• The protein expression levels of phosph-protein kinase B (P-AKT), phosph-mammalian targets of rapamycin (P-mTOR), and phosph-p70 ribosomal s6 kinase (P-p70s6K) in tumor cells were likewise reduced after hinokitiol treatment. (jcancer.org)
  • A Central Role for Neuronal AMP-Activated Protein Kinase (AMPK) and Mammalian Target of Rapamycin (mTOR) in High-Protein Diet-Induced Weight Loss. (diabetesjournals.org)
  • mTORC2 promoted phosphorylation of protein kinase B (PKB, or Akt) and PKC, Akt activity, and nuclear NF-kappaB transcription factors in response to T cell activation. (omicsdi.org)
  • Protein kinase C (PKC) is involved in a wide array of cellular processes such as cell proliferation, differentiation and apoptosis. (omicsdi.org)
  • The compound is an inhibitor of mammalian protein kinase and has been applied topically for the treatment of skin metastasis showing good local tolerability [5]. (who.int)
  • The toxicity data of protein kinase inhibitors in humans is also well established [5]. (who.int)
  • For the recombinant protein purifications we implement a large array of chromatographic techniques. (embl.org)
  • BioIntron recombinant protein expression platform can provide high-quality protein in a flexible, cost-effective manner, in the least amount of time. (biointron.com)
  • Market growth has been supported by advances in recombinant protein production technologies, including the engineering of expression hosts, the optimization of upstream cultivation (e.g., bioreactor design, nutritional, and physical parameters), and the development of more efficient protein extraction and purification methods. (frontiersin.org)
  • Finally, we describe methods for the growth of these cells lines in scalable suspension culture for the preparation of milligram amounts of recombinant protein. (essex.ac.uk)
  • Our results lend support to the concept of post-transcriptional RNA operons, but we further present evidence that nuclear proximity of genes may provide substantial functional regulation in mammalian single cells. (nih.gov)
  • Expression levels of PER2, a clock protein, were elevated in Sik3 -knockdown cells but down-regulated in Sik3 -overexpressing cells, which could be attributed to a phosphorylation-dependent decrease in PER2 protein stability. (elifesciences.org)
  • When inducing long-term potentiation (LTP) in the hippocampus, we observed an increase of Arc protein highly correlated with an increase in fluorescent intensity and the number of mCherry-positive cells. (lu.se)
  • The presence of plasma membrane multidrug resistance protein P-glycoprotein (P-gp) is favorable for tumor cells to elicit chemotherapeutic resistance. (jcancer.org)
  • PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization. (embl.org)
  • The facility expresses and purifies proteins from E. coli , insect cells and mammalian cells. (embl.org)
  • One of the known apoptotic pathways in mammalian cells involves release of mitochondrial Cytochrome c into the cytosol. (uni-frankfurt.de)
  • 1997). The screen resulted in the identification of six proteins displaying cell death-inhibiting activity in S. pombe as well as anti-apoptotic potential in mammalian cells. (uni-frankfurt.de)
  • Examples of essential proteins are antibodies defending our body against foreign invaders and hemoglobulin responsible for importing oxygen to our cells and exporting carbon dioxide out from our cells. (kth.se)
  • Our first tau protein expressed in mammalian cells. (bonopusbio.com)
  • VCP is one of the most abundant cytoplasmic proteins in eukaryotic cells. (wikipedia.org)
  • In mammalian cells, VCP is predominantly localized to the cytoplasm, and a significant fraction is associated to membranes of cellular organelles such as the endoplasmic reticulum (ER), Golgi, mitochondria, and endosomes. (wikipedia.org)
  • Addition of the Wnt3a ligand to rat brain membranes or cultured cells elicits Fz-dependent guanine-nucleotide exchange on Go/i proteins. (uni-konstanz.de)
  • Here, we have developed a new method which uses these cylindrical structures as platforms to detect protein interactions in cells. (hal.science)
  • To test the reliability of this detection scheme, we used it to probe the interactions among three mRNA-binding proteins in both fixed and living cells and compared the results to those obtained by pull-down assays. (hal.science)
  • Altogether, the results indicate that microtubules can be used as platforms to detect protein interactions in mammalian cells, which should provide a basis for investigating pathogenic protein interactions involved in human diseases. (hal.science)
  • Over the last three decades, the expression of recombinant proteins in plants and plant cells has been promoted as an alternative cost-effective production platform. (frontiersin.org)
  • Most recombinant proteins are currently produced in prokaryotic cells (mainly the bacterium Escherichia coli ) and a small number of well-characterized mammalian cell lines, such as Chinese hamster ovary (CHO) cells. (frontiersin.org)
  • There are also several platforms based on plants and plant cells, but these have not been included in the latest market studies, indicating they have not yet commanded a significant share of commercial protein production capacity. (frontiersin.org)
  • We, for the first time, have studied plutonium-binding proteins using a metalloproteomic approach with rat PC12 cells. (northwestern.edu)
  • Our results show that several proteins from PC12 cells show affinity towards Pu 4+ -NTA (plutonium bound to nitrilotriacetic acid). (northwestern.edu)
  • A novel 5S RNA-protein (RNP) complex in human and mouse cells has been analyzed using patient autoantibodies. (nih.gov)
  • It reproduces protein time courses in wild-type cells, mimics correctly the phenotypes of many mutant strains, and predicts the phenotypes of currently uncharacterized mutants. (ebi.ac.uk)
  • Chromosomal double-strand breaks (DSBs) in mammalian cells are repaired by either homology-directed repair (HDR), using a homologous sequence as a repair template, or nonhomologous end-joining (NHEJ), which often involves sequence alterations at the DSB site. (arizona.edu)
  • Cells need ribosomes in order to create proteins. (europa.eu)
  • This second project involved the protein Maf1, which inhibited Pol III activity in yeast and mammalian cells. (europa.eu)
  • Indeed, despite the importance of Maf1, the study of this protein in mammalian cells was just beginning by the time of the project elaboration. (europa.eu)
  • Firstly, we discovered a major difference between the regulation of Pol I in yeast and mammalian cells. (europa.eu)
  • Mammalian aging is regarded as partially due to the reduced capacity of stem/precursor cells to endure self-renewing divisions. (ampkpathway.com)
  • Utilizing Rictor knock-out cells, immunoprecipitation and mass spectrometry, a novel Rictor associated protein, heterogeneous nuclear ribonucleoprotein M (hnRNP M), was identified. (omicsdi.org)
  • Here, we explored differences in the cellular response of both mammalian and tick cells to nairovirus infection using Hazara virus (HAZV), which is a close relative of CCHFV within the CCHFV serogroup. (liverpool.ac.uk)
  • In addition, we show that the HAZV nucleocapsid (N) protein was abundantly cleaved by caspase 3 in these mammalian cells at a conserved DQVD motif exposed at the tip of its arm domain, and that cleaved HAZV-N was subsequently packaged into nascent virions. (liverpool.ac.uk)
  • However, in stark contrast, we show for the first time that nairovirus infection of cells of the tick vector failed to induce apoptosis, as evidenced by undetectable levels of cleaved caspases and lack of cleaved HAZV-N. Our findings reveal that nairoviruses elicit diametrically opposed cellular responses in mammalian and tick cells, which may influence the infection outcome in the respective hosts. (liverpool.ac.uk)
  • Hassa, P.O., Haenni, S.S., Elser, M. & Hottiger, M.O. Nuclear ADP-ribosylation reactions in mammalian cells: where are we today and where are we going? (nature.com)
  • The findings also reveal that the selective distribution of the three proteins was altered during development presumably to meet the changing needs of the cells to maintain normal and functional levels of iron and other essential metals. (cdc.gov)
  • Both proteins are also involved in stopping cell division in older cells (senescence). (medlineplus.gov)
  • The p14(ARF) and p53 proteins are often made in cells that are unable to undergo cell division. (medlineplus.gov)
  • Mammalian cells produce PGs, which are involved in biological processes such as cell growth and differentiation. (lu.se)
  • Mammalian target of rapamycin protein complex 2 regulates differentiation of Th1 and Th2 cell subsets via distinct signaling pathways. (omicsdi.org)
  • Many functions of the mammalian target of rapamycin (mTOR) complex 1 (mTORC1) have been defined, but relatively little is known about the biology of an alternative mTOR complex, mTORC2. (omicsdi.org)
  • Mammalian target of rapamycin (mTOR) plays a range of crucial roles in cell survival, growth, proliferation, metabolism, and morphology. (omicsdi.org)
  • Here, we show that the mammalian target of rapamycin complex 2 (mTORC2) has an important function in phosphorylation of both TM and HM in all conventional PKCs, novel PKCepsilon as well as Akt. (omicsdi.org)
  • By far the greatest demand for recombinant mammalian proteins is for therapeutic development and applications. (biointron.com)
  • The demand for recombinant proteins is therefore rising steadily, with a market valued at US$1.654 billion in 2017 predicted to reach US$2.850 billion by 2022 ( Markets and Markets, 2017 ). (frontiersin.org)
  • Tail-anchored (TA) proteins are ubiquitous in eukaryotes and fulfill various important functions in the cell, such as the mediation of cell-cell contact and apoptosis signaling. (caltech.edu)
  • The identified plutonium interacting proteins also have functional roles in downregulation of apoptosis and other pro-proliferative processes. (northwestern.edu)
  • We further found that the level of Bcl-xL protein, which has been shown to be able to inhibit apoptosis, was increased in H1299 cell lines (both p53-null and p53 positive) infected by T3D, but decreased in the same cell lines infected by T1L. (umanitoba.ca)
  • Mammalian apoptosis-inducing protein, HAP, induces bacterial cell death. (bvsalud.org)
  • In attempting to produce the HAP, endoplasmic reticulum (ER) targeted apoptosis -inducing protein , as a GST-fusion protein we found that the expression of HAP, but not GST alone, induced bacterial cell death . (bvsalud.org)
  • When this decoy receptor is engaged by a ligand to the displayed protein/peptide, the receptor expressing cell is rescued from apoptosis. (lu.se)
  • The p53 protein is an important tumor suppressor that is essential for regulating cell division, senescence, and self-destruction (apoptosis). (medlineplus.gov)
  • article{3ef64d42-e741-442e-a1b5-bd0843c8bfaa, abstract = {{The activity-regulated cytoskeleton-associated (Arc) protein is essential for synaptic plasticity and memory formation. (lu.se)
  • The history of protein dynamics traces back to 1935, when Schoenheimer and Rittenberg synthesized the first isotopologs of biological molecules to demonstrate the continuous renewal of proteins throughout life 14 , 15 . (nature.com)
  • In contrast to steady-state protein abundance, which may be quantified directly in mass spectrometry (MS) by spectral intensity 16 , 17 or sampling frequency 18 , 19 , protein turnover rates cannot be predicted from steady-state data 20 , requiring instead methods that can distinguish old and new protein molecules in mass spectra 11 , 21 . (nature.com)
  • To enable the tracking of Arc molecules from individual neurons in vivo, we devised an adeno-associated virus (AAV) mediated approach to tag the N-terminal of the mouse Arc protein with a fluorescent reporter using CRISPR/Cas9 homologous. (lu.se)
  • To enable the tracking of Arc molecules from individual neurons in vivo, we devised an adeno-associated virus (AAV) mediated approach to tag the N-terminal of the mouse Arc protein with a fluorescent reporter using CRISPR/Cas9 homologous independent targeted integration (HITI). (lu.se)
  • Its main function is to segregate protein molecules from large cellular structures such as protein assemblies, organelle membranes and chromatin, and thus facilitate the degradation of released polypeptides by the multi-subunit protease proteasome. (wikipedia.org)
  • Proteins are therefore molecules of great complexity and near infinite diversity, making them suitable for many different applications. (frontiersin.org)
  • I are the book mammalian protein metabolism volume iii and critical masculinity of it. (rankine-mfg-co.com)
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  • In this study, we have structurally and biochemically characterized Bag6 and Ubl4Apreviously shown to be crucial in the recognition and delivery TA proteins from the ribosome to TRC40. (caltech.edu)
  • Using an integrated platform of metabolic labeling, high-resolution mass spectrometry and computational analysis, we report here a comprehensive dataset of the in vivo half-life of 3,228 and the expression of 8,064 cardiac proteins, quantified under healthy and hypertrophic conditions across six mouse genetic strains commonly employed in biomedical research. (nature.com)
  • The dataset contains over 1.92 million data points in protein isotope labeling kinetics, culminating as the in vivo turnover rates of 3,228 cardiac proteins and the expression levels of 8,064 proteins. (nature.com)
  • We also maintain an extensive collection of expression vectors and bacterial strains and we have various frequently used proteins such as for example proteases and polymerases readily available. (embl.org)
  • We maintain a large database of expression vectors at the EMBL Protein Expression and Purification Core Facility. (embl.org)
  • Moreover, expression studies on mRNA and protein level showed upregulation of Fte-1/S3a in colon, lung and kidney carcinoma. (uni-frankfurt.de)
  • The results presented in this thesis are the outcome of five different studies aiming to improve and balance the expression levels of recombinant proteins in mammalian cell lines. (kth.se)
  • The fourth study generated an easy-to-use toolbox for balancing the expression levels of recombinant proteins in mammalian cell lines. (kth.se)
  • However, the market is still dominated by prokaryotic and mammalian expression systems, the former offering high production capacity at a low cost, and the latter favored for the production of complex biopharmaceutical products. (frontiersin.org)
  • Here, we discuss the strengths of plant expression systems for specific applications, but mainly address the bottlenecks that must be overcome before plants can compete with conventional systems, enabling the future commercial utilization of plants for the production of valuable proteins. (frontiersin.org)
  • Even so, plants as an alternative expression platform offer unique advantages, particularly when target proteins are difficult to produce in conventional systems, require specific qualitative properties such as particular glycan profiles, and/or must be produced on a larger scale in response to urgent demand. (frontiersin.org)
  • The large-scale expression of many membrane proteins, including the members of the G protein-coupled receptor superfamily, in a correctly folded and fully functional form remains a formidable challenge. (essex.ac.uk)
  • We found that the expression of Bcl-xL protein in H1299 cell line infected by MRV was majorly controlled by the S1 gene segment which encodes the σ1 cell attachment protein and the σ1s non structural protein, while minorly controlled by L3 gene segment of MRV. (umanitoba.ca)
  • The HAP protein inhibited the bacterial growth within 30 min after inducting HAP expression. (bvsalud.org)
  • In this study, we have characterized a novel approach for identification and selection of protein-protein interactions, denoted SPIRE (selection of protein interactions by receptor engagement), which is based on a mammalian expression system. (lu.se)
  • MitoCarta3.0 is an inventory of 1136 human and 1140 mouse genes encoding proteins with strong support of mitochondrial localization, now with sub-mitochondrial compartment and pathway annotations. (broadinstitute.org)
  • To generate this inventory, we performed mass spectrometry of mitochondria isolated from fourteen tissues, assessed protein localization through large-scale GFP tagging/microscopy, and integrated these results with six other genome-scale datasets of mitochondrial localization, using a Bayesian approach. (broadinstitute.org)
  • The subcellular localization of CDC48 has not been fully characterized, but is likely to be similar to the mammalian counterpart. (wikipedia.org)
  • Prior studies in our laboratory examined the localization and developmental changes of DMT1 in rat cochlea and since the two Zip proteins are also likely to contribute to the transport of essential and non-essential divalent cations, we performed immunolabeling experiments in postnatal day three rat pups and adult rats. (cdc.gov)
  • Proteins with quantified dynamics belong to over 10 major cellular compartments and over 200 known pathways. (nature.com)
  • Key proteins in mitochondria and metabolic pathways are encompassed, in addition to contractile machineries and sarcolemmal signaling proteins. (nature.com)
  • The protein moiety of the 5S RNP has been identified as ribosomal protein L5, which is known to be released from ribosomes in a complex with 5S after various treatments of the 60S subunit. (nih.gov)
  • The ribosomal proteins are produced in a two-step process, the first being transcription by Pol II. (europa.eu)
  • These responses were sensitive to a Wnt antagonist and to pertussis toxin, which decouples the Go/i proteins from their receptors through covalent modification. (uni-konstanz.de)
  • Background The functional interchangeability of mammalian Notch receptors (Notch1-4) in normal and pathophysiologic contexts such as for example cancer is unsettled. (careersfromscience.org)
  • A combination of immobilized metal ion chromatography, 2D gel electrophoresis, and mass spectrometry was employed to analyze potential plutonium-binding proteins. (northwestern.edu)
  • We have demonstrated proof of concept by creating a general plasma membrane bound decoy receptor, by displaying a protein or a peptide genetically fused to a trunctated version of the CD40 molecule. (lu.se)
  • For academic research groups, recombinant mammalian proteins will be required for functional and high-resolution structure determination. (biointron.com)
  • Mammalian cell lines are often the preferred cell factories for manufacturing biologics since they generate proteins with human-like post-translational modifications, which are often essential features to obtain functional, safe, and effective therapeutics. (kth.se)
  • The interaction of DNA ligase III with its protein partner, XRCC1, in BER was the first example of a functional interaction between BRCT modules, and the 3D structure of this XRCC1 BRCT domain has been solved. (ucl.ac.uk)
  • Most of these mutations lead to production of little or no functional p16(INK4A) protein. (medlineplus.gov)
  • Nevertheless, evidence for intercellular transport of Arc in the mammalian brain is still lacking. (lu.se)
  • This is the first study to provide support for inter-neuronal in vivo transfer of Arc in the mammalian brain. (lu.se)
  • Consequently, mutations leading to dysfunctional proteins is the cause of many known diseases. (kth.se)
  • The consequences of mutations on protein folding were also assessed. (ucl.ac.uk)
  • The CDKN2A gene mutations found in melanoma result in a nonfunctional p16(INK4A) protein. (medlineplus.gov)
  • Together, the germline and somatic mutations impair the function of proteins that regulate division and senescence, leading to uncontrolled cell growth and the formation of a melanoma. (medlineplus.gov)
  • We also offer biophysical support for protein quality control, protein characterization and various types of interaction studies. (embl.org)
  • Protein stability is a major regulatory principle of protein function and cellular homeostasis. (nature.com)
  • In mammals the cellular form of the prion protein (PrP C ) is a ubiquitous protein involved in many relevant functions in the central nervous system. (sissa.it)
  • We find that nuclear organization has the greatest impact, and that genes encoding for physically interacting proteins specifically tend to covary, suggesting importance for protein complex formation. (nih.gov)
  • With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)
  • however, Fz proteins constitute a distinct group within the GPCR (G-proteincoupled receptor) superfamily, and Fz signalling can be G-proteinindependent in some experimental setups, leading to concerns about the GPCR nature of these proteins. (uni-konstanz.de)
  • More than 300 protein-based medicines have been approved in the USA and Europe, and proteins account for almost a third of all pharmaceuticals in development ( Walsh, 2018 ). (frontiersin.org)
  • DNA repair processes employ numerous proteins whose efficient function depends on interactions with each other. (ucl.ac.uk)
  • The major pathway that corrects endogenous DNA damage is base-excision repair (BER), which is mediated by sequential pair-wise protein interactions. (ucl.ac.uk)
  • Far Western, co-affinity, co-immunoprecipitation and yeast two- hybrid analyses were employed to identify protein: protein interactions that might confirm this role. (ucl.ac.uk)
  • This method requires only a conventional optical microscope and straightforward fluorescence image analysis for detection and quantification of protein interactions. (hal.science)
  • We also tested whether the molecular interactions of Cx43, a membrane protein, can be investigated with this system. (hal.science)
  • This demonstrates that the approach may serve as a tool in post-genomic research for identifying protein-protein interactions, without prior knowledge of either component. (lu.se)
  • The Arc gene, which contains remnants of a structural GAG retrotransposon sequence, produces a protein that self-assembles into capsid-like structures harboring Arc mRNA. (lu.se)
  • The 'BRCT' domain, first noted in the C-terminus of the product of the BRCA1 breast cancer suppressor gene (BRCA1 Carboxy Terminal), has been identified in~50 proteins involved in DNA repair/recombination/cell cycle control. (ucl.ac.uk)
  • the alphabet is the 20 proteinogenic amino acids for proteins and the sense codons (i.e., the 61 codons that encode amino acids in the standard genetic code ) for aligned protein-coding gene sequences. (wikipedia.org)
  • Fifty four T1L×T3D reassortants were used to map which gene or gene combination was responsible for the changes of Bcl-xL protein. (umanitoba.ca)
  • Neither sister-chromatid exchange nor gene-targeting frequencies show a dependence on these NHEJ proteins. (arizona.edu)
  • A different type of alteration involving the CDKN2A gene can result in reduced amounts or an absence of the p16(INK4A) or p14(ARF) protein. (medlineplus.gov)
  • 1997 Individual and mouse had been cloned into mammalian appearance vectors with Myc (computers2+) FLAG (pFLAG-CMV2 Sigma) or GFP (pEGFPC1 Invitrogen) tags. (techuniq.com)
  • An IRP-like protein from Plasmodium falciparum binds to a mammalian ir" by Mark Loyevsky, Timothy LaVaute et al. (howard.edu)
  • The p16(INK4A) protein attaches (binds) to two other proteins called CDK4 and CDK6. (medlineplus.gov)
  • Detailed comparisons of the plant and mammalian HPPD-ligand structures suggest a structural basis for the high degree of plant selectivity of certain HPPD inhibitors and point to design strategies to obtain potent and selective inhibitors of plant HPPD as agrochemical leads. (rcsb.org)
  • EC 1.13.11.27) from the plant Arabidopsis thaliana with and without an herbicidal benzoylpyrazole inhibitor that potently inhibits both plant and mammalian HPPDs. (rcsb.org)
  • We also determined the structure of a mammalian (rat) HPPD in complex with the same nonselective inhibitor. (rcsb.org)
  • Therapeutic proteins (e.g., antibodies, vaccines, enzymes, cytokines, and growth factors) account for almost half of this market, followed by industrial proteins (e.g., technical enzymes) and research reagents (e.g., antibodies for protein detection and purification) ( Markets and Markets, 2017 ). (frontiersin.org)
  • Abcam: antibodies, proteins, kits. (abcam.com)
  • In fact, substitution models can be developed for any biological characters that can be encoded using a specific alphabet (e.g., amino acid sequences combined with information about the conformation of those amino acids in three-dimensional protein structures [7] [8] ). (wikipedia.org)
  • Information on global protein dynamics therefore has the potential to expand the depth and scope of disease phenotyping and therapeutic strategies. (nature.com)
  • Recent biochemical and structural studies have helped us identify and elucidate the details of the TA protein targeting pathway in yeast, also known as the Guided Entry of TA protein (GET) pathway. (caltech.edu)
  • Cyt c then together with ATP or dATP induces a conformational change in the adaptator protein Apaf-1 (a homologue of the C. elegans CED4 protein) (Zou, Henzel et al. (uni-frankfurt.de)
  • 2008). A mitochondrial protein compendium elucidates complex I disease biology. (broadinstitute.org)
  • By proximity ligation assay (PLA), we demonstrated that the mCherry-Arc fusion protein retains the Arc function by interacting with the transmembrane protein stargazin in postsynaptic spines. (lu.se)
  • Coimmunoprecipitation and Traditional western blotting Co-immunoprecipitation between over-expressed protein had been performed utilizing a FLAG Tagged proteins Immunoprecipitation package (Sigma). (techuniq.com)
  • Many of them are important chaperones that regulate folding or unfolding of substrate proteins. (wikipedia.org)
  • For example, it can be stimulated by heat or by a putative substrate protein. (wikipedia.org)
  • In fact, the protein is a substrate in a process in which it converts into an infectious and pathological form denoted as prion. (sissa.it)
  • Proteins are the building blocks of all living organisms enabling us to function and survive. (kth.se)
  • Furthermore, we are an active member of a network of protein facilities across Europe called Protein Production and Purification Partnership in Europe ( P4EU ) to improve information exchange and the evaluation of novel technologies. (embl.org)
  • A search for the mammalian homolog of CDC48 (valosin) revealed a 97 kDa protein precursor named "valosin-containing protein (VCP)" or p97, and also showed that it was only generated as an artefact of purification rather than during physiological processing. (wikipedia.org)
  • The 5S RNA in the particle is matured at its 3' end, consistent with the results of in vivo pulse-chase experiments which indicate that this RNP represents a later step in 5S biogenesis than a previously described 5S*/La protein complex. (nih.gov)
  • Figure 1: Macrodomain-containing proteins recruit to sites of PARP1 activity in vivo . (nature.com)
  • Hassa, P.O. & Hottiger, M.O. The diverse biological roles of mammalian PARPS, a small but powerful family of poly-ADP-ribose polymerases. (nature.com)
  • For biological systems, Q peaks were first observed in protein from different perspectives [3,7,9,13,15,21-23] , the detailed molec- powders and attributed to 1H-14N spin pairs [9], which were also ular mechanism of the ubiquitous 1H-14N Q peaks in biological sys- thought to be of critical importance for the background 1H relaxa- tems has not been established. (lu.se)
  • Unlike many bacterial AAA+ proteins, assembly of VCP hexamer does not depend on the presence of nucleotide. (wikipedia.org)
  • COX-1 is expressed constitutively and is isolated throughout most cell lines in almost all mammalian tissues. (medscape.com)
  • It also is reported that anti-TAPA-1 induce protein tyrosine phosphorylation that is prevented by increased intercellular thiol levels. (techuniq.com)
  • The association between hnRNP M and Rictor was verified using recombinant and endogenous protein and the binding site was found to be within aa 1~532 of hnRNP M. The presence of hnRNP M significantly affects phosphorylation of SGK1 S422, but not of Akt S473, PKC? (omicsdi.org)
  • Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes. (nature.com)
  • The mechanism, which is based on the synthesis and degradation of three ''master regulator'' proteins (CtrA, GcrA, and DnaA), is converted into a quantitative model, in order to study the temporal dynamics of these and other cell cycle proteins. (ebi.ac.uk)
  • Previous reports have suggested that protein kinases play important roles in the regulation of circadian clocks ( Reischl and Kramer, 2011 ). (elifesciences.org)
  • In the first study, we investigated the productivity differences between mammalian cell lines from different origins. (kth.se)
  • In summary, this thesis provides strategies to improve the production process of biologics in mammalian cell lines and thereby contributes to the goal of offering safe, effective, and affordable medicine to patients in every part of this world. (kth.se)
  • In this chapter, we focus on the construction of stable mammalian cell lines to overcome this hurdle. (essex.ac.uk)
  • A similar change of Bcl-xL protein was not observed In HCT116 and HT1080 cell lines with MRV infection. (umanitoba.ca)
  • Quality control of protein reagents for the improvement of research data reproducibility. (embl.org)
  • Many other proteins are used as diagnostics or research reagents. (frontiersin.org)
  • The protein has a unique primary structure where the unstructured N-terminal moiety possesses characteristic sequences wherein histidines are able to coordinate metal ions, in particular copper ions. (sissa.it)
  • Regulation of proteome integrity requires chaperone-assisted folding of unfolded proteins, dissolution of misfolded aggregates, proteolytic removal of proteins, and other concerted proteostatic processes 1 , 2 . (nature.com)
  • A Data-Driven, Mathematical Model of Mammalian Cell Cycle Regulation. (ebi.ac.uk)
  • Because proteostatic events often trigger zero net change in protein abundance but instead alter protein temporal dynamics 11 - 13 , they typically elude conventional experiments that measure only the steady-state abundance of proteins. (nature.com)
  • Protein dynamics data are therefore sought to better describe homeostatic processes and enhance the utility of phenotyping-by-omics approaches. (nature.com)
  • However, large-scale protein dynamics datasets have remained scarce, due to the specialized technologies necessary to measure turnover of individual proteins on a global scale. (nature.com)
  • We report here a large dataset of protein turnover dynamics in the heart of six common genetic strains of mice, acquired under both normal and hypertrophic conditions. (nature.com)
  • In Leishmania infantum, the LiVCP protein is essential for the intracellular development of the parasite and its survival under heat stress. (wikipedia.org)
  • MetaCore™ analysis based on this group of proteins produced a pathway with a statistically significant association with development of neoplastic diseases. (northwestern.edu)
  • Fortunately, the advancement of modern medicine has enabled proteins also to be employed as therapeutics to treat and cure various conditions. (kth.se)
  • Open up in another window Amount 2 Mammalian ICNs Induce T Cell Advancement in the Bone tissue Marrow and in Fetal Thymic Body organ Cultures. (careersfromscience.org)