Proteins of hct116 colorectal cancer cell. Medical search. Frequent questions

[ 16 , 48 ] In addition, PPAR-β/δ ligands were able to stimulate ANGPTL4 in several lung cancer cell lines, thereby regulating the balance between PPAR-β/δ-mediated cell proliferation and differentiation. (medscape.com)
It was found that vitexicarpin may block the cell cycle in Growth 2 Phase (G2)/Mitosis (M) phase by inhibiting the expression of Cyclin-Dependent Kinase 1 (CDK1), Cellular Myelocytomatosis Oncogene (c-myc) and survivin, thereby inhibiting the proliferation and promoting the apoptosis of human non-small cell lung cancer cell line (H322) [ 3 ]. (ijpsonline.com)
CYY292 dose-dependently inhibited cell proliferation, epithelial-mesenchymal transition, stemness, invasion, and migration in vitro by specifically targeting the FGFR1/AKT/Snail pathways in GBM cells, and this effect was prevented by pharmacological inhibitors and critical gene knockdown. (bvsalud.org)
CYY292 also efficiently reduced GBM cell proliferation and increased survival in orthotopic GBM models. (bvsalud.org)
Finally, overexpression of MALL suppressed HCT116 and SW480 cell proliferation and inhibited HCT116 migration. (oncotarget.com)
Cancer develops when the balance between cell proliferation and cell death is disrupted, and the ensuing aberrant proliferation leads to tumor growth. (aacrjournals.org)
However, a number of recent studies have pointed out that in addition to being an inhibitor of cell proliferation, p21 acts as an inhibitor of apoptosis in a number of systems, and this may counteract its tumor-suppressive functions as a growth inhibitor. (aacrjournals.org)
Active cyclin/Cdk complexes phosphorylate and inactivate members of the retinoblastoma protein (Rb) family that are negative regulators of G 1 and S-phase progression, leading to induction of E2F-regulated gene expression and cell proliferation. (aacrjournals.org)
These proteins play important roles regulating proliferation during normal development and differentiation and after genotoxic stress. (aacrjournals.org)
In glioma ( 28 ) and in vascular smooth muscle cells ( 29 ), p21 facilitates active cyclin-Cdk complex formation and induces cell proliferation. (aacrjournals.org)
2004 . NF-κB activation in human breast cancer specimens and its role in cell proliferation and apoptosis. (nih.gov)
Transfection of full-length E-cadherin cDNA into LS174T cells enhanced intercellular adhesion, induced differentiation, retarded proliferation, inhibited tumorigenicity, and restored responsiveness to the migratory effects induced by the motogenic trefoil factor 2 (human spasmolytic polypeptide). (ox.ac.uk)
The aim of this study was to investigate the inhibitory effect of andrographolide on breast cancer cell line MCF-7 and HCT116 proliferation and its mechanism. (cancercellresearch.org)
Andrographolide on breast cancer MCF-7 and HCT116 cell proliferation has obvious concentration and time dependent inhibition effect, its mechanism may be down-regulating Bal-2 or up-regulating Caspase-3, Caspase-9 protein express. (cancercellresearch.org)
2013). In non-small cell lung cancers (NSCLC) cells, inhibits proliferation and induces cell apoptosis through activating p53 and its own down-stream signaling pathway (Lu et al. (bioinbrief.com)
The proliferation, migration, apoptosis, Tn antigen expression, and O -glycome in Tn + and Tn − CRC cells before and after co-cultured with SP-MSCs were detected using real-time cell Analysis (RTCA), flow cytometry (FCM), and cellular O -glycome reporter/amplification (CORA), respectively. (biomedcentral.com)
Both SP cells derived from hUCMSCs and hPMSCs could inhibit proliferation and migration, promote apoptosis of CRC cells, significantly reduce Tn antigen expression on Tn + CRC cells, generate new core 1-, 2-, and 3-derived O -glycans, increase T-synthase and C3GnT activity, and elevate the levels of Cosmc and T-synthase protein. (biomedcentral.com)
SP-hUCMSCs and SP-hPMSCs could inhibit proliferation and migration and promote apoptosis of Tn + CRC cells via increasing O -glycosyltransferase activity to modify O -glycosylation status, which further adds a new dimension to the treatment of CRC. (biomedcentral.com)
The anti-cancer activities of ginger against colorectal cancer have been well documented with its active component gingerol inhibiting the growth and proliferation of colorectal cancer cells. (superfood.news)

For colon carcinoma cells (HCT-116) Compounds 2a, 2d and 4b gave the strongest activity among all compounds compared with doxorubicin. (dntb.gov.ua)
These findings suggest that the increased expression of pRb in colorectal carcinoma cells may provide a homeostatic mechanism that protects them from growth inhibition and apoptosis, perhaps by counterbalancing potentially toxic effects of excessive E2F activity. (tau.ac.il)
BRCA2 -/- HCT116 human colorectal carcinoma cells were generated to study the role of BRCA2 in DNA repair and to identify additional functions of the BRCA2 gene. (ximbio.com)
Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells. (ox.ac.uk)
The role of interleukin-8 (CXCL8) and CXCR2 in acquired chemoresistance of human colorectal carcinoma cells HCT116. (nih.gov)

We report that higher levels of ADAM10, ADAM17, and ADAM19 were detected in SW480 cells than in HCT116 cells. (frontiersin.org)
Overexpression of ADAM10/17/19 in HCT116 cells enhanced, whereas depletion of ADAM10/17/19 in SW480 cells weakened, migration and invasion. (frontiersin.org)
A , B ) We used TEM (×20,000) to image HCT116 and SW480 cells and quantified the number of autophagic vacuoles (autophagosomes and autolysosomes) in subsets of 10 randomly-selected cells of each type. (aging-us.com)
High MUPCDH methylation was also observed in HCT116 and SW480 CRC cell lines that revealed low gene expression levels compared to COLO205 and HT29 cell lines which lack DNA methylation at the MUPCDH locus. (diagenode.com)
Furthermore, HCT116 and SW480 showed lower levels of RNA polymerase II and histone H3 lysine 4 trimethylation (H3K4me3) as well as higher levels of H3K27 trimethylation at the MUPCDH promoter. (diagenode.com)
MUPCDH expression was however restored in HCT116 and SW480 cells in the presence of 5-Aza-2′-deoxycytidine (DNA methyltransferase inhibitor). (diagenode.com)
Mice treated using a TGFinduces inflammation-linked CRC cell metastatic behaviours We produced a previously set up mobile model using LSMCM lifestyle moderate conditioned by LPS-stimulated monocytes (Supplementary Amount S2A).21, 22 Four (HT29, LoVo, HCT116, and SW480) from the five CRC cell lines (including SW620) actively taken care of immediately LSMCM, morphologically changing into spindle-shaped fibroblast-like cells (Figure 1a). (woofahs.com)
Cell Lifestyle The individual colorectal cancers cell lines RKO, SW1116, HT29, HCT116, LoVo, SW620, SW480 and 293?T were purchased in the Shanghai Institute of Cell Biology, Chinese language Academy of Sciences. (bioinbrief.com)
Table 2 Univariate and multivariate Cox regression analysis H19 for DFS or OS of patients in study cohort (= 83) Manipulation of H19 levels in CRC cells To evaluate the biological functions of H19 we next performed qRT-PCR analysis to examine the expression levels of H19 in a variety of cell lines including HCT116 HT29 SW480 Lovo and the normal colon epithelium cell collection CCD-18Co. (sciencepop.org)

2. ECM Substrates Impact RNAi Localization at Adherens Junctions of Colon Epithelial Cells. (nih.gov)
In normal intestinal epithelial cells, the Wnt signaling is turned off because its downstream mediator β-catenin becomes constitutively phosphorylated and consequently degraded by a so-called "destruction complex" consisting of two protein kinases (GSK3 and CK1), the adenomatous polyposis coli (APC) protein, the E3 ligase TrCP, and the scaffolding protein Axin2 ( Stamos and Weis, 2013 ). (frontiersin.org)
Aberrant activation of the Wnt signaling (by APC loss-of-function mutations) leads to stabilization and subsequent nuclear translocation of β-catenin and boosts malignant transformation of intestinal epithelial cells. (frontiersin.org)
Here, we have explored the molecular mechanism underlying CA-induced Nrf2 activation in colorectal epithelial cells and have examined the chemopreventive potential of CA in a murine colorectal cancer model comparing Nrf2(+/+) with Nrf2(-/-) mice. (nih.gov)
2007 . Activation of both nuclear factor of activated T cells and inhibitor of nuclear factor-κB kinase β-subunit-/nuclear factor-κB is critical for cyclooxygenase-2 induction by benzo[a]pyrene in human bronchial epithelial cells. (nih.gov)
Recently, super-enhancers comprising enhancer clusters co-occupied by lineage-specific factors have been identified in diverse cell types such as adipocytes, hair follicle stem cells, and mammary epithelial cells. (molcells.org)
Up-regulation of the interferon-related genes in BRCA2 knockout epithelial cells. (ximbio.com)
Western blot analysis using an antibody to the gene product confirmed that the protein was overexpressed in nine of the 15 clinical cancer tissues examined, compared to corresponding noncancerous epithelial cells. (lonza.com)

We rationally designed a novel small molecule CYY292, which exhibited a strong affinity for the FGFR1 protein in GBM cell lines in vitro. (bvsalud.org)
This OA significantly inhibited the growth of HepG2 cells in vitro. (bvsalud.org)
Background: We recently reported that ethanol and hexane extracts of the plant product, mastic gum (MG), contain constituents which can induce p53- and p21-independent G 1 -phase arrest followed by apoptosis of human HCT116 colon cancer cells in vitro. (iiarjournals.org)
In this context, several plant products have exhibited chemopreventative activity against colorectal cancer in vitro and in vivo , including buckwheat protein ( 5 ), resveratrol analogs ( 6 , 7 ), linoleic acid conjugates ( 8 ), curcumin ( 9 ), green tea ( 10 ) and grape seed ( 11 ) extracts, and juice or freeze-dried powder from Brussels sprouts ( 12 ), by targeting various molecular and cellular mechanisms. (iiarjournals.org)
Furthermore, it was recently demonstrated that MG constituents can inhibit the expression of the androgen receptor at the transcriptional ( i.e. mRNA) and translational ( i.e. protein) levels in an in vitro model using the androgen-responsive prostate cancer cell line, LNCaP ( 26 ). (iiarjournals.org)
The researchers then examined in vitro (in an artificial environment, such as a test tube) the compound's growth inhibition effect in two human colorectal cancer cell lines. (nih.gov)
The in vitro anticancer activities of each compound were evaluated against colorectal carcinoma Colon cancer (HCT-116) and Epdermoid carcinoma (HEP2) and also Human lung fibroblast normal cell line (WI38) compared with Doxorubicin. (dntb.gov.ua)
In vitro studies were also done to examine the phenotypic effects of an antisense oligodeoxynucleotide (AS-Rb) targeted to Rb mRNA in the HCT116 colon carcinoma cell line that expresses a relatively high level of pRb. (tau.ac.il)
In vitro treatment of HCT116 cells with AS-Rb decreased the level of pRb by about 70% and also decreased the levels of the cyclin D1 protein and cyclin D1-associated kinase activity. (tau.ac.il)
Further cell functional assays in vitro and in vivo were used to explore the roles of AGGF1 in CRC cell migration and invasion, aiming to reveal the promoting roles of AGGF1 in the development of CRC, especially in patients with distant metastasis. (researchsquare.com)
In a study published in Cell and Bioscience , apigenin was reported to suppress various human cancers in vitro and in vivo by multiple biological effects, such as triggering cell apoptosis and autophagy , inducing cell cycle arrest, suppressing cell migration and invasion and stimulating an immune response. (superfood.news)
A study published in Scientific Reports suggested that EGCG can strongly engender apoptosis and inhibit growth in several types of cancers, including brain, breast, colon and kidney cancers, as well as leukemia, as demonstrated by in vivo and in vitro research. (superfood.news)
To test this hypothesis, human dendritic cells (DCs) will be differentiated in vitro from peripheral blood monocytes of healthy donors. (fapesp.br)

A gradual decrease of mRNA levels and protein expression was observed in progressive stages of colorectal carcinogenesis which are consistent with reports of increasing MUPCDH 5′ promoter region DNA methylation. (diagenode.com)
MALL mRNA expression was reduced in the tumor tissues of 70% of the colon cancer patients and 75% of the rectal cancer patients as compared to their normal tissues. (oncotarget.com)
b) qRT-PCR of E-cadherin, Fibronectin, and Vimentin mRNA in HT29 cells treated with LSMCM with/without anti-TGFin LSMCM-induced adjustments in cell behavior. (woofahs.com)
e) Period span of GFI1 and PTGER2 mRNA manifestation in HT29 cells treated with LSMCM by qRT-PCR. (woofahs.com)
We produced eGFP mRNA labeled with fluorophore, enabling our customers to assess its encapsulation efficiency in LNPs and monitor its delivery efficiency in mammalian cells. (tebubio.com)
Tebubio lab has developed a plasmid template for the mRNA production, optimized for efficient transcription and translation in mammalian cells. (tebubio.com)
As a proof of concept, we have produced eGFP mRNA modified with Andy Fluor 647-X-UTP, transfected it in HEK293 cells and detected the mRNA delivery in the cells as well as the eGFP expression by fluorescent microscopy. (tebubio.com)
My experience is beneficial at Tebubio when working on customer's projects with them as well as on R&D, mainly centered around mRNA and cell culture. (tebubio.com)
Whole mRNA was extracted from each cell populations, amplified and analyzed using microarrays. (ncbcs.org)
In the present study, we first identified the expression patterns of AGGF1 at both the mRNA and the protein level in CRC tissues. (researchsquare.com)
We confirmed that combining eIF4A3 with H19 obstructed the recruitment of eIF4A3 to the cell-cycle gene mRNA. (sciencepop.org)
Methods: mRNA expression profiling data in a group of CRC patients (n = 540) was extracted from the Cancer Genome Atlas (TCGA). (bvsalud.org)

We recently showed that treatment of HCT116 cells with a hexane extract of MG (He-MG) can induce arrest at the G 1 -phase of the cell cycle followed by detachment of the cells from the substrate and subsequent apoptosis as assessed by flow cytometry analysis and monitoring of the presence of activated caspases-3, -8, -9 and PARP degradation ( 20 ). (iiarjournals.org)

A disintegrin and metalloproteinase (ADAM) family of proteins play versatile roles in cancer development and progression. (frontiersin.org)
Progression of colorectal cancer from benign colorectal adenoma to malignant carcinoma requires a period of time as a result of the accumulation of variety molecular alterations ( 1 - 4 ). (iiarjournals.org)
Therefore, it is highly desirable to identify and/or develop agents that can inhibit or suppress cancer progression. (iiarjournals.org)
The aim of the present study was to determine whether MALL expression is associated with colon cancer progression and patient survival. (oncotarget.com)
MALL may play a role in colorectal cancer progression as suppression of its expression in tumor tissues negatively impacts colorectal cancer patient survival. (oncotarget.com)
3 complexes regulates progression through the cell cycle (reviewed in Refs. (aacrjournals.org)
CKIs bind and inhibit the activity of cyclin/Cdk complexes and negatively regulate cell cycle progression (reviewed in Refs. (aacrjournals.org)
They play an important role in the occurrence and progression of colorectal cancer, thus providing new targets for targeted therapy of colorectal cancer. (cancercellresearch.org)
Numerous studies have reported the aberrant expression of AGGF1 in cancer tissues and its involvement in malignant progression, such as in gastric cancer, hepatocellular carcinoma, and serous ovarian cancer [ 10-12 ]. (researchsquare.com)
Cosmc (C1GalT1C1) mutation could cause aberrant O -glycosylation and result in expression of Tn antigen on the surface of tumor cells (Tn + cells), which is associated with the metastasis and prognosis of cancer progression. (biomedcentral.com)
In a study published in Food Science & Nutrition , fisetin as a polyphenol with pleiotropic pharmacological properties was found to prevent progression in cell cycle and cell growth and induce apoptosis. (superfood.news)
a positive role in keeping cancer under control, but this also can facilitate cancer progression. (nih.gov)
Defining the epigenetic mechanisms that modulate cancer progression also has great potential for the development of new treatment options not only for treating alcoholics with cancer but also for treating other alcohol-induced diseases. (nih.gov)

The progressive nature of colorectal cancer and poor prognosis associated with the metastatic phase of the disease create an urgent need for the development of more efficacious strategies targeting colorectal carcinogenesis. (nih.gov)
After optimizing colorectal Nrf2 activation and target gene expression by dietary CA-supplementation regimens, we demonstrated that CA suppresses AOM/DSS-induced inflammatory colon carcinogenesis with modulation of molecular markers of colorectal carcinogenesis. (nih.gov)
The incidence of pRb-positive cells was increased during multistage colorectal carcinogenesis. (tau.ac.il)
Gary G. Meadows, Ph.D., consumption and cancer risk and the mechanisms of carcinogenesis. (nih.gov)

Ang-2 and GRP78 are over-expressed in colorectal cancer, representing a molecular marker for malignant degree and infiltration metastasis of colorectal cancer. (cancercellresearch.org)

The expression of gene encoding chromodomain helicase DNA binding protein 6 (CHD6) is frequently altered in tumor tissues of colorectal cancer patients.We performed transcriptomic analysis to identify the differentially expressed genes between control and CHD6 KD HCT116. (nih.gov)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
It is a popular technique for the quantitative characterization of small molecular compounds in biological cells, tissues, organs or organisms. (wjgnet.com)
To detect expression of Angiopoietin-2 (Ang-2) and Glucose Regulated Protein78 (GRP78) in colorectal carcinoma tissues, and their correlation with clinical pathological features, we examine expression of Ang-2 and GRP78 by immunohistochemical staining of paraffin-embedded tumor specimens from 70 colorectal cancer patients who underwent curative surgery at Qingdao Central Hospital. (cancercellresearch.org)
Tissues Microarray Structure Tumor specimens found in tissues microarrays (TMAs) had been extracted from 371 colorectal cancers sufferers who underwent curative resection at Changhai Medical center of the next Military Medical School from January 2001 to Dec 2010. (bioinbrief.com)
However, at the protein level, AGGF1 expression in CRC tissues was significantly higher than in paired normal mucosa, which showed a clear association with TNM stage, AJCC stage, vascular invasion, and differentiation. (researchsquare.com)
Like most stem cells, they isolated from different tissues were consisting of various subpopulations and have certain heterogeneity which created variations in therapeutic efficacy. (biomedcentral.com)
Physique 1 Relative H19 expression in human CRC tissues Overexpression of H19 is usually associated with tumor differentiation TNM stage and poor prognosis of CRC To further understand the significance of H19 overexpression in colorectal malignancy we set out to identify the potential associations between H19 expression and patients' clinicopathological features. (sciencepop.org)

15. Palladin, an actin-associated protein, is required for adherens junction formation and intercellular adhesion in HCT116 colorectal cancer cells. (nih.gov)
Mucin-like protocadherin is implicated in intercellular adhesion and its expression was found decreased in colorectal cancer (CRC). (diagenode.com)

STZ exhibited considerable antiproliferative activity and induced apoptosis and autophagy in HCT116 and RKO cells. (nih.gov)
Differential effects of 5-fluorouracil and oxaliplatin on autophagy in chemoresistant colorectal cancer cells. (nih.gov)
One of the mechanisms associated with the development of chemoresistance is autophagy, a process of self-degradation, that allows the recycling of damaged organelles and long-lived proteins, thus contributing to cell homeostasis. (fapesp.br)
Since low concentrations of chemotherapeutic agents such as paclitaxel and 5-fluorouracil (5-FU) increase the immunogenicity of tumor cells, we hypothesized that the combination of HCQ and 5-FU would lead to the blockage of autophagy, minimizing the degradation of tumor antigen in the cytosol. (fapesp.br)

In this study, we screened a panel of cancer cell lines, finding CCT-β overexpression in the triple-negative breast cancer cell line MDA-MB-231, colorectal cancer cell lines Colo205 and HCT116, and a gastric cancer cell line MKN-45. (tmu.edu.tw)
Frequent activation/overexpression of Aurora A in human primary prostate cancers has been reported. (biomedcentral.com)

Vitexicarpin also inhibits the growth of lung cancer xenografts in nude mice [ 4 ]. (ijpsonline.com)
Materials and Methods: Control and HCT116 tumor bearing SCID mice were injected intraperitoneally with He-MG at different administration schedules and doses ranging from 100 to 220 mg/kg body weight and tumor growth (size) was monitored. (iiarjournals.org)
In this report, we present experimental findings to demonstrate that He-MG is capable of significantly suppressing growth of HCT116 tumors xenografted in immunodeficient SCID mice. (iiarjournals.org)
The study first evaluated in vivo (within the cell) whether the compound―called oplopantriol A―inhibited tumor growth in mice with colon cancer. (nih.gov)
Mice with transplanted human colon cancer cells received oplopantriol A at 15 mg/kg or 30 mg/kg doses, or a control, every other day. (nih.gov)

Abstract Electrochemical bromination and iodination of some pyrazoles were investigated under constant-current (CC) electrolysis in an undivided electrochemical cell. (dntb.gov.ua)
abstract = "We have previously demonstrated the ability of I-Trp to disrupt the protein-protein interaction of β-tubulin with chaperonin-containing TCP-1β (CCT-β). (tmu.edu.tw)

Like apoptosis and other types of programmed cell death, the cell is involved in causing its own death, and gene expression is required. (wikipedia.org)
Cancer cells are highly dependent on dysregulated gene expression to support their uncontrolled growth and high energy needs. (nih.gov)
Methods used were metastatic invasion assay, gene expression analysis by RT-PCR on hypoxic response and oncopathway genes, and large-scale protein profiling to detect proteomic levels of metalloproteinases and surface proteins known to be involved in colorectal cancer invasion. (tebubio.com)
Tissue-specific transcription is critical for normal development, and abnormalities causing undesirable gene expression may lead to diseases such as cancer. (molcells.org)
Although all different types of human cells share the same genome, their gene expression is spatially and temporally distinct. (molcells.org)
Although the role of individual enhancers in cell-type specific gene expression has been studied for a long time, it is only recently that genome-wide analysis has allowed the identification of all enhancers in the mammalian genome. (molcells.org)
Then, we applied it on a gene expression dataset of colorectal cancer clinical patient samples and inferred the perturbed miRNA regulatory networks of colorectal cancer, including several known oncogenic or tumor-suppressive miRNAs, such as miR-17, miR-26 and miR-145. (biomedcentral.com)
After analysing gene-expression profiles of colon cancers on a cDNA microarray containing cDNAs corresponding to 23 040 human genes, we focused on a gene annotated as C10orf3 (chromosome 10 open reading frame 3), whose expression was elevated in colorectal cancers (CRC) as well as in tumors arising in the stomach, lung, pancreas, and breast. (lonza.com)

2016), and a sophisticated CRC cells chemosensitivity to oxaliplatin (Li et al. (bioinbrief.com)

These results indicate that, although inactivating E-cadherin mutations occur relatively infrequently in colorectal cancer cell lines overall (3/43 = 7%), they are more common in cells with an RER+ phenotype (3/10 = 30%) and may contribute to the dysfunction of the E-cadherin-catenin-mediated adhesion/signaling system commonly seen in these tumors. (ox.ac.uk)
This paper explores the potential association of p38 mitogen-activated protein kinase/prostaglandin-endoperoxide synthase 2 related targets and pathways with the development of laryngeal cancer in the context of the mechanisms of action of these pathways in a variety of tumors, with the aim of predicting possible targets for drug treatment of laryngeal cancer. (ijpsonline.com)
Head and neck tumors are the sixth most common malignant tumors in the world, in which Squamous Cell Carcinoma (SCC)[ 1 ] predominantly, accounting for approximately 90 % of cases. (ijpsonline.com)
Although AURKA inhibitors have moved to phase III clinical trials in lymphomas, there has been slower progress in GI cancers and solid tumors. (biomedcentral.com)
Its anti-cancer effects have been studied for brain tumors, breast cancer, colon cancer and lung metastases. (superfood.news)
A study published in BMC Cancer found that curcumin inhibits angiogenesis in some tumors by suppressing angiogenic cytokines. (superfood.news)

Laryngeal cancer is a malignant tumor of the head and neck with clinical features of high recurrence and metastasis and a poor prognosis. (ijpsonline.com)

12. Apical junction complex protein expression in the canine colon: differential expression of claudin-2 in the colonic mucosa in dogs with idiopathic colitis. (nih.gov)
18. Expression and subcellular localization of apical junction proteins in canine duodenal and colonic mucosa. (nih.gov)
This study has compared MUPCDH (CDHR5) expression in three key types of colorectal tissue samples, for normal mucosa, adenoma, and carcinoma. (diagenode.com)
In the present study, we compared the levels of expression of the Rb protein (pRb) in normal human colorectal mucosa, adenomatous polyps, and carcinomas by immunohistochemistry. (tau.ac.il)

Epithelial (E)-cadherin and its associated cytoplasmic proteins (alpha-, beta-, and gamma-catenins) are important mediators of epithelial cell-cell adhesion and intracellular signaling. (ox.ac.uk)
These results also indicate that normal E-cadherin-mediated cell adhesion can restore the ability of colonic tumor cells to respond to trefoil factor 2. (ox.ac.uk)
A study published in Molecules indicated that kaempferol exerts its anti-cancer activity by preventing cell migration and invasion, inhibiting MMP-3 protein, downregulating AKT phosphorylation and increasing the focal adhesion kinase (FAK) activity. (superfood.news)

In the cellular extract prepared from the colorectal cancer cell line HCT116 oxaliplatin showed a prolonged half-life of 3.5 ± 0.3 h and 4.2 ± 0.4 h, assuming zero order or first order kinetics, respectively. (rsc.org)

2012). In a variety of types of cancers, genomic deletion and unusual methylation in the promoter of had been noticed, resulting in the down-regulation of in tumor tissue (Bando et al. (bioinbrief.com)

To observe the short-term effects and adverse reaction of FOLFIRI for gastric cancer patients with metachronous ovarian metastasis. (cancercellresearch.org)

Paraptosis-like phenotype has also been described in human colorectal cancer cells following overactivation of the non-receptor tyrosine kinase c-Src suggesting potential involvement of Src-signalling in paraptosis. (wikipedia.org)
Moreover, based on an RNA-seq analysis, STZ was shown to regulate signal transducer and activator of transcription 3 (STAT3) and the mitogen-activated protein kinase (MAPK) signaling pathways. (nih.gov)
Our results suggested that lactobacilli maybe effectively reduce the numbers of S. typhimurium, IL-8 levels and the anti-apoptotic phosphorylated-p38 mitogen-activated protein kinase and B-cell lymphoma 2 proteins. (iseki-food-ejournal.com)
The cyclin-dependent kinase inhibitor p21 is induced by both p53-dependent and -independent mechanisms following stress, and induction of p21 may cause cell cycle arrest. (aacrjournals.org)
Further examination showed that KUD773 inhibited tubulin polymerization and induced an increase of mitotic phosphoproteins and polo-like kinase 1 (PLK1) phosphorylation, indicating a mitotic arrest of the cell cycle through an anti-tubulin action. (biomedcentral.com)
The regulation of Aurora A RNA, protein and kinase activity is dependent of cell cycle with peaking in the transition of G2 to mitotic phase [ 8 ]. (biomedcentral.com)
p38 mitogenactivated protein kinase/prostaglandin-endoperoxide synthase 2-related targets and signalling pathways have repeatedly been shown to play an important role in the development of tumours, including laryngeal cancer. (ijpsonline.com)
The study of p38 Mitogen-Activated Protein Kinase (p38 MAPK)/Prostaglandin- Endoperoxide Synthase 2 (PTGS2) related targets and signaling pathways can help to elucidate and refine the biological characteristics of laryngeal cancer, and provide guidance for the diagnosis and treatment of laryngeal cancer and the development of laryngeal cancer drugs. (ijpsonline.com)
A study published in Chemical Biology & Drug Design found that cyanidin produces its effects against cancer by inhibiting [RAS (reticular activating system) and MAPK (mitogen-activated protein kinase)] and activating (caspases-3 and P-38) innovative molecular pathways. (superfood.news)

Along with PPAR-α agonists, ANGPTL4 expression stimulated by chemical PPAR-β/δ ligands has been studied in hepatic and intestinal cell lines, and increased expression levels could be associated with decreased triglyceride metabolism. (medscape.com)
6. PLEKHA7, an Apical Adherens Junction Protein, Suppresses Inflammatory Breast Cancer in the Context of High E-Cadherin and p120-Catenin Expression. (nih.gov)
7. Cell-specific diversity in the expression and organization of cytoplasmic plaque proteins of apical junctions. (nih.gov)
Low PHLDA2 expression enhances autophagic flux in CRC cells. (aging-us.com)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
Screening of acetaminophen-induced alterations in epithelial-to-mesenchymal transition-related expression of microRNAs in a model of stem-like triple-negative breast cancer cells: The possible functional impacts. (cancerindex.org)
In the current review, we discuss the role of p21 in regulating cell death and the potential relevance of its expression in cancer. (aacrjournals.org)
The retinoblastoma (Rb) gene is inactivated in a variety of human cancers, but in colorectal carcinomas there is frequently increased expression of this gene. (tau.ac.il)
To select the best production conditions in HEK 293 EBNA cells, we first performed a small volume expression study to define optimal production time and ratio of DNA/transfection reagent. (tebubio.com)
Moreover, we tested addition to the cell culture medium of various supplements (amino acids, inorganic salts, lipids…), aiming at boosting the expression level by varying medium composition. (tebubio.com)
Transcriptional activity of the IFN-stimulated response element (ISRE) was increased in BRCA2 knockout cells, and the expression of BRCA2 greatly decreased IFN-α stimulated ISRE reporter activity. (ximbio.com)
Much evidence exists suggesting a tumor/invasion suppressor role for E-cadherin, and loss of expression, as well as mutations, has been described in a number of epithelial cancers. (ox.ac.uk)
In LS174T such a mutation inevitably contributes to its lack of E-cadherin protein expression and function. (ox.ac.uk)
Through this experiment, the correlation of Ang-2 and GRP78 expression with clinicopathological parameters of colorectal cancer was analyzed. (cancercellresearch.org)
2013). Oddly enough, for tumor cells with p53 deletion, over-expression also inhibits tumorigenesis through concentrating on the microRNAs such as for example microRNA-421 and microRNA-184 (Zhang et al. (bioinbrief.com)
However, the differential expression as well as the biological functions of AGGF1 in colorectal cancer ( CRC ) remain to be established. (researchsquare.com)
Expression of short interfering RNA in cells derived from CRC caused significant decreases in C10orf3 expression and inhibited growth of the transfected cells, which was associated with increased apoptotic cells. (lonza.com)
After treatment, cells will be collected and evaluated by flow cytometry for the expression of molecules involved in their ability to activate/suppress lymphocytes. (fapesp.br)
Expression in the lipase fusion protein was then induced by addition of isopropyl B thiogalactosid to a last concentration of 1 mM and incubation for 1 hour. (ras-signal.com)
The obtained outer membrane preparations inhibitor expert were then subjected to SDS Page to analyze the expression from the lipase fusion protein. (ras-signal.com)
Inducing the pro tein expression of E. coli BL21 pAT LipBc resulted in expression of the lipase fusion protein with a dimension of 82 kDa. (ras-signal.com)
Moreover expression profile data showed that H19 upregulated a series of cell-cycle genes. (sciencepop.org)
Figure3A) 3 which suggests that an increase in the expression levels could be Desacetyl asperulosidic acid significant in colorectal. (sciencepop.org)

Additionally, research found that caspase inhibitors (zVAD.fmk, p53, BAF), x-chromosome-linked inhibitor (xiap), and Bcl-xL( from the Bcl-2 family) did not prevent cell death in 293T cells when induced by IGFIR-IC. (wikipedia.org)
Therefore, paraptosis was concluded to differ from apoptosis (cell death type 1) in being unaffected by inhibitors of apoptosis. (wikipedia.org)
Dong G, Iyamu ID, Vilseck JZ, Chen D, Huang R. Improved Cell-Potent and Selective Peptidomimetic Inhibitors of Protein N-Terminal Methyltransferase 1. (medkoo.com)
The result reveals that compound 2b and have the strongest inhibitory activity of the VEGFR2 and FGFR1 proteins indicating that these substances might conceivably operate as VEGFR2 and FGFR1 inhibitors and hence might take role in anticancer activities with various binding interactions. (dntb.gov.ua)
Our customer needed to investigate whether modulating key culture parameters could improve the predictivity of their colorectal cancer cell-based methodology used to select CDK8 inhibitors and/or identify biomarkers. (tebubio.com)
On each model, a viability assay was performed after 3 days incubation time with inhibitors, followed by large-scale proteome profiling analysis on 2000 targets from each cell lysates. (tebubio.com)
Chemoprevention of colorectal cancer with cyclooxygenase-2 inhibitors: two steps forward, one step back. (nih.gov)
Cyclooxygenase-2 (COX-2), aromatase and breast cancer: a possible role for COX-2 inhibitors in breast cancer chemoprevention. (nih.gov)
This cell line shows phenotypes consistent with previous reports, including loss of Rad51 foci in the presence of double-strand breaks, chromosomal rearrangements and elevated sensitivity to the DNA-damaging agents phleomycin and Parp1 inhibitors. (ximbio.com)
Ongoing clinical trials testing AURKA inhibitors as a single agent or in combination with conventional chemotherapies are expected to provide important clinical information for targeting AURKA in GI cancers. (biomedcentral.com)
After insertion into plasmid pCD003 cleaved with XhoI and KpnI too, plasmid pAT LipBc was obtained encoding a fusion protein comprising Inhibitors,Modulators,Libraries the signal peptide of CtxB at the N terminus followed by the lipase as being a passenger, the linker region along with the B barrel through the AIDA I autotransporter required for outer membrane translocation and complete surface accessi bility. (ras-signal.com)

a) Cytokines in tradition moderate from HT29 cells treated with LSMCM for 6 or 24?h were measured by Cytokine antibody arrays. (woofahs.com)
d) HT29 cells had been incubated with LSMCM for 96?h. (woofahs.com)
To research the factors traveling these adjustments, we analyzed the manifestation of multiple effectors in HT29 cells for 96?h. (woofahs.com)

IL-8 production in colon cancer cells was significantly reduced by these lactobacilli. (iseki-food-ejournal.com)

BRAC2 inherited mutations, predispose carriers to various early onset cancers, including breast and ovarian. (ximbio.com)

Obtaining high yields of pure and active difficult-to-produce recombinant proteins can be a real challenge and may require significant investment. (tebubio.com)

Impact of an anti-cancerous drug was investigated on colon cell lines (HT-29, HCT116 and LS174T) in conventional 2D monolayer and 3D spheroid cultures, in hypoxia or normoxia. (tebubio.com)
In addition to silent changes, polymorphisms, and intronic variants in a number of the cell lines, we detected frameshift single-base deletions in repeat regions of exon 3 (codons 120 and 126) causing premature truncations at codon 216 in four replication-error-positive (RER+) cell lines (LS174T, HCT116, GP2d, and GP5d) derived from 3 patients. (ox.ac.uk)
Tn + cells (LS174T-Tn + and HT-29-Tn + cells) and matching Tn − cells (LS174T-Tn − and HT-29-Tn − cells) were isolated from human colorectal cancer cell (CRC) lines LS174T and HT-29 by immune magnetic beads. (biomedcentral.com)

In HCT116 cells, CA caused a Keap1-C151-dependent increase in Nrf2 protein half-life via blockage of ubiquitination with upregulation of cytoprotective Nrf2 target genes and elevation of cellular glutathione. (nih.gov)
Search miRCancer for let-7c associations with cancer and associated genes. (cancerindex.org)
My work particularly focused on finding new genes responsible for genetic diseases in human and modeling them in cell culture (commercial cell lines, patients' derived cell lines, iPSCs, brain organoids) and animal models (zebrafish & mouse). (tebubio.com)
These outcomes point out that SSc myofibroblasts characterize a singular cell lineage expressing a selected transcriptome that features very excessive ranges of transcripts equivalent to quite a few NBPF genes. (ncbcs.org)
The pathogenesis of laryngeal cancer is not clearly understood, but it is undoubtedly a complex developmental process involving multiple pathways and genes. (ijpsonline.com)
The new score better reflects the systematic importance of genes in cells and it can also be used to analyze miRNA target set enrichments. (biomedcentral.com)

MethodsRetrospective analysis was performed on breast cancer patients who underwent ELEKTA infinity accelerator radiotherapy in sun yat-sen university cancer center from July 13， 2021 to October 15， 2022. (bvsalud.org)

Scratch test was used to detect the migration distance of cells. (ijpsonline.com)
In the present study, we investigated the role of ADAM proteins in colorectal cancer (CRC) cell migration and invasion focusing on the epigenetic mechanism whereby ADAM transcription is regulated. (frontiersin.org)
Clearly, in order to devise a viable interventional approach to treat CRC, it is essential to close the gap in our understanding of the molecular mechanism that governs the malignant migration and invasion of CRC cells. (frontiersin.org)

It is found that Traditional Chinese medicine (TCM) has certain curative effect and value in anticolorectal cancer, so it is of great significance to develop more new TCM for its treatment [ 1 , 2 ]. (ijpsonline.com)

Colorectal cancer is a common malignant tumor in the digestive system, which seriously endangers human health. (ijpsonline.com)
Colorectal cancer (CRC) has become the third most frequently occurring malignant tumor worldwide. (nih.gov)

Notably, the inhibitory activity of GD562 for the SET protein increased over 6-fold compared with the previously reported cell-potent inhibitor DC541. (medkoo.com)

Since joining Tebubio, the subsequent experience I have gained through the many biomarker, bioassay and cell biology projects I have managed, enables me to maximise success rates for our customers, notably by minimising the risk of overseeing important factors. (tebubio.com)
This will improve evaluation of the efficacy of these drugs and establish biomarker based strategies for enrollment into clinical trials, which hold the future direction for personalized cancer therapy. (biomedcentral.com)

Alcohol use and abuse have been limited to, esophageal, laryngeal, of cancer. (nih.gov)

The SwissTargetPrediction analysis indicated that STZ binding to IDO1 was significantly and highly probable, and STZ was found to downregulate IDO1 in CRC cells in a dose-dependent manner. (nih.gov)
The colony forming capacity of BRCA2 knockout cells was significantly reduced in the presence of either IFN-β or IFN-γ. (ximbio.com)

Cumulative evidence suggests that the redox-sensitive transcription factor Nrf2 (nuclear factor-E2-related factor 2), a master regulator of the cellular antioxidant defence, represents a promising molecular target for colorectal cancer chemoprevention. (nih.gov)
The remaining gaps in the knowledge on cancer diseases at the cellular and molecular levels are being filled on a daily basis as novel research methods and novel anticancer drugs emerge. (mdpi.com)
GD562 exhibited improved inhibition of the cellular N-terminal methylation levels of both the regulator of chromosome condensation 1 and the oncoprotein SET with an IC50 value of ~50 µM in human colorectal cancer HCT116 cells. (medkoo.com)
To answer questions related to variations at transcription and protein level, our methodology required long-term imaging at controlled temperature and gas levels (CO 2 and O 2 ), on both 3D cellular models and patient-derived samples. (tebubio.com)
I then continued my professional training in a non-academic environment, gaining experience at Pierre Fabre Laboratories in cell biology, cellular models, genetic therapies. (tebubio.com)
The anticancer agents that act on different cellular targets may result in an arrest of the cell cycle at particular phase. (biomedcentral.com)
The miRNA regulatory networks are usually cell-type specific [ 4 ], which makes it impractical to test and verify all miRNAs in all cellular conditions due to the high experimental cost. (biomedcentral.com)
The gene encodes a putative 464-amino-acid protein containing a domain known as AAA (ATPases associated with a variety of cellular activities). (lonza.com)

As such, paraptosis can be prevented by inhibiting specific protein kinases of these pathways. (wikipedia.org)
This study further elucidates the function of FGFR1 in the GBM and reveals the effect of CYY292, which targets FGFR1, on downstream signaling pathways directly reducing GBM cell growth, invasion, and metastasis and thus impairing the recruitment, activation, and function of immune cells. (bvsalud.org)
ECM-degrading proteases as well as the c-MET, Notch, and TGFsignalling pathways regulate tumourCstroma connections and metastasis.13, 14, 15 Among these, TGFsignalling is vital Isochlorogenic acid A supplier for the metastasis of CRC cells. (woofahs.com)
These studies demonstrated that AURKA not only plays a role in regulating cell cycle and mitosis, but also regulates a number of key oncogenic signaling pathways. (biomedcentral.com)
A study published in Current Pharmaceutical Biotechnology found that crocetin affects the growth of cancer cells by inhibiting nucleic acid synthesis , enhancing the anti-oxidative system, inducing apoptosis and hindering growth signaling pathways. (superfood.news)

To investigate the effect and molecular mechanism of vitexicarpin on the biological behavior of colorectal cancer cells is the objective of the study. (ijpsonline.com)
After obtaining my bachelor's degree in Biotechnology at the University Paris Sud, I joined Tebubio as a Lab Technician in 2008, gaining experience working on many different types of projects in Molecular Biology, Protein purification, Bioassays. (tebubio.com)
With the continuous development of immunology, biochemistry and experimental techniques, it has become a current research trend to investigate the pathogenesis of laryngeal cancer at the molecular level. (ijpsonline.com)

Terms such as "cytoplasmic" and "type 3 cell death" had previously been used to describe these forms of cell death. (wikipedia.org)
Instead, paraptosis displays a somewhat primitive cell death path, comparable to necrosis, including characteristic cytoplasmic vacuole formation and late mitochondrial swelling and clumping. (wikipedia.org)
Similar to apoptosis, staining techniques can be used to identify paraptotic cells by highlighting the translocation of phosphatidylserine from the plasma membrane cytoplasmic (inner) leaflet to the cell surface or outer leaflet. (wikipedia.org)
Almost immediately, major structural rearrangements such as rounded cells, cytoplasmic reorganization, and vacuolation of cells undergoing paraptosis can be seen through light microscopy. (wikipedia.org)

Current protocols for therapy inefficiently targets triple negative breast cancer and barely eradicate cancer stem cells. (cancerindex.org)
I have focused my career on the investigation of proteins because of their importance as pharmaceutical targets. (tebubio.com)

All cell lines had been cultured in Roswell Recreation area Memorial Institute (RPMI) 1640 moderate supplemented with 10% fetal bovine serum (FBS) and antibiotics (100?U/mL penicillin, 100?mg/mL streptomycin), within a PA-824 irreversible inhibition humidified atmosphere of 5% CO2 at 37?C. Cell lines had been authenticated by brief tandem do it again polymerase chain response (STR-PCR). (bioinbrief.com)
Mesenchymal stem cells (MSCs) could participate in immunoregulation, tissue damage repair, and tumor inhibition and be seen as an ideal candidate for tumor therapy due to their inherent capacity to migrate to tumor sites. (biomedcentral.com)

This review is the Dorothy O. Kennedy relates the amount and duration of alcohol intake in humans and in animal models of cancer to tumor growth, angiogenesis, invasion, metastasis, immune response, Distinguished Professor of and host survival in specific types and subtypes of cancer. (nih.gov)

We discovered that STZ suppressed tumor growth in an HCT116 nude mouse xenograft tumor model without causing evident cytotoxicity. (nih.gov)

Recently, we synthesized a new group of compounds, unsymmetrical bisacridines (UAs), which exhibit high cytotoxicity against various human cell lines and antitumor potency against several xenografts. (mostwiedzy.pl)
To investigate whether E-cadherin gene (CDH1) mutations occur in colorectal cancer, we screened 49 human colon carcinoma cell lines from 43 patients by single-strand conformation polymorphism (SSCP) analysis and direct sequencing. (ox.ac.uk)
And the effect of AGGF1 on CRC cell malignance was investigated in our established stable AGGF1 upregulated and knockdown CRC cell lines. (researchsquare.com)
Results show that our method outperformed several compared methods in rediscovering the experimentally perturbed miRNAs in cancer cell lines. (biomedcentral.com)

4. PLEKHA7 is an adherens junction protein with a tissue distribution and subcellular localization distinct from ZO-1 and E-cadherin. (nih.gov)
2008 . Chromium in exhaled breath condensate and pulmonary tissue of non-small cell lung cancer patients. (nih.gov)
In this review, we summarize recent findings concerning enhancer function in tissue-specific gene regulation and cancer development. (molcells.org)
Myofibroblasts are the important thing effector cells accountable for the exaggerated tissue fibrosis in Systemic Sclerosis (SSc). (ncbcs.org)

Whole-cell lysates had been prepared in the indicated period factors and analysed for manifestation and phosphorylation from the indicated proteins. (woofahs.com)
Immature DCs obtained at 5 days of culture will be sensitized with lysates of human colorectal cancer cells, HCT-116, previously exposed to the combination of HCQ and 5-FU. (fapesp.br)

Cancer chemoprevention with natural phytochemical compounds is an emerging strategy to prevent, impede, delay or cure cancer. (superfood.news)

Open up in another window Shape 2 Monocyte-derived TGFdownregulates GFI1 and induces EMT and cell metastatic behavior. (woofahs.com)
Drs Ko and Pederson discovered a small molecule called 3BP which throws a "monkey wrench" into the metabolic machinery of the cancer cell, and induces apoptosis via separation of Hexokinase II from the outer mitochondrial membrane. (greenmedinfo.com)
3BP (3 Bromo Pyruvate) is a small non-toxic molecule that induces apoptosis in cancer cells while sparing normal cells, thus providing the most promising cancer treatment we have seen in many years. (greenmedinfo.com)
It inhibits tubulin polymerization associated with an anti-Aurora A activity, leading to Cdk1 activation and mitotic arrest of the cell cycle that in turn induces Bcl-2 degradation and a subsequent caspase activation in HRPCs. (biomedcentral.com)

This is in comparison to apoptosis, in which the proapoptotic protein Bax induced caspase activation and cell death. (wikipedia.org)
Moreover, the pan-caspase inhibitor Z-VAD-fmk did not prevent cell detachment, but it did prevent apoptosis of the attached cells, indicating that the process of cell detachment, but not apoptosis, is independent of caspase activation in He-MG-treated HCT116 cells ( 20 ). (iiarjournals.org)

Clinical Cancer Research , 5 (7), 1805-1815. (tau.ac.il)
Hormone-refractory prostate cancer (HRPC), which is resistant to hormone therapy, is a major obstacle in clinical treatment. (biomedcentral.com)
The overall biological role and clinical significance of long non-coding RNA H19 in colorectal cancer (CRC) remain largely unknown. (sciencepop.org)

The effect of SP cells derived from MSCs on the biological behaviors and the O -glycosylation status of tumor cells remains unclear. (biomedcentral.com)

Anatomy12

Organisms6

Diseases35

Chemicals and Drugs82

Analytical, Diagnostic and Therapeutic Techniques and Equipment59

Phenomena and Processes61

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science6

Health Care24

Geographicals1

Proliferation19

Carcinoma cells5

SW4809

Epithelial Cells8

Vitro13

MRNA12

Treatment of HCT116 cells1

Progression13

Carcinogenesis4

Metastasis of colorectal cancer1

Tissues8

Intercellular adhesion2

Autophagy4

Overexpression2

Mice5

Abstract2

Gene expression8

20161

Tumors6

Poor prognosis1

Mucosa4

Adhesion3

Oxaliplatin1

Types of cancers1

Gastric cancer1

Kinase9

Expression23

Inhibitors11

HT293

Colon cancer cells1

Ovarian1

Recombinant proteins1

LS174T3

Genes6

Patients who underwent1

Migration3

Curative1

Malignant tumor2

Inhibitory activity1

Biomarker2

Esophageal1

Significantly2

Cellular8

Pathways5

Molecular3

Cytoplasmic4

Targets2

Inhibition2

Invasion1

Nude1

Lines4

Tissue4

Lysates2

Chemoprevention1

Induces4

Caspase activation2

Clinical3

Tumor Cells1