• Influenza D virus M2 protein exhibits ion channel activity in Xenopus laevis oocytes. (cdc.gov)
  • Xenopus laevis oocytes expressing hOAT1 supported probenecid-sensitive uptake of [ 3 H] p -aminohippurate ( K m = 4 μM), which was trans -stimulated in oocytes preloaded with glutarate. (aspetjournals.org)
  • Expression of Channelopsin-1, or only the hydrophobic core, in Xenopus laevis oocytes in the presence of all-trans retinal produces a light-gated conductance that shows characteristics of a channel selectively permeable for protons. (scienceopen.com)
  • Mitogen-activated protein kinase (MAPK) and MAPK kinase (MAPKK) are activated during Xenopus oocyte maturation concomitant with the activation of maturation promoting factor (MPF). (elsevierpure.com)
  • We reported previously that an anti-MAPKK neutralizing antibody inhibited progesterone- or Mos- induced initiation of oocyte maturation. (elsevierpure.com)
  • Here, we show that injection of CL100 (also called MAPK phosphatase-1) into immature oocytes inhibited progesterone-induced oocyte maturation as well as MAPK activation and that injection of mRNA encoding a constitutively active MAPKK induced activation of histone H1 kinase and germinal vesicle breakdown in the absence of progesterone. (elsevierpure.com)
  • Injection of recombinant STE11 protein (a yeast MAPKK kinase) also induced initiation of oocyte maturation. (elsevierpure.com)
  • These data support the idea that the MAPKK/MAPK cascade plays an important role in oocyte maturation. (elsevierpure.com)
  • These results are consistent with an idea that there may exist a positive feedback loop consisting of Mos, the MAPKK/MAPK cascade, and MPF, which may be important for the initiation of oocyte maturation induced by progesterone. (elsevierpure.com)
  • Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation. (nature.com)
  • CDC2/SPDY transiently associates with endoplasmic reticulum exit sites during oocyte maturation. (antikoerper-online.de)
  • Maturation enhancement by microinjection into Xenopus oocytes represents a useful novel assay for analyzing Abl activity. (torvergata.it)
  • 1998. Influence of organochlorine pesticides on maturation and post-fertilization development of bovine oocytes in vitro . (cdc.gov)
  • 1990) published that amino acid receptors expressed in Xenopus oocyte after " microinjection of mRNA from the leg of locust Schistocerca gregaria. (researchbib.com)
  • Microinjection of in vitro transcribed D2H cRNA into Xenopus oocytes induced uptake of cystine as well as dibasic and neutral amino acids in a pattern similar to that of rat D2 and rabbit rBAT. (jci.org)
  • The ability of MEF2 factors to activate transcription from the XMLC2 promoter is assayed using microinjection of oocytes. (ucl.ac.uk)
  • The Popeye domain containing (POPDC) genes encode sarcolemma-localized cAMP effector proteins. (imperial.ac.uk)
  • The expression of Popdc1-LacZ transgene as well as Popdc1 protein was elevated in The Popeye domain containing (Popdc) family is a highly evolutionary conserved gene family, which shows no homology to other genes. (uni-wuerzburg.de)
  • Among 41 genes tested, we identified one, encoding the transmembrane protein Otop1 that upon expression induced a proton current in both Xenopus oocytes and HEK-293 cells. (hhs.gov)
  • Reduction of ribosomal RNA synthesis and ribosomal RNA genes in a mutant of Xenopus laevis which organizes only a partial nucleolus. (wikidata.org)
  • Expression of the mutated genes in xenopus oocytes has provided some insight into how the potassium channel mutation leads to lowering of the seizure threshold. (medscape.com)
  • reported that Xenopus oocytes translate foreign mRNA. (researchbib.com)
  • 1981) demonstrated the presence of binding sites for a-bungarotoxin when oocytes were injected with mRNA from the electric organ of Torpedo marmorata. (researchbib.com)
  • Interestingly, injection of the active MAPKK mRNA or the STE11 protein resulted in induction and accumulation of Mos protein. (elsevierpure.com)
  • We have analyzed the expression and intracellular distribution, during oogenesis and embryogenesis, of Vg1 RBP, a protein implicated in the intracellular localization of Vg1 mRNA to the vegetal cortex of Xenopus oocytes. (zfin.org)
  • Here we show that the activation kinetics of I sK expressed in Xenopus oocytes depend upon the amount of its mRNA injected, with larger amounts resulting in slower activation kinetics with a longer initial delay during activation. (wustl.edu)
  • Xenopus oocytes have been extensively used to study biogenesis, functional architecture, and modulation of plasma membrane expression of mRNAs encoding membrane receptors or ion channels. (researchbib.com)
  • 1982) presented the first electrophysiological evidence for the expression of functional nAChR in Xenopus oocytes. (researchbib.com)
  • RNA derived from alpha-L1 cDNA when injected alone in Xenopus oocyte resulted in the expression of functional nAChR. (researchbib.com)
  • Indeed, most live cell applications of PTS utilize single split inteins for the purpose of N/C-terminal tagging 16 - 18 or manipulating protein assembly/expression 19 , 20 . (biorxiv.org)
  • The purpose of heterologous expression is often to determine the effects of mutations and differential interactions on protein function. (wikipedia.org)
  • green fluorescent protein (GFP) fusions revealed egl-2 expression in a subset of neurons and muscles that could explain the mutant behavioral defects. (jneurosci.org)
  • Although Vg1 RBP RNA is detected in all blastomeres of the early embryo, the expression pattern in the one day old zebrafish embryo is almost identical to that of the equivalent stage Xenopus embryo. (zfin.org)
  • These results indicate that the zygotic expression pattern is similar in frogs and fish, and that there is a conserved zygotic expression of Vg1 RBP distinct from its expression in the oocyte. (zfin.org)
  • To elucidate the structure and properties of this channel, we used Xenopus oocytes for expression cloning of two functional complementary DNAs encoding an avermectin-sensitive glutamate-gated chloride channel. (nih.gov)
  • The expression of Popdc1-LacZ transgene as well as Popdc1 protein was elevated in the ventricular conduction system compared to the ventricular working myocardium. (uni-wuerzburg.de)
  • Transgenic frog technology indicates that the 3kb 5' proximal promoter fragment can direct cardiac muscle-specific reporter expression during Xenopus embryogenesis, reproducing the normal expression pattern of the endogenous gene. (ucl.ac.uk)
  • Deletion mapping of the 3kb XMLC2 promoter by Xenopus transgenesis defines the most proximal 708bp as the minimal promoter fragment sufficient to confer high level reporter expression in the embryonic heart. (ucl.ac.uk)
  • Expression of a 3kb XMLC2 promoter-CAT reporter gene introduced into the oocyte germinal vesicle is only detected in oocytes previously injected with MEF2D RNA, but not MEF2A. (ucl.ac.uk)
  • Using transgenic frog technology, I demonstrate that a 580bp cardiac α-actin promoter fragment drives high level expression of a GFP reporter in the heart and skeletal muscle of Xenopus embryos. (ucl.ac.uk)
  • Our recent work demonstrates that protein kinases can also modify intracellular trafficking of cardiac sodium channels to alter expression at the cell surface. (vanderbilt.edu)
  • It was shown that yolkin increased the proliferative activity of rat hippocampal precursor cells H19-7 cells and upregulated the expression/production of BDNF in a cyclic adenosine monophosphate (cAMP)-response element-binding protein (CREB)-dependent manner. (bvsalud.org)
  • A C. elegans messenger RNA expressed in Xenopus oocytes encodes an avermectin-sensitive glutamate-gated chloride channel. (nih.gov)
  • The D2H cDNA is 2284 nucleotides long and encodes a 663 amino acid protein that is 80% identical to the rat D2 amino acid sequence and 86% to that of the rabbit homologue rBAT. (jci.org)
  • The Xenopus cardiac myosin light chain 2 gene (XMLC2) encodes a contractile protein localised exclusively to the heart of the embryo. (ucl.ac.uk)
  • We report a complementary DNA sequence in the green alga Chlamydomonas reinhardtii that encodes a microbial opsin-related protein, which we term Channelopsin-1. (scienceopen.com)
  • We find that the electrophysiological and structural properties of these proteins indicate that they are new members of the ligand-gated ion channel superfamily. (nih.gov)
  • Electrophysiological studies in Xenopus oocytes for the opening of Escherichia coli SecA-dependent protein-conducting channels. (umassmed.edu)
  • We undertook electrophysiological assessment of mutant KCNT1 channels in a Xenopus oocyte model system. (bbk.ac.uk)
  • 1986). A Xenopus oocyte can also assemble the subunits of a receptor and insert them into its surface membrane (Parker et al. (researchbib.com)
  • Here we present a new approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into soluble and membrane proteins expressed in eukaryotic cells. (biorxiv.org)
  • We thus sought to complement these approaches with a method that could incorporate synthetic peptides carrying multiple post-translational modifications (PTMs) or ncAAs into both cytosolic and membrane proteins in live eukaryotic cells. (biorxiv.org)
  • Shr is a complex, membrane-anchored protein, with a unique N-terminal domain (NTD) and two NEAT domains separated by a central leucine-rich repeat region. (tcdb.org)
  • Heme is then conveyed to the SiaABC proteins (TC# 3.A.1.14.10) for transport across the membrane. (tcdb.org)
  • In the present study it is shown that Popdc1 protein in the heart was predominantly localized to the intercalated disc, lateral membranes and T-tubularsystem, where it was co-localized with other cardiac membrane proteins such as Cav1.2, Caveolin 3 and NCX1. (uni-wuerzburg.de)
  • Mechanosensitive membrane proteins: Usual and unusual suspects in mediating mechanotransduction. (stanford.edu)
  • This Viewpoint, which accompanies a Special Issue focusing on membrane mechanosensors, discusses unifying and unique features of both established and emerging mechanosensitive (MS) membrane proteins, their distribution across protein families and phyla, and current and future challenges in the study of these important proteins and their partners. (stanford.edu)
  • MS membrane proteins are essential for tissue development, cellular motion, osmotic homeostasis, and sensing external and self-generated mechanical cues like those responsible for touch and proprioception. (stanford.edu)
  • As the field continues to grow, so too will the list of proteins suspected to function as mechanosensors and the diversity of known MS membrane proteins. (stanford.edu)
  • a member of the plasma membrane intrinsic protein subfamily PIP1. (or.jp)
  • localizes to the plasma membrane and exhibits water transport activity in Xenopus oocyte. (or.jp)
  • The bound complex is internalized and translocates into the oocyte nucleus thanks to an SV40 nuclear localization signal, enhanced by an adjacent casein kinase phosphorylation site. (biologists.com)
  • We aim to study the coupling between LTCC and β adrenergic receptors in different cardiomyocyte microdomains, the distinct involvement of PKA and CAMKII (Ca2+/calmodulin-dependent protein kinase II) and explore how this functional complex is disrupted in heart failure.Methods:Global signaling between LTCCs and β adrenergic receptors was assessed with whole-cell current recordings and western blot analysis. (imperial.ac.uk)
  • They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. (antibodies-online.com)
  • Deregulated activity of the Abl protein tyrosine kinase is oncogenic in humans and in animals. (torvergata.it)
  • It was also determined that upregulation of CREB phosphorylation by yolkin is dependent on cyclic adenosine monophosphate/protein kinase A (cAMP/PKA) and phosphoinositide 3-kinases/protein kinase B (PI3K/Akt) signaling pathway activation. (bvsalud.org)
  • Injected intravenously into adult Xenopus females, the protein-DNA complex is rapidly transported through the bloodstream and, within the ovary, the vitellogenin ligand present in the protein binds to the receptors at the surface of the oocytes. (biologists.com)
  • 1987) reported for the first time that Xenopus oocytes successfully express glutamate receptors from lobster (Palinurus japonicus ) abdominal muscle. (researchbib.com)
  • Though researchers' attention and this Viewpoint focus on a few famous ion channels that are considered the usual suspects as MS mechanosensors, we also discuss some of the more unusual suspects, such as G-protein coupled receptors. (stanford.edu)
  • 2010 ) suggested that Shr is the prototype of a new group of NEAT composite proteins involved in haem uptake found in Pyogenic streptococci and Clostridium novyi . (tcdb.org)
  • The cardiac α-actin gene is a contractile protein expressed concomitantly in the somitic and cardiac muscle during Xenopus embryogenesis. (ucl.ac.uk)
  • Studies using Xenopus cell-free extracts, oocytes, eggs, embryos, larval stages, and adult frogs have yielded important insights into a multitude of key biological processes-from mechanisms underlying the cell cycle to embryonic development to human disease. (cshlpress.com)
  • We insert synthetic peptides into proteins of interest via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating different aspects of GFP, Na V 1.5 and P2X2 receptor function. (biorxiv.org)
  • The hemolytic Group A Streptococcus (GAS) possesses the Shr protein which as noted above, participates in iron acquisition by obtaining heme from host hemoglobin and delivering it to the adjacent receptor on the surface, Shp. (tcdb.org)
  • Because issues of receptor reserve complicate estimates of agonist efficacy and potency, we also examined the agonist actions of salvinorin A by measuring potassium conductance through G protein-gated K(+) channels coexpressed in Xenopus oocytes, a system in which receptor reserve is minimal. (erowid.org)
  • Jiang Y, Cheng Z, Mandon EC, Gilmore R. An interaction between the SRP receptor and the translocon is critical during cotranslational protein translocation. (umassmed.edu)
  • It has been recently shown that mutations of lens gap junction proteins are associated with certain forms of inherited congenital cataract. (rosalindfranklin.edu)
  • Most are missense mutations but there is one report of a premature stop codon, resulting in a Kv1.1 protein that is truncated in the C-terminal (Kv1.1ΔC79). (biomedcentral.com)
  • Some of these loci have been identified further as specific mutations in the KCNQ2 and KCNQ3 M-type potassium channel proteins. (medscape.com)
  • Semi-synthetic approaches offer an alternative means to manipulate proteins post-translationally, but these modifications have typically been performed in vitro 3 - 8 . (biorxiv.org)
  • To investigate the mechanism of the egl-2(gf) phenotype and rescue by imipramine, we analyzed EGL-2 currents in Xenopus oocytes. (jneurosci.org)
  • MCEPH can form tetramers with Kv1.1 in cell culture and has a dominant negative effect on Kv1.2 and Kv1.3 currents in oocytes. (biomedcentral.com)
  • Chemical or genetic engineering of proteins provides great potential to study protein function and pharmacology or to generate proteins with novel properties. (biorxiv.org)
  • Where possible, we performed homology modelling to predict putative effects of variants on protein structure and function. (bbk.ac.uk)
  • The hydrophobic core region of the protein shows homology to the light-activated proton pump bacteriorhodopsin. (scienceopen.com)
  • There have been three types of gap junctional proteins (connexins) identified in the mammalian lens: Cx43, Cx46, Cx50. (rosalindfranklin.edu)
  • In contrast to versions of Abl capable of transforming mammalian cells, which were highly active when introduced into oocytes, the activity of wild type c-Abl was inhibited. (torvergata.it)
  • Altogether, this suggests that an SH3 domain-dependent Abl regulation mechanism similar to the one observed in mammalian cells operates in Xenopus oocytes. (torvergata.it)
  • Mandon EC, Trueman SF, Gilmore R. Protein translocation across the rough endoplasmic reticulum. (umassmed.edu)
  • Trueman SF, Mandon EC, Gilmore R. Translocation channel gating kinetics balances protein translocation efficiency with signal sequence recognition fidelity. (umassmed.edu)
  • Group A Streptococcus (GAS), a β-haemolytic human pathogen, expresses a NEAT protein, Shr, which binds several haemoproteins and extracellular matrix (ECM) components. (tcdb.org)
  • One of the major aims of Dr. Ebihara's research project are to obtain a more detailed understanding of the biophysical properties of cloned lens gap junctional proteins and to localize the molecular determinants of permeability and gating of the gap junctional channels. (rosalindfranklin.edu)
  • There are protocols for analyses of Xenopus immunology, neurobiology, metamorphosis, and adult physiology. (cshlpress.com)
  • Site-directed mutagenesis was utilised to map the domains involved in protein-protein interaction. (imperial.ac.uk)
  • Biochemical examinations of Popdc1 showed that Popdc1 is a transmembrane protein. (uni-wuerzburg.de)
  • Because the approach can introduce virtually any chemical modification into both intracellular and extracellular regions of target proteins, we anticipate that it will overcome some of the drawbacks of other semi-synthetic or ribosome-dependent methods to engineer proteins. (biorxiv.org)
  • Additional studies of agonist potency and efficacy of salvinorin A, performed by cotransfecting either the chimeric G proteins Gaq-i5 or the universal G protein Ga16 and quantification of agonist-evoked intracellular calcium mobilization, affirmed that salvinorin A was a potent and effective kappa-opioid agonist. (erowid.org)
  • Circulating hormones that activate intracellular signaling cascades, and ultimately protein kinases, can rapidly regulate ion channel function. (vanderbilt.edu)
  • Members of the frog Xenopus genus are versatile model organisms used in developmental biology, cell biology, neuroscience, and toxicology research, among others. (cshlpress.com)
  • The predicted truncated Kv1.1 protein (MCEPH) will retain only the N-terminal (T1) domain, the first transmembrane domain (S1) and the first extracellular loop. (biomedcentral.com)
  • Microbial rhodopsins (Rhs) are transmembrane proteins that utilize light-induced isomerization of their retinal cofactor to function as light-sensitive ion channels, pumps and sensors. (elifesciences.org)
  • The substituted amino acid is located within the second C2HC motif in the conserved zinc finger domain of NANOS3 and in silico molecular modelling suggests destabilization of protein-RNA interaction. (hindawi.com)
  • The interaction of POPDC proteins was investigated by co-precipitation, proximity ligation, bioluminescence resonance energy transfer and bimolecular fluorescence complementation. (imperial.ac.uk)
  • Both imply an interaction among individual channel proteins during IsK activation. (wustl.edu)
  • In this paper we define the mechanism of inactivation for sqKv1A channels in Xenopus oocytes to investigate these and other discrepancies. (nih.gov)
  • Inactivation of sqKv1A in Xenopus oocytes was found to be unaffected by genetic truncation of the N-terminus, but highly sensitive to certain amino acid substitutions around the external mouth of the pore. (nih.gov)
  • External TEA and K(+) ions slowed inactivation of sqKv1A channels in oocytes, and chloramine T (Chl-T) accelerated inactivation. (nih.gov)
  • The newly developed oocyte shuttle protein contains a streptavidin moiety that tightly binds biotinylated DNA. (biologists.com)
  • The increase in Mos protein and the activation of MAPK by injecting cyclin A protein into immature oocytes were both blocked also by cycloheximide treatment. (elsevierpure.com)
  • The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. (antibodies-online.com)
  • The described setup and experimental design enable real-time monitoring of substrate turnover in light-activated enzymes on a molecular scale, thus opening the pathway to a deeper understanding of enzyme activity and protein-protein interactions. (elifesciences.org)
  • We have tested the activity of human c-Abl microinjected into oocytes of Xenopus laevis. (torvergata.it)