• FPLC was developed and marketed in Sweden by Pharmacia in 1982, and was originally called fast performance liquid chromatography to contrast it with high-performance liquid chromatography (HPLC). (wikipedia.org)
  • Asynt has introduced GasTrap gas purifiers for analytical laboratories using Gas Chromatography (GC), High Performance Liquid Chromatography (HPLC) or Mass Spectrometry (GC/MS) instrumentation. (news-medical.net)
  • This protein profiling method using gel electrophoresis coupled with nano-HPLC/ESI/MS/MS and manual evaluation is a sensitive and accurate method for protein identification as well as a powerful tool for monitoring various types of food products. (elsevierpure.com)
  • A straightforward and sensitive reversed-phase high-performance liquid chromatography (HPLC) assay was developed and validated for the analysis of osthol and its phase I metabolites (internal standard: umbelliferone). (ampkpathway.com)
  • We used consecutive orthogonal separation platforms to ensure sensitive detection: (a) ion-exchange of intact proteins, (b) SDS-PAGE separation of ion-exchange fractions and (c) HPLC separation of tryptic digests coupled to electrospray tandem mass spectrometry. (hindawi.com)
  • A theoretical description of analyte retention in high-performance liquid chromatography (HPLC) has been the subject of various publications. (springer.com)
  • Hydrophilic interaction liquid chromatography (HILIC) is an alternative high-performance liquid chromatography (HPLC) mode for separating polar compounds. (springer.com)
  • Chaudhary-Webb M, Schleicher RL, Erhardt JG, Pendergrast EC, Pfeiffer CM. An HPLC ultraviolet method using low sample volume and protein precipitation for the measurement of retinol in human serum suitable for laboratories in low- and middle-income countries external icon . (cdc.gov)
  • We used high-performance liquid chromatography (HPLC) to measure the levels of DA and its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in the dialysis perfusates. (lu.se)
  • Fast protein liquid chromatography (FPLC) is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. (wikipedia.org)
  • FPLC can be readily scaled from analysis of milligrams of mixtures in columns with a total volume of 5 ml or less to industrial production of kilograms of purified protein in columns with volumes of many liters. (wikipedia.org)
  • In a common FPLC strategy, a resin is chosen that the protein of interest will bind to by a charge interaction while in buffer A (the running buffer) but become dissociated and return to solution in buffer B (the elution buffer). (wikipedia.org)
  • Strep -tagged proteins can be purified using batch or gravity-flow methods, or FPLC-compatible columns can be filled with Strep -Tactin Superflow Plus for purification on liquid chromatography systems. (qiagen.com)
  • Strep -Tactin Superflow Plus is available in 5 ml cartridges for automated purification on liquid chromatography systems such as the FPLC, ÄkTA, and BioLogic systems, or manual purification using a syringe. (qiagen.com)
  • We established an efficient approach, crosslinking of segmentally isotope-labeled RNA and tandem mass spectrometry (CLIR-MS/MS), to localize protein-RNA interactions simultaneously at amino acid and nucleotide resolution. (nih.gov)
  • Hong, SS, Park, JH & Kwon, SW 2007, ' Determination of proteins in infant formula by high-performance liquid chromatography-electrospray tandem mass spectrometry ', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , vol. 845, no. 1, pp. 69-73. (elsevierpure.com)
  • Immunostained P. variotii antigens were excised and sequenced using nanoscale Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry (nano scale UPLC-MS/MS). De-identified sera from 6 healthcare workers (HCW) and 2 non-atopics were used as controls. (cdc.gov)
  • By using our newly developed liquid chromatography-tandem mass spectrometry quantification method, we were able to measure the absolute amount of MRP2/Mrp2 in liver tissues and isolated hepatocytes across species. (aspetjournals.org)
  • This method required 10 microliters of serum and utilizes liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and isotope dilution for quantification. (cdc.gov)
  • Samples were then separated via liquid chromatography and detected via tandem mass spectrometry as previously reported (Pantazides et. (cdc.gov)
  • The researchers used liquid chromatography with tandem mass spectrometry analysis to fully profile the serum of healthy donors and patients with TNBC. (asbmb.org)
  • They analyzed protein interactions of each using liquid chromatography with tandem mass spectrometry. (asbmb.org)
  • Chen H, McCoy LF, Schleicher RL, Pfeiffer CM. Measurement of 25-hydroxyvitamin D3 (25OHD3) and 25-hydroxyvitamin D2 (25OHD2) in human serum using liquid chromatography-tandem mass spectrometry and its comparison to a radioimmunoassay method external icon . (cdc.gov)
  • Fazili Zand Pfeiffer CM. Accounting for an isobaric interference allows correct determination of folate vitamers in serum by isotope dilution-liquid chromatography-tandem mass spectrometry external icon . (cdc.gov)
  • Fazili Z, Pfeiffer CM, Zhang M, Jain R. Erythrocyte folate extraction and quantitative determination by liquid chromatography-tandem mass spectrometry: comparison of results with microbiologic assay external icon . (cdc.gov)
  • McCoy LF, Scholl PF, Schleicher RL, Groopman JD, Powers CD, Pfeiffer CM. Analysis of aflatoxin B1-lysine adduct in serum using isotope-dilution liquid chromatography/tandem mass spectrometry external icon . (cdc.gov)
  • Mineva EM, Schleicher RL, Chaudhary-Webb, M, May KL, Botelho JC, Vesper HW, Pfeiffer CM. A candidate reference measurement procedure for quantifying serum concentrations of 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 using isotope-dilution liquid chromatography-tandem mass spectrometry external icon . (cdc.gov)
  • Saliva was analyzed by two-dimensional liquid chromatography and tandem mass spectrometry for proteomic identification of salivary proteins associated with oral Candidiasis. (scienceblog.com)
  • Patient plasma samples were analyzed using label-free data-independent acquisition liquid chromatography tandem mass spectrometry. (lu.se)
  • The researchers used the gold standard method, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), to measure blood levels of three AGEs: N ε -(carboxy-methyl)lysine (CML), N ε -(carboxy-ethyl)lysine (CEL), and N δ -(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine (MG-H1). (who.int)
  • However, because of the unknown binding affinity of antibody to MRP2/Mrp2 protein across species and lack of purified MRP2/Mrp2 proteins for calibration, the immunoblotting assay was excluded from the absolute quantification of MRP2/Mrp2 protein for multiple species. (aspetjournals.org)
  • In this study we developed an orthogonal immunoprecipitation - liquid chromatography - triple quadrupole mass spectrometry (IP-LC-TQMS) assay to verify the observed reductions in total p217+ tau levels. (quanterix.com)
  • Strep- Tactin Superflow Plus allows highly efficient purification of proteins (see figures Efficient purification of Strep -tagged GFP. GFP was expressed in 60 ml E. coli culture and a 5 ml aliquot was applied to Strep -Tactin Superflow Plus. Protein was eluted using 2.5 mM desthiobiotin, giving a total protein yield of 3.1mg. C: Cleared lysate; E: Elution fractions; F: Flow-through; M: Markers; W: Wash. "> Efficient purification of Strep-tagged GFP with Strep-Tactin Superflow Plus and Highly specific detection of Strep-tagged proteins in any expression system. Purified Strep -tagged thioredoxin (10 ng) was mixed with crude cell lysate from mammalian (NIH-3T3), insect (Sf9), and bacterial ( E. coli ) cells (each 10 µg total protein). After electrophoresis and western transfer, the Strep -tagged protein was detected using the Strep -tag Antibody and chemiluminescent detection. "> Highly specific detection of Strep-tagged proteins in any expression system ). (qiagen.com)
  • Utilizing mechanisms including normal phase, reversed phase, ion-exchange, and affinity chromatography and can be combined for mixed-mode chromatography applications. (coleparmer.com)
  • Meanwhile, pQE TriSystem His· Strep TriSystem Vectors can be used to express proteins doubly-tagged with a His- and a Strep -tag, which are purified in a two-step affinity procedure. (qiagen.com)
  • Strep- tagged proteins bind with high affinity and specificity to Strep- Tactin, an engineered form of streptavidin (see table Protein binding capacities of Strep- Tactin matrices). (qiagen.com)
  • Strep -tagged proteins are expressed using the pQE TriSystem Vector and obtained at very high purity from cell lysates using Strep -Tactin Superflow Plus affinity purification procedures. (qiagen.com)
  • in others, the target protein is expressed from a plasmid that encodes for an "affinity tag" specific to a particular ligand. (the-scientist.com)
  • Previous proteomic studies of intact platelets have collectively identified hundreds of proteins using a variety of fractionation strategies including 2-dimensional electrophoresis (2DE), multidimensional chromatographic separations, membrane prefractionation techniques, and adsorption to combinatorial hexapeptide ligand libraries [ 1 - 6 ]. (hindawi.com)
  • The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts-a critical and often neglected step in successful protein purification. (cshlpress.com)
  • Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. (cshlpress.com)
  • In the first dimension, ion-exchange chromatography (IEC) separates peptides based on ionic interactions with the solid phase and an increasing salt buffer. (hindawi.com)
  • Selective trapping of diphospho-peptides via a metal oxide chromatography mechanism was achieved in a first dimension with a conventional reversed phase stationary phase and acidified mobile phase. (quanterix.com)
  • 2016. Isolation and identification of antioxidant peptides from enzymatically hydrolyzed rice bran protein. . (oregonstate.edu)
  • The issue of abundant (often housekeeping) proteins masking regulatory proteins of lower abundance (such as signaling proteins and cytokines) continues to be a challenging issue for proteomics particularly in the case of biofluids. (hindawi.com)
  • While modern proteomics experiments permit the analysis of hundreds to thousands of proteins in complex samples, the most powerful use of this data would combine information on protein activity with the identities of the active proteins. (hindawi.com)
  • A new study in the journal Molecular & Cellular Proteomics characterizes the functions of CD151, a protein that researchers have found to be significant in TNBC. (asbmb.org)
  • PKC, protein kinase C. (aspetjournals.org)
  • Its activation is tightly controlled by numerous other signaling proteins including protein kinase C (PKC), Sab/SH3BP, and caveolin-1. (medscape.com)
  • A previous study from our laboratory using a MuDPIT (multidimensional protein identification technology) approach identified over 300 proteins secreted by platelets upon thrombin activation [ 1 ]. (hindawi.com)
  • They found increased expression levels of ITGB1, a known prognostic marker for cancer, and the protein CD151 in TNBC serum samples. (asbmb.org)
  • An important factor is that, with protein deficiency, serum vitamin A levels may be decreased despite good vitamin A intake and adequate vitamin A stores. (medscape.com)
  • Total and holo retinol-binding protein (RBP) test: These tests (complex of vitamin A and RBP) for serum RBP tend to correlate with measures of serum vitamin A. These levels can also be decreased in the presence of protein deficiency. (medscape.com)
  • Esmaeili R, Zhang M, Sternberg MR, Mapango C, Pfeiffer CM . A new multiplex immunoassay for serum ferritin, soluble transferrin receptor, C-reactive protein, α-1-glycoprotein, and retinol-binding protein showed variable comparability with traditional reference assays external icon . (cdc.gov)
  • Pawlosky RJ, Flanagan VP, Pfeiffer CM. Determination of 5-methyltetrahydrofolic acid in human serum by stable-isotope dilution high-performance liquid chromatography-mass spectrometry external icon . (cdc.gov)
  • The small Strep- tag II (WSHPQFEK) can be used to purify active, native conformation proteins to high purity on Strep- Tactin resins. (qiagen.com)
  • There are two major P450 classes in terms of the native RP systems, namely, the prokaryotic Class I P450 consisting of three stand-alone components (redoxin reductase/redoxin/P450) that are all cytosolically soluble proteins and the two-component eukaryotic Class II P450 comprised of P450 and cytochrome P450 reductase (CPR), both of which are membrane-bound proteins. (nature.com)
  • Following activation by agonists such as thrombin, platelets release storage granules and membrane vesicles that contain prothrombotic (e.g., fibrinogen), mitogenic (e.g., platelet derived growth factor), immunomodulatory (e.g., neutrophil-activating peptide 2), and adhesive (e.g., platelet endothelial cell adhesion molecule) proteins. (hindawi.com)
  • Protein and peptide chromatography prior to MS analysis can partly address this issue. (hindawi.com)
  • Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
  • Anti-PnMas1 and anti-PnMas2 antibodies co-labelled two high-molecular-weight (~400 kDa) protein complexes in native gels but anti-PnMas3 antibodies labelled a 65 kDa protein complex. (springer.com)
  • The proteins of interest bind to the resin while other components are carried out in the buffer. (wikipedia.org)
  • A glass frit at the bottom of the column retains the resin beads in the column while allowing the buffer and dissolved proteins to exit. (wikipedia.org)
  • Strep- Tactin is available as Superflow chromatography resin for larger scale purification (up to 9 mg/ml resin). (qiagen.com)
  • Strep- Tactin resin is also available in prefilled cartridges for automated purification on liquid chromatography systems. (qiagen.com)
  • But liquid chromatography frequently requires a considerable amount of optimization, and usually involves several different chromatographic steps to rid the sample of contaminants.1 The ideal solution would be to create a resin that is completely specific to the target protein, enabling one-step purification. (the-scientist.com)
  • 1 The ideal solution would be to create a resin that is completely specific to the target protein, enabling one-step purification. (the-scientist.com)
  • The in vitro α-glucosidase inhibitory activity was analyzed using all the fractions collected, followed by profiling of the metabolites using liquid chromatography combined with mass spectrometry. (upm.edu.my)
  • The aim of this work was to characterize the composition of these seeds to assess not only their nutritional potential (protein, lipid and minerals content), but also their antioxidant and anti-inflammatory potential as well as their specialized metabolites composition. (ac.be)
  • 1992. Quantification of 4,4´-diaminodiphenylmethane by gas chromatography-negative-ion chemical-ionization mass spectrometry. (cdc.gov)
  • As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the mobile phase) and a porous solid (the stationary phase). (wikipedia.org)
  • In normal phase liquid chromatography (NP-LC), the stationary phase is more polar than the mobile phase. (springer.com)
  • NP-LC has been widely used to separate various compounds, from nonpolar to highly polar compounds (note that chromatography was first introduced as a method used in separation science). (springer.com)
  • For historical reasons, it has been reported that HILIC is a variant of normal phase liquid chromatography, but the separation mechanism used in HILIC is more complicated than that in NP-LC. (springer.com)
  • Figure 2 shows how HILIC complements other areas of chromatography and extends the range of separation options. (springer.com)
  • Hydrophilic interaction liquid chromatography has established itself as the separation mode of choice for uncharged highly hydrophilic and amphiphilic compounds that are too polar to be well retained in RP-LC but have insufficient charge to allow effective electrostatic retention in ion-exchange chromatography. (springer.com)
  • In the present study, the ADE without solid-liquid separation was mixed with sewage sludge and Chinese medicine residue for the composting, where the ADE could be recovered at high temperature through humification. (bvsalud.org)
  • The in silico study performed via molecular docking with the crystal structure of yeast isomaltase (PDB code: 3A4A) involved a hydrogen bond and some hydrophobic interactions between the inhibitors and protein. (upm.edu.my)
  • For instance, a number of studies have used 1D SDS-PAGE to separate proteins by molecular weight prior to MS analysis [ 12 - 15 ]. (hindawi.com)
  • One of the key molecular events in skin sensitization is protein haptenation, i.e. the chemical modification of self-skin protein(s) thus forming macromolecular immunogens. (cdc.gov)
  • The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)
  • The cross-reactions of MRP2/Mrp2 protein of different species with anti-human MRP2 polyclonal antibody were found by Western blotting. (aspetjournals.org)
  • People have been able to detect a handful of proteins in individual cells using fluorescence- or antibody-based methods. (acs.org)
  • Antibody is purified by protein A chromatography method. (prosci-inc.com)
  • They also believe it may be important to study variations in protein interactions among emerging SARS-CoV-2 variants. (asbmb.org)
  • The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. (cshlpress.com)
  • Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. (cshlpress.com)
  • Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. (springer.com)
  • Proteomic approaches have proven powerful at identifying large numbers of proteins, but there are fewer reports of functional characterization of proteins in biological tissues. (hindawi.com)
  • Six extracellular protein genes up-regulated in stomatitis were confirmed within the saliva using proteomic methods. (scienceblog.com)
  • 1986. Methods validation study of high performance liquid chromatographic technique for determining the MPDA and MDA content of air samples. (cdc.gov)
  • After digestion, any undigested proteins were removed by protein precipitation. (cdc.gov)
  • 1990. Monitoring exposure to 4,4´-methylenedianiline by the gas chromatography-mass spectrometry determination of adducts to hemoglobin. (cdc.gov)
  • For purification of Strep -tagged proteins: 10 ml Strep -Tactin-charged Superflow Plus suspension (50%) containing 0.02% sodium azide (max. (qiagen.com)
  • Using an integrated platform of metabolic labeling, high-resolution mass spectrometry and computational analysis, we report here a comprehensive dataset of the in vivo half-life of 3,228 and the expression of 8,064 cardiac proteins, quantified under healthy and hypertrophic conditions across six mouse genetic strains commonly employed in biomedical research. (nature.com)
  • Which type of chromatography is best for cannabis analysis? (news-medical.net)
  • Chromatography sample preparation is a necessary step for chromatography analysis. (coleparmer.com)
  • Based on immunological and mass spectrometric analysis, SALSA was estimated to constitute up to 4-10% of the total protein amount in meconium, making it one of the most abundant proteins. (researchgate.net)
  • Normal or reversed-phase liquid chromatography can be used for analysis. (springer.com)
  • HILIC also allows the analysis of charged substances, as in ion chromatography (IC). (springer.com)
  • Liquid chromatography-mass spectrometry analysis identified PnMas1 and PnMas2 but not PnMas3 in flagellar extracts. (springer.com)
  • The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. (cshlpress.com)
  • Simultaneous determination of amines and isocyanates in working atmospheres by gas-liquid chromatography. (cdc.gov)
  • Determination of 4,4´-methylenedianiline m hydrolyzed human urine by micro liquid chromatography with ultraviolet detection. (cdc.gov)
  • These immundominant allergens were identified by nanoscale UPLC-MS/MS to be 60S ribosomal protein L10 (25 kDa), electron transfer flavoprotein B subunit (29 kDa), ketol acid reductoisomerase (44 kDa), and heat shock protein 70 (65 kDa). (cdc.gov)
  • In addition to the blood pressure criteria, proteinuria of greater than or equal to 0.3 grams in a 24-hour urine specimen, a protein (mg/dL)/creatinine (mg/dL) ratio of 0.3 or higher, or a urine dipstick protein of 1+ (if a quantitative measurement is unavailable) is required to diagnose preeclampsia. (medscape.com)
  • Complex biological samples have also been successfully separated in multiple dimensions at the protein level. (hindawi.com)
  • SALSA proteins in the AF and intestinal samples were polymorphic and exhibited varying polypeptide compositions. (researchgate.net)
  • Briefly, calibrators, quality controls, a matrix blank, blind QCs, and NHANES samples were prepared by performing a protein digestion using pronase to release the chlorinated tyrosine biomarkers. (cdc.gov)
  • The eluant passes through two detectors which measure salt concentration (by conductivity) and protein concentration (by absorption of ultraviolet light at a wavelength of 280nm). (wikipedia.org)
  • A number of bleeding disorders arise as a result of mutations in the genes for proteins involved in platelet aggregation. (hindawi.com)
  • In the current study, genes encoding two related proteins, PnMas1 and PnMas3, were identified in the genome of P. parasitica . (springer.com)
  • As each protein is eluted, it appears in the eluant as a "peak" in protein concentration, and can be collected for further use. (wikipedia.org)
  • The eluant from the column passes through one or more flow cells to measure the concentration of protein in the eluant (by UV light absorption at 280 nm). (wikipedia.org)
  • Plasma has a significant dynamic range, with more than 10 orders of magnitude separating albumin concentration and the rarest measurable proteins identified to date [ 10 ]. (hindawi.com)
  • We demonstrated promising results for the use of an integrated EBP biomarker panel together with particle concentration for diagnosis of COVID-19 as well as a robust method for protein identification in EBPs. (lu.se)
  • A His -or Strep-tag II can be added to either terminus, greatly simplifying protein purification and detection after expression. (qiagen.com)
  • Proteins identified within the fraction with monocyte promigratory activity and not in other inactive fractions included vimentin, PEDF, and TIMP-1. (hindawi.com)
  • Proteins with quantified dynamics belong to over 10 major cellular compartments and over 200 known pathways. (nature.com)
  • Key proteins in mitochondria and metabolic pathways are encompassed, in addition to contractile machineries and sarcolemmal signaling proteins. (nature.com)
  • Conclusion Proteins involved in several thrombogenic pathways were differentially expressed in Lemierre's syndrome compared to other severe infections. (lu.se)
  • Nsp3, a large multidomain protein, can enzymatically break down other proteins, perform cleavage reactions on itself and perform other important regulatory reactions. (asbmb.org)
  • The three AGEs measured are among the most abundant in the body, and the researchers specifically measured the protein-bound fraction, because this form of AGEs is known to promote inflammation in colon tissues. (who.int)
  • Freshly isolated hepatocytes conserved MRP2/Mrp2 protein levels that are comparable with those in the liver tissues. (aspetjournals.org)
  • Blackman LM, Arikawa M, Yamada S, Suzaki T, Hardham AR (2011) Identification of a mastigoneme protein from Phytophthora nicotianae . (springer.com)
  • Protein stability is a major regulatory principle of protein function and cellular homeostasis. (nature.com)
  • In contrast to steady-state protein abundance, which may be quantified directly in mass spectrometry (MS) by spectral intensity 16 , 17 or sampling frequency 18 , 19 , protein turnover rates cannot be predicted from steady-state data 20 , requiring instead methods that can distinguish old and new protein molecules in mass spectra 11 , 21 . (nature.com)
  • CD151 is a scaffolding protein that interacts with integrin receptors. (asbmb.org)
  • The team intends to investigate how nsp3 interacts with two other nonstructural proteins known to coordinate to form double-membrane vesicles out of the ER to replicate coronaviruses. (asbmb.org)
  • Detailed protocols involving protein microarrays, liquid chromatography, high throughput cloning of expression constructs, IMAC, mass spectrometry, MALDI TOF, and MudPIT are provided, along with well illustrated descriptions of experimental procedures and lists of recommended Web sites and reading material. (cshlpress.com)
  • The history of protein dynamics traces back to 1935, when Schoenheimer and Rittenberg synthesized the first isotopologs of biological molecules to demonstrate the continuous renewal of proteins throughout life 14 , 15 . (nature.com)
  • Understanding how proteins function is an essential part of many biological research endeavors. (cshlpress.com)
  • After washing, proteins are eluted using low concentrations of biotin (the natural ligand of streptavidin) or desthiobiotin (a stable, reversibly binding biotin analog, see figure Binding of Strep-tagged proteins to Strep-Tactin and elution using desthiobiotin. "> Binding of Strep-tagged proteins to Strep-Tactin and elution using desthiobiotin ). (qiagen.com)
  • Over 300 proteins were identified in the releasate, with a wide range of annotated biophysical and biochemical properties, in particular platelet activation, adhesion, and wound healing. (hindawi.com)
  • The presence of PEDF and involucrin, two proteins not previously reported in platelet releasate, was confirmed by western blotting. (hindawi.com)
  • Among identified proteins, several were associated with endothelial damage, platelet activation and degranulation and warrant further targeted studies. (lu.se)
  • We achieved a deep proteome profiling of EBP across the three groups with proteins involved in immune activation, acute phase response, cell adhesion, blood coagulation, and known components of the respiratory tract lining fluid, among others. (lu.se)
  • Her main research interests are bovine and equine inflammatory disease and acute phase proteins, the surgical stress response, joint disease and wound healing. (ku.dk)
  • Because of its favorable safety profile and the involvement of misfolded proteins, oxidative damage, and inflammation in multiple chronic degenerative diseases, these data relating curcumin dosing to the blood and tissue levels required for efficacy should help translation efforts from multiple successful preclinical models. (aspetjournals.org)
  • Enhancing our understanding of retention behavior in HILIC increases the scope of possible applications of liquid chromatography. (springer.com)