• Right now, hematopoietic stem cell transplantation (HSCT) is the foremost dependable healing treatment with amazing comes about for patients with HLAmatched family or disconnected givers. (alliedacademies.org)
  • The WASP quality has 12 exons and encodes a 502 amino-acid protein (WASP), which is overwhelmingly communicated in nonerythroid hematopoietic cells [ 3 ]. (alliedacademies.org)
  • Aged hematopoietic stem cells (HSC) exhibit compromised reconstitution capacity and differentiation-bias towards myeloid lineage, however, the molecular mechanism behind it remains not fully understood. (haematologica.org)
  • In this study, we observed that the expression of pseudouridine (Ψ) synthase 10 is increased in aged hematopoietic stem and progenitor cells (HSPC) and enforced protein of Ψ synthase 10 (PUS10) recapitulates the phenotype of aged HSC, which is not achieved by its Ψ synthase activity. (haematologica.org)
  • Hematopoietic stem cells (HSC) generate all of the blood cells throughout their life-span. (haematologica.org)
  • This phase I trial assessed the safety, efficacy, and immunologic responses to minor histocompatibility antigens following nonmyeloablative allogeneic hematopoietic cell transplantation as treatment for metastatic renal cell carcinoma. (aacrjournals.org)
  • Eight patients received conditioning with fludarabine and low-dose total body irradiation followed by hematopoietic cell transplantation from an HLA-matched sibling donor. (aacrjournals.org)
  • Treatment of metastatic renal cell carcinoma with allogeneic hematopoietic cell transplantation after nonmyeloablative conditioning with fludarabine/total body irradiation is feasible and may induce tumor regression or stabilization in some patients. (aacrjournals.org)
  • FLT3 ITD triggers the proliferation of the quiescent hematopoietic stem cell (HSC) pool but fails to directly transform HSCs. (biomedcentral.com)
  • Though untreated the disease is uniformly fatal, newer advances in treatment like autologous hematopoietic stem cell transplantation, mini-transplants and advances in chemotherapy have improved the quality of life and increased survival (Gupta et al. (journalcra.com)
  • Donor cell-derived leukaemia (DCL) is an uncommon complication of allogeneic hematopoietic stem cell transplantation (HSCT). (lidsen.com)
  • Paroxysmal nocturnal hemoglobinuria or PNH is a rare benign clonal acquired hematopoietic stem-cell (HSC) disorder that results from somatic mutation of the X- linked phosphatidylinositol glycan class A gene known as the PIGA gene. (aacc.org)
  • Hematopoietic cells contain more than a dozen different GPI-anchored proteins including adhesion molecules, enzymes, and receptors. (aacc.org)
  • The mutant hematopoietic stem-cell exhibit a survival advantage over normal cells and tend to expand leading to hemolysis. (aacc.org)
  • Paroxysmal nocturnal hemoglobinuria (PNH) is a clonal hematopoietic stem cell (HSC) disease. (medscape.com)
  • Allogeneic hematopoietic stem cell transplantation is curative but it requires a histocompatible donor and is associated with significant morbidity and mortality, so it is reserved for severe cases of PNH with aplastic anemia or transformation to leukemia. (medscape.com)
  • The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
  • Currently, the efficiency for nuclear transfer is between 0-10%, i.e., 0-10 live births after transfer of 100 cloned embryos. (biomedcentral.com)
  • Photographs of (a) the procedure of somatic cell transfer (shown with the arrow) into the perivitelline space of an enucleated oocyte, (b) cloned bovine embryos used for transplantation to recipient animals, and (c) cloned calf (obtained in Russia for the first time). (isaaa.org)
  • Stem cells may be derived from adult tissues but the most potent are extracted from developing human embryos. (edu.au)
  • The researchers say the finding, which has been successfully demonstrated in frog embryos, will help scientists control the differentiation of various cell types. (scitechdaily.com)
  • When Notum was not present, the embryos would become a sack of skin cells with no head and a tiny brain, a result of embryonic progenitor cells making only epidermal but not neural cells. (scitechdaily.com)
  • Some prohibit only cloning for reproductive purposes and allow the creation of cloned human embryos for research, whereas others prohibit the creation of cloned embryos for any purpose. (who.int)
  • A targeted neuroglial reporter line generated by homologous recombination in human embryonic stem cells. (ca.gov)
  • In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
  • They further created a sheep called Polly in which they showed that it was possible to incorporate a human gene into the donor's DNA before cloning, thus indicating that it may be possible to use animals to produce human proteins for the benefit of mankind. (shawprize.org)
  • It is part of the saponin-like protein family, and its gene is found on the 2nd chromosome in humans. (wikipedia.org)
  • GNLY gene is located on human chromosome 2 and has 5 exons, which code for a 15 kDa protein. (wikipedia.org)
  • As a first step, the Nph3 gene was cloned and its exon-intron structure was mapped. (uni-goettingen.de)
  • In a related experiment, the research team was able to knock out the genes responsible for beta-lactoglobulin, a protein causing milk allergy in humans, in the hopes of creating gene-edited cows with hypoallergenic milk. (isaaa.org)
  • The molecular cloning of GCSF gene (Nagata et al. (allindianpatents.com)
  • One way to produce recombinant human G-CSF in soluble form is to produce chimeric protein formed by expression of a hybrid gene made by splicing together two gene sequences, In this approach, ideally GCSF would be operationaffy finked to an affinity tag so as to give chimeric protein with better solubility. (allindianpatents.com)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
  • The isolation of temperature-sensitive transformation-defective mutants of Rous sarcoma virus led to the hypothesis that the viral protein or proteins should be temperature-sensitive in its expression or function, and Dr. John Wyke and Rohrschneider found that expression of some of the cell-surface tumor antigens correlated with transformation, suggesting that they were under control of the transforming gene. (fredhutch.org)
  • The product of the PIGA gene is required for the synthesis of anchor protein known as GPI- anchor that ties other proteins to the cell surface. (aacc.org)
  • These PDXs display invasive growth, spontaneous metastasis, and recapitulate the histology, protein marker expression, and clinically important gene copy number changes of the original tumors. (lu.se)
  • Attempts were then made to show that mammalian cells - and human cells in particular - could also be reprogrammed back to a pluripotent state, because it is believed that such knowledge may advance our understanding of developmental mechanisms, and yield new approaches for disease treatment. (shawprize.org)
  • The 9 kDa form is also inhibited by cholesterol which is present in usually present in mammalian cells, but not in most pathogen cells. (wikipedia.org)
  • According to the type of mutation, deficiency of GPI-anchored protein can be partial or complete. (aacc.org)
  • thus, PIGA mutations lead to a deficiency of GPI-anchored proteins, such as complement decay-accelerating factor (also known as CD55) and CD59 glycoprotein (CD59), which are both complement inhibitors. (medscape.com)
  • Examples of projects this cohort include determining the effects of muscle progenitor cells on white blood cells function in mice with diet-induced obesity, evaluating migration of mesenchymal stem cells into biomaterials, and studying whole-body regeneration in a marine invertebrate, among others. (ca.gov)
  • It stimulates the survival, proliferation, differentiation and function of neutrophil granulocyte progenitor cells and mature neutrophils. (allindianpatents.com)
  • For those trying to create neural cells, "Notum is a necessary ingredient and new tool in the kit box for researchers to instruct human progenitor cells to become neural tissues," said He, who is also an American Cancer Society research professor. (scitechdaily.com)
  • Beyond this scientific interest, the commercial concern in animal cloning focuses on replicating large numbers of genetically identical animals, especially those derived from a progenitor that has been modified genetically. (who.int)
  • Recent studies have isolated distinct cell populations from infantile hemangiomas that display properties equivalent to aberrant progenitor cells, suggesting that, in addition to malignant tumors, benign tumors may also contain a stem cell-like component. (medscape.com)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • Fig. 4and encodes a glutamate receptor involved JNJ-26481585 in growth proliferation and survival of glioma and melanoma cells (23). (molecularcircuit.com)
  • Introduction: Multiple myeloma is characterized by proliferation of a clone of plasma cells that manifest by one or more lytic lesions, monoclonal (M) protein in the blood or urine and bone marrow involvement, ( Sirohi and Powles, 2004) having varying presentation and hematological features. (journalcra.com)
  • describes a novel colony stimulating factor (CSF) that has the ability to promote the differentiation and proliferation of human bone marrow cells to neutrophils, and a method to produce the same from a novel cell line which has been established from tumor cells in patients with oral cancer, The most published studies have used filgrastim as it was the first form of G-CSF to be approved. (allindianpatents.com)
  • Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
  • SCARA5 promoted cell proliferation and migration but inhibited apoptosis in ESCC. (hindawi.com)
  • SCARA5 expression is significantly downregulated in various tumor tissues including liver cancer (LC), rectal cancer, and glioblastoma [ 15 , 16 ] and might affect cell proliferation and invasion abilities by the focal adhesion kinase (FAK) signaling pathway and epithelial-mesenchymal transition (EMT) [ 5 , 17 - 20 ]. (hindawi.com)
  • Additionally, GEM or GEM+CDDP at low concentrations dramatically facilitated the proliferation, migration, and invasion of tumor cells in vitro . (researchsquare.com)
  • blood transfusion), spinal cord, lung cancer (oncology), respiratory cancer, radiology Background Multiple myeloma is a malignant proliferation of plasma cells mostly affecting the bone marrow and commonly presents with anaemia, hypercalcaemia, renal dysfunction, bone and skeletal involvement. (2011globalhealth.org)
  • First, we are interested in Epstein Barr Virus-mediated mechanisms of immune evasion with particular emphasis on pathways that promote survival and proliferation of EBV B cell lymphomas, the characterization of the human T cell and NK cell response to EBV and the identification of novel therapeutics for treatment of EBV B cell lymphomas. (stanford.edu)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • Patients were monitored for donor engraftment of myeloid and lymphoid cells, for clinical response by serial imaging, and for immunologic response by in vitro isolation of donor-derived CD8 + CTLs recognizing recipient minor histocompatibility (H) antigens. (aacrjournals.org)
  • However G-CSF in E. coli is expressed as inclusion bodies in the form of insoluble protein, which necessitates the additional steps for the isolation and purification of GCSF. (allindianpatents.com)
  • Isolation of an Extensively Drug-Resistant Pseudomonas aeruginosa exoS/O4 Strain Belonging to the 'High-Risk' Clone ST654 and Coproducer of NDM-1 and the Novel VIM-80. (cdc.gov)
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
  • 2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
  • Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
  • Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
  • Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
  • Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
  • BrainStorm Cell Therapeutics), uses mesenchymal stem cells harvested from patients' own bone marrow that are engineered to secrete neurotrophic factors, proteins important for the survival and function of neurons. (medscape.com)
  • Anti-Neurofilament NF-H Antibody, clone TA51 is an antibody against Neurofilament NF-H for use in ELISA, IP, WB, IH. (sigmaaldrich.com)
  • The cells stained positive with PAb416, a monoclonal antibody against the SV40 large T antigen. (cdc.gov)
  • After generation of hybridomas, we identified clones producing antibody against WU-VP1 by ELISA and immunoblot. (cdc.gov)
  • Since then, the work of Wilmut and Campbell has been duplicated in many other animal species and has provided approaches to produce useful therapeutic products with cloned animals and to improve agricultural practices. (shawprize.org)
  • The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • Solid organ transplantation is an important therapeutic option for children with a variety of end stage diseases. (stanford.edu)
  • Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)
  • They pioneered a new technique of starving embryo cells before transferring their nucleus to fertilized egg cells. (shawprize.org)
  • One of the live-born lambs, Dolly, was derived from the transplantation of the nucleus of an adult mammary cell. (shawprize.org)
  • The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)
  • However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
  • Thus, the clone would be genetically identical to the nucleus donor only if the egg came from the same donor or from her maternal line. (who.int)
  • Altered responsiveness to co- oup the nucleus accumbens reflect the behavioral approaches that incorporate caine and increased immobility in the forced swim test associated with elevated cAMP response ele- memory of associations between the extinction-like processes may have effi- ment binding protein in nucleus accumbens. (lu.se)
  • Through the cloning of Dolly, we learned that the cell nucleus contains all the genetic information needed for the cell to develop into any type of cell. (lu.se)
  • 1998). In parallel to the in vivo approach, a bacterial protein expression system was established, leading to the production of recombinant Nph1 and Nph3 for use in future in vitro experiments.Analysis of Nph3 expression in Nph3-KI mice was performed by assaying brain sections for beta-galactosidase activity, based on the co-expression of beta-galactosidase from Nph3-expressing cells. (uni-goettingen.de)
  • This method revealed the Nph3 expression pattern with high accuracy and sensitivity, suggesting a novel approach for the detailed in vivo identification of cells that express a particular protein. (uni-goettingen.de)
  • This study observes a previously neglected pharmacological phenomenon and investigates its mechanism of that the continuous low-dose administration of some antineoplastic agents in certain dose ranges can promote tumorigenesis and tumor progression in vitro and in vivo , through stimulation of tumor cell functions directly as well as enhancement of tumor angiogenesis by BMDCs recruitment indirectly. (researchsquare.com)
  • We combine next generation sequencing, in vitro drug testing , cell- and molecular biology techniques, in vivo drug testing and advanced in vivo imaging to understand and target metastatic and treatment resistant tumors. (lu.se)
  • We forecast that clone-specific practical profiling of GBMs can help determine intense clones new tumor driver systems molecular signatures and restorative vulnerabilities emphasizing the potential of tumor treatment at a clone-specific level. (molecularcircuit.com)
  • The path to transcription has not been elucidated: transcription factors, promoter regions, and pathogen-associated molecular patterns, which likely induce the signaling pathway necessary for the eventual translation of this protein, are unknown. (wikipedia.org)
  • 1993). Previous studies have shown that neurexophilins are secreted proteins of small molecular weight with structural features that resemble those of neuropeptides, suggesting a possible signaling function mediated by alpha-neurexins (Petrenko et al. (uni-goettingen.de)
  • Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. (lookformedical.com)
  • Detailed Description of Training Program Coursework: Our core coursework (i.e. courses taken by students from all three majors) is laboratory intensive, and includes Tissue Engineering, Cell Transplantation, Biomedical Imaging, and Molecular Techniques. (ca.gov)
  • Therefore, it is important to explore candidate genes that could have significance to the clinical diagnosis, as well as the treatment of esophageal squamous cell carcinoma (ESCC) at the molecular level [ 4 ]. (hindawi.com)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • She hails from Poland where she did her undergraduate work but has been at UCSF for over 24 years in Dr. Walter L Miller's group where she had a lab management role and was providing molecular and cell biology support for research in human steroid biosynthesis. (ucsf.edu)
  • Clones from three patients with a partial response or stable disease recognized antigens expressed on renal cell carcinoma tumor cells. (aacrjournals.org)
  • CD8 + CTL-recognizing minor H antigens on tumor cells can be isolated posttransplant and could contribute to the graft- versus -tumor effect. (aacrjournals.org)
  • A lesion with cytological characteristics associated with invasive carcinoma but the tumor cells are confined to the epithelium of origin, without invasion of the basement membrane. (lookformedical.com)
  • A cell line derived from cultured tumor cells. (lookformedical.com)
  • Cell-cycle arrest, activation of associated apoptotic proteins, and inhibition of apoptosis were also observed in tumor cells. (researchsquare.com)
  • Stem cell transplantation may lead to recovery of function through different mechanisms, such as the replacement of cells through differentiation, angiogenesis stimulation and support for micro environment. (copgfp.com)
  • Adult hair follicle bulge-derived stem cells (HFBSCs) has a capacity of neuronal differentiation, it is easy to harvest and relatively immune-privileged, which makes them potential candidates for autologous stem cell-based therapies. (copgfp.com)
  • More precisely, 15 kDa GNLY is capable of initiating differentiation of monocytes into dendritic cells. (wikipedia.org)
  • The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
  • Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
  • Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
  • These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
  • Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
  • culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
  • Immunologic variations from the norm of WAS incorporate T-cell lymphopenia, flawed proliferative reaction to CD3 cross-linking, disabled counter acting agent reaction to polysaccharide antigens, flawed monocyte chemotaxis, anomalies of fortified dendritic cells, and an expanded lymphocyte apoptosis with age. (alliedacademies.org)
  • The 15 kDa form is also able to act as a chemoattractant for different cells, such as NK cells, cytotoxic T cells, helper T cells, and in higher concentrations, immature dendritic cells. (wikipedia.org)
  • IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). (thermofisher.com)
  • Pereira's research also involves reprogramming skin cells into dendritic cells, which are the sentinels of the immune system. (lu.se)
  • In cancer patients dendritic cells may become dysfunctional or excluded from tumors. (lu.se)
  • Therefore, new ways are needed to reprogram other cells into immune cells with the same capacity as the "natural" dendritic cells. (lu.se)
  • By using skin cells as the starting cell-type, he managed to identify the three molecules needed to "program" dendritic cells in just nine days. (lu.se)
  • In the next step, he used the same molecules to reprogram cancer cells into dendritic cells. (lu.se)
  • and have been shown to induce vasculature JNJ-26481585 in the central nervous system (26 27 Interestingly the TMZ-sensitive clone shows increased expression of several genes involved JNJ-26481585 in neurotransmitter signaling such as glutamate receptors (did not correlate with TMZ responsiveness suggesting that new biomarkers of drug responsiveness are sorely needed consistent with more recent bulk GBM genomic analyses which highlight the subgroup limitations of this marker (1). (molecularcircuit.com)
  • utilizes a combination of high-resolution comparative genomic hybridization, short hairpin RNA inhibition of target genes at the locations of focal genomic deletions, and a primed cell mosaic mouse model to identify novel tumor suppressors in hepatocellular carcinoma. (biomedcentral.com)
  • Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
  • Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
  • The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
  • Targeted clone R-Olig2 (like the other clones) retained pluripotency, typical hESC morphology, and a normal parental karyotype 46,XY. (ca.gov)
  • Half a century ago, it was found by John Gurdon that this developmental clock can be reversed, and that differentiated somatic cells in a frog model could regain their pluripotency or totipotency. (shawprize.org)
  • These findings provide supporting evidence that enrichment for proteins involved in pluripotency is not restricted solely to malignant tumors as is suggested by the "stem cell theory of cancer", but additionally extends to common benign vascular tumors such as hemangiomas. (medscape.com)
  • During the development of vertebrates, including humans, the fertilized egg develops into the embryo, and the cells in the embryo then proceed to differentiate to form somatic cells of different tissues and organs. (shawprize.org)
  • The fertilized egg is considered totipotent, as it can develop into a whole organism, while the cells in the embryo are pluripotent because they are capable of differentiating into somatic cells that make up all the organs. (shawprize.org)
  • These findings could benefit stem cell researchers trying to create specific tissue types or organs in the lab. (scitechdaily.com)
  • Xenotransplantation involves the transplantation of nonhuman tissues or organs into human recipients. (medscape.com)
  • In light of the lack of supply of human organs for transplantation, several alternatives have been investigated and debated. (medscape.com)
  • Additionally, organs from animal sources could be transplanted into patients currently excluded from the human organ transplantation list. (medscape.com)
  • Finally, most patients perceive xenotransplantation as an acceptable bridge to transplantation of human organs in life-threatening situations. (medscape.com)
  • Cells are collected from donor (a) and cultured in vitro (b). (biomedcentral.com)
  • A matured oocyte (c) is then enucleated (d) and a donor cell is transferred into the enucleated oocyte (e). (biomedcentral.com)
  • Various strategies have been employed to modify donor cells and the nuclear transfer procedure in attempts to improve the efficiency of nuclear transfer. (biomedcentral.com)
  • Most of these efforts are focused on donor cells. (biomedcentral.com)
  • if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
  • They developed the buttonhole technique for anastomosis of donor and recipient vessels in kidney transplantation to prevent thrombus formation. (medscape.com)
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • 235.4: Analyzing the Impact of T-Cell Receptor Diversity in Acute Kidney Transplant Rejection. (ucsf.edu)
  • 347.2: Multiplexed Droplet Single-Cell Sequencing (Mux-Seq) of Normal and Transplant Kidney. (ucsf.edu)
  • Neurexophilins (Nphs) form a family of proteins that were discovered as components of the alpha-latrotoxin receptor, based on their strong binding to the synaptic membrane proteins alpha-neurexins (Petrenko et al. (uni-goettingen.de)
  • Additionally, the researchers were able to demonstrate how Notum deactivates Wnt, which is a family of proteins that direct stem cells to "self-renew," or make more stem cells, among other things. (scitechdaily.com)
  • [ 2 ] The amyloid of primary systemic amyloidosis is made by plasma cells in the bone marrow. (medscape.com)
  • G-CSF can be used to increase the numbers of circulating haemopoietic precursor cells which are then harvested by leucapheresis and used either as a supplement to or a substitute for aspirated bone marrow cells in autologous haemopoietic cell transplants. (allindianpatents.com)
  • The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
  • Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
  • Myelodysplastic syndrome refers to a group of related disorders in which abnormal blood-forming cells develop in the bone marrow. (msdmanuals.com)
  • White blood cells develop from stem cells in the bone marrow. (msdmanuals.com)
  • In myelodysplastic syndromes, a line of identical cells (clone) develops and occupies the bone marrow. (msdmanuals.com)
  • Bone Marrow Examination Red blood cells, most white blood cells, and platelets are produced in the bone marrow, the soft fatty tissue inside bone cavities. (msdmanuals.com)
  • Tumor and endothelial cell functions, apoptosis, cell cycle analysis, as well as bone marrow derived cells (BMDCs) mobilization, were evaluated with transwell, MTT or flow cytometry analysis in vitro , respectively. (researchsquare.com)
  • Solitary extramedullary plasmacytoma (SEP) describes solid plasma cell tumours of soft-tissue origin as opposed to solitary plasmacytoma of bone. (2011globalhealth.org)
  • 15]. The percentage of bone common of which are trisomy 8, mono- marrow blast cells for estimation of the Myelodysplastic syndrome (MDS) is somy 7 and 5q- [11]. (who.int)
  • A malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. (lookformedical.com)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
  • High-dimensional profiling of pediatric immune responses to solid organ transplantation. (stanford.edu)
  • The motivation for using animal sources for organ or tissue transplantation is driven by supply and demand. (medscape.com)
  • According to the most current report from the United Network for Organ Sharing (UNOS), more than 107,241 Americans were waiting for organ transplantation as of May 2010. (medscape.com)
  • Alexis Carrel is known as the founding father of experimental organ transplantation because of his pioneering work with vascular techniques. (medscape.com)
  • Clonal protein manifestation of EGFRvIII was examined with Traditional western blots using EGFRvIII-transfected human being fetal mind cells. (molecularcircuit.com)
  • In addition, high expression of Granulysin can be found in the placenta to protect fetal epithelial cells. (wikipedia.org)
  • The middle nucleated cell dosage from the marrow was 4.55 × 109/ kg (run, .3 to 7.9). (alliedacademies.org)
  • The blastocyst can then be transferred to a recipient (h) and cloned animals are born after completion of gestation (i). (biomedcentral.com)
  • We detected WU polyomavirus (WUPyV) in a bronchoalveolar lavage sample from lungs transplanted into a recipient with Job syndrome by using immunoassays specific for the WUPyV viral protein 1. (cdc.gov)
  • We report WUPyV cell tropism in lungs transplanted into a recipient with Job syndrome. (cdc.gov)
  • The expression of the epithelial cell adhesion molecule (EpCAM) is correlated with a poor prognosis, and treatment failure early tumor recurrence of hepatocellular carcinoma (HCC) patients. (copgfp.com)
  • Co-staining for an epithelial cell marker identified most WUPyV viral protein 1-positive cells as respiratory epithelial cells. (cdc.gov)
  • Spana and Doe, 1996 ) (mediated by Notch and Delta and cell-cell interaction), are involved in the specification of daughter cell fates. (biologists.com)
  • Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)
  • In this study, we targeted Olig2, a basic helix-loop-helix transcription factor that plays an important role in motoneuron and oligodendrocyte development, in human embryonic stem cell (hESC) line BG01 by homologous recombination. (ca.gov)
  • Thus, Dolly was the first example of the reprogramming of the adult cell back to totipotency in a mammal. (shawprize.org)
  • Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
  • He was in high school when he heard about Dolly, one of the world's most extreme examples of cell reprogramming. (lu.se)
  • Patients with coordinated kin or parent benefactors (MSD) and coordinated irrelevant benefactors (URD) display the most noteworthy survival rates up to 80%, particularly in case transplantation happens at an early age with a URD. (alliedacademies.org)
  • This first identification of an oncoprotein interacting with adhesion sites had a major impact on the field, since it provided an explanation for how Src and other oncoproteins could have such dramatic effects on cell morphology. (fredhutch.org)
  • Indeed, in less than a year, proteins interacting with Src in adhesion plaques were found to contain phosphotyrosine, a signature of Src activity. (fredhutch.org)
  • The graduates of this program have strengthened the future of stem cell research in California by contributing to all aspects of the field, both directly and in-directly. (ca.gov)
  • Graduates of our program are directly strengthening stem cell research and regenerative medicine by performing fundamental investigations in both academic laboratories (as research associates and doctoral students) and for-profit companies, developing and manufacturing regenerative medicine products at biotech companies, and participating in clinical-trial organization. (ca.gov)
  • Through this coursework, students learn both the theoretical knowledge and practical skills that are important for stem cell research/regenerative medicine and gain important preparation for their internship projects. (ca.gov)
  • Experts from around the world are assessing the difficult issue of the extent to which embryonic stem cell research should be allowed to proceed, and to date there is little international consensus on this matter. (edu.au)
  • How, then, should embryonic stem cell research be regulated in Australia? (edu.au)
  • This issue was considered by the House of Representatives Standing Committee on Legal and Constitutional Affairs in its report entitled Human Cloning: Scientific, Ethical and Regulatory Aspects of Human Cloning and Stem Cell Research (hereafter the Andrews Report , after the Chair of the Committee, Mr Kevin Andrews, MP) released in September 2001. (edu.au)
  • In this article we examine embryonic stem cell research and explore the current regulatory framework associated with this research in Australia, with particular reference to the Andrews Report . (edu.au)
  • Cells also expressed laminin and fibronectin extracellular matrix but the mass was not detected. (copgfp.com)
  • The Ly-49 family of NK-cell receptors are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains (CRD) that bind to MHC class I alloantigens. (bdbiosciences.com)
  • 13-15 Ψ plays an important role in various aspects of RNA biology, and, therefore, participates in many biological process, including translational control, 16 , 17 RNA folding, 18-22 protein translation, 23-26 and clinical diseases. (haematologica.org)
  • Terms such as AL describe the protein (light chain), but do not necessarily describe the clinical phenotype. (medscape.com)
  • Diet-induced compromised microenvironments fatty liver was established to mimic the clinical and patient non-alcoholic steatohepatitis (NASH) and the ability of tumorigenic cells Hepa1-6 evaluated. (copgfp.com)
  • A 28-year-old woman with Job syndrome was seen at the NIH Clinical Center 6 months after bilateral lung transplantation. (cdc.gov)
  • We predict that further studies of larger groups of patient tumors and derived clones are likely to yield additional clonal vulnerabilities that will have clinical relevance. (molecularcircuit.com)
  • It represents a large group of epithelial lung malignancies which can be divided into two clinical groups: SMALL CELL LUNG CANCER and NON-SMALL-CELL LUNG CARCINOMA . (lookformedical.com)
  • Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
  • Areas related to clinical and experimental transplantation are also of interest. (lidsen.com)
  • Clinical manifestations of PNH occur when a HSC clone carrying somatic PIGA mutations acquires a growth advantage and differentiates, generating mature blood cells that are deficient of GPI-anchored proteins. (medscape.com)
  • The name of the disorder is a descriptive term for the clinical consequence of red blood cell (RBC) breakdown with release of hemoglobin into the urine, which manifests most prominently as dark-colored urine in the morning (see image below). (medscape.com)
  • The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
  • Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. (biomedcentral.com)
  • Two GPI-anchored proteins (CD55&CD59) normally function as complement regulatory proteins. (aacc.org)
  • We developed an immunohistochemical (IHC) assay to detect the WUPyV viral protein 1 (WU-VP1) by using an IgG2b designated NN-Ab06. (cdc.gov)
  • In order to guide or direct stem cells to differentiate into a given cell type, such as neural cells or muscle cells, researchers continue to alter their experimental recipes, fine-tuning which molecules should be added to their dishes in what sequence and amount. (scitechdaily.com)
  • They also performed heterotopic experimental transplantation. (medscape.com)
  • An experimental stem cell therapy for the treatment of mild to moderate amyotrophic lateral sclerosis (ALS) received a resounding rejection today from a US Food and Drug Administration (FDA) advisory panel that cited efficacy, safety, and product manufacturing concerns. (medscape.com)
  • All patients can evolve into trisomy 8+ MDS [12], cells, since acquiring adequate samples diagnosed with MDS have a reduced in which 8+ appears to confer a favour- for flow cytometric analysis renders the life expectancy compared with age- able prognosis [13]. (who.int)
  • Recombinant histidine-tagged WU-VP1 protein was generated by expressing WU-VP1 (GenBank accession no. (cdc.gov)
  • The present invention relates to a chimeric protein of recombinant human granulocyte colony stimulating factor comprising of hG-CSF fused to an affinity fusion tag at its amino terminus. (allindianpatents.com)
  • 1986, Science 232, 61-64) led to the production of recombinant protein and large scale manufacturing of the product. (allindianpatents.com)
  • Another form of recombinant human G-CSF called lenograstim is synthesised in Chinese hamster ovary (CHO) cells. (allindianpatents.com)
  • Full length human recombinant protein of human IDO1 produced in HEK293T cell. (thermofisher.com)
  • High-risk MDS cases had a significantly higher percentage of apoptotic CD34+ cells and anti-apoptotic survivin+ cells than controls, particularly for trisomy 8 cases. (who.int)
  • Trisomy 8+ cells showed a significant positive correlation with apoptotic CD34+ cells and capacity for colony formation. (who.int)
  • Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • The targeting vector was inserted into ES cells in culture and a Nph3-KI mouse line was generated (blastocyst injections and transplantations were performed in collaboration). (uni-goettingen.de)
  • Granzymes usually cause apoptosis of the infected cell through initiation of the caspase cascade. (wikipedia.org)
  • Rabbit polyclonal to Parp.Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-bindingzinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure,and apoptosis. (2011globalhealth.org)
  • The tissue suspensions were sonicated well and centrifuged in a TL-100 ultracentrifuge (Beckman Instruments, Fullerton, CA) at 100,000 g for 1 h at 25°C, and the protein concentrations in the supernatants were determined. (sigmaaldrich.com)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • Using improved immunofluorescence microscopy, Rohrschneider soon realized that most of the Src oncoprotein is actually concentrated in small regions of the cell membrane where the internal cytoskeleton sticks to the external matrix that makes up the glue of connective tissue. (fredhutch.org)
  • Antigenicity for each protein was quantified based on staining intensity and percentage of tissue positive for each antigen, and subsequently compared to data obtained from two control tissue sets: 10 vascular tissues and a panel of 58 various malignant sarcomas. (medscape.com)
  • Semi-quantitative evaluation of our immunohistochemical staining revealed that protein expression of Oct4, Nanog, Myc, and Sox2, but not Klf4, was significantly increased in benign, borderline, and malignant vascular tumors relative to non-diseased vascular tissue controls. (medscape.com)
  • Biallelic hypomorphic variants in PPA2 , encoding the mitochondrial inorganic pyrophosphatase 2 protein, have been recently identified in individuals presenting with sudden cardiac death, occasionally triggered by alcohol intake or a viral infection. (nature.com)
  • Implantable mechanical devices have been explored in the field of cardiac transplantation. (medscape.com)
  • It is expressed in 2 forms: a 15kDa precursor protein, the translation product, and a 9kDa cytotoxic protein, which is formed after cleavage of the amino and carbonyl ends of the 15kDa protein. (wikipedia.org)
  • We find that NFATC1 governs FLT3 ITD -driven precursor cell expansion and transformation, causing a fully penetrant lethal AML. (biomedcentral.com)
  • These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. (biomedcentral.com)
  • Developmental defects, including abnormalities in cloned fetuses and placentas, in addition to high rates of pregnancy loss and neonatal death have been encountered by every research team studying somatic cloning. (biomedcentral.com)
  • Their study was given early online Thursday and is being published in print in the March 23 issue of the journal Developmental Cell . (scitechdaily.com)