Proteins liquid liquid separation liquid phases. Medical search. Frequent questions

High-performance liquid chromatography is an appropriate technology for this purpose, and the stationary phase is the kernel to the separation efficiency . (bvsalud.org)
This column was placed automatically in front of a C8 analytical high-performance liquid chromatography column for chromatographic separation of the analytes. (cdc.gov)
In this article, we discuss the role of the gradient in protein separations by reversed-phase and ionexchange high performance liquid chromatography (HPLC). (chromatographyonline.com)
The ether bond internal to N- acetylmuramic acid was cleaved with 3% ammonia, and the resulting lactoyl peptides were separated by reversed-phase, high-performance liquid chromatography for sequencing by tandem mass spectrometry (Qstar Pulsar I). (cdc.gov)
In this study, a normal-phase and reverse-phase two dimensional high performance liquid chromatography (HPLC)-mass spectrometric system was applied to separate oxidized phospholipids from their non-oxidized counterparts, allowing unambiguous detection in a total lipid extract. (cdc.gov)

Liquid-liquid phase separation (LLPS) is well defined in the Biomolecular condensate page. (wikipedia.org)
LLPS databases cover different aspects of LLPS phenomena, ranging from cellular location of the Membraneless Organelles (MLOs) to the role of a particular protein/region forming the condensate state. (wikipedia.org)
Intracellular membraneless organelles (MOs) form dynamically and thermodynamically reversible via liquid-liquid phase separation (LLPS). (spp2191.com)
Proceeds from this financing are anticipated to be used to enhance the Protein Liquid-Liquid Phase Separation (LLPS) technology platform and advance multiple pipelines through discovery and clinical development. (voiceofasean.com)
ETERN has developed a leading-edge Protein LLPS technology platform. (voiceofasean.com)
The biological phenomenon of protein LLPS has attracted strong attention worldwide. (voiceofasean.com)
Dr. Zhu and his team have studied this field extensively and published their key research in the top international journals - Cell and Cell Research, setting forth a new strategy for drug development to regulate protein functions by targeting its LLPS with small molecules and providing a new direction for the development of such drugs to target "undruggable" proteins. (voiceofasean.com)
In the article "Drug Startups Coalesce around Condensates" published by Nature Biotechnology in February 2021 , ETERN was introduced as one of the world's early biotech companies focusing on the research and development of protein LLPS drugs. (voiceofasean.com)
Based on years of basic research, ETERN has built an innovative LLPS technology platform to effectively target the "undruggable" proteins and solve major global unmet medical needs. (voiceofasean.com)
With the world-leading Protein Liquid-Liquid Phase Separation (LLPS) technology platform, ETERN has built multiple pipelines and advanced one to the clinical stage to provide new solutions to cancer patients. (voiceofasean.com)
Here, we found that the C-terminus of IRS-1 undergoes liquid-liquid phase separation (LLPS). (nature.com)
Importantly, metabolic disease-derived G972R mutation results in a reduced ability of LLPS, potentially implicating the involvement of aberrant IRS-1 phase separation in various metabolic disorders. (nature.com)
Pex13 undergoes liquid-liquid phase separation (LLPS) with Pex5-cargo. (tcdb.org)
For protein systems, we focus on block proteins that undergo liquid-liquid phase separation (LLPS) as a preassembly step for structural protein materials. (aalto.fi)
Recent evidence has shown that the processes of liquid-liquid phase separation (LLPS) or liquid-liquid phase transitions (LLPTs) are a crucial and prevalent phenomenon that underlies the biogenesis of numerous membrane-less organelles (MLOs) and biomolecular condensates within the cells. (usf.edu)
Additionally, the finding of mis-regulated proteins, which have long been linked to aggregates in neuropathology, are also known to induce LLPS/LLPTs, prompting a lot of interest in understanding the connection between aberrant phase separation and disorder conditions. (usf.edu)
Recently, it was revealed that the ordered localization of proteins and nucleic acids in the membraneless intracellular microenvironment is modulated by liquid-liquid phase separation (LLPS) ( Shin and Brangwynne, 2017 ). (aspetjournals.org)

Protein aggregation is the main hallmark of neurodegenerative diseases. (nih.gov)
They contain pathogenic forms of proteins that are associated with neurodegenerative diseases su as the enzyme superoxide dismutase (SOD1) involved in familiar amyotrophic lateral sclerosis. (spp2191.com)
In mammals, protein misfolding and aggregation leads to loss of function of the original protein while in many cases being associated with neurodegenerative diseases, eventually leading to death of the organism. (cuny.edu)

Hammer and Lee are now using this platform generate skins that control the liquid properties of condensates and to assemble collectives of coacervates into granular materials. (upenn.edu)
Mitochondrial injury drives redox state-sensitive cysteine-mediated phase separation of ApoE2, forming biomolecular condensates that could nucleate drusen. (jci.org)
We also found that EphA2 can form liquid-liquid phase separation condensates on cell membrane, which can be disrupted by ALW-II-41-27, an inhibitor of EphA2. (aging-us.com)
Currently, I work on topics such as protein folding in cellular environments, protein aggregation, and phase separation of biomolecular condensates. (lu.se)

We developed an innovative methodology to directly measure local electrostatic potentials for every residue of proteins and nucleic acids. (utmb.edu)
Applications to intrinsically disordered proteins and conformationally flexible nucleic acids are particularly useful because structure-based analysis of electrostatics is not straightforward for such molecules. (utmb.edu)
Due to their mobility broadening their probability distributions, the vast majority of counterions around proteins, nucleic acids, and their complexes are unresolved even in high-resolution crystal structures. (utmb.edu)
We use NMR spectroscopy for such characterizations of the positively or negatively charged groups of proteins and nucleic acids. (utmb.edu)
In eukaryotic cells, various proteins and nucleic acids are orderly arranged in the nucleus and cytoplasm ( Handwerger and Gall, 2006 ). (aspetjournals.org)
Applications are invited for two postdoctoral positions to develop computational approaches to study (1) the phenomena of liquid-liquid phase separation of heterogenous mixtures of nucleic acids and proteins occurring inside the Cell nucleus and (2) the organization of chromatin with subnucleosome resolution inside megabase scale domains. (lu.se)

2021) Sequestration of Proteins in Stress Granules Relies on the In-Cell but Not the In Vitro Folding Stability. (spp2191.com)
SHANGHAI , Sept. 1, 2021 /PRNewswire/ - ETERN Therapeutics, a clinical-stage biotech company focusing on developing novel technologies to target "undruggable" proteins, today announced the completion of a Series B financing round. (voiceofasean.com)

Many proteins found in pathological inclusions are known to undergo liquid-liquid phase separation, a reversible process of molecular self-assembly. (nih.gov)
Formation of Aqueous Biphasic Systems with an Ionic Liquid Induced by Metallic Salts: Nanoscopic Views from Molecular Dynamics Simulations. (acs.org)
The molecular processes that drive MO formation are largely unknown, but recent deletion experiments showed that their formation and function is often associated with intrinsically disordered protein domains. (spp2191.com)
Here, we analysed two deep mutational scanning experiments to investigate the role of protein aggregation in molecular evolution. (frontiersin.org)
Here, we set to clarify this complex matter and question of whether aggregation is an important constraint in protein evolution that can be the discriminant between beneficial and detrimental situations by considering two cases: we studied molecular evolution of proteins in an endogenous vs. exogenous host. (frontiersin.org)
1 Sequence dependent phase separation of protein-polynucleotide mixtures elucidated using molecular simulations. (upenn.edu)
The SARS-CoV-2 nucleocapsid (N) protein is an abundant RNA binding protein critical for viral genome packaging, yet the molecular details that underlie this process are poorly understood. (biorxiv.org)
Here we combine single-molecule spectroscopy with all-atom simulations to uncover the molecular details that contribute to N protein function. (biorxiv.org)
We show examples of purely entropic fluid-fluid transitions that precede crystallization, just as liquid-liquid phase separation precedes crystallization in tetrahedrally bonded molecular liquids and in protein solutions. (ucsb.edu)

2023). Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains. (upenn.edu)
2023). Introduction: Phase Separation. (upenn.edu)
2023). Engineering Protein Coacervates into a Robust Adhesive for Real-Time Skin Healing. (news-medical.net)
Expected announcement of the position is spring 2023, and the project will focus on liquid-liquid phase separation mediated by phosphorylated intrinsically disordered proteins utilizing computer simulations in combination with SAXS. (lu.se)

Emerging evidence supports the hypothesis that aberrant phase separation behavior may serve as a trigger of protein aggregation in neurodegeneration, and efforts to understand and control the underlying mechanisms are underway. (nih.gov)
2 Identifying sequence perturbations to an intrinsically disordered protein that determine its phase-separation behavior. (upenn.edu)
As to the role of the cytoskeleton, what does this tell us about the membrane itself, or the behavior of membrane proteins as individual molecules in their interplay with the membrane? (biophysics.org)

Loss of solubility and aggregation can lead to the partial or complete abrogation of protein function. (frontiersin.org)
Thus, understanding the relationship between protein evolution and aggregation is an important goal. (frontiersin.org)
By contrast, in the case of TEM-1 beta-lactamase mutations, aggregation is linked to a decreased cell fitness due to inactivation of protein function. (frontiersin.org)
Given the double valence of protein aggregation that can result either in the formation of toxic assemblies or physiologically required organelles, it has long been debated what could determine whether aggregation is beneficial or detrimental from an evolutionary perspective. (frontiersin.org)
Aberrant Liquid-liquid Phase Separation and Amyloid Aggregation of Pro" by Azeem Ahmad, Vladimir N. Uversky et al. (usf.edu)

Hochmair J, Exner C, Betzel C, Mandelkow E , Wegmann S . Light Microscopy and Dynamic Light Scattering to Study Liquid-Liquid Phase Separation of Tau Proteins In Vitro. (neurotree.org)
Drugs that decrease RPE cholesterol or ceramide prevent mitochondrial injury and phase separation in vitro and in vivo. (jci.org)
CLIP-170 and EB3, when acting together, regulate the speed of microtubule growth at specific locations within the cell.The study used advanced microscopy techniques to observe the role of these two proteins both in vitro and in cellulo, providing insights into their functions.In conclusion, this discovery represents a significant advancement in understanding the regulation of microtubule dynamics within cells. (expertmarketresearch.com)

hnRNPA1 is an RNA-binding protein involved in stress granule formation. (neurosciencenews.com)

These membraneless structures behave as liquid droplets in the cytoplasm or nucleoplasm. (nature.com)
A subunit of the enzymatic complex P-TEFb can induce compartmentalization of proteins into liquid-like droplets in cells. (natureasia.com)
In addition, they provided insight into the critical role of the chromatin network in stabilizing multiple liquid droplets. (nyu.edu)
In this study, researchers showed that under certain conditions related to temperature, salt and protein concentrations, hnRNPA1's disordered tail prompts the protein to condense into liquid droplets through a process called liquid phase separation. (neurosciencenews.com)
Protein-rich droplets that formed through a process called liquid phase separation fuse and grow under the microscope. (neurosciencenews.com)
When hnRNPA1 with mutations in the disordered tail underwent liquid phase separation and concentrated into droplets, the protein almost immediately formed amyloid-like fibrils. (neurosciencenews.com)
If the mutant protein did not concentrate into droplets, however, toxic fibrils did not form. (neurosciencenews.com)
Essentially, these proteins separate from the cellular liquid to form a second liquid phase at the microtubule's tip, like oil droplets in water.This phase separation phenomenon significantly increases the concentration of certain proteins, including tubulin, which in turn, stimulate the growth rate of microtubules, while reducing their breakdown. (expertmarketresearch.com)

Optimal Aqueous Biphasic Systems Design for the Recovery of Ionic Liquids. (acs.org)
Effect of Salt Type and Alkyl Chain Length on the Binodal Curve of an Aqueous Two-Phase System Composed of Imidazolium Ionic Liquids. (acs.org)

A large pore is formed by transmembrane proteins. (tcdb.org)
This new approach has been shown to preserve mobility and enzymatic activity of transmembrane proteins in the resulting nSLB. (lu.se)

We are working on three areas of research: 1) Biomolecular Electrostatics , 2) Protein-DNA Dynamics , and 3) HMGB1 Biochemistry . (utmb.edu)

2011) addresses two of these aspects, the translocation of soluble proteins into the peroxisomal matrix and the biogenesis of the peroxisomal membrane. (tcdb.org)
Most peroxisomal membrane proteins do not have PTS1 or PTS2, and insertion requires several peroxins distinct from those required for import of soluble matrix proteins. (tcdb.org)
The proteins are made soluble in a buffer solution. (euronews.com)
Silks are composed principally of proteins with a predominance of alanine, serine and glycine and silk proteins are able to undergo irreversible transformations from soluble protein to insoluble fibres. (bioone.org)

2022). Sexually dimorphic RNA helicases DDX3X and DDX3Y differentially regulate RNA metabolism through phase separation. (upenn.edu)

Coacervate / condensate phase of polyelectrolytes and proteins. (aalto.fi)
Using coarse-grain simulations (1) and biochemical reconstitution, our team identified intrinsically disordered proteins that do not mix with the Laf-1 RGG polypeptide and instead form distinct mesoscale condensate materials. (upenn.edu)
Additionally, our team has developed surface active agents built from protein blocks that localize to condensate interfaces and enable tunable control of condensate size. (upenn.edu)
The protective ApoE4 isoform lacks these cysteines and is resistant to phase separation and condensate formation. (jci.org)

Our results highlight a need for further research into how misfolded protein aggregates disrupt the histone PTM landscape. (cuny.edu)

Fast reversed-phase liquid chromatographic separation of proteins by flow-through poly(styrene-co-divinylbenzene) microspheres. (bvsalud.org)
1986. Liquid and gas chromatographic determination of 1,3,5trinitrobenzene in water. (cdc.gov)

Their flexibility and ability for multiple weak protein-protein interactions (PPIs) seem to be crucial to form a protein-rich separated phase. (spp2191.com)
Our research field is biophysical chemistry of proteins, DNA, and their interactions. (utmb.edu)
By harnessing liquid-liquid phase separation and leveraging supramolecular interactions between chimeric protein and natural DNA, the researchers achieved a reinforced adhesion performance in the biocomposite adhesive. (news-medical.net)
Applications of these models at a near-atomic resolution further revealed physicochemical interactions that drive the phase separation of disordered proteins and dictate chromatin stability in situ. (nyu.edu)
yet, the counterintuitive notion that particles with no interactions other than excluded volume might self-assemble from a fluid phase into an ordered crystal has been known since the mid-20th century. (ucsb.edu)
This article is part of a Special Issue entitled: Oxidized phospholipids - their properties and interactions with proteins. (cdc.gov)

Eph receptors and their ligands, Ephrin proteins (Eph receptor interacting proteins), play a key role in many pathological states (abnormally elevated RTK activity is a feature of most human cancers), and therefore Eph receptors can be used as potential drug targets [ 9 , 10 ]. (aging-us.com)

Intrinsically disordered proteins (IDPs) play a central role in desiccation tolerance in all species investigated. (researchgate.net)
Around 30% of all proteins in eukaryotic organisms have recently been shown to belong to intrinsically disordered proteins (IDPs) involved in many central biological processes and diseases. (lu.se)
To reveal static and dynamic properties of intrinsically disordered proteins (IDPs) to provide a deeper understanding of the mechanism of action and the static as well as dynamic structure-function relationship. (lu.se)

Liquid-liquid phase separation of nucleocapsid proteins during SARS-CoV-2 and HIV-1 replication. (cdc.gov)

Inflammatory cytokines and growth factors via RSK AKT and Protein Kinase A (PKA) induce phosphorylation of EphA2 Ser897. (aging-us.com)
The ability of the acetate-based salts to induce the phase separation not only depends on the ions hydration energy, but also on the concentration of free ions in solution. (ua.pt)

The new electrostatic methods are applicable to various processes, including macromolecular association and liquid-liquid phase separation. (utmb.edu)
Liquid-liquid phase separation, driven by electrostatic complexation between a chimeric epidermal growth factor (EGF), elastin-like protein, and natural DNA, facilitated the assembly of the adhesive. (news-medical.net)

Native membrane derived polymer-supported lipid bilayers (nSLBs) are poised to bridge the gap between live cell experiments and traditional model membrane architectures that by offering a combination of accessibility by surface sensitive analytical instrumentation and a composition which more closely resembles cellular membranes by displaying a diversity of endogenous membrane proteins, lipids, and carbohydrates. (lu.se)

The long disordered tails in these proteins enable membrane-less compartmentalization in cells. (neurosciencenews.com)

This involves protein extraction from the biological sample of interest, followed by protein denaturation to break down the tertiary structure and expose the amino acids. (pharmiweb.com)
2005). Briefly, without protein precipitation, only dilution with 0.1 M formic acid, one aliquot of 100 μL of serum was injected into a commercial column switching system allowing for concentration of the analytes on a C18 solid-phase extraction column. (cdc.gov)
MALDI analysis of proteins after extraction from dissolvable ethylene glycol diacrylate cross-linked polyacrylamide gels. (uni-frankfurt.de)
Ionic-liquid-based aqueous biphasic systems (IL-based ABS) have been largely investigated as promising extraction and purification routes. (ua.pt)

The contributors discuss the 3D organization of chromatin, the various nuclear bodies and compartments that have been identified, and the roles of RNA and actin in shaping nuclear organization, as well as how these structures interact with each other and with peripheral features (e.g., the nuclear pore complex and inner nuclear membrane proteins) to carry out the work of the nucleus. (cshlpress.com)

It is amazing to find out that proteins like hnRNPA1 have appeared in nature to mediate liquid phase separation under normal physiological conditions," Mittag said. (neurosciencenews.com)

The membrane bound docking subcomplex includes several Pex proteins, Pex13p, 14p and 17p. (tcdb.org)
While many studies have been focused on the membrane structure, the dynamics of such systems are crucial for the function of the membrane including membrane bound proteins. (lu.se)

We believe that by studying the interface between neurodegeneration, protein misfolding, and epigenetics, we can elucidate the mechanisms leading to disease, and potentially reveal novel targets for therapeutic development. (cuny.edu)

It can very well be a state with higher energy than some intermediates, giving the protein intrinsic dynamic flexibility or induced dynamic response to stimuli. (biophysics.org)

These databases contain manually curated data supported by experimental evidence in the literature and can include related features as presence of protein disorder, low complexity, post-translational modifications, experimental details, phase diagrams, among others. (wikipedia.org)
In this context, the determination of their phase diagrams and the physical properties of the coexisting phases are of high relevance when envisaging their large-scale applications. (ua.pt)

Our studies demonstrate that major AMD genetic and biological risk pathways converge upon RPE mitochondria, and identify mitochondrial stress-mediated protein phase separation as an important pathogenic mechanism and promising therapeutic target in AMD. (jci.org)

These same proteins accumulate in thread-like deposits called amyloid fibrils in the nerve and muscle cells of patients with ALS, frontotemporal dementia (FTD) and inclusion body myopathy (IBM). (neurosciencenews.com)
Membrane phase separation into liquid-ordered and liquid-disordered domains has been speculated to play a role in controlling the cleavage of amyloid precursor protein (APP) by the α-secretase ADAM10 (a disintegrin and metalloproteinase domain-containing protein 10) and the β-secretase BACE1 (𝛽-site amyloid precursor protein cleaving enzyme 1), affecting the formation of the amyloid-β peptides, oligomers, and fibrils implicated in Alzheimer's disease. (biophysics.org)

Proper protein folding is a delicate balance that is crucial for normal biological function. (cuny.edu)
In addition, liquid phase separation is probably important for a whole range of fundamental biological processes. (neurosciencenews.com)

During this time, he developed a strong expertise on protein folding stability and hydration dynamics of biomolecules in the test tube, using fluorescence and terahertz spectroscopy. (spp2191.com)
This newly discovered mechanism appears to play a concrete role in controlling the dynamics of cellular microtubules.The synergy between CLIP-170 and EB3 is crucial for regulating microtubule growth, owing to this phase separation mechanism. (expertmarketresearch.com)

2011 ) have reviewed the processes by which matrix and membrane proteins are incorporated into the peroxysome. (tcdb.org)

The hydrolysis process breaks the peptide bonds, converting proteins into their constituent amino acids. (pharmiweb.com)
The constituent proteins are normally synthesised in specialised glands where the epithelial cells are responsible for the biosynthesis. (bioone.org)

It is here shown that low-viscous IL-based ABS, with a high difference in the densities of the coexisting phases, can be formed with organic and biodegradable salts thus offering enhanced features over conventional polymer-based systems. (ua.pt)

Protein amino acid analysis is a technique for determining the type and amounts of amino acids in a protein sample, providing information on protein composition, post-translational modifications and overall protein quality. (pharmiweb.com)
By gaining insight into amino acid content, researchers can assess protein purity, determine changes in amino acid composition, and evaluate protein stability. (pharmiweb.com)
What is Protein Amino Acid Analysis? (pharmiweb.com)
Automated instruments specifically designed for protein amino acid analysis. (pharmiweb.com)
Combining mass spectrometry with separation techniques such as HPLC or CE can improve the accuracy and sensitivity of amino acid analysis. (pharmiweb.com)
A reversed-phase column is commonly used for amino acid analysis. (pharmiweb.com)
hnRNPA1 belongs to a subset of proteins with an amino acid arrangement that prevents folding of one end of the protein, which allows hnRNPA1 to adopt a variety of conformations. (neurosciencenews.com)

The peroxisomal protein import machinery, which shares similarities with chloroplasts, is unique in transporting folded and large (up to 10 nm in diameter) protein complexes into peroxisomes. (tcdb.org)

Group 1 (Dur-19, PF00477, LEA_5) Late Embryogenesis Abundant (LEA) proteins are present in organisms from all three domains of life, Archaea, Bacteria, and Eukarya. (researchgate.net)

IEC, HPLC or CE) and detection methods described above to identify and quantify amino acids in protein samples. (pharmiweb.com)

The majority of proteins function as monodispersed ordered species dissolved in intra- or extra-cellular aqueous fluids. (frontiersin.org)

In Abca-/- Stargardt macular degeneration mice, mitochondrial dysfunction induces liquid-liquid phase separation of p62/SQSTM1, a multifunctional protein that regulates autophagy. (jci.org)
The specific functions of mitoNEET/CISD1 remain to be fully elucidated, but the protein is involved in regulating mitochondrial bioenergetics in several metabolic diseases. (researchgate.net)

In our recent study, it was revealed that PXR interacts with DAZ-associated protein 1 (DAZAP1), which is an essential component of the paraspeckle, a membraneless nuclear body, and the interaction was disassociated by rifampicin, a ligand of PXR. (aspetjournals.org)

C-terminal PTS1 [(SAC)-(KRH)-(LM)] and N-terminal PTS2 [(RK)-(LIV)-X 5 -(HQ)-(LA)] targeting sequences are recognized by Pex5p and Pex7p, respectively, which may shuttle with the substrate protein to the peroxisomal lumen. (tcdb.org)
The proteins are subsequently secreted into the glandular lumen where they are stored until extrusion. (bioone.org)

Insulin receptor substrate (IRS) proteins play essential roles in mediating insulin/IGF signaling by recruiting a series of downstream effectors 23 . (nature.com)
In the budding yeast Saccharomyces cerevisiae , the minimal transport machinery includes the membrane proteins Pex13 and Pex14 and the cargo-protein-binding transport receptor, Pex5. (tcdb.org)

While it is undisputed that late embryogenesis abundant (LEA) proteins play a key role in the tolerance of plants and. (researchgate.net)

Both join in Simon´s lab in 2018 for Post-Doctorate and Doctorate, respectively, eager to investigate the connection between protein conformational changes and liquid-liquid phase separation inside living cells. (spp2191.com)
We developed NMR methods to quantitatively investigate counterions around proteins and DNA.Our NMR methods were successfully applied to 15 NH 4 + , 23 Na + , 7 Li + , 133 Cs + , and 13 C acetate (OAc - ) ions around DNA and proteins. (utmb.edu)
The aim is to understand how the lipid environment influences the activity of membrane proteins, using the active transporter MgtA as benchmark system, and to investigate the dynamic fluctuations between lipid and protein in solution, utilising neutron and X-ray scattering in combination with computer simulations. (lu.se)

This phenomenon of phase separation, at the microtubule level, increases the concentration of proteins, including tubulin, and significantly stimulates the growth rate of microtubules while reducing depolymerization events, i.e., microtubule decay events. (expertmarketresearch.com)

Over two dozen proteins involved in protein import and membrane insertion, peroxins, encoded by PEX genes, have been characterized. (tcdb.org)
The reports on base sequences of spider silk protein genes have gained importance as the mechanical properties of silk fibers have been revealed. (bioone.org)
Genes encoding the protein components of stress granules are often mutated in patients with ALS and other diseases. (neurosciencenews.com)

Adsorption of plasma proteins on uncoated PLGA nanoparticles. (uni-frankfurt.de)

Since these intrinsically disordered regions, which are conformationally dynamic and do not adopt stable secondary or tertiary structures, are often essential for mediating the phase transition of proteins 9 , it is therefore of interest to consider if the C-terminus of IRS-1 is involved in phase separation and to further delineate such implications upon insulin/IGF signaling. (nature.com)
As the optical transition dipole in TQ1 is parallel to the polymer backbone, the phase orientations mark local polmer chain orientations. (lu.se)

This mechanism involves two specific proteins, CLIP-170 and EB3, undergoing a process called liquid-liquid phase separation at the tip of the microtubules during their growth. (expertmarketresearch.com)

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20212

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