Proteins shown to interact. Medical search. Frequent questions

Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. (degruyter.com)
The identification of the intracellular sGAG-interacting proteins could help to unravel these functions. (degruyter.com)
The addition of an affinity tag allowed us to avoid the use of antibodies targeted toward the intracellular C-terminal tail of APP, a region to which a number of reported interacting proteins bind ( King and Turner, 2004 ). (jneurosci.org)
In this study, we found that mouse Ahi1 formed a stable complex with huntingtin-associated protein 1 (Hap1), which is critical for neonatal development and involved in intracellular trafficking. (jci.org)
Mechanisms by which E. chaffeensis establishes intracellular infection, and avoids host defenses are not well understood, but involve functionally relevant host-pathogen interactions associated with tandem and ankyrin repeat effector proteins. (frontiersin.org)
Despite its small genome and limited number of effector proteins, Ehrlichia efficiently establishes an intracellular infection and avoids immune defenses in vertebrate and invertebrate hosts through complex molecular and cellular reprogramming strategies. (frontiersin.org)
Some family members, such as latrophilins and gliomedin, are membrane-bound proteins containing the olfactomedin domain in the extracellular N-terminal region, while the intracellular C-terminal domain of these proteins is essential for the transduction of extracellular signals to the intracellular signaling pathway. (nih.gov)
Anna Blom hopes that the new study will pave the way for new research that can increase our knowledge about the significance of intracellular proteins in the development of diabetes and other diseases. (lu.se)

In humans, the CRYAA gene encodes a 173 amino acid residue protein by single copy genes located on chromosome 21. (molvis.org)
In the current study, genes encoding two related proteins, PnMas1 and PnMas3, were identified in the genome of P. parasitica . (springer.com)
BLAST analyses showed that Mas genes only occur in flagellate species within the Stramenopile taxon. (springer.com)
both methods indicated suppression of genes and proteins participating in mitochondrial metabolism. (jci.org)
Our data show that the stable knockdown of HIPK2 led to wtp53 misfolding, as detected by p53 immunoprecipitation with conformation-specific antibodies, and that p53 protein misfolding impaired p53 DNA binding and transcription of target genes. (aacrjournals.org)
It is well known that in a biological system genes interact with each other forming various biological pathways in order to carry out a multitude of biological processes. (hindawi.com)
The genes obtained from this study showed a significant involvement in causing the disease, which may be used as a potential drug target. (researchgate.net)
Next, we will generate recombinant proteins using the cloned genes that have shown a measurable plant response. (usda.gov)
Once in the right place, the protein can begin putting copies of its genes into new infectious virus particles, invading more of our cells, and making us sicker. (news-medical.net)
We study genes, proteins and signaling pathways that might be essential for RGC and optic nerve development, function, survival, and regeneration. (nih.gov)
We are particularly interested in genes encoding olfactomedin domain-containing proteins. (nih.gov)
Several genes encoding olfactomedin domain-containing proteins are expressed in the eye. (nih.gov)
Olfactomedin 1 (Olfm1) and the closely related genes Olfactomedin 2 (Olfm2) and Olfactomedin 3 (Olfm3) show overlapping expression patterns demonstrating preferential expression in the developing and adult neuronal tissues. (nih.gov)
Here, we show that among 45 transcription factors up-regulated in mycorrhizal roots of the legume Lotus japonicus, expression of a unique GRAS protein particularly increases in arbuscule-containing cells under low phosphate conditions and displays a phylogenetic pattern characteristic of symbiotic genes. (uni-koeln.de)

We report here the identification and characterization of a novel FMRP-interacting protein associated to polyribosomes as a component of mRNP complexes containing FMRP. (unipv.it)
We named this protein 82-FIP (82-kD FMRP Interacting Protein). (unipv.it)
FMRP binds Cytoplasmic FMRP Interacting Protein (CYFIP). (fraxa.org)

Several large-scale efforts have systematically catalogued protein-protein interactions (PPIs) of a cell in a single environment. (nature.com)
Interactions with other relevant participants such as small molecules (purple), sub-complexes (yellow), and other subunits (red) are also shown. (yeastgenome.org)
To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray. (molvis.org)
A HuProt microarray composed of 17,225 human full-length proteins with N-terminal glutathione S-transferase (GST) tags was used to identify protein-protein interactions. (molvis.org)
The protein-protein interactions may help CRYAA carry out multifaceted functions. (molvis.org)
To understand the functions of CRYAA and how these are regulated, protein-protein interactions need to be investigated. (molvis.org)
The nucleoporins were tested for interactions with non-specific proteins and with chaperones, which are proteins that act as transport tickets through the pore. (rug.nl)
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). (hmdb.ca)
This method is more sensitive than other techniques and gives us a more comprehensive view of all the interactions between the human and viral proteins,' he said. (news-medical.net)
Interactions of HCMV with the cell cycle regulatory protein p53. (uidaho.edu)
Computational studies to elucidate how SWCNTs may interact with TLR4 in the absence of a protein corona suggested that binding is guided mainly by hydrophobic interactions. (cdc.gov)
Previous studies have shown that the pathogen infection of mosquitoes can alter reproductive fitness, revealing specific vector-pathogen interactions that are key determinants of vector competence. (bvsalud.org)
Viruses and people interact in many ways and sometimes those interactions cause us harm. (cdc.gov)
When combined with the right virological experiments, human genomic studies have the potential to show us the precise mechanisms involved in such interactions. (cdc.gov)

This gene encodes a protein that contains an N-terminal RING finger motif and a putative coiled-coil domain. (wikipedia.org)
Essential for recruitment of Atg proteins to the pre-autophagosomal structure, the putative site for autophagosome formation, under starvation condition, resulting in the sequestration of cytoplasmic proteins and organelles for bulk degradation. (yeastgenome.org)
A similar coiled-coil domain is present in several putative human proteins and in the mitotic phosphoprotein 43 ( MP43 ) of X. laevis. (xenbase.org)
Both isoform 3 of the human mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4) and Traf2- and Nck-interacting kinase (TNIK) are putative effectors of Rap2 in mediating the activation of c-Jun N-terminal kinase (JNK) to regulate the actin cytoskeleton. (nih.gov)
A total of 140 putative Ca2+-calmodulin-binding proteins were identified of which 87 proteins contained calmodulin-binding motifs. (lu.se)

The highly conserved NH(2)-terminal coiled-coil domain of the NUDE protein suffices for protein function when overexpressed. (xenbase.org)
The 14-3-3 proteins are highly conserved in all eukaryotes. (elifesciences.org)
Here, we demonstrate that an mRNA-lipid nanoparticle vaccine encoding a set of four highly conserved MPXV surface proteins involved in virus attachment, entry, and transmission can induce MPXV-specific immunity and heterologous protection against a lethal vaccinia virus (VACV) challenge. (bvsalud.org)

Here, we develop a highly parallel protein-protein interaction sequencing (PPiSeq) platform that uses a novel double barcoding system in conjunction with the dihydrofolate reductase protein-fragment complementation assay in Saccharomyces cerevisiae . (nature.com)
The interaction of sGAG with α2-macroglobulin receptor-associated protein (LRPAP1), exportin-1 (XPO1), and serine protease HTRA1 (HTRA1) was confirmed in reverse assays. (degruyter.com)
FMRP interacts with 82-FIP through a novel interaction motif located in its N-terminal region. (unipv.it)
PRDM9 is a member of the PRDM family of transcription regulators, but unlike other family members, it contains a Krüppel-associated box (KRAB)-related domain that is predicted to be a potential protein interaction domain. (springer.com)
In S. cerevisiae , this interaction is at least in part provided by Spp1 that directly interacts with both methylated H3K4 near DSB sites and the axis-localized protein Mer2 (Acquaviva et al. (springer.com)
Blocking the interaction between PIF7 and 14-3-3 proteins or reducing the expression of 14-3-3 proteins accelerates shade-induced nuclear localization and de-phosphorylation of PIF7, and enhances the shade phenotype. (elifesciences.org)
But with further study into how G-quadruplexes and the gene behind Cockayne Syndrome interact we can learn details that will hopefully allow us to discover therapeutic tools, such as designer molecules that can regulate the interaction and fight back against the premature ageing caused by the disease. (worldhealth.net)
In their work, the Unit of Structural Dynamics of Macromolecules presented the structure of the DNA-bound PolD-PCNA complex from P. abyssi at 3.77 Å using an integrative structural biology approach, combining cryo-EM, X-ray crystallography, protein-protein interaction measurements and activity assays. (pasteur.fr)
2023). Human Post-Translational SUMOylation Modification of SARS-CoV-2 Nucleocapsid Protein Enhances Its Interaction Affinity with Itself and Plays a Critical Role in Its Nuclear Translocation. (news-medical.net)
These results show that interaction between FGF13 and Na V 1.7 is required for itch-inducing substances to activate subpopulations of DRG neurons. (jneurosci.org)
Through interaction with these receptors, the SLURP-1 protein is thought to be involved in controlling the growth and division (proliferation), maturation (differentiation), and survival of skin cells. (medlineplus.gov)

AD is pathologically characterized by the deposition of pathogenic Aβ peptides that are derived from larger integral membrane proteins, termed β-amyloid precursor proteins (APPs). (jneurosci.org)
Using this tag, we prepared mild detergent lysates from transgenic mouse brain cortical membrane preparations and isolated a number of previously identified APP-interacting proteins. (jneurosci.org)
Aβ is liberated from type I integral membrane proteins, termed β-amyloid precursor proteins (APPs), by the concerted action of β-secretase (BACE1) and γ-secretase (for review, see Selkoe, 2002 ). (jneurosci.org)
A . Schematic of the bouquet configuration of chromosomes during meiotic prophase I. The spatial arrangement of chromosomes with telomeres clustered and attached at the inner nuclear membrane is shown. (biorxiv.org)
Using single-molecule tracking, we show that all three Y-complex proteins exhibit three distinct mobilities, including movement through the cytosol and confined motion, predominantly at membrane-proximal sites but also within the cell center. (uni-marburg.de)
Cell fractionation reveals that YaaT displays a mixture of membrane association and presence in the cytosol, while YlbF and YmcA do not show direct membrane attachment. (uni-marburg.de)
Taken together, our experiments reveal membrane-associated and membrane-independent activities of Y-complex proteins and a dynamic interplay between them with indirect membrane association of YmcA and YlbF via YaaT. (uni-marburg.de)
Protein crowding induces membrane curvatures through an entropic mechanism. (portlandpress.com)
Crowding of asymmetric proteins results in an asymmetric lateral pressure across the membrane which can be used by cells in a number of biological processes involving membrane remodeling. (portlandpress.com)
Lipid binding is essential for membrane attachment, a key feature of most Ras proteins. (nih.gov)
The team's new investigation showed how this is possible using computer modelling which showed that CD59 is very mobile on the membrane. (imperial.ac.uk)
I will present our recent structural studies, using solid-state NMR spectroscopy, of three membrane proteins that give insights into the mechanisms of virus-cell membrane fusion and virus budding. (lu.se)
1) We have investigated the structure of the membrane-interacting domains of the fusion proteins of the parainfluenza virus 5 (PIV5) and human immunodeficiency virus (HIV). (lu.se)
For the PIV5 fusion protein, the fusion peptide (FP) and the transmembrane domain (TMD) show striking membrane-dependent conformations. (lu.se)
2) For the HIV fusion protein gp41, we have determined the oligomeric structure of the membrane-proximal external region (MPER) and the TMD. (lu.se)
To understand how cholesterol interacts with M2 to generate membrane curvature, we have determined the cholesterol-binding site of M2 by measuring protein-cholesterol distances and cholesterol orientation in the membrane. (lu.se)
The data represent the first direct determination of the cholesterol-binding structure of a membrane protein in lipid bilayers, and moreover indicate a specific mechanism by which cholesterol concentration gradients in the membrane drive the M2 protein to the neck of the budding virus to conduct membrane scission. (lu.se)

Interestingly, it also acts as an adaptor in complexes that mediate autophagy of aggregated proteins. (unil.ch)
VCP identifies ubiquitylated substrates through numerous dedicated adaptor proteins and unfolds substrates by threading them through a central pore in the hexamer ( Figure 1 ). (portlandpress.com)
VCP interacts with adaptor proteins to identify ubiquitylated substrates for degradation by the proteasome. (portlandpress.com)
Chemokine secretion was reduced upon inhibition of NF-kappaB, as predicted by upstream regulator analysis of the transcriptomics data, and Toll-like receptors (TLRs) and their adaptor molecule, MyD88 were shown to be important for CCL5 secretion. (cdc.gov)

Anti-PnMas1 and anti-PnMas2 antibodies co-labelled two high-molecular-weight (~400 kDa) protein complexes in native gels but anti-PnMas3 antibodies labelled a 65 kDa protein complex. (springer.com)
The recombinant proteins will be used as antigens to produce polyclonal antibodies. (usda.gov)
The antibodies will be used for western blots or immunostaining to detect the presence of the Hessian fly proteins in infested wheat tissues. (usda.gov)
The same antibodies will be used for immunoprecipitation to identify interacting targets of Hessian fly family 31 effectors in wheat tissues. (usda.gov)
It achieves this by training immune cells to identify other cells that have been infected, and by producing and refining antibodies - proteins that bind and block the virus, so it doesn't infect more cells. (cdc.gov)
This could be due to antibodies that were made against some other immunogen but cross-reacted with Ebola virus, by innate immunity proteins in their blood, or both. (cdc.gov)

We describe the proteomic characterization of mouse brain Ca2+-calmodulin-binding proteins that were purified using calmodulin affinity chromatography. (lu.se)

Purification of APP-AT protein complexes and identification of the constituents by mass spectrometry revealed several previously reported APP-interacting proteins as well as proteins involved in synaptic maintenance. (jneurosci.org)
The molecules bound to recombinant family 31 proteins will be eluted and will be identified via liquid chromatography/mass spectrometry quadrupole time of flight (LC-Q-TOF) instrumentation. (usda.gov)
140 mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry. (lu.se)

These attachment sites are linked to actin-bundles that surround the nucleus via an Ndj1-Mps3-Csm4 protein bridge that spans the inner and outer nuclear membranes. (biorxiv.org)
Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. (hmdb.ca)
With a $163,000 grant from the FRAXA Research Foundation, Dr. Scott Soderling and Dr. Hwan Kim bred the standard mouse model of Fragile X syndrome to their lines of mice that express reduced levels of several key proteins that modulate synaptic actin. (fraxa.org)
Several of the mRNAs regulated by FMRP directly impact spine actin dynamics, including both the mRNA encoding the Rac GTPase, a key regulator of actin dynamics, and the mRNA encoding profilin, an actin binding protein. (fraxa.org)
Our previous work has shown that WAVE-1 is an important actin regulatory protein that regulates dendritic spines (a component of the synapse) and synaptic plasticity. (fraxa.org)
In human platelets, Rap2 was shown to interact with the cytoskeleton by binding the actin filaments. (nih.gov)

The cognate effector for Hdic is predicted to interact with important plant defense networks. (usda.gov)
A number of additional effector proteins for Rap2 have been identified, including the RalGEFs RalGDS, RGL, and Rlf, which also interact with Rap1 and Ras. (nih.gov)

DSB sites are preferentially located within chromatin loops, while several proteins that are required for DSB formation (Rec114, Mei4, and Mer2) localize on the chromosome axis (Blat et al. (springer.com)
We demonstrated that Olfm1 interacts with several proteins essential for neuronal and synaptic activity. (nih.gov)

Cell-fate-determinant molecule NUMB-interacting protein (TBC1D15) is overexpressed and contributes to p53 degradation in TICs. (nature.com)
The AAA-ATPase (ATPases associated with diverse cellular activities) valosin-containing protein (VCP), is essential for many cellular pathways including but not limited to endoplasmic reticulum-associated degradation (ERAD), DNA damage responses, and cell cycle regulation. (portlandpress.com)
VCP primarily identifies ubiquitylated proteins in these pathways and mediates their unfolding and degradation by the 26S proteasome. (portlandpress.com)

A His-tag will be added to each recombinant protein to facilitate protein purification. (usda.gov)

Homeodomain-interacting protein kinase-2 (HIPK2) is a positive regulator of p53 oncosuppressor function. (aacrjournals.org)
We recently showed that NANOG-mediated induction of Aurora A kinase (AURKA), an upstream kinase for aPKCζ, and repression of Lethal Giant Larva 2 (LGL2 or LLGL2), an aPKCζ inhibitor, leads to aPKCζ upregulation and activation. (nature.com)

The family of olfactomedin domain-containing proteins is one of the main areas of our research. (nih.gov)

The mutation lies near the binding site for most PCNA-interacting proteins. (rcsb.org)
We show that the mutation markedly alters the binding energetics for some client proteins, while another, p21(CIP1), is only mildly affected. (rcsb.org)
the p.Ser101Arg mutation abrogates the ability of B9d2 to interact with Mks1, which suggests that this mutation compromised B9d2 function. (medscape.com)
The bar plot below shows the proportion of tumor samples that have any kind of altering mutation(s) in the given protein. (phosphosite.org)
A mutation that inhibited binding of the protein to the A-T-rich region also aborted the transcriptional stimulatory effect of the region. (lu.se)

Bacterial proteins could alter precipitation patterns and climate-change models. (insidescience.org)
Now, new research shows how bacterial proteins interact with water molecules to form ice. (insidescience.org)
However, there is a downside to CD59, as it can also be hijacked by bacterial proteins to target human cells for invasion. (imperial.ac.uk)
Previous work by the team published in Cell Reports showed that the parts of CD59 that interact with bacterial proteins are the same parts responsible for stopping MAC. (imperial.ac.uk)
Bacterial proteins exploit this flexibility to co-opt CD59 for infection. (imperial.ac.uk)

Studies show that the SLURP-1 protein can attach (bind) to nicotinic acetylcholine receptors (nAChRs) in the skin. (medlineplus.gov)
Two Ets proteins, PU.1 and elf-1, that have previously been shown to bind to an adjacent DNA element were shown to physically interact with CArG- box binding factor.A. An antiserum raised against the protein recognized two different forms indicating either that different splice-forms of. (lu.se)
After comparing the results from the different experiments, we were able to exclude a significant number of proteins suspected to bind in a nonspecific manner. (lu.se)

The pre-replication complex protein, origin recognition complex-associated (ORCA/LRWD1), preferentially localizes to heterochromatic regions in post-replicated cells. (nih.gov)
Now, researchers from the Molecular Science Research Hub at Imperial College London have observed a protein called Cockayne Syndrome B (CSB) preferentially interacting with one specific type of G-quadruplex. (worldhealth.net)

This table lists all participants of the complex (proteins, small molecules, nucleic acids, etc.) and their respective stoichiometry. (yeastgenome.org)
Acyl-CoA binding protein (ACBP) maintains a pool of fatty acyl-CoA molecules in the cell and plays a role in fatty acid metabolism. (rcsb.org)
Non-interacting proteins or other molecules will be washed off with buffers. (usda.gov)
Then they blasted intense laser beams with short pulses of just 40 femtoseconds -- that's the amount of time it takes light to travel just one one-hundredth of a millimeter -- over the sample to examine the atomic motion of the protein and water molecules as they dropped the experiment from balmy room temperature to near-freezing. (insidescience.org)
Brent Christner, a microbiologist at Louisiana State University in Baton Rouge who also studies ice-nucleators, said that scientists still understand very little about the precise way in which these proteins interact with water molecules and limit supercooling -- the process by which water or another liquid can drop below its freezing point without turning solid. (insidescience.org)

PR domain-containing protein 9 (PRDM9) is a major regulator of the localization of meiotic recombination hotspots in the human and mouse genomes. (springer.com)
The data further show that B9d1 is required for normal Hedgehog (Hh) signaling, ciliogenesis, and ciliary protein localization. (medscape.com)
These studies have revealed that 14-3-3 proteins can interact with the phosphorylated forms of their client proteins in response to certain signals, and that this binding finalizes the signaling event by enabling a change in the subcellular localization, protein stability or intrinsic enzymatic activity of the client, or by promoting an association between the client and other proteins. (elifesciences.org)

Note: No diagram is shown ("No shared annotations") if there are less than 2 shared annotations (either GO terms or subunits of other complexes) between this complex and any other complexes. (yeastgenome.org)

Mutations in the Abelson helper integration site 1 gene, which encodes the protein AHI1, have been shown to cause Joubert syndrome. (jci.org)
Normally functioning CSB proteins do not cause any ill effects, but mutations of the gene that produce CSB protein can cause the fatal premature ageing disorder Cockayne Syndrome, which kills many sufferers before adulthood. (worldhealth.net)
The team found that CSB proteins with mutations that cause Cockayne Syndrome are no longer able to interact with the long-range G-quadruplexes. (worldhealth.net)
Mutations in the SLURP1 gene lead to little or no SLURP-1 protein in the body. (medlineplus.gov)
Studies showed that these mutations result in a loss of CD59 complement inhibitory functions. (lu.se)

Here, we describe the structure of the disease-causing variant, which reveals a large conformational change that dramatically transforms the binding pocket for PCNA client proteins. (rcsb.org)

Meiotic recombination is initiated by programmed DNA double-strand breaks (DSBs), generated by SPO11 and accessory proteins (de Massy 2013 ). (springer.com)
Across every domain of life, polymerase holoenzyme accessory proteins play an integral role in achieving the extraordinary efficacy and accuracy of the replicative polymerase complex. (pasteur.fr)

COVID takes advantage of a human post-translation process called SUMOylation, which directs the virus' N protein to the right location for packaging its genome after infecting human cells. (news-medical.net)
2) in those centers, p53 binds site-specifically to the viral genome and 3) infection of p53 knockout cells show dramatic decreases in viral titers, in viral DNA replication and in viral protein expression. (uidaho.edu)

To investigate the APP in vivo interactome in an unbiased manner, we generated mice that harbor a mouse prion protein promoter-driven cDNA encoding human APP-695 fused to a C-terminal affinity tag. (jneurosci.org)
A protein interacting with an A-T-rich region that is a positive control element within the SP6 κ promoter was purified and identified as CArG-box binding factor-A. The purified protein was shown to interact specifically with the coding strand of single-stranded DNA and, with lower affinity, with double-stranded DNA. (lu.se)
Proteins in the eluates from four different affinity chromatography experiments were identified by 1-DE and in-gel digestion followed by LC-MS/MS. Parallel experiments were performed using two related control-proteins belonging to the EF-hand family. (lu.se)

The LIS1 -related NUDF protein of Aspergillus nidulans interacts with the coiled-coil domain of the NUDE/RO11 protein. (xenbase.org)

Functional annotation clustering showed that they belong to cell cycle, organelle or nuclear lumen, protein transport, and DNA binding and repair clusters. (molvis.org)
The product of the nudE gene isolated in the screen, NUDE, is a homologue of the nuclear distribution protein RO11 of Neurospora crassa. (xenbase.org)
The nuclear pore complex is one of the biggest protein structures in the cell,' explains Patrick Onck, professor of Micromechanics at the University of Groningen. (rug.nl)
The eukaryotic DNA polymerase sliding clamp, proliferating cell nuclear antigen or PCNA, is a ring-shaped protein complex that surrounds DNA to act as a sliding platform for increasing processivity of cellular replicases and for coordinating various cellular pathways with DNA replication. (rcsb.org)
Here, we show that shade light induces the de-phosphorylation and nuclear accumulation of PIF7. (elifesciences.org)
The bacterial sliding clamp is referred to as the b clamp, while the eukaryotic and archaeal sliding clamp protein is called the proliferative cell nuclear antigen (PCNA). (pasteur.fr)

We will use changes in the gene expression levels of Mds-1 and the lipid transfer protein gene BT008922 as biomarkers to measure plant responses using RT-qPCR. (usda.gov)
Most Ras proteins contain a lipid modification site at the C-terminus, with a typical sequence motif CaaX, where a = an aliphatic amino acid and X = any amino acid. (nih.gov)

Valosin-containing protein (VCP, also p97, or Cdc48p in yeast) is an evolutionarily conserved, homo-hexameric, ubiquitin-selective, AAA-ATPase that functions in numerous ubiquitin-dependent protein quality control pathways. (portlandpress.com)

Thioredoxin-interacting protein (Txnip) is a regulator of metabolism and an inhibitor of the antioxidant thioredoxins, but little is known about its roles in the myocardium. (jci.org)

We need to know more on both the mechanism, in which the proteins cause freezing, as well as about their numbers and activity in the clouds. (insidescience.org)
Researchers show how a mechanism that human cells use to protect themselves against overzealous immune responses can be hijacked by invading bacteria. (imperial.ac.uk)

In Arabidopsis , the shade-induced de-phosphorylation of the transcription factor PIF7 (PHYTOCHROME-INTERACTING FACTOR 7) is the key event linking light perception to stem elongation. (elifesciences.org)

Proteomics is the study of all the proteins that an organism makes, how they are modified by other enzymes, and the roles they play in a living organism. (news-medical.net)

Structures of the disease variant bound to peptides derived from two PCNA partner proteins reveal that the binding pocket can adjust conformation to accommodate some ligands, indicating that the binding site is dynamic and pliable. (rcsb.org)

A similar murine protein interacts with TNFR-associated factor 1 (TRAF1), TNFR-associated factor 2 (TRAF2), and cylindromatosis. (wikipedia.org)
TRAF interacting protein has been shown to interact with FLII, TRAF1 and TRAF2. (wikipedia.org)

These proteins regulate selective transport, but exactly how they do this is still unclear. (rug.nl)

Smith UM, Consugar M, Tee LJ, McKee BM, Maina EN, Whelan S. The transmembrane protein meckelin (MKS3) is mutated in Meckel-Gruber syndrome and the wpk rat. (medscape.com)

By identifying many sGAG-specific interacting proteins, our data provide a resource for upcoming studies aimed at molecular mechanisms and understanding of sGAG cellular effects. (degruyter.com)
In an attempt to understand the function of APP, in vitro studies have focused on the identification of interacting proteins. (jneurosci.org)
Prior studies of acute phosphate restriction during the endochondral phase of fracture healing showed delayed chondrocyte differentiation was mechanistically linked to decreased bone morphogenetic protein signaling. (researchgate.net)
She was not involved in Weidner's research but has published studies showing that storm clouds can harbor ice-nucleating bacteria. (insidescience.org)
Instead, as in previous studies, FGF13 interacted with Na V 1.7. (jneurosci.org)
In further studies, two RAD1-interacting proteins were identified. (uni-koeln.de)
The points in the figures showing no-observed-adverse-effect levels (NOAELs) or lowest- observed-adverse-effect levels (LOAELs) reflect the actual doses (levels of exposure) used in the studies. (cdc.gov)
However, only a handful of studies have examined the effect of DENV infection in A. aegypti, showing a reduction in lifespan and fecundity over multiple blood meals. (bvsalud.org)
This study provides a functional framework for studies on these previously uncharacterized proteins. (lu.se)
Previous studies by the same researchers have shown that C3 is also present inside cells and plays an important role there. (lu.se)

NUDF protein, the product of the nudF gene, displays 42% sequence identity with the human protein LIS1 required for neuronal migration. (xenbase.org)

PCNA binding partners carry short motifs known as the PCNA-interacting protein-box (PIP-box). (pasteur.fr)
Among the 87 proteins that contained calmodulin-binding motifs, 48 proteins have not previously been shown to interact with calmodulin and 39 proteins were known calmodulin-binding proteins. (lu.se)

Blackman LM, Arikawa M, Yamada S, Suzaki T, Hardham AR (2011) Identification of a mastigoneme protein from Phytophthora nicotianae . (springer.com)

B) Complementation of the nudE deletion and the nudF7 mutant by extra copies of nudE, the nudE NH2-terminal domain, and nudE chimeras carrying coiled-coil regions from human and frog proteins, respectively. (xenbase.org)
However, Y-complex proteins show distinguishable protein dynamics, and the deletion of yaaT or ylbF shows a minor effect on the dynamics of YmcA. (uni-marburg.de)

We showed that the GPI-anchor, which is necessary for CD59 complement inhibitory function, is not necessary for its ability to mediate insulin secretion. (lu.se)
How proteins mediate this curvature generation is a fundamental question that is still poorly understood. (lu.se)

Discovering more about how host cells and pathogens interact could also have important implications for understanding mechanisms of infectious diseases, and how to tackle them. (imperial.ac.uk)

3.0: hematopoietic cell-specific Lyn substrate 1 (HCLS1), Kelch domain-containing 6 (KLHDC6), sarcoglycan delta (SGCD), KIAA1706 protein (KIAA1706), RNA guanylyltransferase and 5′-phosphatase (RNGTT), chromosome 10 open reading frame 57 (C10orf57), chromosome 9 open reading frame 52 (C9orf52), and plasminogen activator, urokinase receptor (PLAUR). (molvis.org)

Calmodulin is an essential Ca2+-binding protein that binds to a variety of targets that carry out critical signaling functions. (lu.se)

The p53 interacting partner protein NUMB (homology of numb protein) preserves this intrinsic cellular asymmetry by preventing ubiquitin-mediated proteolysis of p53 catalyzed by the MDM2 E3 ubiquitin ligase 11 . (nature.com)
A) Homology between A. nidulans NUDE protein and N. crassa RO11 protein. (xenbase.org)

Recombinant human bone morphogenetic protein 2 (rh. (researchgate.net)
Recombinant human bone morphogenetic protein 2 (rhBMP2) is a. (researchgate.net)

The HuProt microarray results showed that the signals of 343 proteins were higher in the recombinant CRYAA group than in the control group. (molvis.org)

Several non-conservative differences in residues that interact with the ligand were identified between the mammalian and parasite ACBPs. (rcsb.org)
Strains were transformed with nudE variants in pAid vector and grown at 43°C. Numbers refer to amino acid residues of NUDE protein expressed by the constructs (see Fig. 3 for detailed amino acid sequences). (xenbase.org)

Thus, this study demonstrates that using proteomic methods on our transgenic model can uncover important in vivo APP-interacting proteins that will provide insights into the biology of APP. (jneurosci.org)
Thus, this study demonstrates that our transgenic model can uncover important in vivo APP-interacting proteins that will contribute to our understanding of APP processing in in vivo settings. (jneurosci.org)

The objective of this research is to determine the biochemical functions of Hessian fly effectors and their interacting proteins in wheat plants. (usda.gov)
Hessian fly larvae are thought to inject secreted salivary gland protein (SSGP) effectors into wheat seedlings to help obtain nutrients and dampen the host resistance response. (usda.gov)
We will use the pEAQ-HT-DEST1 protein transient expression vector system to screen for candidate effectors that can trigger measurable plant responses. (usda.gov)

There is still so much we don't know about DNA, but our results show that how and where G-quadruplex structures form affects their function, making them more important biologically than previously thought. (worldhealth.net)
While that is common in freezing water, it's not something that happens with protein structures. (insidescience.org)

Blocking access to the human proteins would allow our immune systems to kill the virus. (news-medical.net)

Consistently, Txnip-KO mitochondria were functionally and structurally altered, showing reduced oxygen consumption and ultrastructural derangements. (jci.org)
The fact that NUDF and LIS1 interact with the same protein domain strengthens the notion that these two proteins are functionally related. (xenbase.org)

The His-tag may be also used to immobilize proteins to a solid support (such as resin) for identifying interacting targets in wheat. (usda.gov)
To identify interacting targets, Hessian fly-infested and non-infested wheat extracts will be incubated with immobilized family 31 proteins. (usda.gov)

However, little is known about how the protein interactome changes across environmental perturbations. (nature.com)
Current technologies, which assay one PPI at a time, are too low throughput to make it practical to study protein interactome dynamics. (nature.com)
Unknown is how the protein interactome changes as cells differentiate or react to stress, even though these are each fundamental biological processes 10 . (nature.com)
Describing the global protein interactome dynamics, however, requires overcoming two main hurdles. (nature.com)
Observing how the protein interactome changes across perturbations would require re-assaying all ∼ 18 million strains for each perturbation studied. (nature.com)

CRYAA interacted with these proteins to maintain their solubility and decrease the accumulation of denatured target proteins. (molvis.org)

In Saccharomyces cerevisiae , the telomere-associated protein Ndj1 is required for bouquet formation. (biorxiv.org)

Expression Levels of SMAD Specific E3 Ubiquitin Protein Ligase 2 (Smurf2) and its Interacting Partners Show Region-specific Alterations During Brain Aging. (hacettepe.edu.tr)
Cells lacking ORCA show alterations in chromatin architecture, with significantly reduced H3K9 di- and tri-methylation at specific chromatin sites. (nih.gov)

Here, we show that APP can coimmunoprecipitate NEEP21 from nontransgenic mouse brain and from mammalian cells stably coexpressing both proteins. (jneurosci.org)
A special form of four-stranded DNA, recently seen in human cells, has been found to interact with a gene that causes Cockayne Syndrome when faulty. (worldhealth.net)
These special G-quadruplexes arise when distant parts of DNA interact, something that researchers thought was impossible to form within cells. (worldhealth.net)
This involves the study of mesothelioma cells and proteins. (mesothelioma.com)
For example, researchers found a protein on the outside of several types of cancer cells in the 1990s. (mesothelioma.com)
That protein is now called mesothelin, and mesothelioma cells make a lot of it. (mesothelioma.com)
The protein in COVID that enables the virus to make copies of itself, called N, requires the help of human cells to perform its job. (news-medical.net)
Genetic instructions in our cells are transcribed from DNA to messenger RNA, and then translated into proteins that enable functions such as growth and communication with other cells. (news-medical.net)
A small protein on the surface of human cells called CD59 is the body's last line of defence against MAC. (imperial.ac.uk)
This gene provides instructions for making a protein that interacts with other proteins, called receptors, and is likely involved in signaling within cells. (medlineplus.gov)
In paper II, we showed that silencing of CD59 expression in rat β cells significantly suppressed insulin secretion. (lu.se)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
3) The influenza virus buds from host cells in a cholesterol-dependent manner using the matrix protein M2. (lu.se)
Anna Blom and Ben C King have discovered that C3 protein protects insulin-producing cells. (lu.se)
Their research shows that a protein of the immune system protects the insulin-producing cells from inflammation and death. (lu.se)
The protein is secreted from cells and is found in large quantities in the blood. (lu.se)
Now, their latest study in PNAS shows that the protein C3 protects insulin-producing cells from damage and death when it is present inside the cells. (lu.se)
We have chosen a different approach that aims to understand what protects the insulin-producing cells," says Anna Blom, professor of protein chemistry at Lund University, who led the study. (lu.se)
It is already known that a protein called IL-1B can cause inflammation and damage to the insulin-producing cells. (lu.se)

We also show that CXXC1 interacts with IHO1, an essential component of the meiotic double-strand break (DSB) machinery. (springer.com)
These proteins are made up of domains comprising phenylalanine (F) and glycine (G) amino acids in tandem, and these play an essential role in transport. (rug.nl)
[ 14 ] B9d1 and B9d2 are essential components of a B9 protein complex and, when this is disrupted, Meckel syndrome results. (medscape.com)
PolD is an archaeal replicative DNA polymerase, which is widely distributed among Archaea and has been shown to be essential for cell viability. (pasteur.fr)

De Vries: 'However, the experiments showed that transport through the artificial pores occurs but not what happens inside the pore. (rug.nl)
In this case, the team designed and conducted experiments that made COVID proteins' post-translational modifications easy to see. (news-medical.net)
VSPB staff were intrigued when experiments showed that blood samples from some people who had not been vaccinated against Ebola or infected by it were able to interfere with the virus in ways that we would only expect from samples from vaccinees or survivors of Ebola. (cdc.gov)

Although most FGFs are secreted proteins that act by binding to cell-surface receptors, some act intracellularly, usually by interacting with voltage-gated sodium channels. (jneurosci.org)
H1-type histamine receptors were found to be the major mediators of histamine-induced scratching, but FGF13 did not interact directly with or alter the function of these receptors. (jneurosci.org)

In addition, we identified proteins that interact with the KRAB domain of PRDM9 in yeast two-hybrid assay screens, particularly CXXC1, a member of the COMPASS complex. (springer.com)
One pathway, which has been extensively studied in yeast, is mainly guided by chromatin structure and the other, analyzed in detail in mice, is driven by the sequence-specific DNA-binding PR domain-containing protein 9 (PRDM9). (springer.com)
NUDF protein interacts with the Aspergillus NUDE coiled-coil in a yeast two-hybrid system, while human LIS1 interacts with the human homologue of the NUDE/RO11 coiled-coil and also the Xenopus MP43 coiled-coil. (xenbase.org)

Intracellular9

Genes14

FMRP Interacting Protein3

Interactions14

Putative5

Highly conserved3

Interaction11

Membrane17

Adaptor4

Antibodies6

Characterization1

Mass spectrometry3

Actin6

Effector2

Several proteins2

Degradation3

Purification1

Kinase2

Domain-containi1

Mutation5

Bacterial proteins5

Bind3

Preferentially2

Molecules5

Localization3

Complexes1

Mutations5

Client proteins1

Accessory proteins2

Genome2

Affinity3

Coiled-coil domain1

Nuclear6

Lipid2

Pathways1

Regulator1

Mechanism2

Phosphorylation1

Organism1

Peptides1

TRAF22

Regulate1

Transmembrane1

Studies10

Neuronal1

Motifs2

Identification1

Deletion2

Mediate2

Pathogens1

Substrate1

Binds1

Homology2

Human bone2

Signals1

Residues2

Vivo2

Effectors3

Structures2

Blocking1

Functionally2

Targets2

Interactome5

Accumulation1

Saccharomyces1

Alterations2

Cells19

Essential4

Experiments3

Receptors2

Yeast3