Proteins mycobacterium tuberculosis. Medical search. Frequent questions

Computational Prediction and Experimental Assessment of Secreted/Surface Proteins from Mycobacterium tuberculosis H37Rv. (ncsu.edu)
In this study, the genome of Mycobacterium tuberculosis H37Rv was screened using Machine Learning tools that included feature-based predictors, general localizers and transmembrane topology predictors to identify proteins that are potentially secreted to the surface of M. tuberculosis, or to the extracellular milieu through different secretory pathways. (ncsu.edu)
The lysA gene of M. tuberculosis H37Rv has been established as essential for bacterial survival in immunocompromised mice, demonstrating that de novo biosynthesis of lysine is essential for in vivo viability. (rcsb.org)
Elevated levels of CTSB was observed in the lungs of mice and rabbits following infection with Mycobacterium tuberculosis (Mtb) H37Rv as well as in plasma from acute tuberculosis patients. (frontiersin.org)

ABSTRACT The tuberculin skin test (TST) has many limitations for the diagnosis of latent tuberculosis (TB) infection. (who.int)

1998 ). Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. (microbiologyresearch.org)
A decade since the availability of Mycobacterium tuberculosis (Mtb) genome sequence, no promising drug has seen the light of the day. (nih.gov)
the complete genome sequence of Mycobacterium tuberculosis continues to provide an invaluable resource to understand tuberculosis (TB), the leading cause of global infectious disease mortality. (jci.org)

Mycobacterium tuberculosis (Mtb), the aetiological agent of tuberculosis, has evolved to scavenge nutrients from the confined environment of host macrophages with mycobacterial ATP-binding cassette (ABC) transporters playing a key role in nutrient acquisition. (warwick.ac.uk)
Mycobacterium tuberculosis (M. tb), also known as Koch's bacillus, is a species of pathogenic bacteria in the family Mycobacteriaceae and the causative agent of tuberculosis. (wikipedia.org)
Mycobacterium tuberculosis , the agent of tuberculosis, is associated with greater morbidity and longer dormancy infection times in humans than any other type of bacterial illness. (biomedcentral.com)

Impact of HIV co-infection on the evolution and transmission of multidrug-resistant tuberculosis. (cdc.gov)

To determine the prevalence of TB infection, NHANES participants 1 year of age and older who consented to this component were skin tested with a tuberculin-purified protein derivative (PPD) product, PPD S-1, the standard antigen. (cdc.gov)
To help distinguish reactions due to Mycobacterium tuberculosis infection from cross reactions due to nontuberculous mycobacterial infection, participants were also skin tested with nontuberculous mycobacterial antigen PPD-B (Battey strain lot 100616, also called the Boone strain of Mycobacterium intracellulare) . (cdc.gov)

The identification of antigens able to differentiate tuberculosis (TB) disease from TB infection would be valuable. (pasteur.fr)
Given the limited availability of carbohydrates within the phagosomal environmental niche during Mtb intracellular infection, our studies suggest that UspABC enables Mtb to optimize the use of scarce nutrients during intracellular infection, linking essentiality of this protein to a potential role in recycling components of cell-wall peptidoglycan. (warwick.ac.uk)
Mycobacterium tuberculosis, the causative agent of human tuberculosis, is forced into latency by nitric oxide produced by macrophages during infection. (ub.edu)
M. tuberculosis infection is extremely difficult to treat mainly because of its adaptive ability to turn a hostile environment within human macrophages (phagocytes) into a friendly niche for its replication. (hindawi.com)
Latent TB infection (LTBI) is a clinical condition associated with only a positive tuberculin skin test (i.e., evidence of infection with M. tuberculosis ) but without clinical or radiographic signs of active disease. (hindawi.com)
The pro-inflammatory cytokine IL-1β is thought to play a major role in host protection against Mycobacterium tuberculosis (Mtb) infection ( 1 - 8 ). (frontiersin.org)
Two kinds of immunologic methods are used for detecting Mycobacterium tuberculosis infection: tuberculin skin tests (TSTs) and interferon-γ release assay (IGRA) blood tests. (cdc.gov)
Together, these tests are the only means for detecting latent M. tuberculosis infection, and they contribute to diagnosing tuberculosis (TB) disease. (cdc.gov)
All HIV-infected persons at risk for infection with M. tuberculosis must be carefully evaluated and, if indicated, administered therapy to prevent the progression of latent infection to active TB disease and avoid the complications associated with HIV-related TB. (cdc.gov)
TB skin testing was added to the NHANES 1999-2000 examination to provide comprehensive data about the extent of tuberculosis infection in the United States. (cdc.gov)

When in the lungs, M. tuberculosis is phagocytosed by alveolar macrophages, but they are unable to kill and digest the bacterium. (wikipedia.org)
The FAS-I enzyme from the bacterium Mycobacterium tuberculosis prefers substrates with carbon chain length from 12 to 24 carbons. (cathdb.info)
Since this person had been previously exposed to the Mycobacterium tuberculosis bacterium, the recipient produced an immune response at the injection site in the form of some degree of inflammation, as was seen in this case, whereupon, this test returned a positive result. (cdc.gov)
This is the bacterium that causes tuberculosis ( TB ). (medlineplus.gov)

Tubersol is one of two purified-protein derivative (PPD) tuberculin products licensed by the Food and Drug Administration (FDA). (cdc.gov)
This image depicts the left forearm of a person who had received a Mantoux skin test, or PPD test (purified protein derivative), having been inoculated with an extract of the tubercle bacillus, administered intradermally. (cdc.gov)
If identifiable risk factors are present, then a purified protein derivative (PPD) should be placed for the child with culture-negative disease. (medscape.com)

Top down characterization of secreted proteins from Mycobacterium tuberculosis by electron capture dissociation mass spectrometry. (tamu.edu)
For comparison, in this top down mass spectrometry (MS) approach the secreted proteins were precipitated from cell culture filtrate, resuspended, and examined directly by electrospray ionization (ESI) Fourier transform MS. The ESI spectra of three precipitates showed 93, 535, and 369 molecular weight (M(r)) values, for a total of 689 different values. (tamu.edu)
A combination of molecular simulation with hydrogendeuterium-exchange mass spectrometry and electron paramagnetic resonance spectroscopy reveal an asymmetry across the membrane: ATP-induced conformational changes in the cytosolic cavity promote unfolded pre-protein structure, while the exterior cavity favours its formation. (elifesciences.org)

The global distribution and success of M. tuberculosis isolates of the W-Beijing genotype have led to the hypothesis that these strains may have selective advantages over other M. tuberculosis strains. (cdc.gov)
To investigate the potential utility of these approaches, we analysed the genomes of 144 Mycobacterium tuberculosis clinical isolates from The Special Programme for Research and Training in Tropical Diseases (TDR) collection sourced from 20 countries in four continents. (biomedcentral.com)
A genome-wide approach was applied to 127 isolates to identify polymorphisms associated with minimum inhibitory concentrations for first-line anti-tuberculosis drugs. (biomedcentral.com)
The duration of tuberculosis treatment depends on the regimen chosen, the patient's clinical and radiographical responses, smear and culture results, and susceptibility of Mycobacterium tuberculosis isolates from the patient or the suspected source case. (who.int)

Overexpression of Rv0494 in Mycobacterium bovis BCG reduced the basal level expression of kas operon genes, thereby suggesting the repressor nature of this protein in fatty acid synthase II regulation. (microbiologyresearch.org)
In Mycobacterium tuberculosis, the genes hsaD (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase) and nat (arylamine N-acetyltransferase) are essential for survival inside of host macrophages. (ox.ac.uk)
The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)

The enzyme catalyzes an essential step in fatty acid biosynthesis, the reduction of the 2,3-double bond in enoyl-acyl-[acyl-carrier- protein] derivatives of the elongating fatty acid moiety. (cathdb.info)

The most frequently used diagnostic methods for tuberculosis are the tuberculin skin test, acid-fast stain, culture, and polymerase chain reaction. (wikipedia.org)
To evaluate the effectiveness of nested polymerase chain reaction (PCR) for diagnosis of extrapulmonary tuberculosis (ETB), as well as the impact of PCR results on clinical management. (scielo.org.mx)

Current efforts are focused on the mechanism of MmpL3 protein from Mycobacterium tuberculosis, the major target of anti-tuberculosis drug discovery efforts. (ou.edu)

The structure of mycobacterial EgtD suggests that this post-translational modification would require a large-scale change in conformation to make the active-site residue accessible to a protein kinase. (unibas.ch)

The mycobacterial cell envelope has been implicated in the pathogenicity of tuberculosis and therefore has been a prime target for the identification and characterization of surface proteins with potential application in drug and vaccine development. (ncsu.edu)

Combating the spread of drug resistant tuberculosis is a global health priority. (biomedcentral.com)
TB389 trade name] is indicated in combination with other tuberculosis medicines for the treatment of drug- resistant tuberculosis caused by Mycobacterium tuberculosis . (who.int)

Although the costs associated with the blood tests themselves can be greater than the cost of TSTs, the use of the blood tests might be more cost-effective in certain settings because their improved specificity in persons who have had previous Bacille Calmette-Guérin (BCG) immunization or exposure to nontuberculous mycobacteria might allow for better targeting of preventive therapy. (cdc.gov)

Ergothioneine has emerged as a crucial cytoprotectant in the pathogenic lifestyle of Mycobacterium tuberculosis. (unibas.ch)
M. smegmatis is commonly used as a model for studying gene function in M. tuberculosis because it proliferates rapidly and is non-pathogenic. (biomedcentral.com)
The enzyme, present in certain pathogenic species of mycobacteria, catalyses the transfer of mycocerosic acids to the two hydroxyl groups at the common lipid core of phthiocerol, phthiodiolone, and phenolphthiocerol, forming dimycocerosate esters. (genome.jp)

Characteristic patterns of Mycobacterium tuberculosis Beijing genotype strains. (cdc.gov)
In 1995, the largest proportion of the Mycobacterium tuberculosis strains from Beijing, China, shared a high degree of similarity in IS 6110 restriction fragment length polymorphism (RFLP) patterns and identical spoligo patterns ( 6 ). (cdc.gov)

In 2019, M. tuberculosis was found in a genetically related complex group of Mycobacterium species called Mycobacterium tuberculosis complex that has at least 9 members: M. tuberculosis sensu stricto M. africanum M. canetti M. bovis M. caprae M. microti M. pinnipedii M. mungi M. orygis It requires oxygen to grow, and is nonmotile. (wikipedia.org)
2) The direct bactericidal activity of HAMLET, a human milk protein-lipid complex, against various bacterial species as well as its adjuvant activity in sensitizing bacteria to a broad range of common antibiotics to provide novel therapeutic strategies against respiratory and other infections with antibiotic-resistant bacteria. (lu.se)

X-ray crystal structures have shown that trHbN hosts a two-branched protein matrix tunnel system, proposed to control diatomic ligand migration to the heme, as the rate-limiting step in NO conversion to nitrate. (ub.edu)
2008. Structural determinants of ligand migration in Mycobacterium tuberculosis truncated hemoglobin O . Proteins. (ub.edu)

We used lymphoproliferation, ELISpot IFN-gamma, cytokine production assays and detection of specific human antibodies against recombinant M. tuberculosis proteins. (pasteur.fr)
Recombinant expression of proteins of interest in Escherichia coli is an important tool in the determination of protein structure. (iucr.org)
There is extensive literature on the variables leading to insoluble recombinant expression of proteins. (iucr.org)

However, in the vast majority of prokaryotes, including M. tuberculosis , CoaB and CoaC are encoded by a single gene to produce a fused bifunctional enzyme (CoaBC). (nature.com)
The Mycobacterium tuberculosis lysA gene encodes the enzyme meso-diaminopimelate decarboxylase (DAPDC), a pyridoxal-5'-phosphate (PLP)-dependent enzyme. (rcsb.org)
In the push for new interventions, we have recently taken the first steps towards a complete reconstruction of the Mycobacterium tuberculosis (MTB) gene regulatory network. (aiche.org)

Coenzyme A (CoA) is a fundamental co-factor for all life, involved in numerous metabolic pathways and cellular processes, and its biosynthetic pathway has raised substantial interest as a drug target against multiple pathogens including Mycobacterium tuberculosis . (nature.com)
The process is highly regulated by multiple signaling pathways and orchestrated by more than 30 autophagy-related (Atgs) proteins organized in several functional units ( 2 ). (frontiersin.org)

An impediment to the successful production of large quantities of natively folded proteins in Escherichia coli is the tendency of many proteins to become insoluble when overexpressed. (iucr.org)

Temperature stability of proteins essential for the intracellular survival of Mycobacterium tuberculosis. (ox.ac.uk)
Macroautophagy, hereafter referred to as autophagy, is a lysosomal degradative process that participates in cellular homeostasis by enabling the removal of defective organelles, protein aggregates, or intracellular microorganisms ( 1 ). (frontiersin.org)

Cellular and humoral immune responses to Erp (Exported repetitive protein)-a recently identified M. tuberculosis protein-have not yet been investigated in humans and may contribute to this aim. (pasteur.fr)
Humans are the only known reservoirs of M. tuberculosis. (wikipedia.org)
The chronic state and dormancy of tuberculosis implies that M. tuberculosis has developed sophisticated strategies to modify and evade the innate and adaptive immune surveillance mechanisms of humans [ 2 ]. (biomedcentral.com)
The bacterial pathogen Mycobacterium tuberculosis (MTB) infects 30% of all humans and kills someone every 20 - 30 seconds. (aiche.org)

These guidelines update previous CDC recommendations for the diagnosis, treatment, and prevention of tuberculosis (TB) among adults and children coinfected with human immunodeficiency virus (HIV) in the United States. (cdc.gov)
Early diagnosis and effective treatment of TB among HIV-infected patients are critical for curing TB, minimizing the negative effects of TB on the course of HIV, and interrupting the transmission of Mycobacterium tuberculosis to other persons in the community. (cdc.gov)

Hit expansion led to the discovery of potent and selective inhibitors of M. tuberculosis CoaB, which we show to bind to a cryptic allosteric site within CoaB. (nature.com)
We also have collaborative interests in determining structures of protein complexes formed with anti-cancer and anti-thrombosis inhibitors. (nottingham.ac.uk)

Capreomycin is an old antibiotic for treatment of pulmonary tuberculosis [ 7 ] with recently increasing interest. (hindawi.com)

Compared to other commonly studied bacteria, M. tuberculosis has a remarkably slow growth rate, doubling roughly once per day. (wikipedia.org)
The slices of the Mycobacterium tuberculosis analyzed under a scanning electron microscope by a Japan-based research group has revealed that bacteria is about 2.71 ± 1.05μm in length with an average diameter of the cell approximately 0.345 ± 0.029 μm. (wikipedia.org)
In routine diagnostic practice susceptibility to anti-tuberculosis drugs is assessed phenotypically by determining the proportion of bacteria that will grow at critical concentrations of the drug [ 5 ]. (biomedcentral.com)
In bacteria, this comprises SecY, SecE and usually SecG, with the protein-conducting pore running through the centre of SecY. (elifesciences.org)
In this review, we intend to summarize the current knowledge on the many functions of autophagy proteins in cell defenses with a focus on bacteria-macrophage interaction. (frontiersin.org)
Furthermore, Atgs proteins have non-autophagic functions essential for innate immunity against bacteria (Figure 1 ). (frontiersin.org)
3. Efflux and export transporters of Gram-positive bacteria and mycobacteria. (ou.edu)
A few viruses that infect bacteria have been carefully studied as laboratory model systems, but bacterial virus are very diverse and as a group they have many proteins with unknown functions. (wikiversity.org)

The Mycobacterium tuberculosis complex CFP-10/ESAT-6 family proteins play essential but poorly defined roles in tuberculosis pathogenesis. (kent.ac.uk)
This analysis identified the molecular basis for the specificity and stability of complex formation between CFP-10/ ESAT-6 family proteins, and indicates that the formation of functional complexes with key roles in pathogenesis will be limited to genome partners, or very closely related family members, such as Rv0287/Rv0288 and Rv3019c/Rv3020c. (kent.ac.uk)
Secreted proteins of Mycobacterium tuberculosis are implicated in its disease pathogenesis and so are considered as potential diagnostic and vaccine candidates. (tamu.edu)

Tuberculosis (TB) is the most prevalent and deadly infectious disease worldwide and remains a global epidemic. (nature.com)
Tuberculosis (TB) and AIDS cause more deaths in adults worldwide than any other infectious disease. (cdc.gov)
Tuberculosis is a leading infectious disease causing millions of deaths each year. (hindawi.com)
Tuberculosis (TB) is a deadly infectious disease caused by Mycobacterium tuberculosis that typically affects the lungs (pulmonary TB) but may also occur in other organs (extrapulmonary TB), such as the central nervous system, lymphatic system, circulatory system, genitourinary system, bones, joints, and the skin. (hindawi.com)

Of nine molecular ions characterized by MS/MS, only one protein match was confirmed, and its isotopic molecular ions were overlapped by those of another protein. (tamu.edu)
Protein structure and interaction modelling are used to understand the functional effects of putative mutations and provide insight into the molecular mechanisms leading to resistance. (biomedcentral.com)
The findings show how a crucial enzyme in our blood, known as ADAMTS13, works like a pair of molecular scissors to carefully cut back the clotting effects of a key protein, von Willebrand factor (VWF). (nottingham.ac.uk)

This is the first report, to the best of our knowledge, of a GntR/FadR family protein acting as a fatty acid-responsive transcriptional regulator in M. tuberculosis , suggesting a possible role for this protein in mycolic acid biosynthesis. (microbiologyresearch.org)
First discovered in 1882 by Robert Koch, M. tuberculosis has an unusual, waxy coating on its cell surface primarily due to the presence of mycolic acid. (wikipedia.org)
However, the mycolic acid in the cell wall of M. tuberculosis does not absorb the stain. (wikipedia.org)

The M. tuberculosis phylogeny c onsists of four major lineages (L1 - Indo-Oceanic, L2 - East-Asian, L3 - East-African-Indian, L4 - Euro-American), which may vary in their propensity to transmit and cause disease [ 2 ]. (biomedcentral.com)
Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease. (ku.dk)
To aid in the interpretation of skin test results and provide for risk factor analysis, participants were asked questions about TB skin testing, disease, exposure to and treatment for Mycobacterium tuberculosis . (cdc.gov)

Mycobacteria growth indicator tubes can contain a gel that emits fluorescent light if mycobacteria are grown. (wikipedia.org)

Additionally, the viability of M. smegmatis in the presence of over-expressed Rv1096 protein was 10 9 -fold higher than that of wild-type M. smegmatis after lysozyme treatment. (biomedcentral.com)

This work shows that the CFP-10/ESAT-6 related proteins, Rv0287 and Rv0288, form a tight 1:1 complex, which is predominantly helical in structure and is predicted to closely resemble the complex formed by CFP-10 and ESAT-6. (kent.ac.uk)
Analysis of the structure of the CFP-10.ESAT-6 complex and optimized amino acid sequence alignments of M. tuberculosis CFP-10/ESAT-6 family proteins revealed that residues involved in the intramolecular contacts between helices are conserved across the CFP-10/ ESAT-6 family, but not those involved in primarily intermolecular contacts. (kent.ac.uk)
Decrease in the number of clinical specimens received and number of patients for whom a specimen was submitted from 2015 to 2017, but an increase of 3.9% for the number of patients positive for Mycobacterium tuberculosis complex (MTBC) by culture. (cdc.gov)
M. tuberculosis has a distinctive cell wall structure, which is called the "core" mycolyl arabinogalactan-peptidoglycan (mAGP) complex. (biomedcentral.com)
Drugs targeted against DAPDC could be efficient anti-tuberculosis drugs, and the three-dimensional structure of DAPDC from M. tuberculosis complexed with reaction product lysine and the ternary complex with PLP and lysine in the active site has been determined. (rcsb.org)
This latter step is mediated by a second Beclin-1 complex, lysosomal-associated membrane protein 1 (LAMP1), and a fusion machinery including SNARE syntaxin-17. (frontiersin.org)

Here we report the first structure of a full-length CoaBC, from the model organism Mycobacterium smegmatis , describe how it is organised as a dodecamer and regulated by CoA thioesters. (nature.com)
patients become sensitized to certain antigenic components of the M. tuberculosis organism. (medscape.com)

At the 25-year anniversary of this accomplishment, we describe how insights gleaned from the M. tuberculosis genome have led to vital tools for TB research, epidemiology, and clinical practice. (jci.org)
The M. tuberculosis genome has ushered in a quarter century of substantial clinical and public health advancements. (jci.org)
For most anti-tuberculosis drugs, a single concentration is used, but for some drugs two concentrations are used to indicate high and low levels of resistance, where increasing the patient dose may be of clinical benefit. (biomedcentral.com)

Our findings suggest that deacetylation of cell wall PG may be involved in evasion of host immune defenses by M. tuberculosis . (biomedcentral.com)

Additionally, light microscopy and scanning electron microscopy showed that in the presence of over-expressed Rv1096 protein, M. smegmatis kept its regular shape, with an undamaged cell wall and smooth surface. (biomedcentral.com)
We also investigated whether the Rv1096 protein in M. smegmatis was lysozyme resistant. (biomedcentral.com)

A cell-penetrant peptide blocking C9ORF72-repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins. (le.ac.uk)

Whole genome association studies are being applied to identify genetic determinants of resistance to anti-tuberculosis drugs. (biomedcentral.com)

In addition, the effect of identified candidate mutations on protein stability and interactions was assessed quantitatively with well-established computational methods. (biomedcentral.com)

The M. tuberculosis genome was sequenced in 1998. (wikipedia.org)

These data indicate that this eukaryotic cell-free expression system has a high success rate and that the addition of specific reagents can increase the yield of soluble protein. (iucr.org)

The first and second domains of TBNAT were demonstrated to be very important to the heat stability of the protein, as the transfer of these domains caused a dramatic reduction in the heat stability. (ox.ac.uk)
A subset of the mutations identified in rpoB and katG were predicted to affect protein stability. (biomedcentral.com)

The structural biology laboratory utilises a variety of techniques including Protein Crystallography, Cryo-electron Microscopy and associated techniques such as Isothermal Titration Calorimetry, which are then combined with functional studies or serve as a basis for structure-based drug design. (nottingham.ac.uk)

Approximately one third of the population worldwide are infected with M. tuberculosis , which causes nearly two million deaths each year [ 1 ]. (biomedcentral.com)

In the map, if you click on the arrow representing the first predicted protein (L5p01) you will go to a page that shows the amino acid sequence for that protein. (wikiversity.org)

Anatomy15

Organisms28

Diseases42

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment44

Phenomena and Processes44

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena3

Information Science5

Health Care10

Geographicals7

H37Rv4

Abstract1

Genome sequence3

Agent of tuberculosis3

Multidrug-resistant tu1

Antigen2

Infection10

Bacterium4

Purified-protein de3

Mass spectrometry3

Isolates4

Genes3

Fatty acid biosynt1

Polymerase chain re2

Anti-tuberculosis drug1

Post-Translational1

Characterization1

Drug resistant tu2

Nontuberculous mycobacteria1

Pathogenic3

Strains2

Species2

Ligand migration2

Recombinant3

Gene3

Pathways2

Escherichia1

Intracellular2

Humans4

Diagnosis2

Inhibitors2

Pulmonary tuberculosis1

Bacteria8

Pathogenesis3

Infectious disease4

Molecular3

Mycolic acid3

Disease3

Growth Indicator1

Viability1

Complex6

Organism2

Clinical3

Defenses1

Smegmatis2

Peptide1

Determinants1

Interactions1

19981

Reagents1

Stability2

Cryo-Electron M1

Million deaths1

Amino1