Proteins of bacteriophages lambda. Medical search. Frequent questions

Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome, and may have structures that are either simple or elaborate. (wikipedia.org)
Nanopore sequencing revealed that it has a circular double-stranded DNA genome of 45,617 bp, containing typical phage structural proteins and lambda-like lysogeny regulators. (sdu.dk)
Previous research had shown that the lambda phage introduced its own genetic information into that of its host, inserting it at a specific site in the host genome. (phys.org)
Here, we reported the first Oceanospirillum phage, vB_OliS_GJ44, which was assembled into a 33,786 bp linear dsDNA genome, which includes abundant tail-related and recombinant proteins. (biomedcentral.com)
Kinetic analysis of the genome packaging reaction in bacteriophage lambda. (ucdenver.edu)
Packaging of a unit-length viral genome: the role of nucleotides and the gpD decoration protein in stable nucleocapsid assembly in bacteriophage lambda. (ucdenver.edu)
The shell of a nonenveloped virus with even a very small genome must therefore contain a large number of identical protein subunits-at least 60, if the coat-protein gene is to use up less than 25% of the coding capacity in the enclosed nucleic acid. (basicmedicalkey.com)
We are interested in bacteriophage genome organisation, host range, and adaptations to host defence systems. (brounslab.org)

The solution structure of the Oct-1 POU-specific domain reveals a striking similarity to the bacteriophage lambda repressor DNA-binding domain. (embl.de)
A human dwarfism mutation that affects positive control in the related POU domain protein Pit-1 maps to the same region of the HTH motif as do positive control mutations in lambda repressor. (embl.de)
The repressor found in the phage lambda is a notable example of the level of control possible over gene expression by a very simple system. (wikidoc.org)
The lambda repressor is a dimer also known as the cI protein . (wikidoc.org)
Control of bacteriophage lambda repressor synthesis after phage infection: the role of the N, cII, cIII and cro products. (wikidata.org)
The reporter consisted of a C-terminally truncated CD4, incorporating a flexible cytoplasmic linker (CD4tl), fused to the N-terminal DNA-binding domain of the wild type (wt) bacteriophage lambda repressor (CD4tl-lambda) or L57C mutant repressor (CD4tl-lambdaC). (ucsd.edu)
Octamerization of lambda CI repressor is needed for effective repression of P(RM) and efficient switching from lysogeny. (genesandsignals.org)
Changing the mechanism of transcriptional activation by phage lambda repressor. (genesandsignals.org)
Lac DNA, RNA polymerase and cyclic AMP receptor protein, cyclic AMP, lac repressor and inducer are the essential elements for controlled lac transcription. (wikidata.org)

The recombinant protein in Escherichia coli lysates was labelled P2 (P2 star) to differentiate it from the comigrating adhesin protein called P2. (bmj.com)
Enterobacteria phage λ ( lambda phage ) is a temperate bacteriophage that infects Escherichia coli . (wikidoc.org)
Mutations that alter the ability of the Escherichia coli cyclic AMP receptor protein to activate transcription. (genesandsignals.org)
The role of two surface exposed loops in transcription activation by the Escherichia coli CRP and FNR proteins. (genesandsignals.org)
Interactions between the Escherichia coli cyclic AMP receptor protein and RNA polymerase at class II promoters. (genesandsignals.org)
The excisive recombination reaction of bacteriophage lambda involves a specific and efficient juxtaposition of two distant higher order protein-DNA complexes on the chromosome of Escherichia coli. (elsevierpure.com)
Interaction of the recombination pathways of bacteriophage lambda and its host Escherichia coli K12: effects on exonuclease V activity. (wikidata.org)

mPEG-Maleimide (Thiol PEGylation of Proteins) - In vitro folding of KvAP, a voltage gated K+ channel, Biochemistry. (creativepegworks.com)
mPEG-NHS (PEGylated Polymers for Gene Delivery) - BSA Nanoparticles for siRNA Delivery: Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery, International Journal of Biomaterials, Volume 2012, Article ID 584060, 10 pages, Text . (creativepegworks.com)
We therefore performed experiments to evaluate lambda-ZAP bacteriophage-mediated gene transfer and expression in vitro . (biomedcentral.com)
Viable phage particles were produced by in vitro packaging of recombinant phage DNA using a commercial in vitro lambda DNA packaging system (Promega). (careersfromscience.org)

A temperate bacteriophage infecting Pseudomonas aeruginosa PAO1 was isolated from river water. (sdu.dk)

It was d'Hérelle who conducted much research into bacteriophages and introduced the concept of phage therapy. (wikipedia.org)
We isolate and engineer bacteriophages for phage therapy applications. (brounslab.org)

Specifically the work of Hershey, as contributor to the Hershey-Chase experiment in 1952, provided convincing evidence that DNA, not protein, was the genetic material of life. (wikipedia.org)
Many phage have proved useful in the study of molecular biology and as vectors for the transfer of genetic information between cells lambda bacteriophage can also undergo a lytic cycle or can enter a lysogenic cycle, in which the page DNA is incorporated into that of the host, awaiting a signal that initiates events leading to replication of the virus and lysis of the host cell. (genomicglossaries.com)
Applying it to the bistable genetic switch in bacteriophage lambda, we find that cooperative binding between binding sites on the prophage DNA lead to non-Markovian behavior, as quantified by the conditional activity. (elsevierpure.com)
Shenker, JQ & Lin, MM 2015, ' Cooperativity leads to temporally-correlated fluctuations in the bacteriophage lambda genetic switch ', Frontiers in Plant Science , vol. 6, no. (elsevierpure.com)
RNA polymerase, a protein complex responsible for transcribing genetic information, would normally stop reading this information at the end of the bacterial gene and would ignore any viral genes inserted behind it. (phys.org)
Marine bacteriophages play key roles in the community structure of microorganisms, biogeochemical cycles, and the mediation of genetic diversity through horizontal gene transfer. (biomedcentral.com)
Genetic Map of Phage Lambda 5. (biologydiscussion.com)
Lambda phages have considerable potential as gene delivery vehicles due to their genetic tractability, low cost, safety and physical characteristics in comparison to other nanocarriers and gene porters. (biomedcentral.com)

Bacteriophage Lambda binds to the target E. coli cell, the tail tip binding to a maltose receptor. (wikidoc.org)
Bacteriophage lambda interacts with E. coli proteins. (string-db.org)
Synergistic activation of transcription by bacteriophage lambda cI protein and E. coli cAMP receptor protein. (genesandsignals.org)
Phage lambda is a virus of E. coli K12 which after entering inside host cell normally does not kill it in-spite of being capable of destroying the host. (biologydiscussion.com)
A bacterial artificial chromosome (BAC) is a vector system based on the E. coli F-plasmid, cos sites of the lambda phage and loxP of the P1 phage, used for cloning long (150-350 kbp) DNA sequences. (genomequebecplatforms.com)

Bacteriophages are ubiquitous viruses, found wherever bacteria exist. (wikipedia.org)
Their efforts have been focused on bacteriophages-viruses that rely on bacterial hosts for replication. (phys.org)
Bacteriophage A falls under the family Siphoviridae of the Group I (dsDNA viruses). (biologydiscussion.com)
The dimensions of the lambda phage particles are broadly similar to those of many mammalian viruses and recent structural evidence points to a shared ancestry between tailed bacteriophages and mammalian DNA viruses [ 25 ]. (biomedcentral.com)
Enveloped viruses have, in their lipid bilayer, an impermeable barrier between their genomes and the outside environment, reducing the need for continuity of any protein layer. (basicmedicalkey.com)
Viruses that infect Bacteria are called bacteriophages and they are arguably the most abundant and diverse biological entities on earth. (brounslab.org)

mPEG-MAL (Thiol PEGylation of Proteins) - A Computationally Designed Inhibitor of an Epstein-Barr Viral Bcl-2 Protein Induces Apoptosis in Infected Cells, Cell, Volume 157, Issue 7, 19 June 2014, Pages 1644-1656, Text . (creativepegworks.com)
3D structure of the complex consisting of the bacterial RNA polymerase (light and dark gray) and the viral λN protein (red). (phys.org)
The virus uses a trick that prevents the RNA polymerase from terminating the transcription process: It introduces 'lambda-N' (λN), a tiny protein that attaches itself to the host 's RNA polymerase and forces it to continue transcription of the viral genes . (phys.org)
Until now, and despite intensive efforts, researchers had failed to identify how this tiny protein can achieve such a feat. A Berlin-based team of researchers has now been able to visualize the 3-D structure of the RNA polymerase-λN-complex using high-resolution imaging, enabling them to provide a detailed explanation of this viral exploitation. (phys.org)
The nature of this structure told us that the small viral λN protein seals together the two halves of the RNA polymerase, thus preventing it from falling apart once it reaches the stop signal at the end of the bacterial gene," explains one of the study's first authors, Ferdinand Krupp, who is a doctoral student at Charité's Institute of Medical Physics and Biophysics. (phys.org)
Combined with the eight environmental viral contigs from metagenomics, this study helps fill the gap in our understanding of the isolation, genomic and evolutionary development of Oceanospirillum bacteriophages and provides new insights into the interactions between hosts and bacteriophages for these important marine hydrocarbon-degrading microbial populations. (biomedcentral.com)
Interaction of the adenoviral IVa2 protein with a truncated viral DNA packaging sequence. (ucdenver.edu)
Therapeutic vaccines are aimed at promoting regression of HPV-associated lesions by the induction of cellular immune responses directed against viral proteins expressed in tumor cells[ 25 ]. (biomedcentral.com)
To protect it with a gap-free protein shell, 30 Å thick, would require roughly 25,000 amino acid residues-far more than the viral nucleic acid can encode. (basicmedicalkey.com)

citation needed] Phages were discovered to be antibacterial agents and were used in the former Soviet Republic of Georgia (pioneered there by Giorgi Eliava with help from the co-discoverer of bacteriophages, Félix d'Hérelle) during the 1920s and 1930s for treating bacterial infections. (wikipedia.org)

The lambda phage will remain in the lysogenic state if cI proteins predominate, but will be transformed into the lytic cycle if cro proteins predominate. (wikidoc.org)
However, unlike the bacteriophage, which, during a latent (lysogenic) state, integrates its chromosome into the chromosome of the host bacteriophage, the P1 phage maintains the chromosome in the cytoplasm of bacterial cells in the form of a circular covalently closed molecule resembling a plasmid, the size of which is 100 kbp. (genomequebecplatforms.com)

Influenza A interacts with human proteins. (string-db.org)
HPV-16 interacts with human proteins that regulate the cell cycle. (string-db.org)
Here we identify a Wolbachia protein, designated Oscar, which interacts with Masc via its ankyrin repeats. (bvsalud.org)

Identification of the activating region of catabolite gene activator protein (CAP): Isolation and characterization of mutants of CAP specifically defective in transcription activation. (genesandsignals.org)
Recently, traditional isolation methods, complemented by high-throughput sequencing metagenomics technology, have greatly increased our understanding of the diversity of bacteriophages. (biomedcentral.com)
ethnicities for plasmid and bacteriophage isolation were produced with shaking at 37C in Luria-Bertani broth (34). (careersfromscience.org)

Cloning structural genes for Treponema pallidum immunogens and characterisation of recombinant treponemal surface protein, P2 (P2 star). (bmj.com)
In the following paragraphs, we will write genes in italics and their associated proteins in Roman. (wikidoc.org)
The vectors of this series contain genes of the moderate bacteriophage P1, which ensure replication of the phage chromosome in infected bacterial cells. (genomequebecplatforms.com)

The basic format of the yeast-two hybrid system involves the creation of two hybrid molecules, one in which the " bait ' protein is fused with a transcription factor, and one in which the " prey " protein is fused with a related transcription factor . (genomicglossaries.com)
POU proteins are eukaryotic transcription factors containing a bipartite DNA binding domain referred to as the POU domain. (embl.de)
The acronym POU (pronounced 'pow') is derived from the names of three mammalian transcription factors, the pituitary-specific Pit-1, the octamer-binding proteins Oct-1 and Oct-2, and the neural Unc-86 from Caenorhabditis elegans. (embl.de)
Homeobox proteins as sequence-specific transcription factors. (embl.de)
It regulates the transcription of the cI protein and the Cro protein. (wikidoc.org)
The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast. (academicinfluence.com)
Activation and repression of transcription at two different phage phi29 promoters are mediated by interaction of the same residues of regulatory protein p4 with RNA polymerase. (genesandsignals.org)
Activation of prokaryotic transcription through arbitrary protein-protein contacts. (genesandsignals.org)

co-immunoprecipitation Used to determine protein- protein interactions . (genomicglossaries.com)
The protein-protein and protein-DNA interactions within, and between, these complexes were studied by various biochemical techniques and the patterns of synergism among pairs of mutants with marginally impaired recombination function were analyzed. (elsevierpure.com)

Both Int and IHF bind to attP and form an intasome , a DNA-protein-complex designed for site-specific recombination of the phage and host DNA. (wikidoc.org)
Recombination in bacteriophage lambda. (wikidata.org)

Phages are known to interact with the immune system both indirectly via bacterial expression of phage-encoded proteins and directly by influencing innate immunity and bacterial clearance. (wikipedia.org)
The life cycle of lambda phages is controlled by cI and Cro proteins. (wikidoc.org)
For this purpose, recombinant lambda phages were characterized to compare their performance for gene delivery and expression in four cell lines from different sources. (biomedcentral.com)

After all, these 'factories' are sheep, genetically engineered to produce human proteins in their milk. (scienceforpeople.com)

mPEG-Maleimide (Thiol PEGylation of Proteins) - Structural and Biochemical Consequences of Disease-Causing Mutations in the Ankyrin Repeat Domain of the Human TRPV4 Channel, Biochemistry, 2012, 51 (31), pp 6195-6206, Text . (creativepegworks.com)
Homolog detection using global sequence properties suggests an alternate view of structural encoding in protein sequences. (rochester.edu)
SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
SCOP: Structural Classification of Proteins and ASTRAL. (berkeley.edu)

He found that RNR2 RNA levels increased dramatically, even more than the protein levels, upon exposure of cells to DNA damage and that mutations in RNR2 resulted in hypersensitivity to DNA damage. (the-scientist.com)

Creative BioMart offers numerous options for custom protein production services to accelerate kinase/phosphatase biology research and pharmaceutical development. (kinasebiotech.com)
Production of kinases and phosphatases as well as their substrates is essential to study the mechanism of protein phosphorylation and the development of drugs targeting kinase/phosphatase signaling networks. (kinasebiotech.com)

Native P2 protein was present on T pallidum surfaces as shown by radioimmunoprecipitation assays with extrinsically labelled organisms. (bmj.com)
A cross reactive molecule like P2 was not synthesised by the avirulent spirochaete, T phagedenis biotype Reiter, which indicated that P2 is a protein specific to virulent T pallidum organisms. (bmj.com)
As an essential part of living organisms, proteins play an important role in virtually every process within cells, such as cell signaling, cell cycle, cell adhesion, cell metabolism, and immune responses. (kinasebiotech.com)

One recombinant phage was examined further and contained an insert encoding a prominent treponemal 37,000 dalton protein. (bmj.com)

Schematic representation of hTLK1B-pETDUET-1-Lambda Protein Phosphatase expression vector (A) and coexpression of recombinant 6×His-tagged hTLK1B along with bacteriophage lambda protein phosphatase at 25 °C at different IPTG concentrations. (kinasebiotech.com)

A genomic library consisting of partially digested 10 to 20 kilobase pair fragments of Treponema pallidum deoxyribonucleic acid (DNA) was constructed using bacteriophage lambda EMBL-3 as the vector. (bmj.com)
Bacteriophage-Derived Deoxyribonucleic Acid from Innovative Research developed from purified phage whch results in the characteristic five and eight bands after digestion with EcoR I and Hind III respectively. (innov-research.com)

The POUs domain is structurally similar to the DNA-binding domains of the bacteriophage lambda and 434 repressors and 434 Cro. (embl.de)

Researchers hypothesize that abnormal accumulation of this 4 Kd protein in the brain is due to cleavage of a larger precursor protein, called amyloid precursor protein (APP). (everypatent.com)
In the past, researchers have attempted to characterize the abnormal cleavage event through the use of classical protein purification techniques. (everypatent.com)

A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
is usually multifactorial, involving the coordinate action of main adhesin molecules (P1 and P30) and several high-molecular-weight accessory membrane proteins that act in concert with cytoskeletal elements to facilitate the lateral movement and concentration of the adhesin buy Kaempferol-3-rutinoside molecules in the attachment organelle (4, 9, 15, 16, 29, 32, 38, 39). (careersfromscience.org)

Our scientific team is dedicated to helping our customers choose and optimize the most appropriate approach to generate proteins with high purity and high yields for each project. (kinasebiotech.com)

However, until now there has been no isolate of an Oceanospirillum bacteriophage, and so details of their metagenome has remained unknown. (biomedcentral.com)

the creation of a large number of compounds (usually proteins or peptides) through technologies such as phage display . (genomicglossaries.com)

Alternative approach to protein structure prediction based on sequential similarity of physical properties. (rochester.edu)

At the loxP site, plasmid DNA can be cleaved by the Cre protein of the P1 phage, at the cos site by the corresponding enzyme of the lambda phage. (genomequebecplatforms.com)

The head is made of protein of several types and contains a 46,500 bp long genomic (g) DNA. (biologydiscussion.com)

The production of specific proteins is usually achieved by manipulating gene expression in an organism. (kinasebiotech.com)

He mentioned this to David Stillman , who was at Stanford to interview for a faculty position, and who studied cell cycle regulation of proteins as a postdoc in Kim Nasmyth 's lab at the MRC Laboratory of Molecular Biology in the U.K. Stillman pointed out that ribonucleotide reductase was cell cycle regulated-rather than remaining stable, the RNA and protein levels fluctuate throughout the cell cycle. (the-scientist.com)

The phage λ contains double stranded circular DNA of about 17 µm in length packed in protein head of capsid. (biologydiscussion.com)

Little is known concerning lambda phage-mediated gene transfer and expression in mammalian hosts. (biomedcentral.com)

Upon adsorption on the lamb receptor of the host cell, lambda gDNA is injected through the tail which forms a hollow tube through which the DNA passes to the cell. (biologydiscussion.com)

His research in Chlamydomonas reinhardtii intersects with topics in RNA, Molecular biology, Protein subunit and Photosystem I. His studies deal with areas such as Thylakoid, Biophysics and Photosynthetic reaction centre as well as Photosystem I. (research.com)

For example, Wolbachia infection induces male-specific death in the Asian corn borer Ostrinia furnacalis by targeting the host factor Masculinizer (Masc), an essential protein for masculinization and dosage compensation in lepidopteran insects. (bvsalud.org)

For this purpose, we constructed recombinant λ-phage nanobioparticles containing a mammalian expression cassette encoding enhanced green fluorescent protein (EGFP) and E7 gene of human papillomavirus type 16 (λ-HPV-16 E7) using Lambda ZAP- CMV XR vector. (biomedcentral.com)

At its upper end, the λN protein is in contact with RNA (orange) and various regulatory proteins (yellow, blue and green). (phys.org)
Protein phosphorylation is an extremely important regulatory mechanism in various cellular processes. (kinasebiotech.com)
Studying RNR2' s regulatory elements, he found those that were necessary to induce the production of higher protein levels in response to DNA damage and identified factors that bind these DNA elements to mediate the response of RNR2 to DNA damage. (the-scientist.com)

Sequence-specific dynamic information in proteins. (rochester.edu)
These complexes, which mediate synapsis and strand exchange, consist of two DNA sequences, att L and att R, the bivalent DNA binding protein Int, and the sequence-specific DNA bending proteins, IHF, Xis, and Fis. (elsevierpure.com)

An overview of the structures of protein-DNA complexes. (genesandsignals.org)
Kim, S & Landy, A 1992, ' Lambda Int protein bridges between higher order complexes at two distant chromosomal loci attL and attR ', Science , vol. 256, no. 5054, pp. 198-203. (elsevierpure.com)

Discovered by my colleague Matthijs Jore in 2006 in Wageningen (Netherlands), the protein complex consists of 5 different Cas proteins and one crRNA (in green). (brounslab.org)

Sequence-, structure-, and dynamics-based comparisons of structurally homologous CheY-like proteins. (rochester.edu)

Treponemal proteins ranging in molecular weight from 37,000 daltons to 120,000 daltons were identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting of phage lysate proteins. (bmj.com)
Bacteriophage phi 80-induced low molecular weight RNA. (wikidata.org)

In recent work we developed the first bioinformatic representation of protein dynamics, and are currently using this tool, in combination with earlier studies of the static physical properties of amino acid sequences, to elucidate basic mechanisms of protein folding. (rochester.edu)

mPEG-SCM (Amine PEGylation of Proteins) - PEGylation of lysine residues improves the proteolytic stability of fibronectin while retaining biological activity, Biotechnol. (creativepegworks.com)

We have extended our studies to encompass intrinsically disordered proteins, and are using the sequences of those proteins as an added resource in the study of folding and stability in proteins. (rochester.edu)

From these findings, the inventor has discovered that the mRNA for the secreted MuSK, the mRNA for the MuSK receptor type, or the free MuSK protein in blood can be used as a biomarker for aging, as an indicator showing the function of neuromuscular synapse, or a biomarker that allows early diagnosis for amyotrophy. (skycreatives.in)

The IB1cDNA encodes a 714 amino acid protein with a proline-rich region and a putative basic helix-loop-helix domain (bHLH). (justia.com)
A putative cytadhesin-related protein (PvpA) undergoing variation in its manifestation was identified in the avian pathogen gene was cloned, expressed in and and 49% homology was found having a stretch of 205 amino acids of the cytadherence accessory protein HMW3 of strains, ranging from 48 to 55 kDa and caused by several types of deletions occurring in the PvpA C-terminal end and within the two directly repeated sequences. (careersfromscience.org)
ethnicities for manifestation of proteins under T7 promoter control (40) were produced at 30C with shaking in M9 medium (34) supplemented with an amino acid mixture. (careersfromscience.org)

Elledge had found that Rnr2 protein levels increased when yeast cells were grown in the presence of agents that damaged DNA. (the-scientist.com)
It is disclosed herein that the HIV-1 envelope protein gp120 binds to α4β7 integrin on CD4+ T-cells, natural killer (NK) cells and CD8+ T-cells. (justia.com)

His work deals with themes such as Protein subunit, Biogenesis and Arabidopsis, which intersect with Chloroplast. (research.com)

Anatomy3

Organisms33

Diseases1

Chemicals and Drugs73

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Phenomena and Processes78

Disciplines and Occupations1

Information Science3

Health Care4

Genome8

Repressor9

Escherichia7

Vitro4

Temperate1

Phage therapy2

Genetic8

Coli5

Viruses6

Viral9

Treating bacterial1

Lysogenic2

Interacts3

Isolation3

Genes3

Transcription8

Interactions2

Recombination2

Phages3

Human proteins1

Structural4

Mutations1

Kinase2

Organisms3

Recombinant phage1

Schematic1

Deoxyribonucleic2

Repressors1

Cleavage2

Molecules2

Purity1

Isolate1

Peptides1

Similarity1

Plasmid1

Genomic1

Gene expression1

Stable1

Capsid1

Hosts1

Adsorption1

Reaction1

Infection1

Vector1

Regulatory3

Sequence-specific2

Complexes2

Consists1

Structurally1

Molecular weight2

Representation1

Residues1

Stability1

Receptor1

Amino3

Cells2

Subunit1