than 2.5Å resolution). (jubilantbiosys.com)
  • A novel cross-phylum family of proteins comprises a KRR1 (YCL059c) gene which is essential for viability of Saccharomyces cerevisiae cells. (nih.gov)
  • An example is a mutation in the gene for the Glut1 protein that causes two specific building blocks of protein, namely leucines, to lie next to one another, creating a so-called dileucine motif. (eurekalert.org)
  • High density arrays of extraordinarily sensitive integrated microring resonators will allow many gene and protein signatures to be simultaneously quantitated from a single patient sample. (nih.gov)
  • In this review, we discuss considerations of the study design for each data feature, the limitations in gene and protein abundance and their rate of expression, the current data integration methods, and microbiome influences on gene and protein expression. (rsc.org)
  • This gene encodes a nucleolar transcriptional regulator that was first identified by its ability to bind specifically to the Myb proto-oncogene protein. (nih.gov)
  • Functional organization of the yeast proteome by systematic analysis of protein complexes. (nature.com)
  • The main question in both cases concerns the functional role of the posttranslational modification of proteins with ubiquitin, as well as the impact of signalling lipids like phosphatidylinositol 3-phosphate (PtdIns3P). (ruhr-uni-bochum.de)
  • This study provides a functional framework for studies on these previously uncharacterized proteins. (lu.se)
  • Human serum albumin (HSA) binding of anticancer plant alkaloid ellipticine has been studied in conjunction with assessing its association to serum a1-acid-glycoprotein (AAG). (jascoinc.com)
  • hence, serum free testosterone is usually derived from mathematical formulas using association constants of testosterone with its binding proteins. (medscape.com)
  • Unless serum proteins contain abnormalities, total serum calcium concentration is normally between 8.5 and 10.2 mg/dL of serum. (medscape.com)
  • Total serum calcium is less difficult to measure than the ionized calcium component is, and ionized calcium measurements are rarely needed if serum protein concentrations can be measured. (medscape.com)
  • In patients multiple myeloma, the globulin concentration is often increased, leading to excessive binding of calcium to the monoclonal paraprotein and occasional elevation of the total serum calcium concentration, yet the ionized calcium level may be normal in these individuals. (medscape.com)
  • Additionally, FUS/TLS has been found to bind a relatively long region in the 3′ untranslated region (UTR) of the actin-stabilising protein Nd1-L mRNA, suggesting that rather than recognising specific short sequences, FUS/TLS interacts with multiple RNA-binding motifs or recognises secondary conformations. (wikipedia.org)
  • A total of 140 putative Ca2+-calmodulin-binding proteins were identified of which 87 proteins contained calmodulin-binding motifs. (lu.se)
  • Among the 87 proteins that contained calmodulin-binding motifs, 48 proteins have not previously been shown to interact with calmodulin and 39 proteins were known calmodulin-binding proteins. (lu.se)
  • Alberts, B. The cell as a collection of protein machines: preparing the next generation of molecular biologists. (nature.com)
  • Owing to their physical properties, such as relatively high molecular weight, linear structure, and nonspecific binding, ASOs present unique challenges in ƒ u determination through traditional techniques. (aspetjournals.org)
  • Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. (genomicglossaries.com)
  • To this end, we have generated recombinant B7-H3 protein that is conjugated with a molecular tag. (lls.org)
  • Molecular basis of peroxisomal biogenesis disorders caused by defects in peroxisomal matrix protein import. (ruhr-uni-bochum.de)
  • One of the key molecular events in skin sensitization is protein haptenation, i.e. the chemical modification of self-skin protein(s) thus forming macromolecular immunogens. (cdc.gov)
  • The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)
  • RNA viruses may escape acquired humoral and cellular immune responses by mutations in protective antigenic epitopes (e.g., avian influenza viruses), while accessory nonstructural proteins or multifunctional structural proteins interfere with the interferon system (e.g. (imperial.ac.uk)
  • By utilizing physiological protein levels representative of plasma and saliva, cellular systems are able to closely predict the in vivo saliva/blood concentration ratio (0.021 vs. 0.049) of 3,5,6-trichloro-2-pyridinol (TCPy), the major chemical-specific metabolite of chlorpyrifos. (cdc.gov)
  • Kinetic descriptions of protein binding were added to the cellular computational model and parameterized with data of TCPy protein binding in cell culture medium. (cdc.gov)
  • Miniscule changes in previously little-noticed flexible segments of the Glut1 protein could lead to severe cellular disturbances - other genetic disorders might be caused by the same mechanism. (eurekalert.org)
  • Malformed and contorted, the protein can no longer carry out its task in the cellular machinery and thus triggers the syndrome. (eurekalert.org)
  • But some of the mutated proteins completely lost this ability or bound to other proteins and thus disrupted the operation of the cellular machinery. (eurekalert.org)
  • The cellular apparatus involved in pinching off vesicles from the cell membranes and transporting them into the cell's interior via endocytosis is partially responsible for misrouting the Glut1 protein. (eurekalert.org)
  • The encoded protein is thought to play a role in many cellular processes including response to nucleolar stress, tumor suppression and synthesis of ribosomal DNA. (nih.gov)
  • Regulation and function of Myb-binding protein 1A (MYBBP1A) in cellular senescence and pathogenesis of head and neck cancer. (nih.gov)
  • Recombinant protein services helped customers' need for high throughput screening activity. (jubilantbiosys.com)
  • Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)
  • Our preliminary study using 2D isolates were recovered from liq- new cases throughout the world cu- gel electrophoresis in drug sensitive/ uid nitrogen, and sub-cultured in taneous leishmaniasis (CL) remains resistant strains of L. tropica showed RPMI1640 medium (Gibco/BRL) a serious public health problem in that some proteins were differentially supplemented with 10% fetal bovine numerous countries [1,2]. (who.int)
  • Implications of protein binding for predicting pesticide salivary transport using a combination experimental and computational approach. (cdc.gov)
  • Here, we hypothesize that protein binding plays an important role in TCPy salivary transport. (cdc.gov)
  • Overall, these experiments demonstrate the importance of protein binding for salivary clearance of TCPy. (cdc.gov)
  • This combination experimental and computational approach could serve as a platform to predict salivary transport of chemicals highly bound to proteins. (cdc.gov)
  • however, a substantial systematic positive bias was present in women, which might reflect the influence of salivary protein binding to the lower female concentrations of Sal-T. [ 8 ] Whether Sal-T can be a surrogate for circulating free testosterone or a valid measure of tissue bioavailable testosterone can now be investigated further. (medscape.com)
  • Here, we propose targeting B7-H3 (CD276), a promising immune checkpoint protein that has been reported to inhibit immune cells function by binding its receptor on natural killer (NK) cells and T lymphocytes. (lls.org)
  • By identifying which proteins it interacts with scientists will be able to predict at an earlier stage whether or not it will have the desired therapeutic effect. (birmingham.ac.uk)
  • Tristetraprolin (TTP), an anti-inflammatory protein, binds to the AREs of clinically important mRNAs such as TNF mRNA and promotes the destruction of those transcripts. (usda.gov)
  • In these instances, the promoter and N-terminal part of FUS/TLS is translocated to the C-terminal domain of various DNA-binding transcription factors (e.g. (wikipedia.org)
  • Beyond nucleic acid binding, FUS/TLS was also found to associate with both general and more specialized protein factors to influence the initiation of transcription. (wikipedia.org)
  • The obvious next step is to integrate this information and learn how genes, proteins, and/or epigenetic factors influence the phenotype of a disease in context of the system. (rsc.org)
  • Tristetraprolin/zinc finger protein 36 (TTP/ZFP36) binds and destabilizes some pro-inflammatory cytokine mRNAs. (usda.gov)
  • The zinc species inside the cell could slow down the transport protein that loads the dopamine into the vesicles," suggests Ewing. (news-medical.net)
  • The light chain is a zinc-metalloprotease which is responsible for the cleavage of three SNARE complex proteins including SNAP-25 (Synaptosome-associated protein of 25 kDa), synaptobrevin-2 (also termed VAMP-2) and syntaxin. (cdc.gov)
  • After comparing the results from the different experiments, we were able to exclude a significant number of proteins suspected to bind in a nonspecific manner. (lu.se)
  • TTP is highly phosphorylated in vivo and is a substrate for several protein kinases. (usda.gov)
  • Practical means to increase the dialytic clearance of bound solutes are required to perform such studies.METHODS: Artificial plasma was dialyzed using two dialysis systems in series. (stanford.edu)
  • To this end, more than 1200 samples were analyzed using the state-of-the-art mass spectrometry techniques and advanced bioinformatic methods. (tum.de)
  • In affected indivi- chemicals possessing the ability to react with duals, it has a serious impact on their quality of proteins, and hence these methods do not esti- life. (cdc.gov)
  • Most testosterone circulating in the blood is bound to sex hormone binding globulin (SHBG) and albumin, rendering only a small free (unbound) fraction (~1% to 2%) [ 1 ] able to diffuse across capillaries into target tissues, where it exerts biological activity. (medscape.com)
  • A protein complex network of Drosophila melanogaster . (nature.com)
  • Both the replication of the virus and the reaction of the cells are the result of complex protein signaling cascades. (tum.de)
  • In line with a more complex picture, multiple isoforms of the protein likely exist and non-centrosomal sites of localisation have been reported. (springer.com)
  • In addition to the host regulatory mechanisms response to disease, the microbiome can make changes to the expression of the host features such as their genes, proteins, and/or PTMs. (rsc.org)
  • Subsequently, FUS has also emerged as a significant disease protein in a subgroup of frontotemporal dementias (FTDs), previously characterized by immunoreactivity of the inclusion bodies for ubiquitin, but not for TDP-43 or tau with a proportion of the inclusions also containing alpha-internexin (α-internexin) in a further subgroup known as neuronal intermediate filament inclusion disease (NIFID). (wikipedia.org)
  • To this end, we identified the protein sequences of all four antibodies that binds to B7-H3 protein. (lls.org)
  • The purified protein is well folded and composed of oligomers with a varying number of subunits according to small-angle X-ray scattering (SAXS). (lu.se)
  • 2DE analysis of alkaline proteins is challenging due to the difficult separation of basic proteins during the isoelectric focusing (IEF). (nih.gov)
  • Many proteins with ill-defined functions were identified as well as a number of proteins that at the time of the analysis were described only as ORFs. (lu.se)
  • FUS/TLS was initially identified as a fusion protein (FUS-CHOP) produced as a result of chromosomal translocations in human cancers, especially liposarcomas. (wikipedia.org)
  • The doctoral student did this by recreating 258 flexible protein regions in test tubes - both "healthy" variants as well as disease-related ones - and then adding human cell extracts. (eurekalert.org)
  • Some studies have suggested a role for this protein in maintaining centriole-nucleated sensory organelles termed primary cilia, and AS is now considered to belong to the growing class of human genetic disorders linked to ciliary dysfunction (ciliopathies). (springer.com)
  • No studies however have tested testing whether increasing the clearance of bound solutes provides clinical benefit. (stanford.edu)
  • By using this method, MS identification of the target protein and several dozen interacting proteins is possible using a 100-min LC-MS/MS run. (nature.com)
  • SQR consists of a membrane-peripheral heterodimer domain (often called succinate dehydrogenase) which is tightly bound to a membrane-spanning anchor, which is a cytochrome with one or two heme groups depending on the organism. (lu.se)